Wei‐Ming Wang

National Defense Medical Center

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Chien‐Ping Chiang

Tri-Service General Hospital

Chih‐Tsung Hung

National Defense Medical Center

Yi‐Hsien Chen

Washington University in St. Louis

Hong‐Wei Gao

Lanzhou University Second Hospital

Chi‐Hsiang Chung

Taiwan Nurses Association

Ke‐Long Zou

Southern Medical University

Wu‐Chien Chien

National Defense Medical Center

Chang‐Huei Tsao

Tri-Service General Hospital

Yuyue Zhao

Southern Medical University

Shuting Liu

National Defense Medical Center

Cooperative Institutions

National Defense Medical Center

102

Shanghai Jiao Tong University

Tri-Service General Hospital

Wenzhou Medical University

Chinese Academy of Sciences

Central South University

Nanjing Medical University

Wuhan University

Chinese Academy of Medical Sciences & Peking Union Medical College

Ruijin Hospital

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The cytotoxic mechanism of epigallocatechin gallate on proliferative HaCaT keratinocytes

Journal of Biomedical Science (2017)

Yu-Wen Chu Shuting Liu Yalan Yang Shih‐Ming Huang Wei‐Ming Wang

Epigallocatechin gallate (EGCG) is the major ingredient of sinecatechins ointment, approved for the treatment of external genital and perianal warts. However, the molecular mechanism for EGCG's effect on warts resulting from the human papillomavirus (HPV) infection of keratinocytes is not well understood. HPV may survive in proliferative keratinocytes and may be involved in cell cycle regulation and progression. The objective of this study was to investigate the mechanism underlying EGCG's treatment on external genital warts of HPV infection through the cultured keratinocyte cells from the HaCaT cell line.MTT and flow cytometry assays were used to measure cell viability and the cell cycle profile, with and without EGCG treatment, for HaCaT keratinocyte cells cultured in a calcium-free medium and 1.8 mM calcium which induced proliferative and differentiated keratinocytes, respectively, for 24 h. The expression levels of cytotoxic proteins and factors were evaluated with the RT-PCR and western blotting analysis.EGCG influenced the proliferation stage but not the differentiation stage of keratinocytes. We suggest that apoptosis and autophagy might be the possible mechanism for the EGCG's effect on the proliferative HaCaT cells. Furthermore, we found that EGCG reduced the protein levels of cyclin D1 and Zac1 (a zinc-finger protein which regulates apoptosis and cell cycle arrest 1) dose-dependently in proliferative as compared to differentiated keratinocytes. It also induced the expression of p21 and DEC1 (differentiated embryo-chondrocyte expressed gene 1), and promoted G1 arrest of cell cycle in proliferative keratinocytes.These results help clarify the mechanisms of EGCG treatment of HPV-infected keratinocytes and may contribute to new targets, such as Zac1 and DEC1 for external genital and perianal warts.

HaCaT

Viability assay

MTT assay

10.1186/s12929-017-0363-7

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Citations (9)

Hormone replacement therapy did not increase risk of melanoma in Chinese female with menopausal and postmenopausal disorders: A population‐based retrospective cohort study in Taiwan

The Journal of Dermatology (2023)

Jun Ma Yi‐Hsien Chen Chien‐Ping Chiang Wei‐Ming Wang Chi‐Hsiang Chung

Hormone replacement therapy (HRT) is widely used to relieve symptoms of menopause with proven efficacy. However, there has been significant controversy surrounding the use of HRT because of its potential link with an increased risk of cancer, particularly female reproductive organ cancers. That HRT increases the risk of melanoma is also disputed, and several cohort studies have produced variable results. To delineate the association between HRT and melanoma in Taiwan, we conducted a population-based retrospective cohort study on 14 291 patients who had received HRT and 57 164 population controls in Taiwan between 2000 and 2013. Multivariate odds ratios (ORs) were calculated utilizing conditional logistic regression. Overall, the use of HRT was not significantly correlated with a higher risk of developing melanoma in Taiwan (95% confidence interval 0.386-1.099; p = 0.341). The hazard ratio analysis of melanoma and different HRTs showed there was no significant association between melanoma and the use of oral or external estrogens alone, including conjugated estrogens, estradiol, and estriol. Estrogen plus progesterone combined therapy was associated with a lower risk of melanoma. Only one case of melanoma was observed among the 2880 patients in this subgroup.

10.1111/1346-8138.16859

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Cover image

Journal of Oral Pathology and Medicine (2020)

Anjie Min Haofeng Xiong Wei‐Ming Wang Xin Hu Can Wang

The cover image is based on the Original Article CD147 promotes proliferation and migration of oral cancer cells by inhibiting junctions between E-cadherin and β-catenin by Anjie Min et al., https://doi.org/10.1111/jop.13088.

10.1111/jop.13107

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Wells’ syndrome associated with Churg-Strauss syndrome: correlation with mast cell distribution

International Journal of Dermatology (2012)

Ching‐Fu Huang Yufei Chen Wei‐Ming Wang Chien‐Ping Chiang

General hospital

10.1111/j.1365-4632.2011.05159.x

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Citations (4)

High throughput in vitro production of somatic embryoswith innovative cryo cell banking system

Horticulture International Journal (2022)

Wei‐Ming Wang La Shaun Sanders Alice Perry Ebonee Duncan

ArborGen Inc. was originally created as a partnership for forestry research in 2000 by West Rock (MWV), International Paper and Rubicon (Fletcher Challenge). It now produces the largest number of both conventional and advanced genetic tree seedlings in the world. Among its advanced genetics offerings, varietals have the highest level of genetic traits in yield and timber quality such as height, diameter growth, disease resistance and etc. To scale up varietals, in vitro process named somatic embryogenesis (SE) is used as a mean to mass propagate SE seedlings. Over the years of R&D efforts, we recognized that a key challenge for scale-up is the lack of a stable and predictable bioprocess platform. The primary causes that impact stability and predictability include a challenge to receive consistent supplies of good starting cell materials, the risk of somaclonal variation due to long term exposure tomaintenance media containing hormones and risk of contamination or loss from environmental fluctuations. Accordingly our SE R&D program has been targeted to develop a robust bioprocess platform to reduce these variabilities. One of our major achievement is the development of a cell banking (CB) system, which provides high quality starting cell materials for SE production. The cell banking system includes the creations of research cell banks (RCB) and master cell banks (MCB). After the cell banks are created, the banked cells are tested and validated for their qualification for embryo production. Currently, we successfully integrated the cell banking technology into somatic embryo production in a number of economically important tree species and crops, such as pine, spruce, sweetgum, sugarcane, avocado and banana. With the SE&CB platform, the somatic embryo & somatic seedling yields are consistent, predictable and are produced cost-efficiently on a commercial scale.

10.15406/hij.2022.06.00259

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Citations (1)

Nomogram for predicting cancer-specific survival of patients with buccal squamous cell carcinoma: a population-based study

Research Square (Research Square) (2020)

Wei‐Ming Wang xiuhong chu Sisi Yang Ying Wang chuanyu hu

Abstract Background The use of the traditional American Joint Committee on Cancer (AJCC) staging system alone has limitations in predicting the survival of buccal squamous cell carcinoma (BSCC) patients. We aimed to establish a comprehensive prognostic nomogram whose prognostic value compared with AJCC system. Methods Patients were obtained from the SEER (Surveillance, Epidemiology, and End Result) database. The Cox regression model was used to select variables using a backward stepwise selection method. Based on the predictive model for determining prognostic factors, a nomogram was developed to predict the cancer-specific survival rates of BSCC patients at 3, 5 and 8 years. We used several standard model validation methods to evaluate the performance of the survival model: concordance index (C-index), area under the time-dependent receiver operating characteristic curve (AUC), net reclassification improvement (NRI), calibration plots, decision curve analyses (DCAs), and integrated discrimination improvement (IDI). Results Multivariate analysis showed that age, AJCC stage, N stage, M stage, pathological grade, and surgical status were risk factors for survival. The c-index chart, the AUC chart, and the calibration chart show that the performance of the nomogram is better, and the NRI and IDI values show that the performance of the nomogram is better than that of the AJCC staging system. The 3-year, 5-year and 8-year DCA curves of nomogram show that it has more net benefit than traditional AJCC staging system, both in terms of training and validation queues. Conclusion We developed and validated the first BSCC prognosis nomogram, which has a better prognostic value than the separate AJCC staging system. Our BSCC prognosis nomogram is a valuable tool for explaining the risks of the next 3, 5 and 8 years in clinical practice.

Nomogram

AJCC staging system

10.21203/rs.3.rs-23785/v1

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Pregnancy outcomes in women with vitiligo: A Taiwanese nationwide cohort study

PLoS ONE (2021)

Chih‐Tsung Hung Hsin-Hui Huang Chun-Kai Wang Chi‐Hsiang Chung Chang‐Huei Tsao

Vitiligo is perceived as an autoimmune skin disease. Previous studies showed conflicting data about vitiligo and pregnancy outcomes. To delineate the associations between vitiligo and the pregnancy outcomes, we used the National Health Insurance Research Database of Taiwan to conduct a retrospective cohort study from January 1, 2000 to December 31, 2015. This study population was composed of 1,096 women with vitiligo and 4,384 women without vitiligo, who were all matched according to age, comorbidity, and index year. Compared with the non-vitiligo controls, women with vitiligo had a higher risk of abortion (aHR 1.158, 95% confidence interval (CI) 1.095–1.258, P < .001). Perinatal events, such as preterm delivery, pre-eclampsia/eclampsia, gestational diabetes mellitus, stillbirth, and intrauterine growth retardation, were not different between both groups (aHR 1.065, 95% CI 0.817–1.157, P = .413). To determine if systemic treatment before conception decreases the risk of abortion, we assessed the medical history of pregnant women with vitiligo 1 year before pregnancy. Patients who were treated with oral medications had a lower risk of abortion than those who were not (aHR: 0.675, 95% CI: 0.482–0.809, P < .001). Our study indicates that there is a higher risk of abortion in pregnant women with vitiligo and the control of disease activity with systemic treatment before conception could improve pregnancy outcomes.

Vitiligo

10.1371/journal.pone.0248651

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Insertion of Pro-Hyp-Gly provides 2 kcal mol⁻¹ stability but attenuates the specific assembly of ABC heterotrimeric collagen triple helices

Organic & Biomolecular Chemistry (2021)

Wei‐Ming Wang Chen‐Hsu Yu Jing‐Yuan Chang Ting‐Hsuan Chen Yan-Chen Chen

Collagen triple helices are stabilized by 2 kcal mol⁻¹ upon inserting proline-hydroxyproline-glycine but attenuates the specific assembly of the ABC heterotrimer.

Hydroxyproline

Collagen helix

10.1039/d0ob02190c

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Primary localized cutaneous nodular amyloidosis on areola

Indian Journal of Dermatology Venereology and Leprology (2022)

Shengwen Liu Chih‐Tsung Hung Hong‐Wei Gao Chien‐Ping Chiang Chih‐Yu Chen

Areola

Primary (astronomy)

10.25259/ijdvl_491_2021

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Genistein Up-Regulates the Expression of EGF and E-Cadherin in the Treatment of Senile Vaginitis

Molecules (2022)

Yarui Sun Lei Wang Bo Wang Yanli Meng Wei‐Ming Wang

Investigating the therapeutic effect of genistein (Gen) on postmenopausal senile vaginitis (SV) and its mechanism of action. Adult SPF female Wistar rats were selected to establish a bilateral ovariectomized animal model (OVX), which simulated senile vaginitis dominated by estrogen deficiency in ovarian dysfunction. After 14 days of continuous treatment, the morphology of vaginal epithelial tissue was observed and various types of epithelial cells were counted, and the body mass and uterine and vaginal index of rats were measured. the levels of vaginal tissue secretion, microorganism, hormone and glycogen in each group were measured and the reproductive health was evaluated clinically. The protein expression and mRNA expression of epidermal growth factor (EGF) and E-cadherin (E-cadherin) in vaginal tissues were detected by immunohistochemistry and RT-PCR, respectively. Result showed that Genistein lowered vaginal pH, increased vaginal index and vaginal health score, thickened epithelial layers and improved vaginal tissue atrophy after administration. Genistein also increased the contents of glycogen and Lactobacillus in vagina, and promoted the expression of EGF, E-cadherin protein and mRNA. To sum up, there is no significant change in serum E2 and FSH levels, indicating that genistein has no effect on hormone levels in rats. genistein promoted the proliferation of vaginal epithelial cells, thickened epithelial layers and the vaginal wall, which improved the resistance of vaginal epithelium, the recovery of self-cleaning ability and healed the vaginal wound and erosive surface to improve atrophy.

Vaginal atrophy

Intravaginal administration

10.3390/molecules27082388

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Citations (3)