Mohammed H. Awwad

Benha University

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Co-Authors

Sabry El-Serafy

Benha University

Mona Azab

Al Jouf University

Amal M. Abdel-Kareim

Benha University

Amany A. Tohamy

Helwan University

Nahed M. El-Abiad

Egyptian Atomic Energy Authority

Abdel-Monsef A. Elhadary

Egyptian Atomic Energy Authority

Sahar M. Abou El kheir

Zagazig University

Gehan H. Lashien

Zagazig University

Nassr-Allah H. Abdel-Hameid

Benha University

Fatma Ashour

Benha University

Cooperative Institutions

Benha University

Zagazig University

Newcastle University

Egyptian Atomic Energy Authority

Theodor Bilharz Research Institute

The Ohio State University

Institute for Sustainability

Ain Shams University

Centers for Disease Control and Prevention

University of Newcastle Australia

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Genetic investigations on certain genes associated with type 1 diabetes in some Egyptions

Journal of Basic and Environmental Sciences (2024)

Noha A .El kamshishy Safaa Alzawahry Mohammed H. Awwad

10.21608/jbes.2024.391294

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Molecular Identification of Three Argas Species Using Polymerase Chain Reaction (PCR) Amplification and Restriction Analysis of the Small-Subunit Ribosomal RNA Gene

The Egyptian Journal of Hospital Medicine (2002)

Mohammed H. Awwad Sahar M. Abou El kheir Gehan H. Lashien

The polymerase chain reaction (PCR) and restriction fragment length polymorphisms (RFLPs) of SrRNA gene of Argas species (~1800 bp) is a very useful technique for differentiation of Argas persicus, Argas hermanni and Argas arboreus species.The PCR/RFLPs profile of EaeI and EcoRI restriction endonucleases were highly characteristic of the genetic interspecific levels and low genetic intraspecific levels of the three species.Other enzymes proved that A. persicus and A. hermanni may be a single or monophyletic species (SacII and SstII restriction endonucleases).AvaII restriction enzyme showed that A. hermanni and A. arboreus could be a monophyletic species.AvaI restriction endonuclease was the only restriction enzyme to prove that the three Argas species may be polyphyletic species and identified uniquely by this enzyme.

Restriction fragment

10.21608/ejhm.2002.18769

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Molecular Biological Studies on the Effect of the Electromagnetic Fields on ETS-1 Oncogene

The Egyptian Journal of Hospital Medicine (2006)

Amany A. Tohamy Mohammed H. Awwad Nahed M. El-Abiad Abdel-Monsef A. Elhadary

ETS-1 is the founding member of the ETS family of transcription factors. ETS factors have important roles in oncogenesis, signal transduction and development. In human tumors, ETS-1 is expressed in endothelial cells and fibroblasts of the tumor stroma and is proposed to play a role in tumor vascularization and invasion by upregulating expression of matrix- degrading proteases. In human carcinomas, ETS-1 is also expressed by neoplastic cells, but little is known about the functional implications of this observation. The present study aimed to detect the tumor by using electromagnetic fields through ETS-1 oncogene. The detection of point mutations correlated with diseases is currently performed by digestion of PCR products (PCR/RFLP) by using restriction endonucleases. It has been described here a method based modified on primers during the PCR, and using some restriction endonucleases (

10.21608/ejhm.2006.18040

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Mitochondrial NADH Dehydrogenase gene (mtND2) Phylogeny of Egyptian Tilapia species

The Egyptian Journal of Hospital Medicine (2002)

Mohammed H. Awwad

NADH dehydrogenase is a very important protein and is expressed by the mitochondrial NADH dehydrogenase gene (mtND2). Dehydrogenase enzyme is used to remove hydrogen from its substrate, which is used in the cytochrome (hydrogen carrier) system in respiration to produce a net gain of ATP. Also, it reversibly catalyses the oxidation of NADH to NAD and reduced acceptor. The size of mtDN2 of Tilapia species and their hybrids is ~1050 base pairs and was detected by using the polymerase chain reaction technique. To identify the molecular phylogeny and the physical characteristics of mtND2 gene of Tilapia species were done by using the restriction fragment length polymorphisms (RFLPs) with some restriction endonucleases(AccI, AvaII, AvaI, StyI, Bg1I and EaeI). The PCR-RFLPs of NADH dehydrogenase gene of Tilapia species and their hybrids may prove that the gene is quite evolution phylogenetic difference from one species to another. At the same time, This study investigated the feasibility of mitochondrial DNA (mtDNA) based approaches in addressing problems of identification of Tilapia species and their hybrids, isolated from the River Nile by using the PCR-RFLPs analysis of mtND2 gene.

NADH dehydrogenase

10.12816/ejhm.2002.18853

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Identification of Athletic Human Growth Hormone Gene Polymorphisms

The Egyptian Journal of Hospital Medicine (2007)

Mohammed H. Awwad Hany A. Eldeeb

I restriction endonucleases gave high levels of intraspecific profile for all the studied gene of the ten athletes. Other enzymes including

Identification

Human growth hormone

10.21608/ejhm.2007.17712

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Estrogen receptor positive breast tumors resist chemotherapy by the overexpression of P53 in Cancer Stem Cells

Journal of the Egyptian National Cancer Institute (2018)

Fatma Ashour Mohammed H. Awwad Hayam E.L. Sharawy Mohamed Kamal

Breast cancer (BC) is classified according to estrogen receptor (ER) status into ER+ and ER− tumors. ER+ tumors have a worse response to chemotherapy compared to ER− tumors. BCL-2, TP53, BAX and NF-ΚB are involved in drug resistance in the ER+ tumors. Recently it was shown that Cancer Stem Cells (CSCs) play an important role in drug resistance. In this study we tested the hypothesis that CSCs of the ER+ tumors resist drug through the overexpression of BCL-2, TP53, BAX and NF-ΚB. CSCs were isolated by anoikis resistance assay from MCF7 (ER+) and MDA-MB-231 (ER−) cell lines. Isolated CSCs were treated with doxorubicin (DOX) and the mRNA expression levels of BCL-2, TP53, BAX and NFKB were investigated by quantitative real time PCR (qPCR) with and without treatment. BCL-2, BAX and NF-ΚB showed decreased expression in MCF7 bulk cancer cells after DOX treatment whereas only BCL-2 and BAX showed decreased expression in MDA-MB-231 bulk cancer cells. Interestingly TP53 was the only gene showed a considerable increase in its expression in CSCs of the ER+ MCF7 cell line compared to bulk cancer cells. Moreover, TP53 was the only gene showing exceptionally higher level of expression in MCF7-CSCs compared to MDA-MB-231-CSCs. Our results suggest that CSCs in the ER+ cells escape the effect of DOX treatment by the elevation of p53 expression.

10.1016/j.jnci.2018.04.002

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Using Restriction-Site Variation of PCR-Amplified 18S Ribosomal RNA Gene for Phylogenetic Analysis of Hymenolepis spp.

The Egyptian Journal of Hospital Medicine (2001)

Mohammed H. Awwad Gehan H. Lashien Sahar M. Abou El kheir

Hymenolepis spp.infections are often asymptomatic, especially in light cases.Heavy infections can induce enteritis with nausea and vomiting, diarrhea, abdominal pain, and dizziness.Genetic therapy is the most future promising trend for treatment and prevention so, a genotype map of different parasite on microorganisms must be done.A simple and rapid polymerase chain reaction/restriction fragment length polymorphisms (PCR/RFLPs) assay, using the common restriction endonucleases HindIII, Bg1I, EcoRI, BanII, SacII and SstII, is described to illustrate the genetic structure of both Hymenolepis species.All restriction endonucleases have been used to differentiate between both species and based on ~2200 bp long sequence of the 18S ribosomal RNA gene.H. nana and H. diminuta were undifferentiated when their 18S rRNA genes digested with HindIII restriction endonuclease.The two Hymenolepis were welldifferentiated when their 18S ribosomal RNA genes were digested with Bg1I and EcoRI restriction endonucleases.It's clear observed that BanII, SacII and SstII restriction enzymes could be used as a genetic marker for H. nana when the enzymes uniquely fragmented the 18S rRNA gene without digesting the gene of H. diminuta.

Restriction site

10.21608/ejhm.2001.18861

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DNA riboprinting analysis of Tilapia species and their hybrids using restriction fragment length polymorphisms of the small subunit ribosomal DNA

Aquaculture Research (2007)

Sabry El-Serafy Nassr-Allah H. Abdel-Hameid Mohammed H. Awwad Mona Azab

Morphometric, meristic and DNA riboprinting analyses of Tilapia species and their hybrids inhabiting the River Nile were examined. Morphometric data showed striking similarities and overlapping among Tilapia species, making it impossible to differentiate these species. Meristic characteristics revealed that Tilapia species could be identified into four major groups (Oreochromis niloticus, O. aureus, Sarotherodon galilaeus and Tilapia zillii). The lateral line scales differed significantly between the four Tilapia species, while the number of fin rays in the dorsal and anal fins differed significantly, differentiating three species (but not between O. niloticus and O. aureus). Restriction fragment length polymorphisms (RFLPs) of nuclear small sub-unit ribosomal RNA (18S srRNA) gene were used to differentiate the species. Polymerase chain reaction-restriction fragment length polymorphisms data provided a unique pattern for each species with a specific restriction enzyme. Two hybrids of Tilapia designated H1 and H2 were detected. The endonucleases SacII and ApaI differentiated H1 and H2. This research revealed a monophylogenetic relationship among all the studied Tilapia species.

Meristics

Nile tilapia

Restriction fragment

10.1111/j.1365-2109.2007.01668.x

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Citations (10)

Molecular diagnosis of Type I diabetes in some Egyptian patients using HLA-DRB1 exon 2 gene

Benha Journal of Applied Sciences (2022)

Kholud Z.Higazy Amal M. Abdel-Kareim Naglaa F. Alhusseini Mohammed H. Awwad

Type 1 diabetes is regarded as a chronic autoimmune disease characterized by dysfunctional Tlymphocyte activation that targets pancreatic Langerhans β-cells, causing insulin deficiency.By raising the amounts of sugar in the blood and urine, this condition causes hyperglycemia.Five groups were chosen for the current experiment, with N standing in for healthy individuals.Four groups of patients were created based on their ages.The bidirectional sequence of the HLA-DRB1 exon 2 gene was determined using blood samples from the five groups.PCR was used to define the gene sequence, amplify it, and isolate the gene for DNA sequencing in two directions.To understand more about the isolated gene and its role in T1DM, bioinformatics analysis of the sequencing output data was carried out.In addition, certain software programs were used by the study teams to forecast the translated protein and RNA secondary structure.The findings showed that an alteration in the secondary structure of RNA affected protein translation and release in bodily cells.Additionally, based on SNP analysis, the four groups' targeted genes revealed mutations caused by the replacement or indel of certain nucleotides, which change the anticipated translated protein in patient groups in comparison to control.

HLA-DRB1

10.21608/bjas.2022.308845

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Combretastatin A-4 disodium phosphate and low dose gamma irradiation suppress hepatocellular carcinoma by downregulating ROCK1 and VEGF gene expression

Molecular Biology Reports (2020)

Zakaria Mohamed Alghzzawy Tarek Khaled Elmaghraby Sanaa A. Hagag Mohammed H. Awwad

Combretastatin

CD31

Immunostaining

10.1007/s11033-020-05282-0

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Citations (4)