Pavlína Novotná

University of Chemistry and Technology, Prague

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Marie Urbanová

University of Chemistry and Technology, Prague

Václav Kašička

Czech Academy of Sciences, Institute of Organic Chemistry and Biochemistry

DŠ

David Šaman

Czech Academy of Sciences, Institute of Organic Chemistry and Biochemistry

Dušan Koval

Czech Academy of Sciences, Institute of Organic Chemistry and Biochemistry

Petra Sázelová

Czech Academy of Sciences, Institute of Organic Chemistry and Biochemistry

Filip Teplý

Czech Academy of Sciences, Institute of Organic Chemistry and Biochemistry

Lukáš Severa

Czech Academy of Sciences, Institute of Organic Chemistry and Biochemistry

Ivana Cı́sařová

Charles University

Petr Slavı́ček

University of Chemistry and Technology, Prague

Radek Pohl

Czech Academy of Sciences, Institute of Organic Chemistry and Biochemistry

Cooperative Institutions

Charles University

Czech Academy of Sciences, Institute of Organic Chemistry and Biochemistry

Czech Academy of Sciences

University of Chemistry and Technology, Prague

Czech Academy of Sciences, Institute of Physics

University of Hradec Králové

Czech Academy of Sciences, J. Heyrovský Institute of Physical Chemistry

TU Dresden

Centre National de la Recherche Scientifique

Czech Academy of Sciences, Institute of Physiology

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Functional helquats: helical cationic dyes with marked, switchable chiroptical properties in the visible region

Chemical Communications (2014)

Paul E. Reyes‐Gutiérrez Michael Jirásek Lukáš Severa Pavlína Novotná Dušan Koval

Helically chiral styryl dyes with large ECD in the visible region are introduced.

Helicene

Cationic polymerization

Chromophore

Visible spectrum

10.1039/c4cc08967g

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Citations (50)

Vibrational circular dichroism study of polypeptide model–membrane systems

Analytical Biochemistry (2012)

Pavlína Novotná Marie Urbanová

Sodium dodecyl sulfate

Polylysine

10.1016/j.ab.2012.03.023

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Citations (21)

CCDC 925830: Experimental Crystal Structure Determination

The Cambridge Structural Database (2015)

Paul E. Reyes‐Gutiérrez Michael Jirásek Lukáš Severa Pavlína Novotná Dušan Koval

An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.

Crystal (programming language)

10.5517/cc102dhz

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Citations (0)

Resolution of a configurationally stable [5]helquat: enantiocomposition analysis of a helicene congener by capillary electrophoresis

New Journal of Chemistry (2010)

Lukáš Severa Dušan Koval Pavlína Novotná Milan Ončák Petra Sázelová

Racemic [5]helquat as a triflate salt has been synthesized using a robust, three-step procedure. Subsequent exchange of triflate anions for inexpensive (R,R)-dibenzoyltartrate anions via an ion exchange resin afforded two diastereoisomeric salts. Crystallization led to the resolution of the helquat (ee > 98%). This is the first time that a non-racemic helquat has been obtained; its helicity having been assigned and its racemization barrier determined. Capillary electrophoresis with a sulfated β-cyclodextrin chiral selector is introduced for the first time as a straightforward method to analyze the enantiocomposition of charged, helicene-like species.

Helicene

Racemization

Trifluoromethanesulfonate

10.1039/c0nj00085j

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Citations (35)

CCDC 925828: Experimental Crystal Structure Determination

The Cambridge Structural Database (2015)

Paul E. Reyes‐Gutiérrez Michael Jirásek Lukáš Severa Pavlína Novotná Dušan Koval

10.5517/cc102dfx

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Citations (0)

CCDC 1004340: Experimental Crystal Structure Determination

The Cambridge Structural Database (2015)

Paul E. Reyes‐Gutiérrez Michael Jirásek Lukáš Severa Pavlína Novotná Dušan Koval

10.5517/cc12q32z

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The C-Terminal Segment of Yeast BMH Proteins Exhibits Different Structure Compared to Other 14-3-3 Protein Isoforms

Biochemistry (2010)

Dana Veisova Lenka Řežábková Miroslav Štěpánek Pavlína Novotná Petr Heřman

Yeast 14-3-3 protein isoforms BMH1 and BMH2 possess a distinctly variant C-terminal tail which differentiates them from the isoforms of higher eukaryotes. Their C-termini are longer and contain a polyglutamine stretch of unknown function. It is now well established that the C-terminal segment of 14-3-3 proteins plays an important regulatory role by functioning as an autoinhibitor which occupies the ligand binding groove and blocks the binding of inappropriate ligands. Whether the same holds true or not for the yeast isoforms is unclear. Therefore, we investigated the conformational behavior of the C-terminal segment of BMH proteins using various biophysical techniques. Dynamic light scattering, sedimentation velocity, time-resolved fluorescence anisotropy decay, and size exclusion chromatography measurements showed that the molecules of BMH proteins are significantly larger compared to the human 14-3-3zeta isoform. On the other hand, the sedimentation analysis confirmed that BMH proteins form dimers. Time-resolved tryptophan fluorescence experiments revealed no dramatic structural changes of the C-terminal segment upon the ligand binding. Taken together, the C-terminal segment of BMH proteins adopts a widely opened and extended conformation that makes difficult its folding into the ligand binding groove, thus increasing the apparent molecular size. It seems, therefore, that the C-terminal segment of BMH proteins does not function as an autoinhibitor.

10.1021/bi100273k

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Citations (28)

Cyclopeptides containing the DEKS motif as conformationally restricted collagen telopeptide analogues: synthesis and conformational analysis

Organic & Biomolecular Chemistry (2014)

Robert Wodtke Gloria Ruiz‐Gómez Manuela Kuchar M. Teresa Pisabarro Pavlína Novotná

The conformational stabilisation of the DEKS motif at the α1(I) collagenN-telopeptide within a cyclic hexapeptide together with its conformational analysis is described. This sequence is critical for lysyl oxidase-mediated collagen crosslinking, a process which is deregulated during tumour progression.

Cyclic peptide

Side chain

Lysyl Oxidase

Residue (chemistry)

10.1039/c4ob02348j

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Citations (11)

Isoquinoline Alkaloids from Fumaria officinalis L. and Their Biological Activities Related to Alzheimer's Disease

Chemistry & Biodiversity (2016)

Jakub Chlebek Zdeněk Novák Dominika Kassemová Marcela Šafratová Jan Kostelník

Two new isoquinoline alkaloids, named fumaranine (2) and fumarostrejdine (10), along with 18 known alkaloids were isolated from aerial parts of Fumaria officinalis. The structures of the isolated compounds were elucidated on the basis of spectroscopic analyses and by comparison with literature data. The absolute configuration of the new compound 2 was determined by comparing its circular dichroism spectra with those of known analogs. Compounds isolated in sufficient amounts were evaluated for their acetylcholinesterase, butyrylcholinesterase, prolyl oligopeptidase (POP), and glycogen synthase kinase-3β inhibitory activities. Parfumidine (8) and sinactine (15) exhibited potent POP inhibition activities (IC50 99±5 and 53±2 μM, resp.).

Isoquinoline

Butyrylcholinesterase

Benzylisoquinoline

Oligopeptidase

10.1002/cbdv.201500033

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Citations (36)

Conformational study of melectin and antapin antimicrobial peptides in model membrane environments

Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy (2016)

Lucie Kocourková Pavlína Novotná Sabína Čujová Václav Čeřovský Marie Urbanová

10.1016/j.saa.2016.07.015

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Citations (20)