Anna Carolina Borges Pereira Costa

Concordia University

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Antônio Olavo Cardoso Jorge

Universidade Estadual Paulista (Unesp)

Juliana Campos Junqueira

Universidade Estadual Paulista (Unesp)

Cristiane Aparecida Pereira

Universidade Estadual Paulista (Unesp)

Malcolm Whiteway

Concordia University

Aguida Maria

Instituto Adolfo Lutz

Guilherme Rodrigues Teodoro

Universidade Estadual Paulista (Unesp)

Vanessa Dumeaux

Western University

Raha Parvizi Omran

Concordia University

Vanessa Maria Campos Rasteiro

Universidade Estadual Paulista (Unesp)

Milton Beltrame

Universidade do Vale do Paraíba

Cooperative Institutions

Universidade Estadual Paulista (Unesp)

Universidade de São Paulo

Concordia University

Universidade do Vale do Paraíba

University of Aberdeen

Medical Research Council

Universidade de Taubaté

Institute of Science and Technology

ARC Centre of Excellence in Synthetic Biology

Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. - Hans-Knöll-Institut (HKI)

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Comparison of the effect of rose bengal- and eosin Y-mediated photodynamic inactivation on planktonic cells and biofilms of Candida albicans

Lasers in Medical Science (2013)

Fernanda Freire Anna Carolina Borges Pereira Costa Cristiane Aparecida Pereira Milton Beltrame Juliana Campos Junqueira

Rose bengal

Eosin

10.1007/s10103-013-1435-x

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Citations (52)

Photodynamic inactivation of virulence factors of Candida strains isolated from patients with denture stomatitis

Journal of Photochemistry and Photobiology B Biology (2015)

Cristiane Aparecida Pereira Nádia Domingues Michelle Peneluppi Silva Anna Carolina Borges Pereira Costa Juliana Campos Junqueira

Virulence factor

10.1016/j.jphotobiol.2015.08.029

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Citations (14)

Susceptibility of Candida albicans and Candida dubliniensis to erythrosine- and LED-mediated photodynamic therapy

Archives of Oral Biology (2011)

Anna Carolina Borges Pereira Costa Vanessa Maria de Campos Rasteiro Cristiane Aparecida Pereira Emily Setsuko Halter da Silva Hashimoto Milton Beltrame

The effect of erythrosine- and LED-mediated photodynamic therapy (PDT) on planktonic cultures and biofilms of Candida albicans and Candida dubliniensis was evaluated. Planktonic cultures of standardized suspensions (10(6)cells/mL) of C. albicans and C. dubliniensis were treated with erythrosine concentrations of 0.39-200 μM and LEDs in a 96-well microtiter plate. Biofilms formed by C. albicans and C. dubliniensis in the bottom of a 96-well microtiter plate were treated with 400 μM erythrosine and LEDs. After PDT, the biofilms were analysed by scanning electron microscopy (SEM). The antimicrobial effect of PDT against planktonic cultures and biofilms was verified by counting colony-forming units (CFU/mL), and the data were submitted to analysis of variance and the Tukey test (P<0.05). C. albicans and C. dubliniensis were not detectable after PDT of planktonic cultures with erythrosine concentrations of 3.12 μM or higher. The CFU/mL values obtained from biofilms were reduced 0.74 log(10) for C. albicans and 0.21 log(10) for C. dubliniensis. SEM revealed a decrease in the quantity of yeasts and hyphae in the biofilm after PDT. In conclusion, C. albicans and C. dubliniensis were susceptible to erythrosine- and LED-mediated PDT, but the biofilms of both Candida species were more resistant than their planktonic counterparts.

Erythrosine

Candida dubliniensis

10.1016/j.archoralbio.2011.05.013

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Citations (76)

Candida albicans Mrv8, is involved in epithelial damage and biofilm formation

FEMS Yeast Research (2020)

Anna Carolina Borges Pereira Costa Graziella Nuernberg Back‐Brito François L. Mayer Bernhard Hube Duncan Wilson

Candida albicans is the most common human fungal pathogen that can cause superficial and deep-seated infections in susceptible individuals. Despite its medical importance, the vast majority of C. albicans genes remain of unknown function. Here, we report a role for the lineage-specific gene, MRV8, in host pathogen interactions, mycelial microcolony maturation and biofilm formation. In silico analysis indicated that MRV8 encodes a four-pass transmembrane protein unique to the closely related pathogens C. albicans and Candida dubliniensis. Deletion of MRV8 did not affect C. albicans adherence to, or initial invasion into human oral epithelia, but inhibited mycelial development and strongly reduced epithelial damage. mrv8Δ/Δ cells exhibited a media-dependent defect in biofilm formation and mutant biofilm metabolic activity was enhanced by cyclosporin A. mrv8Δ/Δ biofilms were more tolerant to treatment with caspofungin, but not to fluconazole or amphotericin B. Co-stimulation with calcium chloride and calcofluor white rescued biofilm growth in the presence of caspofungin, and this rescue-effect was Mrv8-dependent. Together, our data demonstrate an important role for a lineage-specific gene (MRV8) in C. albicans biofilm formation, drug tolerance and host-pathogen interactions.

Human pathogen

10.1093/femsyr/foaa033

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Citations (4)

Susceptibility of Candida albicans and Candida dubliniensis planktonic cultures and biofilms to erythrosine- and LED-mediated photodynamic therapy

Mycoses (2011)

C. A. Pereira-Correia Anna Carolina Borges Pereira Costa Vanessa Maria Campos Rasteiro Milton Beltrame Juliana Campos Junqueira

Candida dubliniensis

Erythrosine

10.1111/myc.2011.54.issue-s2/issuetoc

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Citations (2)

Signal-mediated localization of Candida albicans pheromone response pathway components

G3 Genes Genomes Genetics (2020)

Anna Carolina Borges Pereira Costa Raha Parvizi Omran Chris Law Vanessa Dumeaux Malcolm Whiteway

A MAPK cascade consists of three kinases, (MEKK, MEK and MAPK), that are sequentially activated in response to a stimulus and serve to transmit signals. In C. albicans and in yeast, an MAPK cascade is linked to the pheromone pathway through a scaffold protein (Cst5 and Ste5, respectively). Cst5 is much shorter and lacks key domains compared to Ste5, so in C. albicans, other elements, in particular the MEKK Ste11, play key roles in controlling the associations and localizations of network components.Candida albicans opaque cells release pheromones to stimulate cells of opposite mating type to activate their pheromone response pathway. Although this fungal pathogen shares orthologous proteins involved in the process with Saccharomyces cerevisiae, the pathway in each organism has unique characteristics. We have used GFP-tagged fusion proteins to investigate the localization of the scaffold protein Cst5, as well as the MAP kinases Cek1 and Cek2, during pheromone response in C. albicans. In wild-type cells, pheromone treatment directed Cst5-GFP to surface puncta concentrated at the tips of mating projections. These puncta failed to form in cells defective in either the Gα or β subunits. However, they still formed in response to pheromone in cells missing Ste11, but with the puncta distributed around the cell periphery in the absence of mating projections. These puncta were absent from hst7Δ/Δ cells, but could be detected in the ste11Δ/Δ hst7Δ/Δ double mutant. Cek2-GFP showed a strong nuclear localization late in the response, consistent with a role in adaptation, while Cek1-GFP showed a weaker, but early increase in nuclear localization after pheromone treatment. Activation loop phosphorylation of both Cek1 and Cek2 required the presence of Ste11. In contrast to Cek2-GFP, which showed no localization signal in ste11Δ/Δ cells, Cek1-GFP showed enhanced nuclear localization that was pheromone independent in the ste11Δ/Δ mutant. The results are consistent with CaSte11 facilitating Hst7-mediated MAP kinase phosphorylation and also playing a potentially critical role in both MAP kinase and Cst5 scaffold localization.

10.1093/g3journal/jkaa033

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Antifungal activity of Cymbopogon citratus essential oil on Candida albicans and Candida tropicalis strains isolated from nosocomial infections

Revista do Instituto Adolfo Lutz (Impresso) (2009)

Fernando de Sá Silva Tatiane Morais Ferreira Guilherme Rodrigues Teodoro Anna Carolina Borges Pereira Costa Aguida Maria

Cymbopogon citratus

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Effect of photodynamic therapy on clinical isolates of Staphylococcus spp

Brazilian Oral Research (2011)

Michelle Miyabe Juliana Campos Junqueira Anna Carolina Borges Pereira Costa Antônio Olavo Cardoso Jorge Martha S. Ribeiro

Staphylococcus spp. are opportunistic microorganisms known for their capacity to develop resistance against antimicrobial agents. The objective of this study was to evaluate the effect of photodynamic therapy (PDT) on 20 Staphylococcus strains isolated from the human oral cavity, including S. aureus, S. schleiferi, S. epidermidis, S. capitis, S. haemolyticus, and S. lentus. A suspension of each Staphylococcus strain (10(6) cells/mL) was submitted to PDT using methylene blue and a low power laser. The isolated effects of methylene blue, laser treatment and ciprofloxacin were also evaluated. After the experimental treatments, 0.1 mL aliquots of the suspensions were seeded onto BHI agar for determination of the number of colony-forming units (CFU/mL). The results were analyzed by analysis of variance and Tukey's test (p < 0.05). The mean reduction in bacterial counts of the strains submitted to PDT ranged from 4.89 to 6.83 CFU (log10)/mL, with the observation of a decreasing susceptibility to treatment of S. schleiferi, S. haemolyticus, S. epidermidis, S. capitis, S. aureus, and S. lentus. The results showed that PDT was effective in reducing the number of viable cells of all clinical Staphylococcus isolates studied.

Methylene blue

Staphylococcus haemolyticus

Colony-forming unit

10.1590/s1806-83242011005000006

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Citations (45)

Susceptibility of Candida albicans, Staphylococcus aureus, and Streptococcus mutans biofilms to photodynamic inactivation: an in vitro study

Lasers in Medical Science (2010)

Cristiane Aparecida Pereira Rogério Lima Romeiro Anna Carolina Borges Pereira Costa Ana Karina Silva Machado Juliana Campos Junqueira

Methylene blue

Brain heart infusion

Crystal violet

Microtiter plate

Serial dilution

Colony-forming unit

10.1007/s10103-010-0852-3

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Citations (233)

Atividade antifúngica do óleo essencial de Melaleuca alternifolia sobre leveduras isoladas de candidíase bucal de gestantes HIV positivas

REVISTA DO INSTITUTO ADOLFO LUTZ (2010)

Anna Carolina Borges Pereira Costa Guilherme Rodrigues Teodoro Tatiane Morais Ferreira Fernando de Sá Silva Aguida Maria

A candidíase bucal é a infecção fúngica mais comum em portadores de HIV e, com episódios recorrentes em pacientes com Aids. Com o objetivo de pesquisar novos e eficazes agentes antifúngicos contra cepas resistentes, foi investigada a atividade antifúngica do óleo essencial de Melaleuca alternifolia, em diferentes concentrações, em leveduras isoladas de candidíase oral. O experimento foi realizado por meio da técnica de difusão em ágar. Foram avaliadas cepas padrão de Candida albicans ATCC 10231, Candida tropicalis ATCC 157, Candida glabrata ATCC 30070, Candida krusei ATCC 6258 e Candida dubliniensis ATCC 778157 e os isolados da cavidade bucal de gestantes HIV positivas, sendo sete C. albicans, um C. tropicalis, um C.glabrata e um C. krusei. O óleo essencial foi analisado nas quantidades de 20 e 50 μL, nas concentrações de 10 a 100%, variando de 10 em 10%. Todas as cepas analisadas foram suscetíveis ao óleo essencial de M. alternifolia nas concentrações de 70% e 50%, respectivamente, nos volumes de 20 μL e 50 μL. O potencial antifúngico do óleo essencial de M. alternifolia desperta interesse para o desenvolvimento de novos fármacos.

Candida krusei

Candida glabrata

Candida dubliniensis

10.53393/rial.2010.v69.32643

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Citations (4)