Ana Teresa Viana

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In silico prediction of the enzymes involved in the degradation of the herbicide molinate by Gulosibacter molinativorax ON4T

Scientific Reports (2022)

Ana R. Lopes Egor S. Bunin Ana Teresa Viana Hugo J.C. Froufe Antonio Muñoz‐Mérida

Abstract Gulosibacter molinativorax ON4 T is the only known organism to produce molinate hydrolase (MolA), which catalyses the breakdown of the thiocarbamate herbicide into azepane-1-carboxylic acid (ACA) and ethanethiol. A combined genomic and transcriptomic strategy was used to fully characterize the strain ON4 T genome, particularly the mol A genetic environment, to identify the potential genes encoding ACA degradation enzymes. Genomic data revealed that mol A is the only catabolic gene of a novel composite transposon (Tn 6311 ), located in a novel low copy number plasmid (pARLON1) harbouring a putative T4SS of the class FATA. pARLON1 had an ANI value of 88.2% with contig 18 from Agrococcus casei LMG 22410 T draft genome. Such results suggest that pARLON1 is related to genomic elements of other Actinobacteria , although Tn 6311 was observed only in strain ON4 T . Furthermore, genomic and transcriptomic data demonstrated that the genes involved in ACA degradation are chromosomal. Based on their overexpression when growing in the presence of molinate, the enzymes potentially involved in the heterocyclic ring breakdown were predicted. Among these, the activity of a protein related to caprolactone hydrolase was demonstrated using heterologous expression. However, further studies are needed to confirm the role of the other putative enzymes.

Degradation

10.1038/s41598-022-18732-5

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Citations (3)

Carbon-based materials for water disinfection and heavy metals removal

Environmental Technology (2022)

José R.M. Barbosa A. Sofia G.G. Santos Ana Teresa Viana Alexandra G. Gonçalves Olga C. Nunes

The presence of heavy metals and/or harmful bacteria in drinking water represents significant risks to human health. This study aimed to develop a low-cost water treatment technology using synthesized nanocomposites with metal nanoparticles supported on activated carbon (AC) for bacteria and heavy metal removal. In addition, the performance of the developed nanomaterials was compared with that of commercial materials – carbon fibers of three different typologies. The chemical and textural properties of all tested materials were characterized. To simulate a technology to be applied in a water outlet point, removal tests were carried out in a continuous system using suspensions of Escherichia coli and/or Staphylococcus aureus, wherein the contact time with the two phases was minimal (1 min). The obtained results revealed that iron and copper oxides supported on AC with a calcination treatment (CuFeO/AC-C) was the nanocomposite with the best performance, achieving a 6 log reduction for both bacteria in the same suspension up to 9 h operation. A mix of bacteria and heavy metals, simulating a real water, was treated with CuFeO/AC-C obtaining a 6 log reduction of bacteria, a Pb2+ removal >99.9% and Cd2+ removal between 97 and 98% over 180 passage times.

Carbon fibers

Nanomaterials

Suspension

Portable water purification

10.1080/09593330.2022.2154173

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Citations (4)

Environmental superbugs: The case study of Pedobacter spp.

Environmental Pollution (2018)

Ana Teresa Viana Tânia Caetano Claúdia Covas Tiago Santos Sónia Mendo

10.1016/j.envpol.2018.06.047

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