Brown planthopper (BPH) is the most destructive insect pest to rice that causes tremendous yield loss each year in rice planting Asia and South-East Asia areas. Compared with traditional chemical-based treatment, utilization of plant endogenous resistance is a more effective and environmental-friendly way for BPH control. Accordingly, quite a few quantitative trait loci (QTLs) for BPH resistance were cloned using forward genetics. However, BPH is apt to change quickly into new biotypes to overcome plant resistance, therefore, new resistance resources and genes are continuously needed. miRNAs are important regulators in both plant development and physiological regulation including immunity, and might be used as effective supplements for BPH resistance QTLs. miR159 is an ancient and conserved miRNA. In this study, we found that each OsMIR159 gene in rice responded to BPH feeding very obviously, and genetic function assay proved them to negatively regulate BPH resistance, with STTM159 showing resistance to BPH, and over expression of OsmiR159d susceptible to BPH. One target genes of OsmiR159, OsGAMYBL2, positively regulated BPH resistance. Further biochemical studies revealed that OsGAMYBL2 could directly bind to the promoter of G-protein γ subunit encoding GS3 gene and repress its expression. And genetically, GS3 responded to BPH feeding promptly and negatively regulated BPH resistance, GS3 over expression plants were susceptible to BPH, while GS3 knock-out plants were resistant to BPH. Thus, we identified new function of OsmiR159-OsGAMYBL2 in mediating BPH response, and revealed a new OsmiR159-G protein pathway that mediates BPH resistance in rice.
In recent decades, a lot of quality and design problems of urban footbridge appear, these bridge problems often causes traffic jams and different traffic accidents, more serious bridge problem will cause certain people casualties.This paper uses universal finite element software ANSYS to establish three-dimensional finite element model of urban footbridge, and analyse and explain calculation results, and researchs stress and deformation distribution of urban footbridge under different cases.Research results can provide a certain reference basis for design and construction of the reinforced concrete urban footbridge.
After introduction of structure and types of the external floating hose for FPSO,the pressure tests were carried out to the four types(the first,the head,the tail and the side tube) of 16 inch floating hoses on the sea which were designed and manufactured domestically.The experimental results were compared with the international common OCIMF standards to verify their performance of the external floating hose for FPSO.
One Acyl-CoA binding protein(ACBP) encoding gene was isolated from testis of Onychostoma macrolepis using homologous cloning and the RACE method. The full Oma-ACBP cDNA(GenBank accession no: JN254628) was 503 bp long and comprised 37 bp of the 5'-Untranslated Regions(UTR), 267 bp of the coding sequence(CDS) encoding a 88 amino acid proteins, and 199 bp of the 3'- Untranslated Regions(UTR) with the polyA tail. A condensed phylogenetic tree show that Oma-ACBP had similarities with vasas of fish species and even mammal and amphibian species, The Oma-ACBP shared 92% sequenced identity with Cyprinus carpio and 91% with Carassius auratus, including a conserved FABP domain, the result enriches our understanding in the study of sequence classification in Onychostoma macrolepis. The Quantitative real-time PCR analysis demonstrated that Oma-ACBP was highly expressed in intestine, spleen and liver, but weakly in testis, ovary, heart, brain, cheek, muscle and eye. It was highly expressed in liver and spleen(42% of dietary protein), weakly expressed in intestine (52% of dietary protein). In starvation challenge, the expression was weakly expressed from 5 day to 8 day, and highly expressed from 3 day to 5 day, and the expression kept stabilizing until the end of the experiment.
Abstract With the continuous development of China’s general aviation industry, there are urgent requirements for research on aircraft crashworthiness to provide better safety for aircraft occupants. According to the results of long-term crash investigations, if crash safety design is taken into account at the earliest stages of aircraft design, most accidents will become viable or partially survivable, which can greatly improve the survival rate of aircraft passengers after an accident. In this paper, the design criteria and design methods for crash resistance technology of aircraft cargo uphold pillars are studied, and the crash simulation analysis of a certain type of aircraft is analyzed. First, a feasible design method and optimization principle are formulated according to the specific characteristics of a certain type of aircraft; Then, the crash resistance characteristics of a certain type of aircraft are analyzed, and the finite element model of the fuselage and cargo uphold pillars structure is established based on the three-dimensional electronic prototype of the aircraft. By the crash conditions specified in the relevant specifications, a mechanical simulation study of the aircraft is carried out, and the results are analyzed. Finally, constructive comments are given based on the simulation results. The reliability of the design is verified using simulation analysis, which provides a reference for the simulation and analysis of the anti-crash design of the aircraft cargo uphold pillars structure.
Abstract To compare the two methods for determining the genome size of Taimenes ( Hucho taimen ). The present study determined genome size (or C- values) of 30 individuals of Taimenes by the method of PI (propidium iodide) and DAPI (4’,6-diamidino-2-phenylindole), through the flow cytometry, using chicken erythrocyte DNA content as a standard. The result showed that (1) With the method of PI and DAPI through the flow cytometry, DNA content in blood erythrocyte was 5.65±0.25 pg•2C-1 and 5.04±0.12 pg•2C-1(Fig. 1 and Fig. 2), and genome size (Gb) were 2.70 ± 0.12 Gb and 2.41 ± 0.06 Gb. By Independent-Samples T Test, the genome size (Gb) of blood erythrocyte determined by PI was significantly greater than that determined by DAPI(Table 1). (2)Several of the main support salmonids were clustered by furthest neighbor of any two C-values with the measure of euclidean distance. From the Fig. 3, we found that taimen and fine scale ( Brachymystax lenok )first clustered into the same subgroup 1, brook trout ( Salvelinus fontinalis ) and Atlantic salmon ( Salmo salar ) clustered into the same sub-group 2, rainbow trout (Oncorhynchus mykiss) and pelyad ( Coregonus peled ) clustered into the same sub-group 3, Subgroup 1 and subgroup 2 firstly clustered into the same team, then come together with subgroup 3. The result indicates that (1) PI method can more accurately reflect the distribution of DNA content in blood erythrocyte of taimen, and more accurate prediction genome size of taimen.(2)Cluster analysis of C-values of several salmons may be related to their respective origin distribution and the filter feeding characteristics of Pelyad.
Abstract In this paper, the topology optimization algorithm is used to optimize the main structure of the main landing gear lower door of an aircraft by combining the structural strength, structural stiffness, and processing technology [1-2] . Considering the balance of structural weight, structural strength, and material distribution, the structural topology optimization design result with reasonable structure, good manufacturability, and moderate weight is finally obtained.
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