V. K. Ghosh

University at Buffalo, State University of New York

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Ramaswamy C. Anantheswaran

Pennsylvania State University

J. K. Deb

Aditya Birla (India)

Gregory R. Ziegler

Pennsylvania State University

Isaac Ang

University of Illinois Urbana-Champaign

Shriramprasad Venkatesan

University at Buffalo, State University of New York

Rudiyanto Gunawan

UNSW Sydney

Changyou Chen

Institute of Electrical Engineering

Sriram Neelamegham

University at Buffalo, State University of New York

Panagiotis Chrysinas

University at Buffalo, State University of New York

Qiaoping Yuan

National Institutes of Health

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University at Buffalo, State University of New York

Indian Institute of Technology Delhi

Pennsylvania State University

University of Illinois Urbana-Champaign

North Carolina State University

State University of New York

National Institutes of Health

National Institute on Alcohol Abuse and Alcoholism

The 309th Hospital of Chinese People's Liberation Army

Hunan Provincial People's Hospital

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Production and characterization of xylanase from Thielaviopsis basicola

Applied Microbiology and Biotechnology (1988)

V. K. Ghosh J. K. Deb

Cellulosic ethanol

10.1007/bf00258349

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Citations (16)

Cell- and tissue-specific glycosylation pathways informed by single-cell transcriptomics

NAR Genomics and Bioinformatics (2024)

Panagiotis Chrysinas Shriramprasad Venkatesan Isaac Ang V. K. Ghosh Changyou Chen

Abstract While single-cell studies have made significant impacts in various subfields of biology, they lag in the Glycosciences. To address this gap, we analyzed single-cell glycogene expressions in the Tabula Sapiens dataset of human tissues and cell types using a recent glycosylation-specific gene ontology (GlycoEnzOnto). At the median sequencing (count) depth, ∼40–50 out of 400 glycogenes were detected in individual cells. Upon increasing the sequencing depth, the number of detectable glycogenes saturates at ∼200 glycogenes, suggesting that the average human cell expresses about half of the glycogene repertoire. Hierarchies in glycogene and glycopathway expressions emerged from our analysis: nucleotide-sugar synthesis and transport exhibited the highest gene expressions, followed by genes for core enzymes, glycan modification and extensions, and finally terminal modifications. Interestingly, the same cell types showed variable glycopathway expressions based on their organ or tissue origin, suggesting nuanced cell- and tissue-specific glycosylation patterns. Probing deeper into the transcription factors (TFs) of glycogenes, we identified distinct groupings of TFs controlling different aspects of glycosylation: core biosynthesis, terminal modifications, etc. We present webtools to explore the interconnections across glycogenes, glycopathways and TFs regulating glycosylation in human cell/tissue types. Overall, the study presents an overview of glycosylation across multiple human organ systems.

Nucleotide sugar

Cell type

Glycome

10.1093/nargab/lqae169

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Improvement of T. reesei strain through mutation and selective screening techniques

Biotechnology and Bioengineering (1982)

V. K. Ghosh T. K. Ghose K. S. Gopalkrishnan

New delhi

10.1002/bit.260240120

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Citations (41)

Production of thermostable acid protease by Aspergillus niger

Letters in Applied Microbiology (1994)

Amrendra Kumar singh V. K. Ghosh Priyanka Ghosh

Aspergillus niger F2078 produces high levels of extracellular thermostable acid protease within 96 h. Although glucose and peptone were the best carbon and nitrogen sources, respectively, sucrose and a cheap nitrogen source, corn steep liquor, also gave satisfactory enzyme yields. Supplementation of groundnut meal to the basal medium enhanced enzyme production. Temperature and pH optima of the enzyme activity were 60°C and 3.0–4.0, respectively. The enzyme was stable between pH 3.0 and 6.0 and at temperatures up to 60°C.

Aspergillus niger

Corn steep liquor

10.1111/j.1472-765x.1994.tb00839.x

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Citations (22)

Moisture migration through chocolate-flavored confectionery coatings

Journal of Food Engineering (2004)

V. K. Ghosh Gregory R. Ziegler Ramaswamy C. Anantheswaran

Water activity

10.1016/j.jfoodeng.2004.03.012

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Citations (44)

Respiration rate of fresh button mushrooms, Agaricus bisporus

Mushroom Research (2005)

Qiaoping Yuan V. K. Ghosh Ramaswamy C. Anantheswaran

Agaricus bisporus

Agaricus

Respiration rate

Source

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Production and characterization of xylanase from

V. K. Ghosh J. K. Deb

Summary. The black rot fungus Thielaviopsis basicola has the ability to grow on cellulosic biomass, producing xylanase. Of the four cellulosic substrates tested, rice straw was found to be the best for production of xylanase. A xylanase activity of 34 U/ml was obtained with rice straw which was more than three times that obtained with larchwood xylan. The fl-xylosidase activities obtained with these two substrates were 0.05 U/ml and 0.016 U/ml respectively. Both enzymes are active at pH 5 but the temperature optima of xylanase and fl-xylosidase activities are 60°C and 40°C respectively. The xylanase activity is stable over a pH range of 4--8 but the stability towards temperature falls sharply above 50 ° C.

Cellulosic ethanol

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Fat, Moisture, and Ethanol Migration through Chocolates and Confectionary Coatings

Critical Reviews in Food Science and Nutrition (2002)

V. K. Ghosh Gregory R. Ziegler Ramaswamy C. Anantheswaran

Abstract The migration of fat, moisture, and ethanol is a common problem with chocolate-coated confectionery products. Migration of one of these components into the coating leads to visual and sensory defects such as sugar or fat bloom, making the product unacceptable to the consumer. The migration rate depends on the structure and composition of the coating. The migration of each of these species can be slowed to a certain extent by proper tempering of the coating, because proper tempering will give a structure that resists migration. In the continuous lipid phase, these chemical species migrate mainly through the liquid portion. Thus, the migration rate depends on the amount of liquid oil present in the product. Migration can be delayed either by reducing the liquid fat content or by immobilizing the liquid phase. The actual mechanisms for the migration processes are speculative, and a more thorough understanding is necessary to better abate quality deterioration. Armed with this understanding, a manufacturer would know a priori the effect of changing the ingredient or process. A few methods for control have been suggested, but have found limited application. Mathematical models have been proposed to predict the migration behavior, but their application is hindered because of the simplified assumptions employed. There is a need for developing better models that combine mass transfer with the phase behavior to be able to accurately predict the migration process. This review discusses the current understanding of fat, moisture, and ethanol migration through chocolate coatings and also includes a brief description of the theoretical aspects governing migration. Keywords: diffusionfat bloomsugar bloom.

Tempering

Ingredient

10.1080/20024091054265

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Citations (124)

Cell and tissue-specific glycosylation pathways informed by single-cell transcriptomics

bioRxiv (Cold Spring Harbor Laboratory) (2023)

Panagiotis Chrysinas Shriramprasad Venkatesan Isaac Ang V. K. Ghosh Changyou Chen

While single cell studies have made significant impacts in various subfields of biology, they lag in the Glycosciences. To address this gap, we analyzed single-cell glycogene expressions in the Tabula Sapiens dataset of human tissues and cell types using a recent glycosylation-specific gene ontology (GlycoEnzOnto). At the median sequencing (count) depth, ~40-50 out of 400 glycogenes were detected in individual cells. Upon increasing the sequencing depth, the number of detectable glycogenes saturates at ~200 glycogenes, suggesting that the average human cell expresses about half of the glycogene repertoire. Hierarchies in glycogene and glycopathway expressions emerged from our analysis: nucleotide-sugar synthesis and transport exhibited the highest gene expressions, followed by genes for core enzymes, glycan modification and extensions, and finally terminal modifications. Interestingly, the same cell types showed variable glycopathway expressions based on their organ or tissue origin, suggesting nuanced cell- and tissue-specific glycosylation patterns. Probing deeper into the transcription factors (TFs) of glycogenes, we identified distinct groupings of TFs controlling different aspects of glycosylation: core biosynthesis, terminal modifications, etc. We present webtools to explore the interconnections across glycogenes, glycopathways, and TFs regulating glycosylation in human cell/tissue types. Overall, the study presents an overview of glycosylation across multiple human organ systems.

Nucleotide sugar

Cell type

10.1101/2023.09.26.559616

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Citations (2)

OXYGEN TRANSMISSION RATE THROUGH MICRO‐PERFORATED FILMS: MEASUREMENT AND MODEL COMPARISON

Journal of Food Process Engineering (2001)

V. K. Ghosh Ramaswamy C. Anantheswaran

ABSTRACT Static and flow‐through techniques were used to experimentally measure the oxygen transmission rate of micro‐perforated films. The static method simulates the actual package conditions but is very time consuming. Whereas, the flow‐through method is relatively simple and takes less time to give the results, but it gave higher values than that obtained by the static method. A regression equation was developed to correlate the data obtained by these two methods. Published models for predicting gas exchange through micro‐perforations were evaluated. The predicted data by these models were compared with the experimental data obtained by the static method and the flow‐through method for six different films. The model proposed by Fishman et al. (1996) (J=‐D(c‐c A )/L h ; L h = thickness of the film + radius of the perforation) had very good agreement with the experimental data from the static method.

Perforation

10.1111/j.1745-4530.2001.tb00535.x

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Citations (43)