P R Narayanan

National Institute of Research in Tuberculosis

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Periasamy Selvaraj

Emory University

Sujatha Narayanan

National Institute of Research in Tuberculosis

N Selvakumar

Anna University, Chennai

R Prabhakar

Kerala Veterinary and Animal Sciences University

A M Reetha

National Institute of Malaria Research

C N Paramasivan

National Institute of Research in Tuberculosis

T Santha

National Institute of Research in Tuberculosis

M S Jawahar

National Institute of Research in Tuberculosis

Sunil M. Kurian

Scripps Health

P G Gopi

Sound Health Options

Cooperative Institutions

National Institute of Research in Tuberculosis

113

Indian Council of Medical Research

All India Institute of Medical Sciences

National Institute of Allergy and Infectious Diseases

National Institutes of Health

University of Delhi

Creative Commons

University of Madras

Indian Institute of Science Bangalore

Madras Medical College

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Evaluation of ELISA as a diagnostic test in pulmonary tuberculosis

P R Narayanan G S Acharyulu Padmini Krishnamurthy Abdul Ravoof Srikanth Tripathy

The reproducibility and ability of ELISA to discriminate patients with active disease from those infected were investigated.. The results obtained on 60 sputum-positive adult tuberculous patients and 60 adult control subjects showed groupwise discrimination. The anti-PPD antibodies (Ig G class) were significantly elevated in tuberculous patients as compared with control subjects. But because of the large day-to-day variation and considerable overlap in the distribution of antibody levels in tuberculous and control subjects, it is not possible to use ELISA as a diagnostic tool for adult pulmonary tuberculosis.

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An analysis of in vitro B cell immune responsiveness in human lymphatic filariasis.

The Journal of Immunology (1987)

Thomas B. Nutman V. Kumaraswami Li‐Heng Pao P R Narayanan Eric A. Ottesen

Abstract The immunoregulatory mechanisms involved in B cell function in patients with varying clinical manifestations of bancroftian filariasis were examined by studying the ability of peripheral blood mononuclear cells (PBMC) or PBMC subpopulations from patients with elephantiasis, asymptomatic microfilaremia (MF), and acute tropical pulmonary eosinophilia (TPE) to produce polyclonal and parasite-specific antibody in vitro, both spontaneously and in response to a mitogen (PWM) and to parasite antigen. When the spontaneous or mitogen-driven polyclonal responses were examined, all groups produced significant amounts of IgM and IgG; those with TPE produced extremely high levels. However, when in vitro parasite antigen-specific responses were examined, those with MF were unable to produce filaria-specific antibody either spontaneously or in response to PWM or parasite antigen; in contrast, patients with chronic lymphatic obstruction or TPE produced large quantities. Removal of neither adherent cells nor T8+ T cells affected the parasite-specific B cell anergy seen in those with MF. This absent or severely diminished capacity to produce antibody on parasite antigenic stimulation in patients with MF is likely responsible for the low levels of parasite-specific antibody seen in this most common clinical manifestation of bancroftian filariasis. Its inability to be reversed by the removal of "suppressor elements" suggests a state of B cell unresponsiveness to the parasite.

Polyclonal antibodies

Brugia malayi

10.4049/jimmunol.138.11.3954

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Comparison of variants of carbol-fuchsin solution in Ziehl-Neelsen for detection of acid-fast bacilli.

PubMed (2005)

N Selvakumar M Gomathi Sekar Fathima Rahman A Syamsunder M Duraipandian

To evaluate Ziehl-Neelsen (ZN) staining using variants of carbol-fuchsin solution, duplicate smears from 416 samples were stained with ZN, one set with 1% basic fuchsin and the other 0.3%. Another set of duplicate smears from 398 samples were stained with ZN, one with 1% basic fuchsin and the other 0.1%. The coded smears were read and discrepancies resolved. All samples underwent mycobacterial culture. The sensitivity of ZN using 0.3% (65%) and 1% basic fuchsin (62%) was comparable, while it was reduced using 0.1% (74%) compared to 1% basic fuchsin (83%). Reducing the concentration of basic fuchsin below 0.3% in ZN staining was found to significantly reduce its sensitivity.

Ziehl–Neelsen stain

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Regulatory Role of Vitamin D Receptor Gene Variants of BsmI, ApaI, TaqI, and FokI Polymorphisms on Macrophage Phagocytosis and Lymphoproliferative Response to Mycobacterium tuberculosis Antigen in Pulmonary Tuberculosis

Journal of Clinical Immunology (2004)

Periasamy Selvaraj Girish Chandra M S Jawahar M. Girija Rani D. Nisha Rajeshwari

FokI

TaqI

10.1023/b:joci.0000040923.07879.31

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Intracellular granzyme A expression ofperipheral blood lymphocyte subsets inpulmonary tuberculosis

M. Vidyarani Periasamy Selvaraj M S Jawahar D. Rajeswari Selvakumar Anbalagan

Cell-mediated immunity is a key weapon of host defence against tuberculosis (TB). Granzyme A (GzmA), a serine protease, present in the granules of cytotoxic cells induces caspase-independent cell death. We estimated the proportion of GzmA producing lymphocyte subsets in peripheral blood from 59 normal healthy volunteers and 48 pulmonary TB (PTB) patients using flow cytometry. When compared with normal healthy subjects, we observed a significantly higher percentage of GzmA-positive CD56+ cells (P = 0.01) in PTB patients. However, when the absolute number was compared between the two groups, a significantly decreased number of GzmA-expressing CD16+ (P = 0.01) and CD56+ (P = 0.0001) cells was observed in patients and this could be explained by the significantly reduced number of total lymphocytes (P = 0.0009) seen in the patients. There was no significant difference in the number of CD4+ and CD8+ GzmA double-positive cells between the two study groups. CD56 is a natural killer cell marker and these cells represent innate immune response to TB. We report an increased percentage of CD56+ cells expressing GzmA in TB patients, which shows the relevance of the GzmA-mediated pathway of apoptosis in immunity against Mycobacterium tuberculosis.

Granzyme A

Granzyme

Granulysin

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Effect of Vitamin D3 on Phagocytic Potential of Macrophages withLive Mycobacterium tuberculosis and LymphoproliferativeResponse in Pulmonary Tuberculosis

Girish Chandra Periasamy Selvaraj M S Jawahar Banu Rekha P R Narayanan

Lymphoproliferative response

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Regulatory Role of Promoter and 3′ UTR Variants of Vitamin D Receptor Gene on Cytokine Response in Pulmonary Tuberculosis

Journal of Clinical Immunology (2008)

Periasamy Selvaraj M. Vidyarani Kalichamy Alagarasu S. Prabhu Anand P R Narayanan

10.1007/s10875-007-9152-5

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Estimation of annual risk of tuberculosis infection among children irrespective of BCG scar in the south zone of India.

Indian Journal of Tuberculosis (2006)

P G Gopi R Subramani C Kolappan V Venkatesh Prasad P R Narayanan

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Interleukin-12B & interleukin-10 gene polymorphisms in pulmonary tuberculosis.

PubMed (2007)

S. Prabhu Anand Periasamy Selvaraj M S Jawahar A R Adhilakshmi P R Narayanan

Cytokines play an important role in anti-tuberculosis immune response. Skewing of immunity from protective to pathogenic may involve a shift in Th1-Th2 paradigm. Cytokine gene polymorphism is known to be associated with functional differences in cytokine regulation and altered clinical performance in a variety of diseases. The aim of this study was to know whether Interleukin-12B 3' UTR (Taq1) (A/C) and Interleukin-10 (-1082 G/A) gene polymorphisms were associated with susceptibility to pulmonary tuberculosis.IL -10 (-1,082 G/A) and IL-12B gene polymorphisms were studied in 132 pulmonary TB (PTB) patients and 143 normal healthy subjects (NHS), using DNA based polymerase chain reaction (PCR) with sequence specific primers and restriction digestion.The allelic as well as genotypic frequencies of Interleukin -10 (-1082) and Interleukin -12B (3'UTR Taq 1) did not differ significantly between the patients and controls.Our findings suggested that IL -10 (-1082 G/A) and IL -12B 3'UTR (Taq I) (A/C) gene polymorphisms were not associated either with susceptibility or resistance to pulmonary tuberculosis in the south Indian population.

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Is it worth treating category I failure patients with category II regimen

Indian Journal of Tuberculosis (2005)

T Santha P G Gopi R. Rajeswari N Selvakumar R Subramani

Regimen

Sputum culture

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Citations (13)