Abstract Several cytokines have been found to induce human peripheral blood lymphocyte (PBL) migration in vitro . The mechanisms involved are unclear, therefore experiments were carried out to determine whether PBL migration in response to selected cytokines is due to a direct effect, or to the generation of nonspecific secondary mediators, and whether migration is receptor specific. Purified human PBL were incubated with the lymphocyte chemotactic cytokines interleukin (IL)‐1α and IL‐8, followed after 30‐‐60 min by addition of specific neutralizing monoclonal antibody. Supernatants of these mixtures were shown by subsequent assay to be devoid of PBL attractant activity, whereas positive control supernatants containing no antibody induced dilution‐related migration. Addition of antibody to the high affinity IL‐2 receptor abolished the potent attractant effect of IL‐2 on PBL, but had little effect on responses to IL‐1α and IL‐8. These results demonstrate that the in vitro locomotor responses of human PBL to selected cytokines are due to direct, receptor‐specific effects and are not dependent upon the generation of secondary mediator(s).
Journal Article Skin exudate levels of interleukin 6, interleukin i and other cytokines in mycosis fungoides Get access FRANCES LAWLOR, FRANCES LAWLOR Institute of Dermatology, St Thomas's Hospital, London Dr Frances Lawlor, Institute of Dermatology, St Thomas's Hospital, Lambeth Palace Road, London SE17EH, U.K. Search for other works by this author on: Oxford Academic Google Scholar N.P. SMITH, N.P. SMITH Skin Tumour Unit, St John's Hospital for Diseases of the Skin, London Search for other works by this author on: Oxford Academic Google Scholar R.D.R. CAMP, R.D.R. CAMP Institute of Dermatology, St Thomas's Hospital, London Search for other works by this author on: Oxford Academic Google Scholar K.B. BACON, K.B. BACON Institute of Dermatology, St Thomas's Hospital, London Search for other works by this author on: Oxford Academic Google Scholar ANNE KOBZA BLACK, ANNE KOBZA BLACK Institute of Dermatology, St Thomas's Hospital, London Search for other works by this author on: Oxford Academic Google Scholar M.W. GREAVES, M.W. GREAVES Institute of Dermatology, St Thomas's Hospital, London Search for other works by this author on: Oxford Academic Google Scholar A.J.H. GEARING A.J.H. GEARING National Institute for Biological Standards and Control, South Mimms, Hertfordshire, U.K. Search for other works by this author on: Oxford Academic Google Scholar British Journal of Dermatology, Volume 123, Issue 3, 1 September 1990, Pages 297–304, https://doi.org/10.1111/j.1365-2133.1990.tb06288.x Published: 01 September 1990
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