Walter L. Binder

Nova Medical (United States)

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Gary L. Norman

North Carolina State University

Ernst H. Beutner

University at Buffalo, State University of New York

Zakera Shums

Nova Medical (United States)

Stefania Jabłońska

Medical University of Warsaw

Anǵela Tincani

University of Brescia

Laura Andréoli

University of Brescia

Tadej Avčin

Ljubljana University Medical Centre

Pier Luigi Meroni

University of Milan

Cecilia Nalli

University of Brescia

Mario Motta

Azienda USL di Bologna

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Nova Medical (United States)

University at Buffalo, State University of New York

Hokkaido University

Azienda Socio Sanitaria Territoriale degli Spedali Civili di Brescia

Center for Rheumatology

University of Brescia

McMaster University

The University of Tokyo

Universidad del Salvador

Medical Research Council

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Standardized measurement of major immunoglobulin class (IgG, IgA, and IgM) antibodies to β2glycoprotein I in patients with antiphospholipid syndrome

Journal of Clinical Laboratory Analysis (1998)

Stephen J. Lewis L B Keil Walter L. Binder Vincent A. DeBari

Patients with the antiphospholipid syndrome (APS) have autoantibodies directed against epitopes on β2 glycoprotein I (β2GPI). We describe herein the performance characteristics of standardized enzyme-linked immunosorbent assays (ELISAs) for anti-β2GPI of the three major immunoglobulin classes: IgG, IgA, and IgM. All three assays generated highly linear standard curves (5 points, r ≥ 0.993 for each); precision was excellent both intra-assay and run-to-run, with coefficients of variation (CV) ranging from 2.3% to 6.6%. Values for IgG anti-β2GPI correlated strongly with those obtained by an earlier method (r = 0.80, P < 0.0001). A study group consisting of 203 healthy subjects was used to generate percentile-based reference intervals for all three classes of anti-β2GPI. APS subjects' anti-β2GPI were found to differ significantly (P < 0.0001 for each) for those of the control population. All three assays correlated well with β2GPI-dependent anticardiolipin antibody (aCL) measurements; IgG: r = 0.94 (P < 0.0001), for IgA: r = 0.82 (P < 0.001) and for IgM: r = 0.84 (P < 0.0001). We suggest that these ELISAs may provide valuable standardized measurements of IgG, IgA, and IgM anti-β2GPI. J. Clin. Lab. Ana. 12:293–297, 1998. © 1998 Wiley-Liss, Inc.

Immunoglobulin A

Immunoglobulin M

10.1002/(sici)1098-2825(1998)12:5<293::aid-jcla8>3.0.co;2-7

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IgG and IgA anti-Saccharomyces cerevisiae antibodies (ASCA) detection by ELISA in Crohn's disease, ulcerative colitis and healthy patient groups

Gastroenterology (2000)

Gary L. Norman Zakera Gandhi Tiffany M. Crowther Walter L. Binder Verena Kratzer

10.1016/s0016-5085(00)82499-3

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Citations (3)