Tetsuo Nozaki

Kushiro City General Hospital

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Hideki Fuse

University of Toyama

Yasuyoshi Fujiuchi

University of Toyama

Akihiro Morii

Kushiro City General Hospital

Akira Komiya

Chiba University

Takashi Kondo

RIKEN Center for Integrative Medical Sciences

Akihiko Watanabe

Kyushu Institute of Technology

Ryohei Ogawa

University of Toyama

Akiou Okumura

Kushiro City General Hospital

Kenji Yasuda

Waseda University

Hiroaki Iida

University of Toyama

Cooperative Institutions

University of Toyama

Tohoku Medical and Pharmaceutical University

University of Verona

Azienda Ospedaliera Universitaria Integrata Verona

University of Padua

Nippon Medical School

Hospital Doctor José Molina Orosa

Toyama University Hospital

Ospedale Maggiore

Chinese Academy of Sciences

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133 Enhancement of Ultrasound-Mediated Transfection by the Combination of Cavitation Facilitation and Plasma Membrane Modification(Effects of electro-magnetic fields, Abstracts of the 46th Annual Meeting of the Japan Radiation Research Society)

Journal of Radiation Research (2003)

Ryohei Ogawa Tetsuo Nozaki Go Kagiya Loreto B. Feril Hideki Fuse

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Effects of microbubbles on ultrasound-mediated gene transfer in human prostate cancer PC3 cells: Comparison among Levovist, YM454, and MRX-815H

Cancer Letters (2008)

Akihiko Watanabe Remon Otake Tetsuo Nozaki Akihiro Morii Ryohei Ogawa

Sonication

Gene transfer

10.1016/j.canlet.2008.02.004

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Citations (21)

Retroperitoneoscopic Nephrectomy for Symptomatic Hydronephrosis Using a SAND Balloon Catheter

Journal of Laparoendoscopic & Advanced Surgical Techniques (2011)

Tetsuo Nozaki Yoshihiro Asao Takatoshi Ito Shinji Tsuritani Akiou Okumura

Introduction: A retroperitoneoscopic nephrectomy (RN) for symptomatic hydronephrosis (SH) is a challenging procedure because of the limited working space. This report describes a specific technical modification for efficient and successful RN for SH by using the SAND balloon catheter. Patient and Methods: A 38-year-old woman underwent RN for SH caused by extrinsic compression of the ureter by a pelvic endometriosis. The SAND balloon catheter was directly inserted into the expanded hydronephrotic sac, and the liquid was extracted, appropriately. Urine leakage from the hydronephrotic sac could be avoided because the puncture site was sealed firmly between the two adjacent balloons at the tip of the catheter. Disposal counter traction using the catheter facilitated the mobilization of the hydronephrotic sac. Results: The patient was discharged 3 days after undergoing this procedure. Conclusions: This method improves the surgeon's vision and facilitates resection without causing any injury to the hydronephrotic sac wall during RN.

Balloon catheter

10.1089/lap.2010.0295

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Citations (1)

Enhancement of ultrasound‐mediated gene transfection by membrane modification

The Journal of Gene Medicine (2003)

Tetsuo Nozaki Ryohei Ogawa Loreto B. Feril Go Kagiya Hideki Fuse

Abstract Background Ultrasound‐mediated gene transfection (USMGT) with an echo contrast agent could be a new promising physical method of triggering localized gene delivery, but the effect is still modest. The aim of this study is to devise a method to improve efficiency of USMGT. We examined the effect of lidocaine and different temperatures on USMGT, each of which is a known membrane modifier, since the plasma membrane can be considered a site of action in USMGT. Methods We observed the effect of lidocaine (0.01, 0.1 or 1.0 mM) and different temperatures (7, 20, 37, 42 or 44 °C) on USMGT (1 MHz, 3.6 W/cm 2 (I SATA ) and 20 s exposure) in the presence of Levovist (10 mg/ml). At 20 h after sonication, transfection efficiency was evaluated by luciferase assay. Membrane fluidity was examined by fluorescence polarization measurement. Cavitational activity was measured by ESR spin trapping with 5,5‐dimethyl‐1‐pyrroline N ‐oxide. The number of cells transfected with the GFP gene was counted under a fluorescence microscope. Results Lidocaine (1 mM) and heat (42–44 °C) significantly increased luciferase expression approximately 18‐fold and 19‐fold higher than Levovist only. Both treatments were shown to increase membrane fluidity; in addition, heat enhanced a cavitational effect. It was confirmed by an experiment using the GFP gene that increase in luciferase expression was due to the increase in number of cells. Conclusions This enhancement could be useful for ultrasound‐mediated gene therapy in the future since both treatments for membrane modification could be directly applied to the living body. Copyright © 2003 John Wiley & Sons, Ltd.

Sonication

Sonoporation

10.1002/jgm.445

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Small cell carcinoma of the prostate after high-dose-rate brachytherapy for low-risk prostatic adenocarcinoma

Oncology Letters (2012)

Akira Komiya Kenji Yasuda Tetsuo Nozaki Yasuyoshi Fujiuchi Shinichi Hayashi

In the present study, we describe an 80-year-old patient who developed prostatic small cell carcinoma (SCC) following high-dose-rate brachytherapy (HDR-BT) for low-risk prostatic adenocarcinoma. The patient received one implant of Ir-192 and 7 fractions of 6.5 Gy within 3.5 days, for a total prescribed dose of 45.5 Gy. A total of 27 months after HDR-BT, the patient complained of difficulty in urinating. His serum prostate-specific antigen (PSA) levels were 3.2 ng/ml. Systemic examination revealed an enlargement of the prostate, urethral stenosis, pelvic lymph node swelling and multiple lung and bone lesions. His serum neuron-specific enolase (NSE) levels were elevated to 120 ng/ml. A prostate needle biopsy was performed for pathological examination. Histologically, there were tumor cells with hyperchromatic nuclei and scant cytoplasm showing a solid or trabecular growth pattern. Immunohistochemically, they were positive for AE1/AE3, CD56 and synaptophysin, and negative for PSA, PAP and CD57. These findings are consistent with SCC of the prostate. A review of the prostate needle biopsy specimen prior to HDR-BT did not reveal any tumor cells positive for chromogranin A, nor synaptophysin. The final diagnosis was SCC of the prostate with local progression, with lung, lymph node and bone metastases. Three cycles of etoposide/cisplatin (EP) were administered. A greater than 50% decrease in the serum NSE levels was observed. However, there was no objective response. Due to the deterioration of the patient's general condition, EP was discontinued. One month later, his serum NSE showed a rapid increase to 210 ng/ml with aggressive local progression and the patient succumbed to the disease 5.5 months after the start of EP therapy.

Chromogranin A

Synaptophysin

10.3892/ol.2012.998

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Percutaneous endopyelotomy for ureteropelvic junction obstruction.

International Urology and Nephrology (2002)

Akiou Okumura Hideki Fuse Shinji Tsuritani Tetsuo Nozaki

Nephrology

10.1023/a:1025606600089

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Selective Renal Parenchymal Clamping in Retroperitoneal Partial Nephrectomy

Journal of Laparoendoscopic & Advanced Surgical Techniques (2012)

Tetsuo Nozaki Hiroaki Iida Akihiro Morii Yasuyoshi Fujiuchi Hideki Fuse

Objective: Herein, we report our experience with retroperitoneoscopic partial nephrectomy (RPN) without hilar occlusion by the use of a laparoscopic clamp to induce selective regional ischemia. Methods: A 48-year-old woman was referred for a left upper polar renal mass, which was suspected to be malignant. The contralateral kidney revealed severe atrophy, and she was scheduled to undergo RPN using a laparoscopic clamp to induce selective regional ischemia. At first, the kidney is fully mobilized within the retroperitoneal space. Thereafter, the laparoscopic clamp is applied directly to the kidney, about 1 cm below the resection line. When closed, the renal parenchyma is compressed, so that blood supply to the tumor is interrupted. The preserved portion of the kidney is perfused normally, and it is possible to remove the tumor in a bloodless field without involving warm ischemia. Results: Renal hilar clamping was avoided, with minimal estimated blood loss. There was no perioperative complication, and the final pathology revealed a hemorrhagic renal cyst. The radioisotope absorption of the enucleated kidney was well maintained, except for the marginal area of the enucleated site. The renogram pattern was found to be equivocal when compared with the preoperative renogram. Conclusion: Regional renal parenchymal clamping during RPN can be safely and effectively used to create a bloodless operative field. Moreover, our preliminary experience demonstrates that this technique facilitates maximal nephron-sparing surgery for patients with an anatomically or functionally solitary kidney, without involving warm ischemia.

Clamp

Parenchyma

Renal hilum

Nephrology

Renal ischemia

10.1089/lap.2011.0245

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Citations (4)

Laparoscopic ureterolysis for idiopathic retroperitoneal fibrosis

International Journal of Urology (2005)

Akiou Okumura Kouichi Murakami Tetsuo Nozaki Hideki Fuse

Three patients with idiopathic retroperitoneal fibrosis underwent laparoscopic ureterolysis. Two patients were bilateral cases which were performed successfully as a one stage procedure. Another patient, who was unsuccessfully treated, had a long ureteral stricture. Laparoscopic ureterolysis may be a useful alternative to open surgical management especially in bilateral cases, except for patients with a long ureteral stricture.

Ureterolysis

10.1111/j.1442-2042.2005.01214.x

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Citations (18)

Glove-Assisted Laparoscopic Radical Nephrectomy: A Novel Technique

Journal of Laparoendoscopic & Advanced Surgical Techniques (2010)

Tetsuo Nozaki Yoshihiro Asao Keisuke Ichimatsu Takatoshi Ito Kenji Yasuda

Laparoscopic surgery has not yet met with widespread acceptance due to its degree of technical difficulty. The laparoscopic radical nephrectomy procedure was improved with the aid of an abdominal wall sealing device, a wound retractor, and a surgical glove.A 5 cm skin incision was made at the beginning of the operation. The Alexis wound retractor S was set up through this small incision. The wrist portion of the surgical glove (size 8-0) was then used to cover the outer ring of the wound retractor to maintain pneumoperitoneum. The surgeon can use most of the usual surgical instruments through the wound retractor during the laparoscopic surgery.These procedures were successfully conducted in all cases without open conversion, and no postoperative complications were observed.Glove-assisted laparoscopic surgery can be used to perform advanced laparoscopic procedures. This new technique made laparoscopic abdominal surgery easier and safer for beginners in laparoscopic surgery and skilled surgeons in open surgery.

Retractor

Open surgery

10.1089/lap.2010.0294

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Citations (1)

Use of the Laparoscope Holder for Liver Retraction during Urological Laparoscopic Surgery

Current Urology (2012)

Tetsuo Nozaki Hiroaki Iida Akihiro Morii Yasuyoshi Fujiuchi Akira Komiya

During laparoscopy, as in open surgery, exposure is critical. Here, we describe the use of a laparoscope holder to facilitate the liver lift during urological laparoscopic surgery.Laparoscopic right radical nephrectomy (n = 3), partial nephrectomy (n = 1), and adrenalectomy (n = 2) were performed with 4 ports. At the beginning of the operation, the small snake retractor was placed through the 5-mm port under direct vision and the liver was lifted in the appropriate direction to optimize exposure.The laparoscope holder provided quick, reproducible retraction to facilitate exposure. No complications occurred with its use. The device reduced the need for a dedicated second assistant to stand bedside.We achieved significant improvements in the safety and efficiency of liver retraction during urological laparoscopic surgery using the laparoscope holder.

Retractor

Port (circuit theory)

Open surgery

10.1159/000343518

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Citations (7)