Augustine T. Peter

Apoptosis of the ovarian granulosa cells plays a crucial role in the determination of the number of follicles destined to ovulate in each reproductive cycle. While the activation of specific apoptotic pathway or the inactivation of cell survival pathway can initiate apoptosis, the signaling mechanism(s) involved in initiating the onset of apoptosis in granulosa cells is not fully understood. In the present study, using granulosa cells derived from eCG-primed immature rats, we investigated the temporal signaling events involved in the onset of apoptosis in the granulosa cells. The administration of 15 IU of eCG to 21-day-old immature female rats stimulate the growth and development of ovarian follicles until 72 h, after which the granulosa cells of the ovarian follicles undergo apoptosis due to the waning levels of tropic hormonal support. An analysis of the signaling events leading to apoptosis indicates that the DNA fragmentation can be seen in these cells from 96 h. A small increase in the levels of the pro-apoptotic factor Bax can be seen from 96 h while an increase in the activity of JNK can be seen from 108 h onwards. By contrast, a reduction in ERK signaling can be seen by 48 h. Similar reduction in Raf-1 kinase activity can be discerned from 48 h onwards. A concomitant decrease in the phosphorylated form of Bad can also be detected. These findings taken together, suggest that the loss of tropic hormone support is translated into the attenuation of Raf-1-MEK-ERK signaling pathway and this reduction along with a reduction in the levels of phosphorylated form of Bad triggers the onset of apoptosis in the ovarian granulosa cells.

Granulosa cell

10.1002/(sici)1097-4644(19991215)75:4<547::aid-jcb1>3.0.co;2-5

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Citations (54)

Terminologies for the pre-attachment bovine embryo

Theriogenology (2011)

Jaana Peippo Zoltán Macháty Augustine T. Peter

10.1016/j.theriogenology.2011.06.018

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Citations (28)

Apoptosis of ovarian granulosa cells: Correlation with the reduced activity of ERK‐signaling module

Journal of Cellular Biochemistry (1999)

Gerd Gebauer Augustine T. Peter Djamila Onésime N. Dhanasekaran

Apoptosis of the ovarian granulosa cells plays a crucial role in the determination of the number of follicles destined to ovulate in each reproductive cycle. While the activation of specific apoptotic pathway or the inactivation of cell survival pathway can initiate apoptosis, the signaling mechanism(s) involved in initiating the onset of apoptosis in granulosa cells is not fully understood. In the present study, using granulosa cells derived from eCG-primed immature rats, we investigated the temporal signaling events involved in the onset of apoptosis in the granulosa cells. The administration of 15 IU of eCG to 21-day-old immature female rats stimulate the growth and development of ovarian follicles until 72 h, after which the granulosa cells of the ovarian follicles undergo apoptosis due to the waning levels of tropic hormonal support. An analysis of the signaling events leading to apoptosis indicates that the DNA fragmentation can be seen in these cells from 96 h. A small increase in the levels of the pro-apoptotic factor Bax can be seen from 96 h while an increase in the activity of JNK can be seen from 108 h onwards. By contrast, a reduction in ERK signaling can be seen by 48 h. Similar reduction in Raf-1 kinase activity can be discerned from 48 h onwards. A concomitant decrease in the phosphorylated form of Bad can also be detected. These findings taken together, suggest that the loss of tropic hormone support is translated into the attenuation of Raf-1-MEK-ERK signaling pathway and this reduction along with a reduction in the levels of phosphorylated form of Bad triggers the onset of apoptosis in the ovarian granulosa cells. J. Cell. Biochem. 75:547–554, 1999. © 1999 Wiley-Liss, Inc.

Granulosa cell

10.1002/(sici)1097-4644(19991215)75:4<547::aid-jcb1>3.3.co;2-x

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Citations (5)

Relaxin: A hormonal aid to diagnose pregnancy status in wild mammalian species

Theriogenology (2014)

D.R. Bergfelt Augustine T. Peter M.A. Beg

Relaxin

Rhinoceros

10.1016/j.theriogenology.2014.07.030

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Citations (13)

Andrology laboratory review: evaluation of sperm motility

Clinical Theriogenology (2021)

Augustine T. Peter Leonardo F.C. Brito Gary C. Althouse Christine Aurich P.J. Chenoweth

This review is an effort by the andrology taskforce of the Association of Applied AnimalAndrology, American College of Theriogenologists, European College of Animal Reproduction, andSociety for Theriogenology. It is intended to serve as a reference on methods to evaluate spermmotility in domestic animals and to contribute to adoption of best practices in veterinary andrologylaboratories and semen processing centers. Sperm motility evaluation topics covered include samplepreparation, subjective evaluation, computer-assisted semen analysis, and use of sperm qualityanalyzers. Emphasis is given to principles of the methods, equipment, performing evaluation, andcommon mistakes and/or pitfalls. In addition, precision and accuracy of the various methods arediscussed.

Theriogenology

Semen Analysis

10.58292/ct.v13.9359

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Citations (8)

Assessment of ovarian follicular dynamics and folliculogenesis associated endocrine profiles following gonadotropin stimulation in the bonnet monkey

General and Comparative Endocrinology (2017)

Bhupathi Sukesh Muraly Puttabyatappa Augustine T. Peter R. Medhamurthy Polani B. Seshagiri

Antral follicle

Gonadotropin

Human chorionic gonadotropin

10.1016/j.ygcen.2017.08.016

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Citations (1)

Male pseudohermaphroditism of the testicular feminization type in a heifer.

PubMed (1993)

Augustine T. Peter A. B. Scheidt Joseph W. Campbell Kevin A. Hahn

Male pseudohermaphroditism

Testicular feminization

Feminization (sociology)

Pseudohermaphroditism

Source

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Citations (3)

Clinical Theriogenology is breaking new ground!

Clinical Theriogenology (2023)

Augustine T. Peter

Theriogenology

10.58292/ct.v15.9601

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Citations (0)

Astaxanthin present in the maturation medium reduces negative effects of heat shock on the developmental competence of porcine oocytes

Reproductive Biology (2015)

Lanh Thi Kim V. V. Luu Yasuhiro Morita Masayasu Taniguchi Masahiro Nii

In vitro maturation

10.1016/j.repbio.2015.01.002

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Citations (62)

Management of Vaginal, Cervico‐Vaginal, and Uterine Prolapse

Bovine Reproduction (2021)

Augustine T. Peter E. Heath King

Eversion of the vagina or both uterine horns from the vulva is commonly referred to as vaginal or uterine prolapse, respectively. It is not unusual to encounter these cases in cattle, particularly in the developing world. Vaginal and cervical eversions usually occur before calving and uterine eversions after calving. This chapter addresses this condition from three perspectives: describes these maladies and provide remedies with representative images, both real and created; provides a summary of information reported in the global literature, including practical solutions provided by practitioners; and addresses these clinical situations from productive and fertility perspectives and, most importantly, from an animal welfare perspective. Vaginal eversions are classified into four grades according to severity of eversion, extent of injury, and exposure of the cervix in such eversions. The objective of treatment in pregnant animals is to replace and retain the vagina and cervix within the pelvic canal and to deliver and wean a live offspring.

Uterine prolapse

10.1002/9781119602484.ch47

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Citations (2)

Augustine T. Peter

Papers

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