Various modified instruments are used for the anterior reconstruction of the tumor lesion affecting the second cervical vertebra, but there have been no reports regarding individual integrated artificial axis (IAA) prosthesis fabricated by selective laser melting. In the present work, a new type of IAA prosthesis has been designed with a 3-dimensional (3D) finite element model of normal occiput-the fourth cervical vertebra being established to assess its biomechanics. For easy comparison, another 3D finite element model is also established for the T-shaped Harms cage and an additional posterior fixation was performed on each model. The models are tested under a preliminary loading of 40 N to simulate cervical physical action including flexion, extension, lateral bending, and rotation. Under various loads from 4 different directions, the maximum stress and displacement of the IAA are less than those of the modified T-shaped Harms cage. Except for flexion, the maximum stress of the third cervical vertebra endplate of the IAA is smaller than that of the modified T-shaped Harms cage. The new prosthesis with axis is a good choice for upper cervical operation, which not only can greatly increase the operation stability of the upper cervical segment but also could significantly reduce the risk of fixation failure due to Harms cage subsidence.
Abstract Head and neck cancer is the 7th most common cancer in the world and the primary treatment is surgical resection. Optical probes have shown great potential for application in image‐guided surgery to delineate tumor margins more accurately. Special biomarkers that are aberrant in tumors are considered in tumor‐targeting strategies. Targeted fluorescent probes can enable accurate visualization of tumor margins with appropriate targeting strategies, thereby providing better sensitivity and specificity. In this review, the preclinical evidence on optical probes that have been developed with specific targeting strategies is summarized and challenges in the clinical translation of novel targeted fluorescent probes are discussed.
Neuropeptide Y (NPY) induced reentry of differentiated rat neonatal and adult cardiomyocytes into the cell cycle. NPY also induced differentiation of bone marrow-derived mesenchymal stem cells (MSC) into cardiomyocytes following transplantation into infarcted myocardium. Rat neonatal and adult cardiomyocytes were treated in vitro with vehicle, NPY, fibroblast growth factor (FGF; 100 ng/ml), or FGF plus NPY. DNA synthesis, mitosis, and cytokinesis were determined by immunocytochemistry. NPY-induced MSC gene expression, cell migration, tube formation, and endothelial cell differentiation were analyzed. Male rat green fluorescent protein-MSC (2 × 10 6 ), pretreated with either vehicle or NPY (10 −8 M) for 72 h, were injected into the border zone of the female myocardium following left anterior descending artery ligation. On day 30, heart function was assessed, and hearts were harvested for histological and immunohistochemical analyses. NPY increased 5-bromo-2′-deoxy-uridine incorporation and promoted both cytokinesis and mitosis in rat neonatal and adult myocytes. NPY also upregulated several genes required for mitosis in MSC, including aurora B kinase, FGF-2, cycline A2, eukaryotic initiation factor 4 E, and stromal cell-derived factor-1α. NPY directly induced neonatal and adult cardiomyocyte cell-cycle reentry and enhanced the number of differentiated cardiomyocytes from MSC in the infarcted myocardium, which corresponded to improved cardiac function, reduced fibrosis, ventricular remodeling, and increased angiomyogenesis. It is concluded that a combined treatment of NPY with MSC is a novel approach for cardiac repair.
Objective To investigate the expression of BRAF-activated long non-coding RNA (BANCR) in colorectal cancer,and the influence of BANCR on the biological function of HCT116 cells.Methods Fifty-six samples of colorectal cancer specimen (including the cancer tissues and precancerous tissues) were obtained at the First Affiliated Hospital of Nanjing Medical University from March 2012 to June 2013.The expressions of BANCR in all the specimens were detected by qRT-PCR (28 cases in the BANCR-high expression group and 28 cases in the BANCR-low expression group).The relationship between the expressions of BANCR and the clinicopathological factors of colorectal cancer was analyzed.The HCT116 cells were divided into 4 groups after interfering BANCR with lentiviral-mediated shRNA-1 and shRNA-2:interference group 1 (HCT116 cells transfected with LV-shRNA-1),interference group 2 (HCT116 cells transfected with LV-shRNA-2),negative control group (HCT116 cells transfected with lentivirus vector with nonsense sequence) and blank control group (HCT116 cells cultured in RPMI 1640 medium).The proliferation,apoptosis and migration of HCT116 cells in the 4 groups were detected by CCK-8 assay,flow cytometry and Transwell assay,respectively.The comparison between the 2 groups was analyzed by u test,and multiple groups were compared by one-way analysis of variance,repeated measurement analysis of variance and LSD-t test.Multivariate analysis was done by Logistic regression model.The difference between categorical data was compared by chi-square test.Results The relative expression of BANCR in the cancer tissues was 1.6 ± 0.4,which was significantly higher than 0.9 ± 0.7 of the precancerous tissues (u =1 020.000,P ＜ 0.05).The result of univariate analysis showed that the high expression of BANCR was correlated with the lymph node metastasis and tumor stage (x2 =4.595,7.487,P ＜ 0.05).The result of multivariate analysis showed that lymph node metastasis and tumor stage (stage Ⅲ-Ⅳ) were the independent risk factors influencing the high expression of BANCR(OR =4.000,5.914,95％ CI:1.230-12.900,1.685-20.760,P ＜ 0.05).The relative expressions of BANCR of the interference group 1,interference group 2,negative control group and the blank control group were 0.25 ±0.04,0.20±0.06,0.96 ±0.04,0.98 ±0.03,with significant difference among the 4 groups (F =271.610,P ＜ 0.05).The cell proliferation rates at day 6 of the interference group 1,interference group 2 and the negative control group were 80.6％ ± 7.6％,81.2％ ± 5.1％ and 87.9％ ± 13.6％,with no significant difference among the 3 groups (F =0.559,P ＞ 0.05).The apoptotic rates of the interference group 1,interference group 2,negative control group and the blank control group were 4.7％ ± 1.7％,5.1％ ± 1.1％,3.1％ ± 0.6％ and 2.8％ ± 0.9％,with no significant difference among the 4 groups (F =2.881,P ＞ 0.05).The numbers of transmembrane cells of the interference group 1,interference group 2,negative control group and the blank control group were 135 ± 29,107 ± 18,240 ± 24 and 245 ± 22,with significant difference among the 4 groups (F =45.194,P ＜ 0.05).Conclusions BANCR was overexpressed in the HCT116 cells,and the BANCR overexpression was correlated with lymph node metastasis and tumor stage.BANCR can promote the migration of HCT116 cells.BANCR could be an important biomarker for the diagnosis and prognosis of colorectal cancer.
Key words:
Colonic neoplasms; Rectal neoplasms; Long non-coding RNA; HCT116 cells; Migration
To cultivate the spirit and ability spirit of innovation for college students is the teaching goal,while the mechanical creative design competition is the best way that college students learn speculative knowledge for practise.The combination of mechanical creative design competition,in the teaching,optimiz
Twelve pulse shifted parallel connection thyristor rectifier were introduced, including the principle of major circuit and control circuit, the construction features and operation status.
Abstract Periodontitis, one of the most common chronic inflammatory diseases, affects the quality of life. Osteogenesis makes an important role of the disease. There is a connection between hydrogen sulfide (H 2 S) and periodontitis, but according to the study has been published, the precise role of H 2 S in inflammation remains in doubt. The main reason of the lack of research is that H 2 S is an endogenous gasotransmitter, difficult to discern through testing. So, we synthesis a novel fluorescence probe which can detective H 2 S in vitro. By using the novel H 2 S fluorescence probe, we found that H 2 S changes in osteoblasts mainly by cystathionine-γ-lyase, and H 2 S increases under LPS stimulation. H 2 S could be a potential marker for diagnosis of inflammatory diseases of bone, and might help deeper studies of the changes of H 2 S level and promote the progression on the researches about pathogenesis of periodontitis.
Recent research has highlighted the versatile functions of long non-coding RNAs (lncRNAs) in the onset and progression of various malignancies. Still, insufficient knowledge is available on how lnc-SOX9-4 functions in colorectal cancer (CRC) progression.Bioinformatics analysis was used to identify a novel lncRNA (lnc-SOX9-4), and the expression pattern of the RNA in CRC was verified using qRT-PCR. Gene ontology (GO) term analysis and Gene set enrichment analysis (GSEA) were implemented for the identification of the related mechanisms and roles of lnc-SOX9-4. Immune infiltration analysis was conducted for assessment of how lnc-SOX9-4 is linked to tumor immune cell infiltration level. Both in vitro and in vivo phenotype analyses were conducted for scrutinizing how lnc-SOX9-4 impacts the proliferation and metastasis of CRC. RNA pulldown, mass spectrometry analysis, fluorescent in situ hybridization (FISH), western blotting, and RIP assay aided in verifying lnc-SOX9-4 mechanisms linked to CRC progression.An upregulation of lnc-SOX9-4 was observed in the sample CRC cells and tissues. Elevated lnc-SOX9-4 levels showed a positive association with poor clinical prognosis. Lnc-SOX9-4 was closely correlated to several types of immune infiltrating cells. Functionally, the knockdown of lnc-SOX9-4 significantly inhibited CRC cell proliferation, migration, and invasion abilities. Mechanistically, YBX1 was identified as lnc-SOX9-4, specifically interacting protein in the nucleus. Lnc-SOX9-4 could stabilize YBX1 protein levels by inhibiting poly-ubiquitination and degradation of YBX1. Furthermore, phenotype rescue experiments reveal that lnc-SOX9-4 enhanced the CRC cellular potential to proliferate and metastasize by regulating YBX1 levels.Lnc-SOX9-4 promoted CRC progression by suppressing cytoplasmic translocation and promoting protein levels of YBX1 can serve as novel treatment targets for diagnosing and treating CRC.
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