Wei-Ke Jiang

Guizhou University

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Chenghong Xiao

Guizhou University

Lanping Guo

Chinese Academy of Medical Sciences & Peking Union Medical College

Tao Zhou

Guizhou University

Qing‐Song Yuan

Jiangsu University of Science and Technology

Changgui Yang

Guizhou University

Xiaohong Ou

Guiyang College of Traditional Chinese Medicine

Chuan‐Zhi Kang

Chinese Academy of Medical Sciences & Peking Union Medical College

Tao Zhou

Guizhou University

Jinqiang Zhang

Guizhou University

Tao Zhou

Guiyang College of Traditional Chinese Medicine

Cooperative Institutions

Guizhou University

Guiyang College of Traditional Chinese Medicine

Chinese Academy of Medical Sciences & Peking Union Medical College

Chinese Academy of Sciences

Ministry of Agriculture and Rural Affairs

China Academy of Chinese Medical Sciences

Guiyang University

Central South University

Sun Yat-sen University

University of Electronic Science and Technology of China

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De Novo Sequencing and Assembly Analysis of the Pseudostellaria heterophylla Transcriptome

PLoS ONE (2016)

Jun Li Zhen Wei Long Dengkai Ling Ding Anhui Gong

Pseudostellaria heterophylla (Miq.) Pax is a mild tonic herb widely cultivated in the Southern part of China. The tuberous roots of P. heterophylla accumulate high levels of secondary metabolism products of medicinal value such as saponins, flavonoids, and isoquinoline alkaloids. Despite numerous studies on the pharmacological importance and purification of these compounds in P. heterophylla, their biosynthesis is not well understood. In the present study, we used Illumina HiSeq 4000 sequencing platform to sequence the RNA from flowers, leaves, stem, root cortex and xylem tissues of P. heterophylla. We obtained 616,413,316 clean reads that we assembled into 127, 334 unique sequences with an N50 length of 951 bp. Among these unigenes, 53,184 unigenes (41.76%) were annotated in a public database and 39, 795 unigenes were assigned to 356 KEGG pathways; 23,714 unigenes (8.82%) had high homology with the genes from Beta vulgaris. We discovered 32, 095 DEGs in different tissues and performed GO and KEGG enrichment analysis. The most enriched KEGG pathway of secondary metabolism showed up-regulated expression in tuberous roots as compared with the ground parts of P. heterophylla. Moreover, we identified 72 candidate genes involved in triterpenoids saponins biosynthesis in P. heterophylla. The expression profiles of 11 candidate unigenes were analyzed by quantitative real-time PCR (RT-qPCR). Our study established a global transcriptome database of P. heterophylla for gene identification and regulation. We also identified the candidate unigenes involved in triterpenoids saponins biosynthesis. Our results provide an invaluable resource for the secondary metabolites and physiological processes in different tissues of P. heterophylla.

KEGG

Secondary metabolism

10.1371/journal.pone.0164235

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Citations (15)

<div>A new species of Dichocarpum (Ranunculaceae) from Guizhou, China</div>

Phytotaxa (2015)

Wei-Ke Jiang Ling Ding Tao Zhou HE Shun-zhi Yunchao Li

Dichocarpum wuchuanense S. Z. He, a new species from Guizhou, China, is described and illustrated. This taxon belongs to sect. Dichocarpum based on its non-tumescent torus during fruiting. The new species resembles D. hypoglaucum from which it differs by its slender and longer rhizome, 3(‒5)-foliolate abaxially non-pruinose and smaller seeds. A histological examination, palynology and seed microscopic structures are provided along with detailed habitat and distribution data. An analysis of the nrDNA ITS sequences of D. wuchuanense supports its recognition as a taxonomic entity distinct from D. hypoglaucum. The taxonomic affinities of the new species are briefly discussed.

Ranunculaceae

Eudicots

Affinities

10.11646/phytotaxa.227.1.7

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[Technical evaluation and principle analysis of simulative habitat cultivation of Dendrobium nobile].

PubMed (2020)

Jinqiang Zhang Tao Zhou Chenghong Xiao Wei-Ke Jiang Lanping Guo

The technique of "simulative habitat cultivation" is to preserve the quality of traditional Chinese medicine by simulating the original habitat and site environment of wild Chinese medicine resources. Dendrobium nobile is the most representative variety of traditional Chinese medicine which reflects the coordinated development of medicinal material production and ecological environment. In this paper, the main technical points of the simulated cultivation model of D. nobile were summarized as follows: rapid propagation of seedling tissue technology to ensure the genetic stability of provenance; line card+fermented cow manure+live moss method to improve the survival rate; epiphytic stone cultivation to improve the quality of medicinal materials; and the integration of mycorrhizal fungi to improve the quality stability of medicinal materials. On the basis of summarizing the ecological benefits, economical and social benefits generated by the application of the technology, the paper systematically analyzes the principle of the technology for the cultivation of D. nobile to promote the excellent quality, the light, gas, heat and fertilizer resources of the undergrowth niche are in line with the wild site environment of D. nobile. The rich and complex soil microbial community in the forest laid the foundation for the species diversity needed for the growth of D. nobile.The stress effect on the growth of D. nobile resulted in the accumulation of secondary metabolites. The symbiotic relationship between the symbiotic fungi such as bryophytes and D. nobile promotes the synthesis of plant secondary metabolites. The high quality D. nobile was produced efficiently by improving and optimizing the cultivation techniques.

Biofertilizer

10.19540/j.cnki.cjcmm.20200302.110

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Association between dipsacus saponin VI level and diversity of endophytic fungi in roots of Dipsacus asperoides

World Journal of Microbiology and Biotechnology (2019)

Anhui Gong Tao Zhou Chenghong Xiao Wei-Ke Jiang Yongqiang Zhou

Dipsacus asperoides contains multiple pharmacologically active compounds. The principal are saponins. The plant can be cultivated, but it contains lower levels of bioactive compounds than the plant in the wild. It may be the reason to exploit the endophytic fungi that colonize the plant roots in order to produce bioactive compounds. However, the endophytic fungi of D. asperoides have not been analyzed in detail. In this study, we isolated and identified 46 endophytic fungal strains from the taproots, lateral roots and leaves, and we used morphological and molecular biological methods to assign them into 15 genera: Fusarium sp., Ceratobasidium sp., Chaetomium sp., Penicillium sp., Aspergillus sp., Talaromyces sp., Cladosporium sp., Bionectria sp., Mucor sp., Trichoderma sp., Myrothecium sp., Clonostachys sp., Ijuhya sp., Leptosphaeria sp. and Phoma sp. Taproots contained abundant endophytic fungi, the numbers of which correlated positively with level of dipsacus saponin VI. Primary fermentation of several endophytic fungal strains from taproots showed that Fusarium, Leptosphaeria, Ceratobasidium sp. and Phoma sp. can produce the triterpenoid saponin. These results may guide efforts to sustainably produce bioactive compounds from D. asperoides.

Phoma

Cladosporium

Chaetomium

Trichoderma

10.1007/s11274-019-2616-y

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Citations (32)

[Specific PCR Molecular Identification of Euconunmia Cortex].

PubMed (2015)

Ling Ding Dan Zhao Tao Zhou Wei-Ke Jiang Chenghong Xiao

To screen specific DNA sequence by rDNA-ITS sequence analysis at Eucommmia Cortex and its adulterants, then specific primers were designed to identify Eucommmia Cortex rapidly.rDNA-ITS sequences of Eucommmia Cortex and its adulterants were downloaded from NCBI. The specific DNA sequence in the rDNA-ITS sequence of Eucommmia Cortex was found by compared with all sequences. Specific primers for authentic the Eucommmia Cortex were designed. PCR amplification and detection methods were optimized.PCR amplification products were detected through agarose gel electrophoresis and fluorescence reaction. The 400 bp identification band for Eucommmia Cortex could be produced and green fluorescent could be observed under ultraviolet light after adding the dye SYBR Green I. However its adulterants were without identification hand and green fluorescent.Specific PCR molecular identification can be applied to identify Eucommmia Cortex, the time is shortened significantly by PCR amplification optimization, and fluorescence detection can be more visually display the identification results. In a word, this study provides new ideas and methods for rapid identification of Eucommmia Cortex.

SYBR Green I

Agarose gel electrophoresis

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Additional file 6 of Insight to shape of soil microbiome during the ternary cropping system of Gastradia elata

Figshare (2020)

Qing‐Song Yuan Jiao Xu Wei-Ke Jiang Xiaohong Ou Hui Wang

Additional file 6: Table S3. The relationship of changed microbiome at different compartment rhizoshphere at genus level.

10.6084/m9.figshare.12257810

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Citations (0)

Contamination of the traditional medicine Radix Dipsaci with aflatoxin B1 impairs hippocampal neurogenesis and cognitive function in a mouse model of osteoporosis

Ecotoxicology and Environmental Safety (2024)

Chengyan Yang Wei-Ke Jiang Dapeng Su Changgui Yang Qing‐Song Yuan

Radix (gastropod)

10.1016/j.ecoenv.2024.116831

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Citations (1)

[Analysis of medicinal resources diversity of 33 pilots (districts) in Guizhou province].

PubMed (2019)

Xiao-Qi Hou Wei-Ke Jiang Song PeiLang Shenghua Wei Qing-wen Sun

This study is based on the data analysis of medicinal plant resources and diversity collected from the fourth Chinese traditional medicine resource survey( pilot). Through the analysis of relevant data from 33 census pioneer plots in Guizhou province( area),a total of 265 families,1 432 genera and 5 296 species of medicinal resources were reported,including algae,fungi,lichens,mosses,a total of 43 genera and 35 families,57,48 families,120 genera and 453 species of ferns,gymnosperms 11 families,22 genera and 61 species,167 families,1 243 genera and 4 721 species of angiosperms,4 genera and 4 families four medicinal animals.Compared with the data related to the third survey of traditional Chinese medicine resources,the number of ferns,gymnosperms and angiosperms in the fourth survey has increased far more than that of the third survey. From the regional distribution of medicinal resources,the composition of the genus,the type of life,and the location of the medicine,the richness of the medicinal plant resources in Guizhou province is not only reflected in many types,but also in the variety of medicinal resources. These studies provide a scientific basis for vigorously developing the Chinese herbal medicine industry and the sustainably using medicinal plant resources in Guizhou province.

Floristics

10.19540/j.cnki.cjcmm.20181106.004

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Citations (1)

Pesudostellariae Radix industry status and development countermeasures

China Journal of Chinese Materia Medica (2016)

Wei-Ke Jiang Tao Zhou

In recent years, with increasing role in traditional Chinese medicine industry of many provinces in china, Pseudostellariae Radix is one of the rapid development traditional Chinese medicine. Based on references and on-site investigation of main producing area, we clear the process of change from the small of the Panax ginseng to the Pseudostellariae Radix in aspects of history, and the change of producing area from Shandong, Jiangsu provinces to Fujian, Anhui and Guizhou provinces. And we clear how the Pseudostellariae Radix become a commonly used Chinese medicinal herbs from a small variety based on the market supply and demand and price changes, and it has gradually become a hot topic of the research. This paper summarized the status quo and the existing problems in the Pseudostellariae Radix industry. On this basis, we put forward countermeasures and suggestions for the development of the Pseudostellariae Radix industry.

Radix (gastropod)

10.4268/cjcmm20161301

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Citations (2)

Research on quality regionalization of cultivated Pseudostellaria heterophylla based on climate factors

China Journal of Chinese Materia Medica (2016)

Chuan‐Zhi Kang Tao Zhou Wei-Ke Jiang Lanping Guo Xiaobo Zhang

Maxent model was applied in the study to filtering the climate factors layer by layer. Polysaccharides and pseudostellarin B the two internal quality evaluation index were combined to analyse the interlinkages between climate factors and chemical constituents in order to search for the critical climate factors of Pseudostellaria heterophylla. Then based on the key climate factors to explicit the quality spatial distribution of P. heterophylla. The results showed that polysaccharides and climatic factors had no significant correlation, suggesting that the indicator was not climate-driven metabolites. Pseudostellarin B could construct regression model with the precipitation. And quality regionalization results showed that pseudostellarin B content presented firstly increased and then decreased trend from southeast to northwest, which was the consistent change with precipitation. It clearly proposed that precipitation was the key climate factor, which affected the accumulation of cyclopeptide compound for Pseudostellariae Radix.

Radix (gastropod)

10.4268/cjcmm20161303

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Citations (4)