The influence of human follicular fluid from mature and immature oocytes on the maturation of rabbit oocytes under in-vitro conditions was examined. The human follicular fluids were obtained in the course of laparoscopies for extracorporeal insemination and separated into the categories mature and immature ova. In cases of normal early embryonal development the follicular fluid was termed "follicular fluid of a mature ovum"; if the ova were immature or degenerated the fluid was termed "follicular fluid of an immature ovum". Rabbit oocytes were obtained 3 hours after administration of P-LH and immediately cultivated in 3 different culture media for 7 hours. Only 6 out of 50 rabbit oocytes cultivated in Brackett's Defined Medium with the addition of 20% follicular fluid from an immature human ovum for 7 hours reached prometaphase II to metaphase II. This corresponds to a rate of 12%; on the other hand, with the addition of follicular fluid from a mature ovum 18 out of 50 oocytes reached the same phase, i.e., a rate of 36%. This difference is statistically significant (p less than 0.01). Brackett's Defined Medium with no fluid added was used as a control. Only 5 out of 50 oocytes managed to reach prometaphase II to metaphase II. The rate in this case was 10%. In order to prove that the rabbit oocytes were in the chromatin consolidation phase, they were fixed immediately after being obtained (3 hours after P-LH administration) and stained in 5% Giemsa solution. All of the oocytes (31) were in the chromatin consolidation phase.(ABSTRACT TRUNCATED AT 250 WORDS)

Follicular fluid

Prometaphase

10.1055/s-2008-1036501

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Erste Ergebnisse der Kryokonservierung von menschlichen Oozyten

Geburtshilfe und Frauenheilkunde (1986)

S. Al-Hasani K. Diedrich H. van der Ven D. Krebs

As a part of an IVF project, "superfluous" ova were frozen, then thawed and fertilized under in-vitro conditions. Altogether 133 oocytes of varying quality, from 22 patients, were frozen. To date, 57 ova have been thawed and 47 oocytes have been found. The survival rate after thawing was 34%, the in-vitro fertilization rate 75%. Out of a total of seven embryo transfers performed there was one pregnancy following transfer of a six-cell embryo.

10.1055/s-2008-1036272

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Citations (18)

Kryokonservierung von Kaninchenoozyten im Vorkern-Stadium

Springer eBooks (1989)

C. Hepnar S. Al‐Hasani K. Diedrich H. van der Ven D. Krebs

Stadium

10.1007/978-3-642-74784-7_439

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Testikuläre Spermatozoen für die intracytoplasmatische Spermieninjektion

Springer eBooks (1998)

H. Prieur Markus Montag Kristian Friedrich K. Schirren H. van der Ven

10.1007/978-3-642-71974-5_121

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Vitrification of pronuclear embryos: research basis for aseptic technology and its application to oocytes and blastocysts

CRC Press eBooks (2007)

H. van der Ven Vladimir Isachenko Evgenia Isachenko Markus Montag Frank Nawroth

Development of a refined method for cryopreservation of human pronuclear embryos is an important topic, as it is illegal to cryopreserve an oocyte after fusion of the pronuclei due to ethical difficulties in some countries. To date, in some centers, numerous babies have been born after transfer of embryos developed from frozen pronuclear embryos.1

Vitrification

Aseptic processing

10.3109/9780203090022-18

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WS03‐04Three‐dimentional‐ultrasound in IVF cycles

Ultrasound in Obstetrics and Gynecology (2000)

Ralf L. Schild C Knobloch H. van der Ven M. Hansmann

Aim To investigate the role of 3D‐ultrasound in estimating the likelihood of conception in an IVF programme. Materials and methods We studied a total of 152 women during their stimulation cycle. Apart from conventional 2D‐parameters we assessed endometrial and uterine volume (sub‐)endometrial blood flow (sub‐)endometrial vessel density and ovarian volume by 3D‐ultrasound. Results With regard to subendometrial blood flow on day 1 of the stimulation cycle, all 3D‐indices were significantly lower in the conception vs. non‐conception group. On the day of oocyte retrieval, any difference between the two groups failed to reach statistical significance. The same applied to endometrial volumes. The latter showed a significant corrrelation with uterine size. Ovarian volumes ≤ 3 mm (1 SD below the mean) on day 1 of the stimulation cycle were associated with a nonsignificant lower conception rate. Conclusion Quantitative assessment of spiral artery blood flow and vessel density may be an early predictor of IVF success.

3D ultrasound

Ovarian artery

10.1046/j.1469-0705.2000.00009-1-18.x

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Cellular sensitization against spermatic and seminal plasma antigens in women after intrauterine insemination

Archives of Gynecology and Obstetrics (1990)

W. Schröder Peter Mallmann H. van der Ven K. Diedrich D. Krebs

Heterologous

10.1007/bf02389577

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Citations (5)