Nan Wang

Liaoning University of Traditional Chinese Medicine

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Langchong He

Xi'an Jiaotong University

Yongjing Zhang

University of Jinan

Jue Wang

Xi'an Jiaotong University

Delu Che

Second Affiliated Hospital of Xi'an Jiaotong University

Shiling Hu

Xi'an Jiaotong University

Tao Zhang

Zhongshan Hospital of Xiamen University

Rui Liu

Zhejiang University

Yingnan Zeng

Jilin Agricultural Science and Technology University

Yanmin Zhang

First Affiliated Hospital of Xi'an Jiaotong University

Jianzhong Wang

Jilin Agricultural University

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Xi'an Jiaotong University

124

Chinese Academy of Sciences

Chinese Academy of Medical Sciences & Peking Union Medical College

Jilin Agricultural University

Huazhong University of Science and Technology

Peking University

Sichuan University

Shanghai Jiao Tong University

Nanjing Medical University

Capital Medical University

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Harpagoside-induced anaphylactic reaction in an IgE-independent manner both in vitro and in vivo

Immunopharmacology and Immunotoxicology (2018)

Delu Che Jiao Cao Rui Liu Jue Wang Yajing Hou

Harpagoside (HAR) is an active component of Scrophularia ningpoensis (SN), which has anti-inflammatory and anti-immune effects. SN is used widely in China to treat various diseases. Recently, SN has been used as a traditional Chinese medicine injection and used clinically. However, allergic responses to these injections are frequently reported.We examined whether the main component of SN, HAR, is associated with the allergic reaction to SN.This study assessed the effects of HAR in mice and mast cell activation to characterize its anaphylactic effects and underlying mechanisms. Mice hindpaw swelling, serum allergy factor detection, enzyme-linked immunosorbent assays, and degranulation assays were performed to measure allergic mediators both in vivo and in vitro.The present study indicated that HAR induced paw swelling, interleukin-6, inositol triphosphate, tumor necrosis factor-α, and histamine increases in mice. Our in vitro data also showed that HAR induced β-hexosaminidase, inositol triphosphate, and interleukin-6 release, leading to mast cell degranulation. In contrast, neither C48/80 nor HAR induced local anaphylaxis in STOCK KitW-sh/HNihrJaeBsmJNju mice.HAR is a potential sensitization compound in SN, and these results provide information for the safe clinical use of SN.

10.1080/08923973.2018.1424900

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Citations (3)

16s rDNA and qPCR of Con-SD mice

Figshare (2023)

Na Li Shuwen Tan Yue Wang Jiao Deng Nan Wang

Fecal samples were collected from control mice (Con) and 7-day sleep deprivation mice (SD).

10.6084/m9.figshare.22180414

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Citations (0)

Integration of network pharmacology, UHPLC-Q exactive orbitrap HRMS technique and metabolomics to elucidate the active ingredients and mechanisms of compound danshen pills in treating hypercholesterolemic rats

Journal of Ethnopharmacology (2024)

Shanlan Li Jin An Tong Zhang Guangyun Chen Zixuan Zhang

Pill

10.1016/j.jep.2024.118759

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Imidazolidinyl urea activates mast cells via MRGPRX2 to induce non-histaminergic allergy

Toxicology Research (2021)

Jiapan Gao Delu Che Xueshan Du Yi Zheng Huiling Jing

Imidazolidinyl urea (IU) is used as an antimicrobial preservative in cosmetic and pharmaceutical products. IU induces allergic contact dermatitis, however, the mechanism has not yet been elucidated. Mas-related G protein-coupled receptor-X2 (MRGPRX2) triggers drug-induced pseudo-allergic reactions. The aims of this study were to determine whether IU activated mast cells through MRGPRX2 to further trigger contact dermatitis. Wild-type (WT) and KitW-sh/HNihrJaeBsmJNju (MUT) mice were treated with IU to observe its effects on local inflammation and mast cells degranulation in vivo. Laboratory of allergic disease 2 cells were used to detect calcium mobilization and release of inflammatory mediators in vitro. WT mice showed a severe local inflammatory response and contact dermatitis, whereas only slight inflammatory infiltration was observed in MUT mice. Thus, MRGPRX2 mediated the IU-induced activation of mast cells. However, histamine, a typical allergen, was not involved in this process. Tryptase expressed by mast cells was the major non-histaminergic inflammatory mediator of contact dermatitis. IU induced anaphylactic reaction via MRGPRX2 and further triggering non-histaminergic contact dermatitis, which explained why antihistamines are clinically ineffective against some chronic dermatitis.

Histaminergic

10.1093/toxres/tfab035

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Laparoscopic distal gastrectomy and hyperthermic intraperitoneal chemotherapy in the treatment of advanced gastric cancer: a retrospective case-matched study on perioperative outcomes

Journal of Gastrointestinal Oncology (2021)

Zhiyuan Yin Mingguang Wei Shuang Xie Shuai Zhou Bo Zhang

This study aimed to assess the safety and efficacy of laparoscopic distal gastrectomy (LDG) with intraoperative hyperthermic intraperitoneal chemotherapy (HIPEC) for advanced gastric cancer (AGC).In this case-matched study, we retrospectively reviewed the database of 223 patients with AGC who underwent LDG in Tangdu Hospital from April 2016 to February 2019. Among all participants, 177 patients underwent LDG alone and 46 underwent LDG with HIPEC. We matched total of 138 (1:2) patients from the LDG + HIPEC group (n 46) and the LDG group (n 92) for gender, age, date of operation, and tumor-node-metastasis (TNM) stage of tumor.There was no significant difference in the Clavien-Dindo classification of complications between LDG alone and LDG + HIPEC patients. Further analysis showed the morbidity of gastroparesis to be significantly increased in LDG + HIPEC patients. At the same time, we found that the operation time, the time to 1st flatus, and hospital stay were longer in LDG + HIPEC patients and the incidence of abdominal recurrence 2 years after operation was significantly higher in the LDG group than the LDG + HIPEC group.The combination of LDG with intraoperative HIPEC is a safe and feasible method for AGC and HIPEC will limit the recovery of gastrointestinal functions. In addition, during the follow-up of our study, although there was no statistical difference between the two groups in abdominal recurrence at 2 years after surgery, a decreasing trend of abdominal recurrence in LDG + HIPEC patients could be seen in comparison to LDG patients.

Hyperthermic Intraperitoneal Chemotherapy

10.21037/jgo-21-20

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Citations (3)

Synthesis and characterization of authentic standards for the reductive-cleavage method. The positional isomers of partially methylated and acetylated or benzoylated 1,4-anhydro-l-fucitol

Carbohydrate Research (1995)

Nan Wang Larry E. Elvebak Gary R. Gray

Benzoates

Structural isomer

Derivative (finance)

Capillary gas chromatography

10.1016/0008-6215(95)00081-4

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Isoimperatorin reduces the effective dose of dexamethasone in a murine model of asthma by inhibiting mast cell activation

Phytotherapy Research (2020)

Jue Wang Yongjing Zhang Yingnan Zeng Shuai Ge Xiuzhen Sun

Adverse effects that result from dexamethasone (DEX) use are common and serious in patients with asthma. Therefore, alternative anti‐inflammatory treatments are being investigated. Isoimperatorin (ISO), an active natural furocoumarin, possesses multiple pharmacological properties, including an anti‐inflammation effect. In this study, investigations were conducted on the effect of ISO on mast cell (MC) activation in vitro and whether ISO could reduce the effective dose of DEX in a mast cell‐dependent murine model of asthma in vivo. Calcium imaging was used to assess intracellular Ca 2+ mobilization. Enzyme‐linked immunosorbent assay was used to measure the chemokines release. Western blot analysis was conducted to investigate the underlying pathway. Airway inflammation and hyperresponsiveness (AHR) were examined in an asthma model. ISO inhibited Ca 2+ flux and MC degranulation via Lyn/PLCγ1/PKC, ERK, and P38 MAPK pathways. In the asthma model, ISO, in combination with DEX, showed an additive inhibitory effect on AHR, inflammation, and the number of activated MCs in the lungs and decreased the levels of interleukin (IL)‐4, IL‐5, IL‐6, IL‐13, tumor necrosis factor (TNF)‐a, and C‐C motif chemokine ligand (CCL)‐2 in bronchoalveolar lavage fluid. A combination of DEX and ISO may be appropriate if a decrease in the steroid dose is desired owing to dose‐dependent adverse effects.

10.1002/ptr.6726

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Citations (8)

Mast cell-mediated hypersensitivity to fluoroquinolone is MRGPRX2 dependent

International Immunopharmacology (2019)

Rui Liu Shiling Hu Yongjing Zhang Delu Che Jiao Cao

10.1016/j.intimp.2019.02.001

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Citations (45)

Oral vaccination with a recombinant Lactobacillus plantarum expressing the Eimeria tenella rhoptry neck 2 protein elicits protective immunity in broiler chickens infected with Eimeria tenella

Parasites & Vectors (2024)

Tongxuan Zhang Hangfan Qu Wei Zheng Yanan Zhang Yanning Li

Chicken coccidiosis is a protozoan disease that leads to considerable economic losses in the poultry industry. Live oocyst vaccination is currently the most effective measure for the prevention of coccidiosis. However, it provides limited protection with several drawbacks, such as poor immunological protection and potential reversion to virulence. Therefore, the development of effective and safe vaccines against chicken coccidiosis is still urgently needed.

Parasitology

Mucosal Immunity

10.1186/s13071-024-06355-w

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Citations (4)

[Nutritional status alters the mRNA expressions of galanin and its receptors in taste buds of rats].

PubMed (2019)

Ke Chen Jianqun Yan Bo Sun Xiao Luo Nan Wang

The nutritional and metabolic status alters the peripheral taste perception and food intake by participating in the modulation of taste information integration. The taste receptors and neuropeptides in the taste buds are the important targets of this modulation process. To explore the effects of nutritional status on the expressions of galanin and its receptors in the taste buds, we compared the mRNA levels of galanin and its specific receptor GalR2 in the taste buds among the high-fat diet induced obese rats (HF), chronically restricted diet rats (CR) and control rats. The high-fat diet, half of chow diet, and normal chow diet were given to HF, CR and control groups for 6 weeks, respectively. The body weight and some metabolic indexes, including blood glucose, triglyceride and cholesterol levels were detected. The mRNA expressions of galanin and its receptors in taste buds were determined using real-time PCR. Results showed that compared with control rats, the body weights, levels of blood glucose and triglyceride were significantly elevated in HF rats; while the mRNA expressions of galanin and GalR2 were dramatically decreased. However, galanin mRNA expression in CR rats was increased to 2.3 times of that in control group. Considering the results obtained from our previous studies, we conclude that the behavioral changes in tasting choice of HF rats may be related to the expressions of galanin and GalR2 in the taste buds. The changes of galanin and GalR2 in taste buds are involved in the peripheral mechanism of nutritional status regulating taste perception and feeding behavior in rats.

Taste receptor

Taste bud

Umami

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Citations (1)