ML Gómez-Lus

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Fernando Fuentes

Renown Health

Amelia Serraller Calvo

Universidad Complutense de Madrid

Maestre

Hospital Naval Almirante Nef

Jordi Reina Prieto

Hospital Universitario Son Espases

P Bas

J. Prieto

McMaster University

Francisco Fuentes

University of Córdoba

Fabio Cafini

Universidad Europea de Madrid

Catarina I. V. Ramos

University of Aveiro

Julio Ramón Mairena Gómez

National Autonomous University of Nicaragua

Cooperative Institutions

Universidad Complutense de Madrid

Universitat de Barcelona

Renown Health

University of Nevada, Reno

Consorci Institut D'Investigacions Biomediques August Pi I Sunyer

Hospital Clínic de Barcelona

Alberto Hurtado University

Carilion Clinic

Virginia Tech

Hospital Universitario Miguel Servet

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Activity of oral antibiotics against respiratory tract pathogens in Spain.

PubMed (2003)

Amelia Serraller Calvo R Amores E Valero Fernando Fuentes ML Gómez-Lus

The aim of this study was to carry out a nationwide survey to assess the susceptibility of clinical isolates of four respiratory pathogens against nine antibiotics. Eight Spanish centers participated in the study, collecting a total of 977 isolates of Streptococcus pneumoniae, Streptococcus pyogenes, Haemophilus influenzae and Moraxella catarrhalis. The susceptibility of S. pneumoniae to penicillin was 37.46% susceptible, 30.43% intermediate and 32.11% resistant. MIC(90) of all antibiotics against this microorganism were 48 mg/l, except cefaclor, cefixime and azithromycin. For S. pyogenes, all the strains were susceptible to penicillins and cephalosporins, and azithromycin was the least active with a rate of resistance of 11.43%. A total of 95 isolates of H. influenzae were betalactamase positive (26.32%). With regard to M. catarrhalis, only penicillin and amoxicillin showed MICs(90) >=8 mg/l.

Cefaclor

Cefixime

Streptococcus Pyogenes

Ketolide

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Es la cuantificación del biofilm un elemento diferenciador en la patogenia de bacilos gramnegativos

Revista española de quimioterapia. Suplemento (2013)

Julio Ramón Mairena Gómez ML Gómez-Lus P Bas Catarina I. V. Ramos Fabio Cafini

espanolEl objetivo del estudio fue investigar la formacion de biofilms en bacterias gramnegativas y cuantificar la produccion de biofilm mediante la aplicacion de una tecnica que permitiese una comparacion de los resultados de la formacion de biofilm entre las diferentes especies de gramnegativos. Se estudiaron un total de 153 cepas de bacilos gramnegativos correspondientes a 12 especies bacterianas por el metodo de la densidad optica aplicando una modificacion de la tecnica descrita por Stepanovic et al. Los valores obtenidos mediante el analisis de la densidad optica permiten clasificar a los microorganismos en formadores fuertes, moderados, debiles y no formadores. Los resultados obtenidos se han expresado de dos maneras, ambas utilizando el mismo metodo estadistico: sin estandarizar, donde los controles fueron diferentes dependiendo de los dias en los que se realizaron las medidas; y estandarizados mediante un factor de correccion, utilizando el mismo control para todas las cepas de cada especie, lo que permite su homogeneizacion. Los resultados obtenidos en el estudio tras el analisis y estandarizacion establecen que de las 153 cepas de gramnegativos estudiados, 105 de ellas fueron no formadoras de biofilms, representando el 63,75% de los generos estudiados. Consideramos que la estandarizacion y cuantificacion de la produccion de biofilm entre las bacterias gramnegativas puede resultar de utilidad en el ambito clinico, ya que el conocimiento de la capacidad de produccion de biofilm puede dirigir o enfocar el tratamiento de eleccion de las patologias producidas por dichos microorganismos. EnglishThe aim of the study was to investigate biofilm formation in Gram negative bacteria and to quantify biofilm production applying a new developed technique that made possible to compare results about biofilm formation within the different Gram negative bacteria species. A total of 153 Gram negative strains corresponding to 12 different bacterium species were studied applying a variation of the optic density measurement technique reported by Stepanovic et al. Data obtained with optic density analysis allow to classify microorganisms in strong biofilm developers, moderate biofilm developers, weak biofilm developers and no biofilm developers. The results were expressed in two ways, using in both cases the same statistical method: without standardization, where controls were different depending on the day optic density measurements were performed, and standardized using a correction factor, using the same control for every strain of all our bacterium species in our study, which allows result homogenization. The obtained results in our study after data analysis and standardization show that over the 153 Gram negative strains in our study, 105 of them were no biofilm developers, representing 63.75% of all the studied bacterium genera. We consider that standardization and quantification of biofilm development in Gram negative bacteria can be useful in clinical practice, because biofilm development ability can lead or focus the gold treatment of pathologies produced by these microorganisms

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[Post-antibiotic effect. A factor with clinical applications or simply a laboratory curiosity?].

PubMed (1998)

ML Gómez-Lus Francisco Fuentes José M. Prieto

Curiosity

Factor (programming language)

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