Frédéric Sohm

Centre National de la Recherche Scientifique

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Joanne Edouard

Institut de Biologie Intégrative de la Cellule

Jean‐Stéphane Joly

Institut des Neurosciences Paris-Saclay

Laurent Legendre

Centre National de la Recherche Scientifique

Florence Le Gac

Genopole (France)

Patrick Prunet

Fish Physiology and Genomics Institute

Marc Edery

Centre National de la Recherche Scientifique

Sylvie Rétaux

Institut des Neurosciences Paris-Saclay

Amaury de Luze

Evolution des Régulations Endocriniennes

Jean‐Jacques Lareyre

Fish Physiology and Genomics Institute

Sosthène Barbachou

Centre François Baclesse

Cooperative Institutions

Centre National de la Recherche Scientifique

Inserm

Université Paris-Saclay

Université Paris-Sud

Université Paris Cité

Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement

Institut des Neurosciences Paris-Saclay

Sorbonne Université

Fish Physiology and Genomics Institute

Institut National de la Recherche Agronomique

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A Point Mutation in the Mitochondrial Cytochrome b Gene Obviates the Requirement for the Nuclear Encoded Core Protein 2 Subunit in the Cytochrome bc1 Complex in Saccharomyces cerevisiae

Journal of Biological Chemistry (1997)

Jean‐Paul di Rago Frédéric Sohm Claire Boccia Geneviève Dujardin Bernard L. Trumpower

A yeast mutant (cor2-45) in which approximately half of the C terminus of core protein 2 of the cytochrome bc1 complex is lacking due to a frameshift mutation that introduces a stop at codon 197 in the COR2 gene fails to assemble the cytochrome bc1 complex and does not grow on non-fermentable carbon sources that require respiration. The loss of respiration is more severe with this frameshift mutation than with the complete deletion of the COR2 gene, suggesting deleterious effects of the truncated core 2 protein. A search for extragenic suppressors of the nuclear cor2-45 mutation resulted (in addition to the expected nuclear suppressors) in the isolation of a suppressor mutation in the mitochondrial DNA that replaces serine 223 by proline in cytochrome b.Assembly of the cytochrome bc1 complex and the respiratory deficient phenotype of the cor2-45 mutant are restored by the proline for serine replacement in cytochrome b. Surprisingly, this amino acid replacement in cytochrome b corrects not only the phenotype resulting from the cor2-45 frameshift mutation, but it also obviates the need for core protein 2 in the cytochrome bc1 complex since it alleviates the respiratory deficiency resulting from the complete deletion of the COR2 gene. This is the first report of a homoplasmic missense point mutation of the mitochondrial DNA acting as a functional suppressor of a mutation located in a nuclear gene and the first demonstration that the supernumerary core protein 2 subunit is not essential for the electron transfer and energy transducing functions of the mitochondrial cytochrome bc1 complex. A yeast mutant (cor2-45) in which approximately half of the C terminus of core protein 2 of the cytochrome bc1 complex is lacking due to a frameshift mutation that introduces a stop at codon 197 in the COR2 gene fails to assemble the cytochrome bc1 complex and does not grow on non-fermentable carbon sources that require respiration. The loss of respiration is more severe with this frameshift mutation than with the complete deletion of the COR2 gene, suggesting deleterious effects of the truncated core 2 protein. A search for extragenic suppressors of the nuclear cor2-45 mutation resulted (in addition to the expected nuclear suppressors) in the isolation of a suppressor mutation in the mitochondrial DNA that replaces serine 223 by proline in cytochrome b. Assembly of the cytochrome bc1 complex and the respiratory deficient phenotype of the cor2-45 mutant are restored by the proline for serine replacement in cytochrome b. Surprisingly, this amino acid replacement in cytochrome b corrects not only the phenotype resulting from the cor2-45 frameshift mutation, but it also obviates the need for core protein 2 in the cytochrome bc1 complex since it alleviates the respiratory deficiency resulting from the complete deletion of the COR2 gene. This is the first report of a homoplasmic missense point mutation of the mitochondrial DNA acting as a functional suppressor of a mutation located in a nuclear gene and the first demonstration that the supernumerary core protein 2 subunit is not essential for the electron transfer and energy transducing functions of the mitochondrial cytochrome bc1 complex.

Cytochrome C1

Suppressor mutation

10.1074/jbc.272.8.4699

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Citations (16)

Zebrafish: Housing and husbandry recommendations

Laboratory Animals (2019)

Peter Aleström Livia D’Angelo Paul J. Midtlyng Daniel F. Schorderet Stefan Schulte‐Merker

This article provides recommendations for the care of laboratory zebrafish (Danio rerio) as part of the further implementation of Annex A to the European Convention on the protection of vertebrate animals used for experimental and other scientific purposes, EU Commission Recommendation 2007/526/EC and the fulfilment of Article 33 of EU Directive 2010/63, both concerning the housing and care of experimental animals. The recommendations provide guidance on best practices and ranges of husbandry parameters within which zebrafish welfare, as well as reproducibility of experimental procedures, are assured. Husbandry procedures found today in zebrafish facilities are numerous. While the vast majority of these practices are perfectly acceptable in terms of zebrafish physiology and welfare, the reproducibility of experimental results could be improved by further standardisation of husbandry procedures and exchange of husbandry information between laboratories. Standardisation protocols providing ranges of husbandry parameters are likely to be more successful and appropriate than the implementation of a set of fixed guidance values neglecting the empirically successful daily routines of many facilities and will better reflect the wide range of environmental parameters that characterise the natural habitats occupied by zebrafish. A joint working group on zebrafish housing and husbandry recommendations, with members of the European Society for Fish Models in Biology and Medicine (EUFishBioMed) and of the Federation of European Laboratory Animal Science Associations (FELASA) has been given a mandate to provide guidelines based on a FELASA list of parameters, 'Terms of Reference'.

Animal Husbandry

European commission

Directive

10.1177/0023677219869037

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Citations (515)

Activation of gene transcription by tilapia prolactin variants tiPRL188 and tiPRL177

FEBS Letters (1998)

Frédéric Sohm Alain Pezet Olivier Sandra Patrick Prunet Amaury de Luze

In the tilapia species Oreochromis niloticus , the pituitary releases two forms of prolactins (tiPRL 188 and tiPRL 177 ). The binding parameters and the activation of tiPRL‐induced JAK2/Stat5 signalling pathway were analysed using a mammalian cell line transiently transfected with the tiPRL receptor (tiPRLR). Our data indicate that the tiPRLR is able to mediate transcriptional activation of the PRL responsive element. At nanomolar concentrations, tiPRL 188 activates gene transcription whereas at micromolar concentrations it inhibits luciferase transcription from the lactogenic responsive element. This is consistent with a model of receptor dimerisation. In contrast, the activation by tiPRL 177 was only reached at high (μM) concentrations. The transcriptional activities induced by tiPRL 177 and tiPRL 188 are discussed in the context of the physiology of these hormones.

Transcription

STAT5

Prolactin receptor

Response element

10.1016/s0014-5793(98)01285-x

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Citations (18)

The in vivo targeted mutation of the mineralocorticoid and glucocorticoid receptors in zebrafish (Danio rerio) provides evidence that both receptors have distinct and redundant effects on female reproduction

HAL (Le Centre pour la Communication Scientifique Directe) (2018)

Arthur Forget Frédéric Sohm Johanne Edouard Amélie Patinote Charlène Morton

Danio

Mineralocorticoid

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Isolation and expression of tilapia (Oreochromis niloticus) serine 8-type GnRH coding and regulatory sequences

Gene (2003)

Hamid Farahmand Md. Azizur Rahman Frédéric Sohm Gyu-Lin Hwang Norman Maclean

Nile tilapia

Coding region

10.1016/s0378-1119(02)01184-8

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Citations (9)

Presentation of DOGMATIS, an inter and multidisciplinary programme for the assessment of the impacts of Genetically Modified Fish, and results about risk of fortuitous import on french market.

HAL (Le Centre pour la Communication Scientifique Directe) (2010)

Mai-Ahn Ngo Bieng Sandrine Barrey Yves Bertheau Laurence Boy Léo Coutellec

DOGMATIS is a research project funded by the French Research Agency (ANR, programme ANR-OGM 2007-2010). The transgenic technologies have been applied to fish since more than 20 years now and some strains are at the premarket or market stage in countries outside Europe. In Europe the main risk is a fortuitous import. Any rumour of uncontrolled arrival of GM fish on the European market may have strong impacts on the market chain, the research and innovation system and the trust in public regulation. The aim of DOGMATIS is to anticipate the answers. It associates specialists of fish transgenesis, GMO detection and regulation, fish market chain economy, consumer sociology and contemporary science philosophy and epistemology. To tackle this multifactorial subject, we developed original methodologies including assumptions based on the quantitative and qualitative knowledge from the different experts of DOGMATIS. We propose to present the programme and the results of our investigation concerning an assessments of the risk of fortuitous presence of GM Fish on the French market, which has been done by crossing the data from scientific literature gathered in a data bank and an expert analysis of filtered statistics of international trade, for the two farmed species concerned by transgenesis techniques and imported on the French market, salmon and tilapia.

Transgenesis

Presentation (obstetrics)

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Postembryonic Fish Brain Proliferation Zones Exhibit Neuroepithelial-Type Gene Expression Profile

Stem Cells (2017)

Emilie Dambroise Matthieu Simion Thomas Bourquard Stéphanie Bouffard B Rizzi

Abstract In mammals, neuroepithelial cells play an essential role in embryonic neurogenesis, whereas glial stem cells are the principal source of neurons at postembryonic stages. By contrast, neuroepithelial-like stem/progenitor (NE) cells have been shown to be present throughout life in teleosts. We used three-dimensional (3D) reconstructions of cleared transgenic wdr12:GFP medaka brains to demonstrate that this cell type is widespread in juvenile and to identify new regions containing NE cells. We established the gene expression profile of optic tectum (OT) NE cells by cell sorting followed by RNA-seq. Our results demonstrate that most OT NE cells are indeed active stem cells and that some of them exhibit long G2 phases. We identified several novel pathways (e.g., DNA repair pathways) potentially involved in NE cell homeostasis. In situ hybridization studies showed that all NE populations in the postembryonic medaka brain have a similar molecular signature. Our findings highlight the importance of NE progenitors in medaka and improve our understanding of NE-cell biology. These cells are potentially useful not only for neural stem cell studies but also for improving the characterization of neurodevelopmental diseases, such as microcephaly.

Neuroepithelial cell

Cell type

Cell Sorting

Multipotent Stem Cell

10.1002/stem.2588

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Citations (16)

RESAMA: A Network for Monitoring Health and Husbandry Practices in Aquatic Research Facilities

Zebrafish (2016)

Laurent Legendre Brigitte Guillet Emmanuel Leguay Emmanuel Meunier Sophie Labrut

Health monitoring is a crucial aspect of the management of any research animal house. RESAMA is a network strong of 60 academic and private partners acting in France since the end of 2012. The network aims to increase awareness of animal caretakers and researchers on health management issues in facilities holding aquatic model species (zebrafish, Xenopus, medaka, Mexican tetra). To do so, each partner research facility will be visited at least once. The visiting team is composed at least of one veterinarian and one zootechnician specialized in aquatic species. The visit results in a health-monitoring assessment of the facility, which includes a sampling for histo-pathological, bacteriological, and molecular pathogen detection. During the visit, rearing practices are also reviewed through an interview of animal caretakers. However, the present report essentially focuses on the health-monitoring aspect. The ultimate goal of the project is to provide a network-wide picture of health issues in aquatic facilities. Performed in parallel, the rearing practice assessment will ultimately help to establish rational relationship between handling practices and animal health in aquatic facilities. The study is still in progress. Here, we describe the results to be drawn from an analysis of the 23 facilities that had been visited so far. We sampled 720 fish and 127 amphibians and performed a little less than 1400 individual tests.

Animal Husbandry

One Health

Animal health

10.1089/zeb.2015.1199

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Citations (7)

Simultaneous overexpression of GH and STAT5b genes inhibits the STAT5 signalling pathway in tilapia (Oreochromis niloticus) embryos

Genetics and Molecular Biology (2002)

Luís Fernando Marins Arati Iyengar Norman Maclean J.A. Levy Frédéric Sohm

In this study, we describe the use of a STAT5 responsive element (LHRE) reporter gene to monitor the activity of the growth hormone (GH) transduction pathway following expression of heterologous fish GH and rat STAT5b in tilapia embryos and fish fibroblast cells. Our results indicate that both GH and STAT5b are able to activate the LHRE at high levels when transferred separately, demonstrating the substantial level of conservation of the GH signal transduction pathways between fish and mammals. Unexpectedly, co-expression experiments show a strong inhibition of the GH-dependent activation, suggesting that simultaneous GH and STAT5b overexpression can counteract effects of GH expression in tilapia embryos.

STAT5

Heterologous

10.1590/s1415-47572002000300007

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Citations (10)

Génomique expressionnelle et fonctionnelle de la spermatogenèse chez la truite - Spermgen

HAL (Le Centre pour la Communication Scientifique Directe) (2010)

Florence Le Gac Jean‐Jacques Lareyre Rémi Houlgatte Michael Primig Frédéric Chalmel

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