Fernanda Freire Campos Nunes

Universidade Federal de Minas Gerais

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Tânia Mara Pinto Dabés Guimarães

Universidade Federal de Minas Gerais

Jôsimar Dornelas Moreira

The University of Texas Health Science Center at Tyler

Luan Carlos Vieira Alves

Universidade Federal de Minas Gerais

Vicente de Paulo Coelho Peixoto de Toledo

Universidade Federal de Minas Gerais

Walter Batista Cicarini

Universidade Federal de Minas Gerais

María das Graças Carvalho Ferriani

Universidade Federal de Minas Gerais

Paulo Madureira de Pádua

Grupo Santa Casa de Belo Horizonte

Silvana Spíndola de Miranda

Universidade Federal de Minas Gerais

Cláudia Lopes Santoro Neiva

Grupo Santa Casa de Belo Horizonte

Renato Vargas Consoli

Grupo Santa Casa de Belo Horizonte

Cooperative Institutions

Universidade Federal de Minas Gerais

Universidade Federal do Piauí

Fundação Oswaldo Cruz

Universidade de São Paulo

Centro Universitário de Belo Horizonte

Universidade Estadual do Piau

Universidade Federal do Rio Grande do Sul

Centro Universitário UNINOVAFAPI

Universidade Federal de Sergipe

Universidade de Rio Verde

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Systemic lupus erythematosus

Blood Coagulation & Fibrinolysis (2018)

Walter Batista Cicarini Karine Silvestre Ferreira Cristina M.G. Loures Renato Vargas Consoli Cláudia Lopes Santoro Neiva

: To evaluate blood-borne endothelial microparticles (EMPs) in women with SLE and correlated these to disease activity as defined by the SLEDAI-2K score. The study takes cross-sectional design. A total of 90 age-matched women were recruited including: G1 (healthy volunteers, n = 30), G2 (women with SLE and low disease activity (SLEDAI-2K score ≤4; n = 30) and G3 (women with SLE and moderate/high disease activity (SLEDAI-2K score >4; n = 30). Blood was collected in 3.2% sodium citrate. Subsequently, the microparticles were purified by ultracentrifugation and labeled with anti-CD51/61 and anti-Annexin-V antibodies. Quantification and phenotyping were performed using flow cytometry. The number of EMPs was significantly higher in SLE patients compared with controls (P = 0.0178). When SLE patients were stratified according to disease activity, the number of EMPs was significantly increased in women with moderate-to-high disease activity compared with controls (P = 0.0074). We observed a correlation between the number of EMPs and age (r = -0.34; P = 0.0123) and between the number of EMPs and SLEDAI-2K score (r = 0.30; P = 0.04). Our results suggest that the SLE causes increased EMPs release, especially in patients with SLEDAI-2K score greater than 4. Although measurement of the EMPs could be useful in distinguishing patients with SLE from health controls, they have limited value in differentiating between SLE subtypes.

10.1097/mbc.0000000000000703

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Citations (5)

Polycystic Ovary Syndrome as a systemic disease with multiple molecular pathways: a narrative review

Endocrine Regulations (2018)

Laura Machado Lara Carvalho Fernando M. Reis Ana Lúcia Cândido Fernanda Freire Campos Nunes Cláudia N. Ferreira

Abstract Polycystic Ovary Syndrome (PCOS) is characterized by hyperandrogenism, amenorrhea, and polycystic ovaries. This endocrinopathy is associated with many metabolic disorders such as dyslipidemia and insulin resistance, with increased risk of type 2 diabetes mellitus, metabolic syndrome, and cardiovascular complications. Inflammation is likely to play an important role in the promoting these metabolic imbalances, while prothrombotic and pro-oxidative mechanisms further contribute to the cardiovascular risk of these patients. The etiology of PCOS is still not fully understood, but there is evidence of genetic and environmental components. This review aims to discuss some molecular pathways associated with PCOS that could contribute to the better understanding about this syndrome. Recent evidence suggests that intrauterine exposure of female mice to an excess of anti-Müllerian hormone may induce PCOS features in their post-natal life. High cytokine levels and cytokine gene polymorphisms also appear to be associated with the pathophysiology of PCOS. Furthermore, high levels of microparticles may contribute to the altered hemostasis and enhanced inflammation in PCOS. All these mechanisms may be relevant to clarify some aspects of PCOS pathogenesis and inspire new strategies to prevent the syndrome as well as treat its symptoms and mitigate the risk of long-term complications.

Hyperandrogenism

Dyslipidemia

Pathogenesis

Anovulation

10.2478/enr-2018-0026

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Citations (38)

A Leishmania murine model to evaluate the immunomodulatory properties of Pythium insidiosum proteins

Heliyon (2017)

Tatiana Maria Inêz-Ferreira Leonel Mendoza Raquel Vilela Hélida Monteiro de Andrade João Paulo Amaral Haddad

Pythium insidiosum immunomodulatory vaccine (PiV) has been tested in clinical and experimental pythiosis. Previous data showed that P. insidiosum immunogens have the ability to switch the Th2 immune response, normally in place during pythiosis, to a curative Th1 response. Pythiosis cannot be reproduced in experimental rodents with the exception of rabbits, and thus thorough evaluation of PiV´s immunomodulatory properties has been limited by the lack of a compatible inbred mouse model. In this study, we took advantage of the murine BALB/c Leishmania infection model, where infected mice produce a Th2 response, to evaluate the PiV Th2 to Th1 immunomodulatory potential. Twenty-one days following challenge with L. major, large cutaneous granulomas developed in control mice, consistent with the expected Th2 response. In contrast, Leishmania-induced cutaneous lesions in PiV-immunized mice were minimal or absent. Flow cytometry analysis of spleen cells from mice immunized with PiV and subsequently challenged with L. major displayed more CD4+ and CD8+ cells than the control group. Moreover, spleen cells from mice that were immunized with PiV then challenged with L. major secreted high levels of IFN-γ, with a moderate IL-2, IL-4, and IL-10 mixed cytokine profile upon in vitro re-stimulation with PiV. Anti-P. insidiosum IgG1 in immunized animals was present at low titers suggesting a minor immunological role for this Ig isotype in this model. Our preliminary data showed that BALB/c mice challenged with L. major represent an attractive model in which to study PiV´s immunomodulatory properties.

Isotype

10.1016/j.heliyon.2017.e00278

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Citations (4)

Evaluation of potential biomarkers for the diagnosis and monitoring of Systemic Lupus Erythematosus using the Cytometric Beads Array (CBA)

Clinica Chimica Acta (2019)

Luan Carlos Vieira Alves María das Graças Carvalho Ferriani Fernanda Freire Campos Nunes Edna Afonso Reis Gilda Aparecida Ferreira

10.1016/j.cca.2019.08.033

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Citations (8)

Pesquisas e Ações em Saúde Pública - Volume 2

Editora Pasteur eBooks (2021)

Maria Paula Bernardo dos Santos Franciane Ferreira Maziero Branco Jéssica Moreira Fernandes Larissa Brazolotto Ferreira Letícia Aparecida de Souza Silva

10.29327/531150

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Citations (0)

Comparative analysis of membrane microparticles (MP) expression in women with epithelial ovarian cancer (EOC) and endometriomas

Fertility and Sterility (2015)

J.A. Falcao Fernanda Freire Campos Nunes J. Marinuzzi Olindo Assis Martins‐Filho Andréa Teixeira‐Carvalho

10.1016/j.fertnstert.2015.07.487

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High Precision Method for the Determination of Ethyl Carbamate in Cachaça by HRGC-MS

Marcus Henrique Juliana Swensson de Mattos Fernanda Freire Campos Nunes Tânia Monteiro Raquel D. C. Cunha

HRGC-MS-SIM was used for determination of ethyl carbamate in commercial cachaca by a simple, fast and reliable technique for direct analysis, without the previous stage of extraction of the analyte of interest. The quantification technique used was the internal standardization with ethyl carbamate-d5. The following validation parameters were evaluated: Limit of Detection, Limit of Quantification, Linearity, Repeatability, Reproducibility, Accuracy and Uncertainty. The contributions that influenced the uncertainty in a great extent came from the repeatability and the accuracy. The characterization of the uncertainty sources and the ethyl carbamate determination, allowed Inmetro to certify its Certified Reference Material (CRM) for contaminants in cachaca.

Repeatability

Ethyl carbamate

Carbamate

Certified reference materials

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Cell phenotypes as activity biomarkers in patients with Systemic Lupus Erythematosus

Figshare (2023)

Cristina de Mello Gomide Loures Tânia Mara Pinto Dabés Guimarães Karine Silveste Ferreira Marcos Vinícius Ferreira Silva Luan Carlos Vieira Alves

Abstract The pathogenesis of systemic lupus erythematosus (SLE) is complex. Few studies in Brazilian population have addressed cell phenotypes associated with immunological responses and their associations with SLE activity. The aim of this study is to investigate cell phenotypes associated to SLE diagnosis, treatment and activity. Twenty-eight SLE female patients (17 inactive, 11 active) and 10 healthy women were included in this study. Markers of natural killer (Nk), T and B cells in peripheral blood were evaluated by flow cytometry. Nkt cells were decreased only in SLE active patients. Activated CD4+, regulatory T FoxP3+ and B cells were decreased in both active and inactive SLE patients, compared to control group. The data corroborate the disruption of immune regulatory response in SLE patients and suggest phenotipic changes as possible biomarkers of SLE activity.

Systemic lupus

10.6084/m9.figshare.22638723

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Citations (0)

Creatinine and cytokines plasma levels related to HLA compatibility in kidney transplant patients

Jornal Brasileiro de Patologia e Medicina Laboratorial (2015)

Lorraine Vieira Alves Marcelo José O. Maia Fernanda Freire Campos Nunes Henrique P.B. Magalhães Daniela Almeida Freitas Afonso

Introduction:The success of kidney transplantation depends on prevention of organ rejection by the recipient’s immune system, which recognizes alloantigens present in transplanted tissue. Human leukocyte antigen (HLA) typing is one of the tests used in pre-renal transplantation and represents one of the most important factors for a successful procedure.Objective:The present study evaluated creatinine and cytokines plasma levels in kidney transplant patients according to pre-transplant HLA typing.Methods:We assessed 40 renal transplanted patients selected in two transplant centers in Belo Horizonte (MG).Results:Patients were distributed into three groups according to HLA compatibility and, through statistical analysis, the group with more than three matches (H3) was found to have significantly lower post-transplant creatinine levels, compared to groups with three or fewer matches (H2 and H1, respectively). The median plasma levels of cytokines interleukin 6 (IL-6), tumor necrosis factor alpha (TNF-α), and interleukin 10 (IL-10) were evaluated according to the number of matches. Pro-inflammatory cytokines (IL-6 and TNF-α) were significantly higher in groups with lower HLA compatibility. On the other hand, the regulatory cytokine IL-10 had significantly higher plasma levels in the group with greater compatibility between donor and recipient.Conclusion:These findings allow us to infer that pre-transplant HLA typing of donors and recipients can influence post-transplant renal graft function and may contribute to the development and choice of new treatment strategies.

10.5935/1676-2444.20150049

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Citations (3)

Author response for "Circulating microparticles from subjects with active pulmonary tuberculosis infection modulate immune response"

Jôsimar Dornelas Moreira Fernanda Freire Campos Nunes Fernanda Cristina Gontijo Evangelista Henrique R. Silva Marcela M. Tonaco

Active tuberculosis

10.1002/eji.202048844/v3/response1

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