L. Schaffar

Inserm

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Bernard Ferruà

Inserm

Didier Schmitt

Institut Élie Cartan de Lorraine

Gerald Sonnenfeld

Prisma Health

Serge N. Manié

Centre de Recherche en Cancérologie de Lyon

Irena Konstantinova

NBE Therapeutics (Switzerland)

Bernard Rossi

Beneficência Portuguesa de São Paulo

Breittmayer Jp

Dorothy E. Lewis

The University of Texas Health Science Center at Houston

M. Chavance

Inserm

ANNE KOEBEL

University of Louisville Hospital

Cooperative Institutions

Inserm

Hôpital Pasteur

Centre National de la Recherche Scientifique

Université Côte d'Azur

Hôpital l'Archet

Institut National de la Transfusion Sanguine

University of Louisville

Centre Hospitalier Universitaire de Martinique

Centre Méditerranéen de Médecine Moléculaire

Centre Hospitalier Universitaire de Nice

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In vitro interleukin-1 and 2 production and interleukin 2 receptor expression in the rhesus monkey

Life Sciences (1996)

Didier Schmitt Gerald Sonnenfeld David Husson Jean Tkaczuk Eric André

Rhesus macaque

10.1016/0024-3205(96)00391-8

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Citations (4)

The rhesus monkey as a model for testing the immunological effects of space flight

Advances in Space Research (1994)

Gerald Sonnenfeld L. Schaffar Didier Schmitt Carlos A. Peres E. S. Miller

Cellular immunity

10.1016/0273-1177(94)90430-8

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Citations (6)

Effects of long duration spaceflight on human T lymphocyte and monocyte activity.

PubMed (1991)

Serge N. Manié Irena Konstantinova Breittmayer Jp Bernard Ferruà L. Schaffar

Experiments were performed on blood samples from 5 cosmonauts in order to investigate the effects of long duration spaceflight (26 to 166 days) on immune activity. The experiments were performed on cultured mononuclear cells purified from blood samples collected during the preflight period and 24 h after landing. The production of interleukin 2, which is the major cytokine involved in T lymphocyte proliferation, was found to be enhanced after flight in some individuals, whereas the ability of mitogen-stimulated cells to express interleukin 2 receptor was impaired 24 h after flight for two cosmonauts out of five. Normal interleukin 2 receptor expression was obtained in all cases when lymphocytes were directly activated by a protein kinase C activating phorbol ester. On the other hand, no significant changes were observed in interleukin 1 production by cultured peripheral blood mononuclear cells. Lastly, the distribution of T lymphocytes subsets was examined in peripheral blood sampled 24 h after landing and was found to be within normal values.

Monocyte

Spaceflight

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Detection of human IL-1α and IL-1β at the subpicomolar level by colorimetric sandwich enzyme immunoassay

Journal of Immunological Methods (1988)

Bernard Ferruà Pascale De Becker L. Schaffar Alan Shaw Max Fehlmann

10.1016/0022-1759(88)90151-2

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Citations (43)

Use of Bed Rest and Head-Down Tilt to Simulate Spaceflight-Induced Immune System Changes

Journal of Interferon & Cytokine Research (1996)

Didier Schmitt L. Schaffar G. R. Taylor Karin C. Loftin Victor S. Schneider

Bed rest, both with and without head-down tilt, has been extensively used as an earth-bound analog to study physiologic effects mimicking those occurring in weightlessness during spaceflight. We have been able to show in six subjects that 4 weeks of head-down tilt bed rest induces a significant decrease in interleukin-2 secretion by PHA-stimulated T lymphocytes. Another study, lasting 113 days, with two subjects showed a decreased interleukin-2 receptor expression in PHA-stimulated peripheral blood mononuclear cells but a decreased interleukin-2 production in one subject only. Under the same conditions, interleukin-1 production was largely increased in both subjects. Several other immune parameters were also analyzed. Increased interleukin-1 production could contribute to bone mineral loss encountered during bed rest and decreased interleukin-2 secretion could play a role in the appearance of infectious diseases often observed during bed red.

Spaceflight

Head-Down Tilt

Rest (music)

Weightlessness

10.1089/jir.1996.16.151

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Citations (32)

Fréquence des anticorps anti-HBV, CMV et HTLV chez les donneurs “à risque”

Revue Fran&#xE7 aise de Transfusion et Immuno-h&#xE9 matologie (1984)

A.M. Couroucé Françoise Bouchardeau L. Schaffar

10.1016/s0338-4535(84)80151-8

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Citations (1)

HTLV-I infection in french west indies: A case-control study

European Journal of Epidemiology (1991)

Nadine Fréry M. Chavance Ignez Valette L. Schaffar C. Neisson-Vernant

Martinique

Persistence (discontinuity)

10.1007/bf00237363

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Citations (2)

CD3-stimulated Jurkat T cells mediate IL-1 beta production in monocytic THP-1 cells. Role of LFA-1 molecule and participation of CD69 T cell antigen.

PubMed (1993)

Serge N. Manié Joanna Kubar Martine Limouse Bernard Ferruà Michel Ticchioni

In this study we investigated the T cell signals required for monocyte activation. We used an in vitro co-culture system involving two human cell lines: Jurkat T cells and THP-1 monocytes. Monocyte activation was monitored by measuring IL-1 beta production, whereas IL-2 secretion reflected Jurkat activation. We showed that CD-3 -stimulated Jurkat cells delivered an IL-1-inductive signal to THP-1 cells through a cellular contact which was independent of THP-1 Fc receptors cross-linking. Stimulation of IL-1 beta production did not appear to require lymphokine secretion by T cell since a lymphokine defective mutant of Jurkat cell was able to deliver the stimulatory signal. The LFA-1 molecule was clearly shown to participate in the cooperation process, but its role was likely to be restricted to mediating initial adhesive interaction rather than to transducing the IL-1 -inductive signal. Interestingly, the co-culture stimulated by (Fab')2 fragments of CD3 mAb displayed an enhanced IL-1 beta production without any increase of IL-2 secretion. This result indicated that Jurkat cells could stimulate THP-1 cells even when they were only partially activated. The kinetics and conditions of IL-1 beta production called our attention to the early T cell activation antigen CD69. We then showed that CD69 mAb interfered with transmission of the IL-1 inductive signal (40-50% inhibition of IL-1 production). Our results are suggestive of a new role for CD69 molecule intervening in the T lymphocyte-dependent monocyte activation process.

Jurkat cells

Monocyte

THP1 cell line

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Citations (29)