Abstract To develop a more dependable method of diagnosing hepatitis C, serum anti‐hepatitis C virus (HCV) was examined by using a new assay (anti‐HCV second generation). The results were compared with those of either the conventional assay (anti‐HCV first generation) or HCV‐RNA analysis. With the first generation assay, anti‐HCV was detected in 69% of post‐transfusion acute hepatitis (AH), 44% of sporadic AH, 50% of needlestick exposed AH, 72% of chronic hepatitis (CH), 77% of liver cirrhosis (LC) and 86% of hepatocellular carcinoma (HCC). These results were remarkably increased by using the second generation assay (92% in post‐transfusion AH, 72% in sporadic AH, 100% in needlestick exposed AH, 96% in CH, 96% in LC and 97% in HCC). Furthermore, in the early stages of AH (from 1–5 weeks after onset), anti‐HCV was not detected in all 18 patients by the first generation assay, but was found in 10 of them by using the second generation assay. The failure to detect anti‐HCV with the first generation assay was mainly due to a lack of the core region coding peptide (C22‐3) in this assay. In the AH‐resolving group, anti‐HCV second generation did not disappear, but the titre tended to be lower than that in the CH‐developing group. Thus, the second generation assay for anti‐HCV was considered to be a more useful tool for not only the diagnosis of hepatitis C but also for determining prognosis.

Hepatitis C

10.1111/j.1440-1746.1992.tb01493.x

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Immunohistochemical detection of proliferating cell nuclear antigen in hepatocellular carcinoma: Relationship to histological grade

Journal of Gastroenterology and Hepatology (1993)

Makiko Taniai M Tomimatsu Hiroyuki Okuda Akiko Saito Hiroshi Obata

Proliferating cell nuclear antigen (PCNA), also known as cyclin, is an auxiliary protein of DNA polymerase-delta and is found only in the nuclei of proliferating cells in the late G1 and S phases. The proliferation of hepatocellular carcinoma (HCC) by immunohistochemical staining for PCNA using paraffin sections of 20 surgically resected HCC specimens was analysed. The mean percentage of PCNA-positive nuclei in the HCC tissue was 10.3% in grade I of Edmondson and Steiner's classification, 25.5% in grade II, 28.4% in grade III and 41.5% in grade IV. In early HCC, we observed only a few PCNA-positive tumour cells. However, PCNA-positive nuclei were numerous in the tumour thrombi found in portal vein branches, in regions of extracapsular tumour growth, and in the inner nodules of tumours with a nodule-in-nodule formation. Proliferating cell nuclear antigen positivity was correlated with an increase of the nucleocytoplasmic ratio of tumour cells as determined by image analysis. Our findings showed that PCNA positivity was correlated with the histological grade and invasiveness of HCC, suggesting that this antigen may be used as an indicator to predict tumour invasion in patients with HCC.

10.1111/j.1440-1746.1993.tb01541.x

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Citations (15)

325. Usefulness of CT-AP by simultaneous catheterization into SMA and SpA with HCC

Japanese Journal of Radiological Technology (1993)

Mitsuru Hashiguchi Y. Hashiguchi Norimichi Saitoh I Uchida M Tomimatsu

10.6009/jjrt.kj00003324912

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Clinical Features of Elderly Patients with Hepatocellular Carcinoma.

Nippon Ronen Igakkai Zasshi Japanese Journal of Geriatrics (2001)

Hideki Endo M Tomimatsu Shin-ichiro Ohkawa

高齢者肝細胞癌 (HCC) 症例の臨床的特徴と治療法選択に関し, 自験例163例を older (70歳以上; O群47例), intermediate (60歳以上70歳未満; IM群70例) 及び younger (60歳未満; Y群46例) の三群に分け検討した.O群ではY群に比し HBsAg 陽性例とIII期症例が少なかった (p<0.05). また, 飲酒歴を有するものの割合も有意に低率であった (p=0.006). 性比では女性が, 腫瘍のサイズでは small HCCの割合がO群で多い傾向はみられたものの, 有意差はなかった. 輸血からHCC診断までの期間は各群とも約30年で差はなかった. 治療はO群76%, IM群87%, Y群78%で行えたが, 外科切除例はO群では2例と少なかった. 他病の有病率はO群53%, IM群30%, Y群28%とO群で有意に高く, 特に心肺疾患が多くみられた. 無治療例の理由はIM群, Y群とも全て肝臓側の要因であったが, O群では治療拒否や心不全なども見られた. 5年生存率はO群55%, IM群32%, Y群26%でO群が他群より高く (p<0.01), 特に他病の合併のないO群症例は予後良好であった.以上より, 高齢者HCCは, 腫瘍サイズや肝予備能からみれば侵襲のある積極的治療も可能であるが, QOLの面や他病の有病率が高いことから, より低侵襲な治療法を選択すべきであると考えられた. 更に, 心肺疾患のコントロールが十分出来れば, むしろ非高齢者HCCより予後が良い可能性が示唆された.

Group A

Concomitant

Group B

10.3143/geriatrics.38.791

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Citations (1)

A case of hepatic hydrothorax who was diagnosed by intraperitoneal indocyanine green (ICG) injection and recovered through chemical pleurodesis

Tokyo Joshi Ika Daigaku zasshi/Tokyo Joshi Ika Daigaku Zasshi (1992)

Takayuki Fujino M Tomimatsu Kouko Chishima Mitsuaki Fukuyo Norihiko Shimoyama

Indocyanine Green

Pleurodesis

Source

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Citations (1)

[A case of hepatocellular carcinoma which developed from type B hepatitis and presented marked hypoglycemia].

PubMed (1987)

T Katoh Soyun Cho M Tomimatsu Etsuko Hashimoto T Hisamitsu

Source

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Prognostic significance of MCM2high/Ki-67high in ameloblastoma

Journal of Oral and Maxillofacial Surgery Medicine and Pathology (2023)

Michiyo Ando Satoru Miyabe S. Okubo Atsushi Nakayama M Tomimatsu

Clinical Significance

Ki-67

10.1016/j.ajoms.2023.10.011

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Levels of telomerase catalytic subunit mRNA as a predictor of potential malignancy.

International Journal of Oncology (1999)

Hisashi Hisatomi Kumi Nagao T Kanamaru Hitoshi Endo M Tomimatsu

This study investigated the relationship between the levels of human telomerase reverse transcriptase (hTERT) mRNA and that of telomerase activity in hepatocellular carcinoma (HCC). A significant correlation between hTERT mRNA expression and telomerase activity by transfecting the gene encoding hTERT into telomerase-negative human fibroblast cells has clearly been demonstrated. However, the relationship between levels of telomerase activity and that of hTERT mRNA has yet to be elucidated. In this study, the levels of hTERT mRNA were analyzed in 24 HCC patients by real-time PCR. And the intensity of telomerase activity was analyzed by fluorescence-based TRAP method. The difference of hTERT mRNA level was highly significant between tumor tissues and non-cancerous liver tissues. And there were significant correlations between the levels of hTERT mRNA and that of telomerase activity (r=0.751) in tumor tissues. We observed a strong correlation between levels of hTERT mRNA and that of telomerase activity in HCC. Our results suggest that the levels of hTERT mRNA would be useful in genetic diagnostic tests, instead of telomerase activity, to screen at-risk patients of HCC in human liver tissues.

10.3892/ijo.14.4.727

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Citations (47)