S Aleksić

Institut für Hygiene und Umwelt

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J Bockemühl

Institut für Hygiene und Umwelt

Verica Aleksić Sabo

University of Novi Sad

R Rohde

Texas State University

Helge Karch

Institut für Hygiene und Umwelt

Heinrich Neubauer

Friedrich-Loeffler-Institut

Andreas Hensel

Federal Institute for Risk Assessment

Helmut E. Meyer

University of Oslo

Heidrun Müller

Klinik und Poliklinik für Hals-, Nasen-, Ohrenheilkunde

Herbert Schmidt

University of Hohenheim

E. von Pritzbuer

University of Cologne

Cooperative Institutions

Institut für Hygiene und Umwelt

Institute of Medical Microbiology and Hygiene

University of Würzburg

Centers for Disease Control and Prevention

Institut Pasteur

Robert Koch Institute

Institut für Mikrobiologie der Bundeswehr

University of Cologne

Czech Academy of Sciences, Institute of Microbiology

Ludwig-Maximilians-Universität München

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Differentiation of Yersinia enterocolitica serotype O:5,27 strains by phenotypic and molecular techniques

Journal of Clinical Microbiology (1993)

Hiroshi Fukushima Manabu Gomyoda S Aleksić Misao Tsubokura

Restriction endonuclease analyses of virulence plasmid DNA (REAP) and chromosomal DNA and other phenotypic characteristics were used to study the differentiation of Yersinia enterocolitica serotype O:5,27 strains. There was a close correlation between REAP patterns and the geographical distribution of serotype O:5,27. Human isolates produced only one REAP pattern, which was also found with isolates from pigs and dogs.

Yersinia enterocolitica

Yersinia

Molecular Epidemiology

10.1128/jcm.31.6.1672-1674.1993

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Citations (34)

[Spectrum of gram-negative rods in waste water from Jeddah/Saudi Arabia].

PubMed (1985)

Müller He S Aleksić D Elsarnagawy Al-Mashhadi Ma J Bockemühl

23 samples of effluent of the wastewater treatment plant of Jeddah/Saudi Arabia were investigated between September 1983 and November 1984. A total of 395 strains of gram-negative fermentative bacteria were isolated and differentiated at species level. Neither vibrios, salmonellae, or shigellae were detected notwithstanding of enrichment procedures used. Furthermore, the occurrence of E. coli producing heat labile enterotoxin (LT) was studied using both Biken test and GM1-ELISA. The Biken test produced many false positive results which could not been confirmed by the more specific GM1-ELISA. All strains of E. coli investigated were negative. Therefore, the Biken test seems not to be a recommended method for detection of E. coli LT.

Gram

Gram-Negative Bacteria

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A Biotyping Scheme for Shiga-like (Vero) Toxin-producing Escherichia coli 0157 and a List of Serological Cross-reactions Between O157 and Other Gram-negative Bacteria's

Zentralblatt für Bakteriologie (1992)

S Aleksić Helge Karch J Bockemühl

10.1016/s0934-8840(11)80009-5

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Citations (72)

Occurrence and clinical importance of the pathogenic serogroup O:5, 27 of yersinia enterocolitica in the Federal Republic of Germany and methods for its serological and bacteriological identification

Zentralblatt für Bakteriologie Mikrobiologie und Hygiene Series A Medical Microbiology Infectious Diseases Virology Parasitology (1988)

S Aleksić J Bockemühl H Wuthe Verica Aleksić Sabo

Yersiniosis

Yersinia enterocolitica

Yersinia

Agglutination (biology)

Yersinia Infections

10.1016/s0176-6724(88)80096-8

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Serodiagnosis of human plague by an anti-F1 capsular antigen specific IgG/IgM ELISA and immunoblot

Epidemiology and Infection (2000)

Heinrich Neubauer Lila Rahalison Theoderic Brooks S Aleksić S Chanteau

Plague is a re-emerging disease endemic in at least 24 countries. Non-endemic countries should be able to confirm plague to prevent outbreaks due to imported cases. We established a combination of a IgG/IgM screening ELISA and a confirmation immunoblot employing F1 capsular antigen (CA) for the serodiagnosis of plague in countries where yersiniosis is present. The ELISA and the immunoblot assay showed a specificity of 96.1% and 100% among sera from healthy German blood donors. This group had a seroprevalence of 39% of anti-yersinia outer protein (YOP) antibodies obviously caused by previous Y. enterocolitica infection. The ELISA detected anti-F1 CA antibodies in 22 and the immunoblot in 20 out of 26 sera of plague vaccinees. Five control sera from bacteriologically confirmed plague cases from Madagascar reacted positively. It can be concluded that anti-YOP antibodies do not affect assays based on purified F1 CA.

Yersiniosis

plague

Seroprevalence

Yersinia pestis

Yersinia enterocolitica

10.1017/s0950268800004647

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Serovars and biovars of Levinea malonatica (syn. Citrobacter diversus, Citrobacter koserî) in clinical material.

Zentralblatt fur Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene, Abt. I (1981)

S Aleksić I Sechter A. Katz

Citrobacter

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Characterization and specificity controls of murine monoclonal antibodies against serogroup C1Salmonella

Diagnostic Microbiology and Infectious Disease (1992)

Raymond S. W. Tsang S Aleksić Kwok Hung Chan Nelson W.H. Lau M H Ng

Agglutination (biology)

10.1016/0732-8893(92)90116-b

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Isolation ofMoellerella wisconsensis from an infected human gallbladder

European Journal of Clinical Microbiology (1985)

Johann-Wolfgang Wittke S Aleksić H Wuthe

Isolation

10.1007/bf02013672

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Citations (16)

Comparison of restriction endonuclease analysis and phenotypic typing methods for differentiation of Yersinia enterocolitica isolates

Journal of Clinical Microbiology (1990)

Georg Kapperud Truls Nesbakken S Aleksić H.H. Mollaret

Restriction endonuclease analysis of chromosomal DNA (REAC) was used to study polymorphism in restriction fragment patterns among Yersinia enterocolitica isolates belonging to serogroups O3, O5,27, O8, O9, O13, and O21. Using the enzyme HaeIII and electrophoresis on thin (0.75-mm) vertical 5% polyacrylamide gels, we were able to distinguish at least 22 DNA fragment patterns among the 72 strains examined. The method showed the greatest discriminatory power with regard to serogroup O8, within which as many as 10 different DNA fragment patterns were detected among the 16 strains examined. Compared with O8, serogroups O3 and O9 were relatively homogeneous with regard to REAC patterns. The discriminatory power of the method was compared with H-antigen typing, biotyping, phage typing, antimicrobial susceptibility typing, and restriction enzyme analysis of the virulence plasmid (REAP), by means of Simpson's index of diversity. The results showed that REAC and REAP constitute an effective supplement or alternative to conventional phenotypic methods for tracing epidemiologically related isolates of Y. enterocolitica. Our finding that human and porcine isolates exhibited the same REAC, REAP, and H-antigen patterns provides additional support for the hypothesis that pigs play an important role in the epidemiology of human Y. enterocolitica infection.

HaeIII

Yersinia enterocolitica

Phage typing

Restriction fragment

DNA profiling

10.1128/jcm.28.6.1125-1131.1990

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Citations (86)

Two new flagellar antigens of Salmonella, z67 and z68, and revision of the antigen z56.

PubMed (1982)

S Aleksić J Bockemühl L. Le Minor R. Beaud

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Citations (1)