Wei-Kai Huang

National Cheng Kung University

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Xuyu Qian

Boston Children's Hospital

Yichen Cheng

Wuhan University

Zhexing Wen

Emory University

Guo‐li Ming

University of Pennsylvania

Hengli Tang

Florida State University

Emily M. Lee

National Institutes of Health

Kimberly M. Christian

University of Pennsylvania

Wei Zheng

Vanderbilt University Medical Center

Peng Jin

Emory University

Hongjun Song

Ocean University of China

Cooperative Institutions

Johns Hopkins University

Johns Hopkins Medicine

National Institutes of Health

National Center for Advancing Translational Sciences

National Cheng Kung University

Emory University

National Yang Ming Chiao Tung University

Chinese Academy of Sciences

University of Pennsylvania

National Taiwan University

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Molecular signatures associated with ZIKV exposure in human cortical neural progenitors

Nucleic Acids Research (2016)

Feiran Zhang Christy Hammack Sarah C. Ogden Yichen Cheng Emily M. Lee

Zika virus (ZIKV) infection causes microcephaly and has been linked to other brain abnormalities. How ZIKV impairs brain development and function is unclear. Here we systematically profiled transcriptomes of human neural progenitor cells exposed to Asian ZIKVC, African ZIKVM, and dengue virus (DENV). In contrast to the robust global transcriptome changes induced by DENV, ZIKV has a more selective and larger impact on expression of genes involved in DNA replication and repair. While overall expression profiles are similar, ZIKVC, but not ZIKVM, induces upregulation of viral response genes and TP53. P53 inhibitors can block the apoptosis induced by both ZIKVC and ZIKVM in hNPCs, with higher potency against ZIKVC-induced apoptosis. Our analyses reveal virus- and strain-specific molecular signatures associated with ZIKV infection. These datasets will help to investigate ZIKV-host interactions and identify neurovirulence determinants of ZIKV.

Zika Virus

Microcephaly

Flavivirus

10.1093/nar/gkw765

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Citations (162)

CCL5/RANTES is important for inducing osteogenesis of human mesenchymal stem cells and is regulated by dexamethasone

BioScience Trends (2014)

Yu-Chuan Liu Yu-Ting Kao Wei-Kai Huang Kun-Yi Lin Shinn‐Chih Wu

In this study, we examine the effect of chemokine (C-C motif) ligand 5 (CCL5)/Regulated on Activation Normal T cell Expressed and Secreted (RANTES), a pro-inflammatory cytokine on osteogenic differentiation of human mesenchymal stem cells (hMSCs). We found CCL5 expression was increased during osteogenic differentiation of hMSCs and CCL5 expression is dependent on the presence of dexamethasone. Knocking down endogenous CCL5 expression blocked osteogenesis, as revealed by decreasing alkaline phosphatase (ALP) activity and a reduction in the expression levels of ALP, bone sialoprotein (BSP), and osteopontin (OPN). Of note, the overexpression of CCL5 was sufficient to increase ALP expression and activity. Moreover, the down-regulation of chemokine (C-C motif) receptor 1 (CCR1), one of the CCL5 receptors, significantly decreased the osteogenesis of hMSCs. Interestingly, the down-regulation of CCR1, but not CCL5, was sufficient to affect the cell numbers during the process of osteogenesis. Our findings reveal that both CCL5 and CCR1 are required for osteogenesis of human MSCs, CCL5 is sufficient for the osteogenesis, and provide a novel link between dexamethasone and CCL5 in human osteogenesis.

CCL5

Osteopontin

10.5582/bst.2014.01047

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Citations (42)

Identification of small-molecule inhibitors of Zika virus infection and induced neural cell death via a drug repurposing screen

Nature Medicine (2016)

Miao Xu Emily M. Lee Zhexing Wen Yichen Cheng Wei-Kai Huang

Zika Virus

Drug repositioning

Repurposing

Niclosamide

Antiviral drug

Flavivirus

Kinome

10.1038/nm.4184

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Citations (632)

Zika-Virus-Encoded NS2A Disrupts Mammalian Cortical Neurogenesis by Degrading Adherens Junction Proteins

Cell stem cell (2017)

Ki‐Jun Yoon Guang Song Xuyu Qian Jianbo Pan Dan Xu

Adherens junction

Forebrain

10.1016/j.stem.2017.07.014

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Citations (194)

Relaxation Dynamics and Structural Characterization of Organic Nanobelts with Aggregation-Induced Emission

The Journal of Physical Chemistry C (2012)

Chih-Wei Chang Chetan Jagdish Bhongale Chi‐Shen Lee Wei-Kai Huang Chain‐Shu Hsu

We prepared the fluorescent nanobelts of cyano-substituted 1,4-distyrylbenzene derivative (CNDSB) with the reprecipitation method. CNDSB is nonemissive in solution but with strong aggregation-induced emission (AIE) in belt-like structure. The molecular structure predicted by quantum chemical calculations indicates a twisted conformation of CNDSB monomer, while the solid-state X-ray structure of the CNDSB crystal features herringbone-type arrangement and three benzene rings arrange in nearly planar conformation. Femto- and picosecond time-resolved fluorescence spectroscopy are implemented to study the photophysics and the origin of the AIE effect in CNDSB nanobelts. The fluorescence quenching of CNDSB in THF solution arises from the efficient nonradiative isomerization channel. In this study, the fluorescence decay of CNDSB embedded in poly(methyl methacrylate) matrix is slowed down due to the restriction of intramolecular motions in the solid matrix. In nanobelt structure, the fluorescence lifetime further increases due to the intermolecular interaction between H-type aggregates. Both the steady-state and time-resolved studies suggests that there are at least two different packing structures might exist in nanobelts, and the relative composition of these two different structures gradually changes in different sizes of nanobelts.

Picosecond

10.1021/jp304117n

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Citations (40)

Plasminogen/thrombomodulin signaling enhances VEGF expression to promote cutaneous wound healing

Journal of Molecular Medicine (2018)

Tsung‐Lin Cheng Po-Ku Chen Wei-Kai Huang Cheng‐Hsiang Kuo Chia-Fong Cho

Thrombomodulin

10.1007/s00109-018-1702-1

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Citations (23)

Chicken Essence Improves Exercise Performance and Ameliorates Physical Fatigue

Nutrients (2014)

Wen-Ching Huang Ching-I Lin Chien-Chao Chiu Yi‐Ting Lin Wei-Kai Huang

Chicken essence (CE) is a liquid nutritional supplement made from cooking whole chickens. In traditional Chinese medicine, CE is used to support health, promote healing, increase metabolism, and relieve fatigue. However, few studies have examined the effect of CE on exercise performance and physical fatigue. We aimed to evaluate the potential beneficial effects of CE on fatigue and ergogenic functions following physical challenge in mice. Male ICR mice were divided into four groups to receive vehicle or CE by oral gavage at 0, 845, 1690, or 4225 mg/kg/day for 4 weeks. Exercise performance and anti-fatigue function were evaluated by forelimb grip strength, exhaustive swimming time, and levels of physical fatigue-related biomarkers serum lactate, ammonia, glucose, and creatine kinase (CK) after physical challenge. CE supplementation dose-dependently elevated endurance and grip strength. CE supplementation significantly decreased lactate, ammonia, and CK levels after physical challenge. Tissue glycogen content, an important energy source for exercise, was significantly increased with CE supplementation. In addition, CE supplementation had few subchronic toxic effects. The supplementation with CE can have a wide spectrum of bioactivities on health promotion, performance improvement and anti-fatigue.

Creatine

Creatine kinase

10.3390/nu6072681

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Citations (46)