Susmita Saha

Tripura University

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Samir Kumar Sil

Tripura University

Jhinuk Basu Mullick

University of Florida

Debaprasad Chakraborty

Tripura Medical College & Dr. B.R. Ambedkar Memorial Teaching Hospital

Parimal Karmakar

Jadavpur University

Partha Sarathi Saha

Tripura University

Manikarna Dinda

University of Virginia

Kanak Chakraborty

Tripura University

Shipa Begam

Tripura University

Angkita Debnath

Tripura University

Parichita Ray Choudhury

Tripura University

Cooperative Institutions

Tripura University

Jadavpur University

National Institute of Technology Agartala

Agartala Government Medical College

National Institute of Biomedical Genomics

R. G. Kar Medical College and Hospital

Indian Institute of Technology Guwahati

Indian Council of Medical Research

National Institute for Research in Reproductive Health

Ambedkar University Delhi

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Wound healing promoting activity of Earthworm, Eutyphoeus gammiei (Beddard): in vitro studies on human skin keratinocyte cell line (HaCat).

Journal of Drug Delivery and Therapeutics (2018)

Susmita Saha Deepjyoti Bhattacharjee Anwesha Saha Gahin De Partha Sarathi Saha

Earthworm, Eutyphoeus gammiei, homogenate (EGH) was screened for wound healing activity on human keratinocyte cell line, HaCat, by cell proliferation and migration assays. The maximum proliferation and migration of keratinocyte cells were observed at the dose of 25μg/ml. As cell proliferation and migration are key factors for wound healing, the study clearly suggests the potential role of earthworm species Eutyphoeus gammiei on wound healing. Keywords: Eutyphoeus gammiei, Keratinocyte, MTT assay, scratch assay.

HaCaT

MTT assay

10.22270/jddt.v8i6.2036

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IN VITRO TOXICITY STUDIES ON THE EXTRACT OF MEDICINAL PLANT EVOLVULUS NUMMULARIUS AS A POTENT MICROBICIDAL CANDIDATE

Journal of Drug Delivery and Therapeutics (2018)

Jhinuk Basu Mullick KVR Reddy Susmita Saha Tahir Bashir Samrat Hore

The herb Evolvulus nummularius (L). L generally grown as an ornamental plant. This herb has found many applications in traditional folk medicine. There was however insufficient scientific data to back its safety to be used on humans. Methanolic extract of E. nummularius was used to check for its safety as a vaginal microbicide through various safety tests such as cell viability using MTT assay on three female genital tract epithelial cell lines, vaginal (VK2/E6E7), endocervical (End1/E6E7) and endometrial (HEC-1-A). Quantification of hemolytic activity was done on human red blood cells (RBCs). Determination of cellular integrity was checked by transepithelial electrical resistance (TER) assay and permeability by fluorescent microsphere assay. At 24 hours, application of the extract for cell viability assay showed extensive cell death with cell disruption. IC50 of VK2/E6E7 and HEC-1-A cells were found to be 2 mg/ml, IC50of End1/E6E7 was 1 mg/ml. For hemolytic assay, with treatment of the extract for one hour did not show hemolysis till the concentration of 2.5mg/ml. In TER and microsphere permeability assays, polarized HEC-1-A monolayer 24 hours post treatment had significant drop in TER and enhanced fluorescence from passage of microspheres implying disruption of the epithelial monolayer. The study revealed the crude methanolic extract appeared to be toxic towards human RBCs and female genital tract epithelial cells. Due to its toxic nature, its direct applications to the human vaginal tissue in vivo should be done with caution. Keywords: Medicinal plants; Microbicide; Evolvulus nummularius (L). L; MTT assay; Transepithelial electrical resistance; Fluorescent microsphere assay. Â Â

Viability assay

Genital tract

MTT assay

10.22270/jddt.v8i4.1778

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REACTIVE OXYGEN SPECIES AS POSSIBLE MEDIATOR OF ANTIBACTERIAL ACTIVITY OF PARKIA JAVANICA, AGAINST BACTERIAL SPECIES PREDOMINANTLY FOUND IN CHRONIC WOUND.

Journal of Drug Delivery and Therapeutics (2018)

Samir Kumar Sil Susmita Saha Parimal Karmakar

The crude methanol extract of Parkia javanica was screened for antibacterial activity. against bacterial species predominantly found in chronic wound, by serial dilution technique. Growth kinetics study was performed and percentage of ROS production was measured by NBT reduction assay. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were obtained with a range of IC100 5-40 mg/ml in case of standard bacterial strains. The lag phase of all extract treated bacteria is extended compared to untreated cells. The normalized % of ROS is increased in presence of crude extract. This study suggests that the crude methanol extract of Parkia javanica possesses promising antimicrobial substances which are having activity against Standard ATCC bacterial species and ROS induced DNA damage could be the possible mediator of its antimicrobial activity. Keywords: Parkia javanica, antibacterial activity, standard ATCC bacterial strains, growth curve, ROS, DNA damage

10.22270/jddt.v8i1.1657

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FIRST REPORT ON FIBRINOLYTIC AND THROMBOLYTIC ACTIVITY OF EUTYPHOEUS GAMMIEI AN EARTHWORM SPECIES COLLECTED FROM TRIPURA, NORTHEAST INDIA

Asian Journal of Pharmaceutical and Clinical Research (2018)

Madhusudan Debnath Susmita Saha Samir Kumar Sil

Objective: The present investigation for the first time evaluated the in vitro fibrinolytic and thrombolytic activities of crude extracts from Eutyphoeus gammiei, native, large size earthworm of Tripura, Northeast, India. The present study was designed to evaluate the therapeutic use of the organism E. gammiei as a source of fibrinolytic and thrombolytic agent(s).Methods: The fibrinolytic activity was studied using by fibrin plate and zymography assays. Thrombolytic assay was carried out according to Prasad et al. (2006) using whole blood.Results: The results obtained clearly indicated E. gammiei as a potential source of fibrinolytic and thrombolytic agents. Both in fibrin plate assay and thrombolytic assay with whole blood, E. gammiei crude homogenate showed similar and close results in respect to that of streptokinase. Fibrin zymography also showed antifibrinolytic activity with producing clear bands. Dose and time dependency also is evident from the results.Conclusion: The results of the present study conclude that the studied earthworm species E. gammiei possessed profound fibrinolytic and thrombolytic activity on human blood and E. gammiei might prove to be useful alternative source for the development of new drugs for treatments involving blood coagulation and fibrinolysis.

Fibrinolytic agent

Zymography

10.22159/ajpcr.2018.v11i11.27739

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Cervical cytology abnormalities and associated genotype patterns of High-Risk HPV Infection in Women of Tripura, North-East India: A Hospital-Based Study

American Journal of Epidemiology (2024)

Amrapali Bhattacharya Kanak Chakraborty Partha Sarathi Saha Susmita Saha Parichita Roychaudhuri

Abstract The current study explored HPV prevalence and age variation in cervical samples of different cytological categories and HPV types from women seeking gynecological care in Tripura, northeast India. Pap smears, cervical tissues, and HPV/DNA specimens were collected from gynecological outpatient departments (OPD) or in-house patients and were screened for HPV16, HPV18, and other HPV types by PCR. Finally, logistic regression was performed to find the association between epidemiologic factors and HPV infection in women of different cytological grades. About 90% of HPV-screened women were found to be HPV positive. HPV16 was most common in HPV-positive women (53.27%), followed by HPV16/18 co-infection (26.17%). HPV16 or HPV18 was most frequent (86.45%) compared to others (13.55%). Among the confirmed cytological tests 68.83% showed normal cytology and 37.17% showed atypical abrasions. Among the abnormal cytology participants, 94.37% were HPV-positive, and 42.25% had cervical cancer. The prevalence of HPV increased with cytological abnormalities (p < 0.01). Abnormal cytological lesions increased with age (p trend = 0.017). Among all epidemiologic factors studied, parity was strongly associated with overall HPV infection, regardless of cytological status. Observed very high frequency of HPV infection in the current study, warrants further investigations.

10.1093/aje/kwae364

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A silver iodide nanoparticle containing plant extract-based gelatinous composite for antibacterial coating applications

Applied Nanoscience (2022)

Sumati Malik Susmita Saha Samir Kumar Sil Chitraniva Datta Subhojit Das

Nanochemistry

10.1007/s13204-022-02694-1

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Antibacterial Activity of Evolvulus nummularius Against Standard ATCC Gram Positive and Gram Negative Strains: Studies on MIC, MBC, Growth Curve Analysis and ROS Generation

International Journal of Pure & Applied Bioscience (2016)

Susmita Saha

Gram

Gram-Negative Bacteria

10.18782/2320-7051.2357

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Studies on occurrence and distribution of Acinetobacter spp. and other gram-negative bacteria from meat

Journal of Food Science and Technology-mysore (2001)

Susmita Saha Balu A. Chopade

A total of 98 bacterial strains were recovered employing 5 different media from 12 retail meat samples, belonging to 3 genera consisting of 28 Acinetobacter (28.57%), 23 Moraxella (23.4%) and 47 Pseudomonas (47.96%) in different conditions. Acinetobacter was found to be the dominant organism (38.25%), followed by Pseudomonas (32.72%) in meat stored at refrigerator temperature. Though Pseudomonas was dominant in fresh (43.6%) and spoiled meat (42.9%) and meat stored at freezer temperature (-20°C for 20 days) (83.9%), while Acinetobacter was found to be the next dominant bacterium 31.6%, 18.5% and 10.39%, respectively from the above three categories of meat. Holton's medium without cefsulodin was found to be the suitable medium for the recovery of Acinetobacter from meat. 98 souches bacteriennes ont ete isolees en utilisant 5 milieux de culture dans la viande commercialisee. Elles appartiennent aux 3 genres suivants: Acinetobacter (28,57%), Moraxella (23,4%), Pseudomonas (47,96%), les proportions dependant des conditions du milieu. Acinetobacter est la bacterie principale dans la viande refrigeree, alors que Pseudomonas l'est dans la viande fraiche, dans la viande alteree et congelee, acinetobacter etant la seconde population dominante dans ces conditions. Acinetobacter a ete isolee sur le milieu de Holton.

Moraxella (Branhamella) catarrhalis

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Increased ROS Generation: Implication in Antibacterial Activity of Evolvulus nummularius against Multidrug Resistant Gram Negative Bacterial Strains

International Journal of Current Microbiology and Applied Sciences (2017)

Susmita Saha Anindya Sundar Das Angkita Debnath Shipa Begam Subrata Sen

Evolvulus nummularius, is ethnomedicinally used as a medicine for hysteria, to cure burns, cuts, wounds and scropion stings. This plant has antihelminthic activity, wound healing activity, poor sedative and anticonvulsant properties. In this study, the antibacterial activity of crude methanol extract of E. nummularius was studied against three gram negative multidrug resistant strains E. aerogenes, P. aeruginosa and K. pneumoniae. This plant extract showed both bacteriostatic and bactericidal activity against all these bacterial strains. The extract was most active against E. aerogenes. In growth analysis study, it was obtained that, after treatment with IC50 dose for each bacterial strains, the lag phase become extended compared to untreated bacterial cells. To understand the mechanism of action of Evolvulus nummularius the reactive oxygen species (ROS) was estimated and result showed that, reactive oxygen species were increased (~ 35-60%) in presence of IC50 dose of Evolvulus nummularius.

Gram

10.20546/ijcmas.2017.601.013

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Anti-Vibrio Activity of Parkia javanica: Studies on MIC, MBC, Growth Curve Analysis and ROS Generation on Four Vibrio cholarae Strains

International Journal of Current Microbiology and Applied Sciences (2016)

Susmita Saha Jhinuk Basu Mullick Parichita Ray Choudhury Partha Saha Debaprasad Chakraborty

Parkia javanica, a medicinal plant, of North-East India, was screened for anti cholera activity against 4 Vibrio cholarae strains. Fresh stem barks of Parkia javanica was collected and crude methanol extract of Parkia javanica (MEPJ) was prepared by Hahnshin rotary evaporator. The minimum inhibitory concentration of MEPJ was evaluated on Vibrio cholarae strains by serial dilution technique. 10mg/ml of MEPJ is the IC100 dose for Vibrio cholarae strains. MEPJ also showed bactericidal activity against all four Vibrio cholarae at the same concentration. The lag phase become extended after treatment with IC50 dose of MEPJ. Intracellular reactive oxygen species (ROS) was increased (40% - 60%) in presence of MEPJ indicating ROS mediated mechanism of growth inhibition.

10.20546/ijcmas.2016.508.058

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