Yasuhiro Takahata

Kyushu Okinawa Agricultural Research Center

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Masaru Tanaka

Kyushu Okinawa Agricultural Research Center

Takahiro Noda

Hokkaido Agricultural Research Center

Masaru Yoshinaga

Kyushu Okinawa Agricultural Research Center

Hiroki Nakayama

Tokyo National Hospital

N. Kaizuma

Iwate University

T. Sato

International University Of Health And Welfare Atami Hospital

Makoto Nakatani

Miyazaki Prefectural Wood Utilization Research Center

‍K

‍Kenji Katayama

National Agriculture and Food Research Organization

Tadahiro Nagata

Seitoku University

Tetsufumi Sakai

Kyushu Okinawa Agricultural Research Center

Cooperative Institutions

National Agriculture and Food Research Organization

Kyushu Okinawa Agricultural Research Center

Iwate University

Kazusa DNA Research Institute

Kyoto University

Institute of Crop Science

The University of Tokyo

Ministry of Agriculture, Forestry and Fisheries

Tohoku Agricultural Research Center

Ministry of Agriculture Forestry and Fisheries

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Varietal Differences in Crude Protein, and Free and Hydrolyzed Amino Acid Compositions of Sweetpotato Roots.

Nippon Shokuhin Kagaku Kogaku Kaishi (1995)

Yasuhiro Takahata Takahiro Noda Tadahiro Nagata

Crude protein content, and free and hydrolyzed amino acid compositions of whole root flour of sweetpotato cultivars that included lines lacking β-amylase were investigated in 1990 and 1991. A statistic varietal difference in crude protein content was detected; however, the order of the rank was largely different between the two years. The amino acid composition of whole root flour was similar among the cultivars and their profile was almost the same as in previous reports, although the aspartic acid content varied most greatly among the cultivars. The most predominant free amino acid was always asparagine in every cultivar, whereas the total free amino acid content largely varied among the cultivars. There was a large varietal difference in free asparagine content, which was quite altered between 1990 and 1991. However, the correlation coefficients between free asparagine content and hydrolyzed aspartic acid content in both years were above 0.9. Thus, most of the variabilities in aspartic acid contents among cultivars were the results of the variations in free asparagine content.

Aspartic acid

10.3136/nskkk.42.362

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Citations (7)

Highly Polymerized Procyanidins in Brown Soybean Seed Coat with a High Radical-Scavenging Activity

Journal of Agricultural and Food Chemistry (2001)

Yasuhiro Takahata Mayumi Ohnishi‐Kameyama Shu Furuta Masakazu Takahashi Ikuo Suda

1,1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging activity of the 70% aqueous acetone extract from the seed coat of the brown soybean variety, Akita-Zairai, was investigated. The activity of the seed coat of Akita-Zairai was much higher than that of three other reddish-brown varieties, but lower than that of two black varieties, and was closely dependent on the content of phenolic compounds. In the LH20 column chromatography of Akita-Zairai, high DPPH radical-scavenging activities were detected in the fractions eluted with MeOH and 70% aqueous acetone. Proanthocyanidins were also detected in fractions showing high radical-scavenging activities. Matrix-assisted laser desorption/ionization−time-of-flight mass spectrometry analysis showed that the degree of polymerization (DP) of the procyanidins contained in the brown or black soybean seed coat was as high as DP30. Keywords: Soybean; Glycine max; procyanidin polymer; radical-scavenging activity; brown seed coat; MALDI−TOFMS

Scavenging

Coat

Catechol

10.1021/jf010307x

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Citations (175)

Utilization of Microspore-Derived Embryos

Biotechnology in agriculture and forestry (2005)

Yasuhiro Takahata Hiroyuki Fukuoka Kenji Wakui

10.1007/3-540-26889-8_8

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Citations (16)

Genetic analysis of shoot regeneration from cotyledonary explants in Brassica napus

Theoretical and Applied Genetics (2000)

Y. Ono Yasuhiro Takahata

Organogenesis

Plant biochemistry

Maternal effect

10.1007/s001220051367

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Citations (16)

Haploid-resolved and chromosome-scale genome assembly in hexa-autoploid sweetpotato (Ipomoea batatas(L.) Lam)

bioRxiv (Cold Spring Harbor Laboratory) (2022)

Ung-Han Yoon Qinghe Cao Kenta Shirasawa Hong Zhai Tae‐Ho Lee

Abstract Sweetpotato ( Ipomoea batatas (L.) Lam) is the world’s seventh most important food crop by production quantity. Cultivated sweetpotato is a hexaploid (2n = 6x = 90), and its genome (B1B1B2B2B2B2) is quite complex due to polyploidy, self-incompatibility, and high heterozygosity. Here we established a haploid-resolved and chromosome-scale de novo assembly of autohexaploid sweetpotato genome sequences. Before constructing the genome, we created chromosome-scale genome sequences in I. trifida using a highly homozygous accession, Mx23Hm, with PacBio RSII and Hi-C reads. Haploid-resolved genome assembly was performed for a sweetpotato cultivar, Xushu18 by hybrid assembly with Illumina paired-end (PE) and mate-pair (MP) reads, 10X genomics reads, and PacBio RSII reads. Then, 90 chromosome-scale pseudomolecules were generated by aligning the scaffolds onto a sweetpotato linkage map. De novo assemblies were also performed for chloroplast and mitochondrial genomes in I. trifida and sweetpotato. In total, 34,386 and 175,633 genes were identified on the assembled nucleic genomes of I. trifida and sweetpotato, respectively. Functional gene annotation and RNA-Seq analysis revealed locations of starch, anthocyanin, and carotenoid pathway genes on the sweetpotato genome. This is the first report of chromosome-scale de novo assembly of the sweetpotato genome. The results are expected to contribute to genomic and genetic analyses of sweetpotato.

Sequence assembly

Ipomoea

10.1101/2022.12.25.521700

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Citations (10)

Relationships between chain length distribution of amylopectin and gelatinization properties within the same botanical origin for sweet potato and buckwheat

Carbohydrate Polymers (1998)

Takahiro Noda Yasuhiro Takahata T. Sato Ikuo Suda Toshikazu Morishita

Starch gelatinization

Molar mass

10.1016/s0144-8617(98)00047-2

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Citations (273)

Scanning electron microscopy of desiccation-tolerant and -sensitive microspore-derived embryos of Brassica napus L.

Plant Cell Reports (1999)

Kenji Wakui Yasuhiro Takahata N. Kaizuma

Desiccation Tolerance

10.1007/s002990050628

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Citations (18)

GENIC MALE STERILITY IN BRASSICA CAMPESTRIS L.

Acta Horticulturae (1996)

Yasuhiro Takahata Minoru Nagasaka H Kondo N. Kaizuma

10.17660/actahortic.1996.407.17

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Citations (6)

Structural Characterization of the Dihydroflavonol 4-reductase B (DFR-B) Gene in the Sweet Potato

DNA sequence (2004)

Masaru Tanaka Makoto Nakatani Yoshinori Nakazawa Yasuhiro Takahata

AbstractA genomic fragment containing the dihydroflavonol 4-reductase B (DFR-B) gene was cloned from the sweet potato (Ipomoea batatas) and its nucleotide sequence was analyzed. The exons and flanking regions were highly homologous to those of previously reported DFR-B genes of the Japanese morning glory, whereas the introns and the intergenic region were less conserved. In addition to the sequences of three miniature inverted-repeat transposable elements (MITEs) and one direct repeat previously reported in the DFR-B gene of Japanese morning glory, two mobile element-like sequences were newly identified in the sweet potato DFR-B gene. At least four allelic sequences were found to exist by amplification of the DFR-B gene from various sweet potato cultivars. One of these allelic sequences had a 2-kb deletion in the intergenic region and was observed in the cultivars with high anthocyanin content in their storage roots.KeywordsSweet potatoAnthocyaninDihydroflavonol 4-reductaseMobile elementMiniature inverted-repeat transposable elementTransposonAB112668AB112669AB112545

Ipomoea

Intergenic region

10.1080/10425170400004120

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Citations (8)

Nondestructive determination of fatty acid composition of husked sunflower (Helianthus annua L.) seeds by near‐infrared spectroscopy

Journal of the American Oil Chemists Society (1995)

T. Sato Yasuhiro Takahata Takahiro Noda Takashi Yanagisawa Toshikazu Morishita

Abstract Determination of the fatty acid composition of sunflower ( Helianthus annua L.) seeds by near‐infrared (NIR) spectroscopy was examined. Sunflower seeds were husked (removed from their hulls by a husking machine or manually with a knife). NIR spectra of these seeds were scanned from 1100 to 2500 nm at 2‐nm intervals in a whole‐grain cell with a wideangle moving drawer for machine‐husked seeds or in a single‐grain cup for a manually husked single‐grain seed. The extracted oils from machine‐husked seeds also were scanned by sandwiching them between a pair of slide glasses to create a thin layer and by placing them on a syrup cup. For extracted oil, the absorption band around 1720 nm filled out to the shorter wavelength region in the NIR second‐derivative spectra as the percentage of the linoleic acid moiety increased, because linoleic acid absorbs in this region. On the other hand, for husked seeds and for a single‐grain seed, as the percentage of linoleic acid increased, the trough at 1724 nm where oleic and saturated acids absorb decreased in the second‐derivative NIR spectra. Determination of the fatty acid composition of sunflower seeds could be carried out successfully according to the NIR spectral pattern for both extracted oil (r=−0.989) and kernel seed (r=−0.993). This is important, especially for a manually husked single‐grain seed (r=−0.971), because it can still be germinated after such nondestructive analysis.

Helianthus annuus

Sunflower seed

Sunflower oil

10.1007/bf02540985

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Citations (52)