Libin Wen

Ministry of Agriculture and Rural Affairs

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Kongwang He

Yangzhou University

Aihua Mao

Shantou University

Xuehan Zhang

Peking Union Medical College Hospital

Zhengyu Yu

Sichuan University

Yanxiu Ni

Jiangsu Academy of Agricultural Sciences

Lixin Lv

Tongji Hospital

Jianping Xie

Southwest University

Junming Zhou

Jiangsu Academy of Agricultural Sciences

Rongli Guo

Jiangsu Academy of Agricultural Sciences

Qi Xiao

Ministry of Agriculture and Rural Affairs

Cooperative Institutions

Jiangsu Academy of Agricultural Sciences

Nanjing Agricultural University

Yangzhou University

Ministry of Agriculture and Rural Affairs

Chinese Academy of Sciences

Chinese Academy of Agricultural Sciences

China Agricultural University

University of Chinese Academy of Sciences

Zhejiang University

Tibet University

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Effects of Porcine Circovirus-like Agent P1 Infection on the Porcine Antiviral Protein mRNA Transcriptions in Peripheral Blood Mononuclear Cells of Piglets

Acta Agriculturae Boreali-Sinica (2010)

Libin Wen

The study was to explore the effects of porcine circovirus-like agent P1 infection on the porcine anti-viral protein mRNA transcriptions.About thirty-day-old healthy Guangxi Bama miniature pigs were inoculated with cloned genomic DNA of P1.The changes of mRNAs of porcine antiviral protein(PKR,2'-5'OAS2,RNase L and Mx1) in peripheral blood mononuclear cells(PBMC) of piglets were analyzed by real-time fluorescent quantitative PCR(real-time FQ-PCR) .The results showed that the transcriptions of PKR mRNAs were increased in the affected piglets at 8 and 40 day post-inoculation(DPI) ,respectively.Whereas 2'-5'OAS2 mRNA levels of the inoculated pigs were downregulated at 3 DPI and 17DPI,the P1 infected group trended to decrease expression of Mx1 mRNA at 17DPI as well.RNase L mRNA expressions appeared to be no significant change by P1 infection.In conclusion,the results indicated that P1 infection can affect the mRNA levels of PKR,2'-5'OAS2 and Mx1.

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Genetic Polymorphism of Ral Guanine Nucleotide Dissociation Stimulator in LLC-PK1 Cells

Jukun Song Na Li Xiaomin Dong Jianping Xie Hoi Kiong Lai

This study aimed to investigate the polymorphism of porcine-origin Ral guanine nucleotide dissociation stimulator (RalGDS) in LLC-PK1 cells using RT-PCR and sequencing. Our results revealed seven polymorphisms in the RalGDS gene, including insertions, deletions, and frameshift mutations. These variations may significantly alter the protein structure and function of RalGDS, potentially influencing its role in Ral GTPase-mediated signaling pathways. This work provides foundational insights into the genetic diversity of porcine RalGDS and its implications for pig physiology and economically important traits.

10.20944/preprints202502.1727.v1

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The Concept of Viewing the Situation as a Whole in the Theoretical System of Traditional Chinese Veterinary Medicine

Animal husbandry and feed science (2004)

Rui Wang Dan Cunfu Libin Wen

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Basigin-CyP elevated porcine circovirus type2 replication

Virus Research (2020)

Xuejiao Zhu Libin Wen Wei Wang Qi Xiao Kongwang He

Basigin

Circovirus

10.1016/j.virusres.2020.198152

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Genetic and phylogenetic analysis of a new porcine circovirus type 2 (PCV2) strain in China

Archives of Virology (2015)

Zhang Dan Kongwang He Libin Wen Hongjie Fan

Circovirus

Lineage (genetic)

10.1007/s00705-015-2615-8

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High-resolution Melting Analysis for Differentiating Porcine Circovirus-like Agent P1 and Porcine Circovirus Type 2

Jiangsu nongye xuebao (2010)

Libin Wen Kongwang He Yang Hanchun Guo RongLi Zhong ShuLin

To assess the use of high-resolution melting(HRM) analysis for rapid identification of porcine circovirus-like agent P1 and porcine circovirus type 2(PCV2),the targeted DNA sequence was amplified by Corbett Rotor-Gene 6000 real-time PCR cycler in the presence of the EvaGreen fluorescent dye.A working protocol of HRM analysis were established.Under the optimized assay conditions,P1 and PCV2 were amplified and two HRM types were differed by only 1 bp.This assay provides a new approach for P1 and PCV2 detection using a new molecular diagnostic method.

High Resolution Melt

Circovirus

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Sequencing and analysis of complete genome of a porcine circovirus type 2-like agent P1.

Zhongguo nongye Kexue (2010)

Libin Wen Kongwang He Yang Han-chun

【Objective】 The aim of the experiment is to discover an agent containing high homologous sequences of porcine circovirus type 2 in swine. 【Method】 PCR was developed for detection of DNA extracted from porcine sera collected from pigs with clinical signs of postweaning multisystemic wasting syndrome,then a reverse PCR was carried out based on the acquired sequences in this study,the sequences of PCR fragments were assembled into consecutive sequence and then finally verified by amplifing the agent complete genome. 【Result】 Complete nucleotide sequence of genome of porcine circovirus type 2-like agent P1 was firstly reported in this paper. P1 contained circular genomic DNAs of 648 nucleotides and three open reading frames. Except 16 nucleotides in the 5' end of the genome,the genome of P1 showed 98.42% of nucleotide homology compared with porcine circovirus type 2 BF isolate. Phylogenetic analysis revealed that P1 was closely related to the porcine circovirus. 【Conclusion】 Results suggested that there existed porcine circovirus type 2-like agent P1 in swine.

Circovirus

genomic DNA

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Induction of anti-EBNA-1 protein by 12-O-tetradecanoylphorbol-13-acetate treatment of human lymphoblastoid cells

Journal of Virology (1989)

Libin Wen Akiko Tanaka Meihan Nonoyama

Binding of the Epstein-Barr virus (EBV) nuclear antigen (EBNA-1) to BamHI-C DNA was studied by affinity column chromatography followed by immunoblotting with human serum specific for EBNA-1. Two species of EBNA-1 (68 and 70 kilodaltons) were identified in nuclear extracts of the EBV-positive Burkitt's lymphoma cell line Raji and not in nuclear extracts of the EBV-negative Burkitt's lymphoma cell line BJAB. Both EBNA-1s bound specifically to the region required for EBV plasmid DNA maintenance (oriP) located in the BamHI-C fragment. Upon treatment with 12-O-tetradecanoylphorbol-13-acetate, which activates latent EBV genome in Raji cells, the 68-kilodalton EBNA-1 was uncoupled from binding to EBV oriP. Nuclear extracts from 12-O-tetradecanoylphorbol-13-acetate-treated BJAB cells also uncoupled the binding of both EBNA-1s to oriP. DNA-cellulose column chromatography identified two protein species which competed for and uncoupled the binding of EBNA-1 to oriP. The two cellular competitors we called anti-EBNA-1 proteins had molecular masses of 60 and 40 kilodaltons, respectively. They were not found in nuclear extracts of BJAB cells not activated by 12-O-tetradecanoylphorbol-13-acetate.

12-O-Tetradecanoylphorbol-13-acetate

Raji cell

Lymphoblast

10.1128/jvi.63.8.3315-3322.1989

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Anti EBNA-1 Protein Induced with TPA

Humana Press eBooks (1989)

Libin Wen Akiko Tanaka Meihan Nonoyama

Binding of the EBNA-1 to ori-P was studied by affinity column chromatography followed by immunoblotting with anti EBNA-1 serum. Treatment of Raj i cells with phorbol ester, which activates the EBV replicative life cycle, induced the production of two cellular competitors which upon competition uncoupled the binding of EBNA-1 to ori-P. Gel retardation assay, DNase I footprinting and methylation interference analysis showed that the two cellular competitors bound specifically to the 20x 30 bp tandem repeat clusters in the EBV ori-P.

Footprinting

10.1007/978-1-4612-4508-7_15

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Detection of 12-o-Tetradecanoylphorbol-13-Acetate-induced Cellular Proteins That Compete with the Epstein-Barr Virus Nuclear Antigen 1 (EBNA-1) for Binding to a Site within the Epstein-Barr Virus oriP

Advances in experimental medicine and biology (1990)

Meihan Nonoyama Libin Wen Akiko Tanaka Guy Bradley

Virus latency

Transcription

Immortalised cell line

10.1007/978-1-4684-5853-4_13

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