Huimin Chen

Qingdao University of Science and Technology

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Jing‐Yuan Fang

Shanghai Jiao Tong University

Guangmin Xia

Shandong University

Yanwei Lin

Guangdong Medical College

Jia‐Yin Tang

Renji Hospital

Zhi‐Zheng Ge

Renji Hospital

TaChung Yu

Renji Hospital

Haoyan Chen

Renji Hospital

Jinxian Chen

Renji Hospital

Xianglong Tian

Shanghai Sixth People's Hospital

Zheng Wang

Shanghai Jiao Tong University

Cooperative Institutions

Shanghai Jiao Tong University

Chinese Academy of Sciences

Renji Hospital

Sun Yat-sen University

Huazhong University of Science and Technology

Shandong First Medical University

Chinese Academy of Medical Sciences & Peking Union Medical College

Shandong University

Central South University

Nanjing Medical University

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Supplementary Tables 1-6 from RING-Finger Protein 6 Amplification Activates JAK/STAT3 Pathway by Modifying SHP-1 Ubiquitylation and Associates with Poor Outcome in Colorectal Cancer

Qian Liang Dan Ma Xiaoqiang Zhu Zhenhua Wang Tiantian Sun

Table S1.Expression of human ubiquitin ligase genes in 246 CRC patients of TCGAdatabase.TableS2.Clinial information of 62 cases CRC patients (fresh tissues).Table S3.Clinial information of 78 cases CRC patients (paraffin tissues).Table S4.Clinial information of 97 cases CRC patients (paraffin tissues).Table S5.RNA sequence data. TableS 6. Primer sequences

Primer (cosmetics)

Table (database)

10.1158/1078-0432.22461027

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Plant Regeneration from Cell Supension Derived Protoplasts of Heracleum moellendorffii

Journal of Integrative Plant Biology (1993)

Guangmin Xia Zhongyi Li Huimin Chen

Protoplast

Source

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Somatic embryogenesis and plant regeneration from protoplasts isolated from embryogenic cell suspensions of wheat (Triticum aestivum L.)

Plant Cell Tissue and Organ Culture (PCTOC) (1992)

Zhongyi Li Guangmin Xia Huimin Chen

Protoplast

Suspension culture

10.1007/bf00039918

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Citations (36)

Synthesis and characterization of N-fumaroylated diketopiperazine for pulmonary drug delivery

Materials Express (2023)

Na Liu Wěi Li Huimin Chen Hao Wang

In this study, we developed (2E,2′E)-4,4′-((((2S,5S)-3,6-dioxopiperazine-2,5-diyl)bis(butane-4,1-diyl))bis(azanediyl))bis(4-oxobut-2-enoic acid) (FDKP), which is a new pulmonary inhalation excipient approved by the FDA in recent years. FDKP is an excellent drug carrier excipient, it shows different adsorption and release characteristics to various positively charged peptides, proteins and other drugs in different acidic environments, and finally the FDKP can distribute to other tissues and excreted from the body through the kidney in its prototype. In previous studies, the yield of FDKP was very low, the raw materials were difficult to obtain and the cost was too high. In this study, we optimized the reaction conditions and synthetic route, by changing inconvenient and expensive starting materials to easily available cheap materials, through five steps including dehydration cyclization, hydrogenation, condensation, saponification, and recrystallization, we finally obtained FDKP with 70% yield in total. And then we evaluated the cytotoxicity of FDKP in vitro through the MTT assay. It showed that FDKP synthesized by this method was safe. The viability of cells remains at high level (>80%) at the maximum concentration of 5 mg/ml for 24 h and also above 80% at 2.5 mg/ml for 48 h.

Saponification

Excipient

Butane

10.1166/mex.2023.2278

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Post‐transcriptional polyadenylation site cleavage maintains 3′‐end processing upon DNA damage

The EMBO Journal (2023)

Rym Sfaxi Biswendu Biswas Galina Boldina Mandy Cadix Nicolas Servant

The recognition of polyadenylation signals (PAS) in eukaryotic pre-mRNAs is usually coupled to transcription termination, occurring while pre-mRNA is chromatin-bound. However, for some pre-mRNAs, this 3'-end processing occurs post-transcriptionally, i.e., through a co-transcriptional cleavage (CoTC) event downstream of the PAS, leading to chromatin release and subsequent PAS cleavage in the nucleoplasm. While DNA-damaging agents trigger the shutdown of co-transcriptional chromatin-associated 3'-end processing, specific compensatory mechanisms exist to ensure efficient 3'-end processing for certain pre-mRNAs, including those that encode proteins involved in the DNA damage response, such as the tumor suppressor p53. We show that cleavage at the p53 polyadenylation site occurs in part post-transcriptionally following a co-transcriptional cleavage event. Cells with an engineered deletion of the p53 CoTC site exhibit impaired p53 3'-end processing, decreased mRNA and protein levels of p53 and its transcriptional target p21, and altered cell cycle progression upon UV-induced DNA damage. Using a transcriptome-wide analysis of PAS cleavage, we identify additional pre-mRNAs whose PAS cleavage is maintained in response to UV irradiation and occurring post-transcriptionally. These findings indicate that CoTC-type cleavage of pre-mRNAs, followed by PAS cleavage in the nucleoplasm, allows certain pre-mRNAs to escape 3'-end processing inhibition in response to UV-induced DNA damage.

Cleavage (geology)

Cleavage stimulation factor

Directionality

10.15252/embj.2022112358

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Citations (4)

Zuogui Wan alleviated maternal kidney-yin deficiency-induced thymic epithelial cell dysfunction in newborn rats through Wnt/β-catenin signaling pathway

Journal of Ethnopharmacology (2021)

Longyun Chen Meirong Ren Jigang Cao Hongling Sang Huimin Chen

WNT4

Viability assay

10.1016/j.jep.2021.114337

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Carbapenem antibiotic stress increases bla_KPC_‐2 gene relative copy number and bacterial resistance levels of Klebsiella pneumoniae

Journal of Clinical Laboratory Analysis (2022)

Huimin Chen Na Li Fang Wang Lei Wang Wei Liang

The clinical isolation rates of carbapenem-resistant Klebsiella pneumoniae (CR-KP) continue to increase. In China, clinical CR-KP isolates are mainly attributed to the blaKPC-2 gene carried on plasmids, and the blaKPC-2 copy number correlates with the expression of KPC enzymes, which can cause elevated carbapenem MICs.Thirty-seven CR-KP isolates were collected at the Second People's Hospital of Lianyungang City between January 2020 and March 2021, with no duplicate isolates, and were screened for the blaKPC-2 gene with PCR. Analysis of current CRKP resistance to clinically relevant antimicrobials using the bioMérieux VITEK® 2 bacterial identification card. The multilocus sequence types of the strains were confirmed with PCR and DNA sequencing. Recombinant plasmids pET20b-blaKPC-2 and pET20b-CpsG were constructed, and the copy numbers of the recombinant plasmids per unit volume was calculated based on the molecular weight of the plasmids. After the genomes DNA of clinical isolates of K. pneumoniae carrying the blaKPC-2 gene were purified, the blaKPC-2 gene relative copy number in individual K. pneumoniae strains was indicated by the double standard curve method. Detection of MIC values changes of K. pneumoniae under imipenem selection pressure by broth microdilution method.Among the 37 CR-KP strains isolated, only the blaKPC-2 gene was detected in 30 strains, three strains were positive for the blaNDM-1 gene, two strains carried both the blaKPC-2 and blaNDM-1 genes, and two strains without detectable carbapenem resistance genes. The ST11 clone was predominant among the 37 carbapenem-resistant K. pneumoniae isolates. Drug sensitivity testing showed that except for polymyxins (100% susceptible) and tigecycline (75.7% intermediate), the 37 CR-KP strains were resistant to almost all antimicrobial drugs. The blaKPC-2 relative copy number in nine ST11 clinical isolates of K. pneumoniae was 7.64 ± 2.51 when grown on LB plates but 27.67 ± 13.04 when grown on LB plates containing imipenem. Among these nine isolates, five CRKP strains exhibited elevated MICs to imipenem, while the remaining four strains showed unchanged MIC values to imipenem.Carbapenem-resistant Klebsiella pneumoniae isolates may have multiple pathways to achieve high levels of carbapenem resistance, and moderate carbapenem pressure can increase the copy number of KPC enzyme genes in CRKP strains and enhance the degree of carbapenem resistance in the strains.

Carbapenem

Broth microdilution

10.1002/jcla.24519

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Citations (3)

Integrated transcriptomics and metabolomics analyses provide insights into anthocyanin biosynthesis for leaf colour formation in Quercus mongolica

Research Square (Research Square) (2024)

Yangchen Yuan Jialin Liu Xinman Li Zipeng Zhao Jiushuai Pang

Abstract Quercus mongolica is a tall tree with a broad, rounded crown and lush leaves. In autumn, the leaves turn red and have great ornamental value. However, the molecular mechanisms that cause the change in leaf colour are unknown. In this study, we identified 12 differentially expressed genes involved in anthocyanin synthesis by analysing the transcriptome of Q. mongolica leaves in six developmental stages (S1 − S6). We further analysed the dynamics of anthocyanin content in Q. mongolica leaves in four developmental stages (S1, S2, S5, and S6) using differential gene expression patterns. We detected a total of 48 anthocyanins and categorised these into seven major anthocyanin ligands. The most abundant anthocyanins in the red leaves of Q. mongolica were cyanidin-3,5-O-diglucoside, cyanidin-3-O-glucoside, cyanidin-3-O-sophoroside, and pelargonidin-3-O-glucoside. Correlation analysis of differentially expressed genes and anthocyanin content identified highly expressed QmANS as a key structural gene associated with anthocyanin biosynthesis in Q. mongolica . A transcription factor-structural gene correlation analysis showed that the 1bHLH, 3bZIP, 1MYB, 10NAC, and 2WRKY transcription factors played strong positive roles in regulating anthocyanin structural genes (|PCC| > 0.90), with the QmNAC transcription factor playing a major role in anthocyanin biosynthesis.

Pelargonidin

Structural gene

10.21203/rs.3.rs-3845207/v1

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Data from RING-Finger Protein 6 Amplification Activates JAK/STAT3 Pathway by Modifying SHP-1 Ubiquitylation and Associates with Poor Outcome in Colorectal Cancer

Qian Liang Dan Ma Xiaoqiang Zhu Zhenhua Wang Tiantian Sun

<div>AbstractObjective: The E3 ubiquitin ligase RNF6 (RING-finger protein 6) plays a crucial role in carcinogenesis. However, the copy number and expression of RNF6 were rarely reported in colorectal cancer. We aimed to explore the mechanical, biological, and clinical role of RNF6 in colorectal cancer initiation and progression.Design: The copy number and expression of RNF6 were analyzed from Tumorscape and The Cancer Genome Atlas (TCGA) datasets. Gene expressions were examined by real-time PCR, Western blot, and immunohistochemical staining. Gene expression profiling studies were performed to identify pivotal genes regulated by RNF6. Biological function of RNF6 on tumor growth and metastasis was detected in vivo and in vitro. Role of RNF6 in modulating SHP-1 expression was examined by coimmunoprecipitation and confocal microscopy, respectively.Results: The copy number of RNF6 was significantly amplified in colorectal cancer, and the amplification was associated with RNF6 expression level. Amplification and overexpression of RNF6 positively correlated with patients with colorectal cancer with poor prognosis. The gene set enrichment analysis (GSEA) revealed cell proliferation, and invasion-related genes were enriched in RNF6 high-expressed colorectal cancer cells as well as in patients from TCGA dataset. Downregulation of RNF6 impaired the colorectal cancer cell proliferation and invasion in vitro and in vivo. RNF6 may activate the JAK/STAT3 pathway and increase pSTAT3 levels by inducing the ubiquitination and degradation of SHP-1.Conclusions: Genomic amplification drives RNF6 overexpression in colorectal cancer. RNF6 may be a novel biomarker in colorectal carcinogenesis, and RNF6 may increase pSTAT3 level via promoting SHP-1 ubiquitylation and degradation. Targeting the RNF6/SHP-1/STAT3 axis provides a potential therapeutic option for RNF6-amplified tumors. Clin Cancer Res; 24(6); 1473–85. ©2017 AACR.</div>

10.1158/1078-0432.c.6524673

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Inhibition of the lncRNA 585189 Prevents Podocyte Injury and Mitochondria Dysfunction by Promoting hnRNP A1 and SIRT1 in Diabetic Nephropathy

Journal of the American Society of Nephrology (2023)

Jinxiu Hu Yue Liu Huimin Chen Zhimei Lv Rong Wang

Journal of the American Society of Nephrology 34(11S):p 498, November 2023. | DOI: 10.1681/ASN.20233411S1498a

10.1681/asn.20233411s1498a

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