E. H. Chowdhury

University of Science and Technology Chittagong

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Rokshana Parvin

University of Chinese Academy of Sciences

Mohammad Rafiqul Islam

Shaheed Suhrawardy Medical College

Mohammed Nooruzzaman

Cornell University

M Giasuddin

Bangladesh Livestock Research Institute

M. R. Islam

University of Rajshahi

Md. Enamul Haque

Stanford University

H. Müller

Leipzig University

P. M. Das

Chattagram Maa-O-Shishu Hospital Medical College

M Noor

Universiti Putra Malaysia

Mohammad Mushfiqur Rahman

Purdue University West Lafayette

Cooperative Institutions

Bangladesh Agricultural University

Bangladesh Livestock Research Institute

University of Dhaka

Sher-e-Bangla Agricultural University

Bangabandhu Sheikh Mujib Medical University

Leipzig University

Chattogram Veterinary and Animal Sciences University

Aristotle University of Thessaloniki

Noakhali Science and Technology University

Bangabandhu Sheikh Mujibur Rahman Agricultural University

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IMMUNE RESPONSE OF GOATS TO THERMOSTABLE PPR VACCINE IN BANGLADESH

SAARC Journal of Agriculture (2011)

Mohammad Mushfiqur Rahman Ataur Rahman Bhuiyan Rokshana Parvin M Giasuddin Mahmoud Haque

To control peste des petits ruminants (PPR) in Bangladesh a live attenuated conventional PPR vaccine was developed by Bangladesh Livestock Research Institute (BLRI) and currently being used in the country. Farmers and field veterinarians often raise the question about the efficacy of that vaccine. The poor efficacy of PPR vaccine in rural situation was thought to be due to break in cold chain system during transportation. To address to this problem, a thermostable preparation of the former PPR vaccine has been made by BLRI. The present study was carried out to assess the efficacy of both the vaccines on the basis of the humoral immune response. A total of 190 goats more than 3 months of age of both sexes were selected for this experiment. Sera collected from experimental goats were analyzed with a competitive enzyme linked immunosorbent assay (cELISA). Percent inhibition (PI) value greater than 50% was considered as positive. In case of conventional PPR vaccine, it was found that 62% of the goats were sero-positive at 21 days post vaccination (DPV), which declined to 34.72 % at 180 DPV. On the other hand, in case of thermostable PPR vaccine, 70% serum samples were found positive at 21 DPV and that decreased slightly to 60% at 180 DPV. Kruskal-Wallis test and paired t- test revealed no significant difference in the antibody response of the thermostable vaccine kept at 25 , 30, 35 and 40 o C temperatures for 7 -14 days. Keyword: cELISA, immune response, thermostable PPR vaccine, storage time, temperature

Peste-des-Petits-Ruminants

Vaccine efficacy

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Molecular Analysis of Hexaploid Wheat (Triticum aestivum L.) Cultivars by RAPD Markers

Plant Tissue Culture and Biotechnology (1970)

Sevanthi Venkata Amitha Charu Rama Mitra K. M. Nasiruddin E. H. Chowdhury

RAPD assay was conducted for molecular genetic analysis of six wheat cultivars, such as, Kanchan, Sourav, Gourab, Shatabdi, Pavon and BAW-1006 to observe genetic variability and relatedness among these cultivars. Three out of 12 decamer random primers showed distinctly polymorphic bands when used to amplify genomic DNA. The primers yielded a total of 23 RAPD markers of which 14 were considered as polymorphic. The proportion of polymorphic loci and gene diversity (h) values were 34.78% and 0.153 for BAW-1006, 30.43% and 0.124 for Kanchan, 26.09% and 0.127 for Shatabdi, 26.09% and 0.127 for Pavon, 26.09% and 0.111 for Gourab, 21.74% and 0.098 for Sourav, respectively. The coefficient of gene differentiation (Gst) and gene flow (Nm) values across all the loci were 0.50 and 0.50, respectively indicating genetic divergence among populations. The UPGMA dendrogram based on Nei’s genetic distance, grouped six cultivars into two main clusters: Kanchan, Sourav, Gourab and Shatabdi in cluster I; Pavon and BAW-1006 in cluster II. The cluster I was further separated: Kanchan alone in sub-cluster I and Sourav, Gourab, Shatabdi in sub-cluster II; furthermore, Sourab and Gourab grouped together in sub-sub-cluster I of sub-cluster II with the lowest genetic distance of 0.035. Thus, RAPD offer a potentially simple, rapid and reliable method to evaluate genetic variation and relatedness among six wheat cultivars. Key words: RAPD, genetic diversity, polymorphic loci, wheat D.O.I. 10.3329/ptcb.v19i1.4915 Plant Tissue Cult. & Biotech. 19(1): 35-44, 2009 (June)

UPGMA

Dendrogram

Genetic distance

Genetic divergence

10.3329/ptcb.v19i1.4915

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Citations (3)

Male systemic lupus erythematosus, rapidly progressive glomerulonephritis, and iatrogenic acute bone marrow failure: a case report

International Journal of Scientific Reports (2024)

Utpal Das E. H. Chowdhury

Lupus nephritis (LN) is a significant complication of systemic lupus erythematosus (SLE), marked by kidney inflammation due to autoimmune activity, leading to proteinuria, hematuria, and potentially renal failure. Rapidly progressive glomerulonephritis (RPGN) is a rare, critical manifestation of LN characterized by a rapid decline in kidney function. This condition can lead to irreversible renal damage and is often fatal without prompt treatment. In this case, a 62-year-old man initially presented with fever, cough, and body aches, which were treated as an acute upper respiratory infection. Despite initial improvement, he developed persistent nausea, vomiting, and signs of renal dysfunction. Laboratory investigations revealed anemia, high erythrocyte sedimentation rate (ESR), electrolyte imbalances, and elevated creatinine. Imaging and endoscopy ruled out malignancy, and a differential diagnosis of multiple myeloma was excluded through plasma protein electrophoresis. Serological tests confirmed SLE, and subsequent renal biopsy revealed LN with RPGN features. Despite aggressive treatment with corticosteroids and cyclophosphamide, the patient’s condition rapidly deteriorated, leading to respiratory distress and intensive care unit (ICU) admission. He ultimately succumbed to his illness, underscoring the unpredictable and severe nature of RPGN in LN. This case highlights the importance of early diagnosis and intensive management to prevent rapid disease progression and improve patient outcomes.

Rapidly progressive glomerulonephritis

10.18203/issn.2454-2156.intjscirep20243054

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Development of an indirect ELISA for detection of antibody against peste des petits ruminants virus in sheep

Bangladesh Journal of Veterinary Medicine (2019)

Fardina Sumi Mohammed Nooruzzaman Mohammad Mushfiqur Rahman M. R. Islam E. H. Chowdhury

Background: Emergence of exotic viral disease like peste des petits ruminants(PPR) possesses a great threat towards successful goat and sheep farming in Bangladesh. Implementation of effective control measures against PPR requires a proper and rapid diagnosis of the disease. Various diagnostic techniques are available for diagnosis of PPR in goats and sheep in Bangladesh. Among these, ELISA is an important technique to diagnose the disease as well as for seromonitoring. However, the ELISA kits that are being used in the country are mostly imported. This study was undertaken to develop an in-house indirect ELISA using local PPR virus isolate as an antigen.Methods: A local isolate of PPR virus (PPRV) was used as an antigen to develop an indirect ELISA kit. Standardization of the optimum antibody, conjugate and virus antigen concentration was performed using chequer-board titration with different dilutions of the reagents. Finally, the developed indirect ELISA kit was used in a pilot study to detect anti-PPRV antibodies in sheep sera samples.Results: A polyclonal antibody based indirect ELISA was developed successfully for the detection of PPRV antibodies. The developed ELISA could detect anti-PPRV antibody in sheep sera sample during our pilot field trial.Conclusions: After robust field validation, the indirect ELISA can be used to determine the antibody titer against PPRV in the field as to monitor the seroconversion after vaccination.

Peste-des-petits-ruminants virus

Peste-des-Petits-Ruminants

Serial dilution

Polyclonal antibodies

10.33109/bjvmjj19fam3

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Citations (1)

Genetic characterization of highly pathogenic H5N1 avian influenza virus from live migratory birds in Bangladesh

Virus Genes (2014)

Rokshana Parvin Abu Hena Mustafa Kamal Md. Enamul Haque E. H. Chowdhury Mohammed Giasuddin

Highly pathogenic

Bird nest

10.1007/s11262-014-1118-0

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Citations (17)

Pathology of an outbreak of highly pathogenic avian influenza A(H5N1) virus of clade 2.3.2.1a in turkeys in Bangladesh

Journal of Veterinary Diagnostic Investigation (2020)

Tanjin Tamanna Mumu Mohammed Nooruzzaman Azmary Hasnat Rokshana Parvin E. H. Chowdhury

A mixed-aged flock of 130 turkeys in Bangladesh reported the sudden death of 1 bird in September 2017. Highly pathogenic avian influenza A(H5N1) virus was detected in 3 turkeys, and phylogenetic analysis placed the viruses in the reassortant clade 2.3.2.1a. The birds had clinical signs of depression, diarrhea, weakness, closed eyes, and finally death. The mortality rate of the flock was 13% over the 6 d prior to the flock being euthanized. At autopsy, we observed congestion in lungs and brain, hemorrhages in the trachea, pancreas, breast muscle, coronary fat, intestine, bursa of Fabricius, and kidneys. Histopathology revealed hemorrhagic pneumonia, hemorrhages in the liver and kidneys, and hemorrhages and necrosis in the spleen and pancreas. Significant changes in the brain included gliosis, focal encephalomalacia and encephalitis, and neuronophagia.

Flock

Histopathology

10.1177/1040638720965540

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Citations (6)

Development of multiplex reverse transcription polymerase chain reaction (RT-PCR) for simultaneous detection of matrix, haemagglutinin and neuraminidase genes of H5N1 avian influenza virus

Bangladesh Veterinarian (2012)

R. Mojumder E. H. Chowdhury Rokshana Parvin Jahan Ara Begum M Giasuddin

Influenza A virus, subtype H5N1 causes a fatal disease in domestic poultry and could spread directly from poultry to humans. The aim of this study was to develop a multiplex reverse transcription polymerase chain reaction (mRT-PCR) for simultaneous detection of Type A influenza virus-specific matrix protein (M) gene as well as H5 and N1 genes of highly pathogenic avian influenza (HPAI) viruses. Finnzymes Phusion-Flash High- Fidelity PCR Master Mix (Finnzymes Oy, Finland) and Qiagen one-step RT-PCR enzyme mix (Qiagen, Germany) were used in a one-step RT-PCR. RNA was extracted from two known positive samples using Qiagen RNA extraction kit. RT-PCR was carried out with a mixture of primers specific for the Type A influenza virus matrix protein (M), and H5 and N1 genes of H5N1 HPAI viruses in a single reaction system. The mRT-PCR cDNA products were visualized by gel electrophoresis. The mRT-PCR yielded fragments of 245 bp for M, 545 bp for H5 and 343 bp for N1 genes of HPAI virus, which were clearly distinguishable. The mRT-PCR using the Finnzymes Phusion-Flash High-Fidelity PCR Master Mix (Finnzymes Oy, Finland) with Qiagen one-step RT-PCR Enzyme Mix (Qiagen, Germany) required only one hour and 20 minutes. (Bangl. vet. 2011. Vol. 28, No. 2, 55 59)DOI: http://dx.doi.org/10.3329/bvet.v28i2.10653

Multiplex

Trizol

10.3329/bvet.v28i2.10653

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Citations (4)

Superior performance of the Brahma cattle in terms of meat production and disease resistance in comparison with the indigenous cattle of Bangladesh

Bangladesh Journal of Veterinary Medicine (2021)

M. Ahammed Tasmina Akter Mohammed Nooruzzaman Mohammad Rashedul Alam Md. Tanvir Islam

Background: The Brahma is a breed of cattle which grows very rapidly and suitable for high quality meat production. The breed originating from native cattle dwelling at unfavorable climate of India has adapted with scarcity of food, external and internal parasitism, infectious diseases and weather extremities. There is very limited study on adaptation and performance of Brahma cattle particularly in the context of Bangladesh where meat production is a crying demand. In this study, we evaluated the comparative performance of Brahma and indigenous cattle in terms of body weight gain and disease resistance in Bangladesh. Methods: A total of 10 Brahma calves and 10 indigenous calves were monitored for a period of 18 months during June 2018 to November 2020. Body weight gain and occurrence of diseases in both breeds were recorded. Two-way repeated measure ANOVA with Bonferroni post-tests was performed to compare the growth of both Brahma and indigenous calves. Results: At birth, both Brahma (15.75±0.19 kg) and indigenous (17.55±0.35 kg) calves had overall similar body weight. However, the Brahma calves gained significantly higher body weight than the indigenous calves over the next months. For example, the Brahma calves had higher body weight at 6 months (85.5±2.50 kg vs 70.75±0.43 kg), 12 months (160.3 kg±5.667 vs 120.4±0.89 kg) and 18 months (237.5±4.58 kg vs 169.8±1.72 kg) than the indigenous calves. An average daily body weight gain was 410.6±7.7 g and 282±2.9 g for Brahma and indigenous calves, respectively. Analysis of the disease records also showed a decreased number of diseases in Brahma than the indigenous cattle. Conclusion: Our study indicated a better adaptation and growth of Brahma cattle in comparison to the indigenous breed. Therefore, the Brahma breed can be a very promising source to reduce the deficiency of animal proteins in Bangladesh.

10.33109/bjvmjd2020rm5

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Citations (1)

Molecular evolution of H5N1 highly pathogenic avian influenza viruses in Bangladesh between 2007 and 2012

Avian Pathology (2014)

Md. Enamul Haque M Giasuddin E. H. Chowdhury Mohammad Rafiqul Islam

In Bangladesh, highly pathogenic avian influenza (HPAI) virus subtype H5N1 was first detected in February 2007. Since then the virus has become entrenched in poultry farms of Bangladesh. There have so far been seven human cases of H5N1 HPAI infection in Bangladesh with one death. The objective of the present study was to investigate the molecular evolution of H5N1 HPAI viruses during 2007 to 2012. Partial or complete nucleotide sequences of all eight gene segments of two chicken isolates, five gene segments of a duck isolate and the haemagglutinin gene segment of 18 isolates from Bangladesh were established in the present study and subjected to molecular analysis. In addition, full-length sequences of different gene segments of other Bangladeshi H5N1 isolates available in GenBank were included in the analysis. The analysis revealed that the first introduction of clade 2.2 virus in Bangladesh in 2007 was followed by the introduction of clade 2.3.2.1 and 2.3.4 viruses in 2011. However, only clade 2.3.2.1 viruses could be isolated in 2012, indicating progressive replacement of clade 2.2 and 2.3.4 viruses. There has been an event of segment re-assortment between H5N1 and H9N2 viruses in Bangladesh, where H5N1 virus acquired the PB1 gene from a H9N2 virus. Point mutations have accumulated in Bangladeshi isolates over the last 5 years with potential modification of receptor binding site and antigenic sites. Extensive and continuous molecular epidemiological studies are necessary to monitor the evolution of circulating avian influenza viruses in Bangladesh.

H5N1 genetic structure

Highly pathogenic

10.1080/03079457.2014.898244

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Citations (20)

Corrigendum to “Experimental infection of pregnant ewes with enteric and abortion-source Chlamydophila abortus” [Vet. Microbiol. 82 (2001) 285–291]

Veterinary Microbiology (2002)

P. TSAKOS Victoria I. Siarkou Franco Guscetti E. H. Chowdhury N. Papaioannou

Enteric fever

10.1016/s0378-1135(01)00520-x

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