Highly pathogenic coronaviruses have caused significant outbreaks in humans and animals, posing a serious threat to public health. The rapid global spread of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has resulted in millions of infections and deaths. However, the mechanisms through which coronaviruses evade a host’s antiviral immune system are not well understood. Liquid–liquid phase separation (LLPS) is a recently discovered mechanism that can selectively isolate cellular components to regulate biological processes, including host antiviral innate immune signal transduction pathways. This review focuses on the mechanism of coronavirus-induced LLPS and strategies for utilizing LLPS to evade the host antiviral innate immune response, along with potential antiviral therapeutic drugs and methods. It aims to provide a more comprehensive understanding and novel insights for researchers studying LLPS induced by pandemic viruses.
Cormorants, as protected wild animals by the State Forestry Administration of China, have a broad distribution across China. Previous studies have shown that they can be infected with multiple viruses in the Flaviviridae, Orthomyxoviridae, Paramyxoviridae, and Polyomaviridae families. There is limited knowledge about the other viruses that cormorants may carry and infect. In this study, we employed viral metagenomics to identify novel viruses in the fecal samples collected from cormorants in Xiamen City, Fujian Province, China. Two novel viruses were identified, including one novel picornavirus named Cormhepa01 and one novel avain hepevirus named CormhepaE. The genome of Cormhepa01 is 7,463 bp in length, which encodes a 2,260 aa polyprotien. Similar to other known picornaviruses, the conserved NTPase, proteinase, and polymerase motifs are presented in the 2C, 3C, and 3D proteins separately. Based on the phylogenetic analysis and amino acid sequence alignment, the CormhepaE may be assigned to a new picornavirus genus. The partial genome of CormhepaE is 6,546 bp in length. Compared with other avian hepatitis E virus strains, CormhepaE has multiple variable sites, which are distributed in motifs of the methyltransferase, helicase, and RdRp domains, separately. Based on the phylogenetic analysis, CormhepaE, together with another strain MG737712 isolated from sparrow, formed a new species of the Avihepevirus genus in the Hepeviridae family. We identified and characterized two novel cormorant viruses in this study. The findings of this study increase our understanding of the diversity of viruses in cormorants and provide practical viral genome information for the prevention and treatment of potential viral diseases affecting this species.
This paper shows how designers use Shape Grammar to create shape and form in tractable manner. The Shape Grammar was developed in an article by Georgy Stiny and James Gips in 1970. Using the approach of Shape Grammar can generate large design solution spaces and get a lot of possible results. The parametric shape grammar provides an effective way to extend design ideas in the process of transportation design.
Acknowledgments Financial support of this work was provided by the National Key Technology Support Program of the People’s Republic of China, under grant number 2013BAI03B06. This research work was jointly supported by the Joint Specialized Research Fund for the Doctoral Program of Higher Education of the Ministry of Eduction of the People’s Republic of China under grant number 20132307110018, and the Graduate Student Innovation Research Project in Harbin Medical University of the People’s Republic of China under grant number YJSCX2015-32HYD. View the original paper here.
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