BACKGROUND: Patients with tracheotomy are often monitored in the anesthesia recovery room after reoperation. During this period, oxygen therapy is necessary, and the existing tracheostomy oxygen supply device has many defects. OBJECTIVE: To evaluate the efficacy of a self-made tracheostomy oxygen delivery device on oxygen therapy during postoperative anesthesia recovery. METHODS: Patients were randomly divided into two groups, E and C, with 30 patients in each group, and admitted to the post-anesthesia care unit (PACU). Patients in group E received oxygen through a self-made tracheostomy oxygen delivery device, while patients in group C were supplied oxygen through a unilateral nasal cannula. Respiration (R), pulse oximetry (SpO2), and the number of patients on ventilators were recorded at the time of admission (T0) and one hour after admission (T1). Rapid dry blood gas analyses were performed on 0.6 ml samples of arterial blood collected at T0 and T1. RESULTS: Compared to group C, patients in group E had significantly higher arterial partial pressure of oxygen (PaO2), arterial oxygen saturation (SaO2), total carbon dioxide (T-CO2), and actual bicarbonate (AB), while arterial partial pressure of carbon dioxide (PaCO2) was significantly reduced (P< 0.01 or < 0.05). Compared to T0, PaO2 decreased in both groups at T1, PaCO2 decreased in group E, while SaO2, T-CO2, and AB decreased in group C (P< 0.01 or < 0.05). CONCLUSION: We found that using the self-made tracheostomy oxygen delivery device in postoperative anesthesia recovery had advantages such as a secure connection to the tracheostoma, adjustable oxygen concentration, air filtration, and the ability to switch oxygen supply between the ventilator and humidifier.
Ethnopharmacological relevance: Siwu paste (SWP) is a classic prescription from the 'Xian Shou Li Shang Xu Duan Secret Recipe', which usually has been used for nourishing blood or blood deficiency in traditional Chinese medicine with over 1000 years. Yet, the SWP’s hematopoiesis mechanism is still unclear.Aim of the study: To investigate the regulation mechanism of hematopoiesis of SWP in anemia rats.Materials and methods: Blood cell and biochemical analysis were used to evaluate the hematopoietic function of SWP in anemia rats. The intestinal microbial composition and the metabolites was analysed with 16S rRNA gene sequencing and UPLC-TripleTOF system nontargeting metabolomics, respectively. The correlation digging was applied to construct the interaction between gut microbiota and blood hematopoiesis, and to explain the potential role of gut microbiota for the hematopoiesis of SWP in anemia patients.Results: Our study found that SWP can improve the levels of red blood cell, hemoglobin, platelet, hematocrit value, white blood cell, lymphocyte, EPO, TPO, and GM-CSF in anemia rats, and significantly change the microbial community and its metabolites. The correlation analysis of intestinal microbiota-hematopoietic efficacy show that 13 kinds of different intestinal flora were related to hematopoietic efficacy, in which Prevotella_1, Prevotella_9, Lactobacillus and norank_f__Muribaculaceae were significantly positively correlated with hematopoiesis, nine kinds of intestinal flora are negative correlated with hematopoietic effect (e.g., Roseburia, Allobaculum, unclassified_f__Ruminococcaceae, Romboutsia). Compared with anemia rats, 218 potential metabolic biomarkers and 36 metabolites with significant differences were identified in SWP treatment group, and the key metabolites were mainly about amino acids and lipids. In-depth analysis of metabolic pathways showed that SWP mainly affected 7 metabolic pathways, which including aminobenzoic acid degradation and tryptophan metabolism.Conclusion: In this study, our results provide the novel insights into the regulation of hematopoiesis of SWP on anemia rats were correlated with gut microbiota and their metabolites, which through the restoration of the firmicutes/bacteroidetes ratio (e.g., norank_f_Muribaculaceae, Lactobacillus and Prevotella_9).
Purpose Early T-cell precursor acute lymphoblastic leukemia (EPT-ALL) is a rare type of ALL that shows genetic characteristics of both ALL and acute myeloid leukemia (AML) but has a poorer prognosis and a higher recurrence rate. The aims of this study were to explore the underlying molecular mechanisms and specific biomarkers of EPT-ALL by bioinformatics analysis. Methods Two expression profile datasets (GSE8879 and GSE28703) were integrated to identify candidate genes of EPT-ALL. Differentially expressed genes (DEGs) between EPT-ALL and classic T-ALL were identified using edge R package. Protein–protein interaction (PPI) network clustering modules were analyzed with STRING and Cytoscape. In addition, the plugins of Cytoscape was used for hub gene functional enrichment analysis, the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway and PPI network analysis. Survival analysis was performed by GEPIA and CCLE database. Results The GSE profiles shared 132 DEGs, including 63 upregulated genes and 69 downregulated genes. KEGG enrichment analysis of DEGs showed that the top 3 pathways were hematopoietic cell lineage, NF-kappaB signaling pathway and T cell receptor signaling pathway. Twenty-seven hub genes were identified from PPI by Cytoscape. High expression of ITGAM (integrin subunit alpha M) and LYN (LYN proto-oncogene) were associated with statistical significantly poorer overall survival rates by survival analysis using GEPIA database. Besides, LYN was differently expressed in EPT-ALL compared with classic T-ALL by searching in CCLE database. Conclusion We screened two hub genes which may consider as candidate novel biomarker in EPT. In summary, we elucidated LYN as a biomarker for the differentiation of EPT-ALL from classic T-ALL.
To analyze the risk factors of primary poor graft function (PGF) after allogeneic hematopoietic stem cell transplantation (allo-HSCT) in patients with myeloid malignancies and the impact of primary PGF on survival.
To analyze the risk factors for late-onset hemorrhagic cystitis (LOHC) after allogeneic hematopoietic stem cell transplantation (allo-HSCT), the risk factors for the progression of LOHC to severe LOHC, and the effect of LOHC on survival.
Abstract We aimed to investigate outcomes of different post-remission treatment (PRT) choices based on dynamic measurable residual disease (MRD) by multiparameter flow cytometry in favorable-risk AML (FR-AML). Four hundred and three younger patients with FR-AML in first complete remission (CR1) were enrolled in this registry-based cohort study, including 173 who received chemotherapy (CMT), 92 autologous stem cell transplantation (auto-SCT), and 138 allogeneic SCT (allo-SCT). The primary endpoint was the 5-year overall survival (OS). Subgroup analyses were performed based on dynamic MRD after the 1st, 2nd, and 3rd courses of chemotherapy. In subgroups of patients with negative MRD after 1 or 2 course of chemotherapy, comparable OS was observed among the CMT, auto-SCT, and allo-SCT groups ( p = 0.340; p = 0.627, respectively). But CMT and auto-SCT had better graft-versus-host-disease-free, relapse-free survival (GRFS) than allo-SCT in both subgroups. For patients with negative MRD after three courses of chemotherapy, allo-SCT had better disease-free-survival than CMT ( p = 0.009). However, OS was comparable among the three groups ( p = 0.656). For patients with persistently positive MRD after 3 courses of chemotherapy or recurrent MRD, allo-SCT had better OS than CMT and auto-SCT ( p = 0.011; p = 0.029, respectively). Dynamic MRD might improve therapy stratification and optimize PRT selection for FR-AML in CR1.
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