Hiromitsu Inoue

National Agriculture and Food Research Organization

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Trends

Author Order

Document Type

Co-Authors

Atsushi Nakabachi

Toyohashi University of Technology

Yuu Hirose

Toyohashi University of Technology

Geonho Cho

Sunchon National University

Tomonori Arai

National Agriculture and Food Research Organization

Daniel Burckhardt

Natural History Museum of Basel

Xinyu Luo

China Agricultural University

Toru Iwanami

Tokyo University of Agriculture

Seunghwan Lee

Seoul National University

Shigeki Kishi

National Ready Mixed Concrete Association

Hiroshi Katoh

Takasaki University of Health and Welfare

Cooperative Institutions

National Agriculture and Food Research Organization

Institute of Fruit Tree and Tea Science

The University of Tokyo

Kyoto University

Kyushu University

University of Tsukuba

Hokkaido University

Toyohashi University of Technology

National Institute for Agro-Environmental Sciences

University of Bonn

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Field

Unique Features of a Japanese ‘Candidatus Liberibacter asiaticus’ Strain Revealed by Whole Genome Sequencing

PLoS ONE (2014)

Hiroshi Katoh Shin-ichi Miyata Hiromitsu Inoue Toru Iwanami

Citrus greening (huanglongbing) is the most destructive disease of citrus worldwide. It is spread by citrus psyllids and is associated with phloem-limited bacteria of three species of α-Proteobacteria, namely, 'Candidatus Liberibacter asiaticus', 'Ca. L. americanus', and 'Ca. L. africanus'. Recent findings suggested that some Japanese strains lack the bacteriophage-type DNA polymerase region (DNA pol), in contrast to the Floridian psy62 strain. The whole genome sequence of the pol-negative 'Ca. L. asiaticus' Japanese isolate Ishi-1 was determined by metagenomic analysis of DNA extracted from 'Ca. L. asiaticus'-infected psyllids and leaf midribs. The 1.19-Mb genome has an average 36.32% GC content. Annotation revealed 13 operons encoding rRNA and 44 tRNA genes, but no typical bacterial pathogenesis-related genes were located within the genome, similar to the Floridian psy62 and Chinese gxpsy. In contrast to other 'Ca. L. asiaticus' strains, the genome of the Japanese Ishi-1 strain lacks a prophage-related region.

Prophage

GC-content

Genome size

Strain (injury)

10.1371/journal.pone.0106109

Cite

Citations (61)

DNA barcoding and adult morphology reveal an unrecorded species on Citrus and other new host associations of Blastobasidae (Lepidoptera: Gelechioidea) in Japan, with taxonomic notes on the genus Lateantenna

Lepidoptera Science (2018)

Issei Ohshima Yositaka Sakamaki Hiromitsu Inoue Tomonori Arai David Adamski

DNA Barcoding

Morphology

10.18984/lepid.69.1_1

Cite

Citations (1)

Changes in Variable Number of Tandem Repeats in ‘Candidatus Liberibacter asiaticus’ through Insect Transmission

PLoS ONE (2015)

Hiroshi Katoh Hiromitsu Inoue Toru Iwanami

Citrus greening (huanglongbing) is the most destructive citrus disease worldwide. The disease is associated with three species of 'Candidatus Liberibacter' among which 'Ca. Liberibacter asiaticus' has the widest distribution. 'Ca. L. asiaticus' is commonly transmitted by a phloem-feeding insect vector, the Asian citrus psyllid Diaphorina citri. A previous study showed that isolates of 'Ca. L. asiaticus' were clearly differentiated by variable number of tandem repeat (VNTR) profiles at four loci in the genome. In this study, the VNTR analysis was further validated by assessing the stability of these repeats after multiplication of the pathogen upon host-to-host transmission using a 'Ca. L. asiaticus' strain from Japan. The results showed that some tandem repeats showed detectable changes after insect transmission. To our knowledge, this is the first report to demonstrate that the repeat numbers VNTR 002 and 077 of 'Ca. L. asiaticus' change through psyllid transmission. VNTRs in the recipient plant were apparently unrelated to the growing phase of the vector. In contrast, changes in the number of tandem repeats increased with longer acquisition and inoculation access periods, whereas changes were not observed through psyllid transmission after relatively short acquisition and inoculation access periods, up to 20 and 19 days, respectively.

Diaphorina citri

10.1371/journal.pone.0138699

Cite

Citations (10)

Systematics of the east Palaearctic pear psyllids (Hemiptera: Psylloidea) with particular focus on the Japanese and Korean fauna

Zootaxa (2017)

Geonho Cho Daniel Burckhardt Hiromitsu Inoue Xinyu Luo Seunghwan Lee

The confused taxonomy of the east Palaearctic pear psyllids, serious pests on cultivated pear, is reviewed. Fifty-six nominal species have been reported from Pyrus, 25 of which we consider valid and ten as not being associated with Pyrus. Our taxonomic revision suggests that, in Korea, four Cacopsylla species develop on pear: the univoltine C. burckhardti Luo et al. previously misidentified as C. pyrisuga (Foerster), the polyvoltine, seasonally dimorphic C. jukyungi (Kwon) (winter form ‘cinereosignata’ Luo et al., summer form ‘jukyungi’), commonly found in Korean pear orchards, and C. maculatili Li (winter form ‘maculatili’, summer form ‘qiuzili’ Li) previously misidentified as C. pyricola (Foerster) by some authors, as well as the probably polyvoltine but not dimorphic C. sandolbaea (Park & Lee). The former three species (C. burckhardti, C. jukyungi, misidentified as C. chinensis (Yang & Li), and C. maculatili) occur also in Japan. Keys to the adult and fifth instar immatures as well as short biological notes are provided, and C. jukyungi and C. sandolbaea are redescribed. Following nomenclatorial changes are proposed: Cacopsylla betulaefoliae (Yang & Li, 1981) = Psylla heterobetulaefoliae Yang & Li, 1981, syn. nov.; Cacopsylla bidens (Šulc, 1907) = Psylla jiangli Yang & Li, 1981, syn. nov.; Cacopsylla jukyungi (Kwon) = C. cinereosignata Luo et al., syn. nov.; Cacopsylla maculatili Li = C. qiuzili Li, syn. nov.; Cacopsylla nigella (Konovalova), comb. nov. from Psylla. The synonymy of P. obongsana Kwon with C. sandolbaea is confirmed.

Psylloidea

Pyrus communis

Bionomics

10.11646/zootaxa.4362.1.4

Cite

Citations (97)

High-resolution Microbiome Analyses of Nine Psyllid Species of the Family Triozidae Identified Previously Unrecognized but Major Bacterial Populations, including <i>Liberibacter</i> and <i>Wolbachia</i> of Supergroup O

Microbes and Environments (2022)

Atsushi Nakabachi Hiromitsu Inoue Yuu Hirose

Psyllids (Hemiptera: Sternorrhyncha: Psylloidea) are plant sap-sucking insects that include important agricultural pests. To obtain insights into the ecological and evolutionary behaviors of microbes, including plant pathogens, in Psylloidea, high-resolution ana-lyses of the microbiomes of nine psyllid species belonging to the family Triozidae were performed using high-throughput amplicon sequencing of the 16S rRNA gene. Analyses identified various bacterial populations, showing that all nine psyllids have at least one secondary symbiont, along with the primary symbiont "Candidatus Carsonella ruddii" (Gammaproteobacteria: Oceanospirillales: Halomonadaceae). The majority of the secondary symbionts were gammaproteobacteria, particularly those of the order Enterobacterales, which included Arsenophonus and Serratia symbiotica, a bacterium formerly recognized only as a secondary symbiont of aphids (Hemiptera: Sternorrhyncha: Aphidoidea). The non-Enterobacterales gammaproteobacteria identified in the present study were Diplorickettsia (Diplorickettsiales: Diplorickettsiaceae), a potential human pathogen, and Carnimonas (Oceanospirillales: Halomonadaceae), a lineage detected for the first time in Psylloidea. Regarding alphaproteobacteria, the potential plant pathogen "Ca. Liberibacter europaeus" (Rhizobiales: Rhizobiaceae) was detected for the first time in Epitrioza yasumatsui, which feeds on the Japanese silverberry Elaeagnus umbellata (Elaeagnaceae), an aggressive invasive plant in the United States and Europe. Besides the detection of Wolbachia (Rickettsiales: Anaplasmataceae) of supergroup B in three psyllid species, a lineage belonging to supergroup O was identified for the first time in Psylloidea. These results suggest the rampant transfer of bacterial symbionts among animals and plants, thereby providing deeper insights into the evolution of interkingdom interactions among multicellular organisms and bacteria, which will facilitate the control of pest psyllids.

Psylloidea

Candidatus

Sternorrhyncha

10.1264/jsme2.me22078

Cite

Citations (10)

Occurrence of Psylla evodiae Miyatake (Hemiptera: Psyllidae) on the Cultivated Orange Jasmine, Murraya paniculata (Rutaceae) in Kyushu and on Yakushima Island.

Japanese Journal of Applied Entomology and Zoology (2006)

Hiromitsu Inoue Kazutaka Shinohara M. Okumura Kousuke Ikeda Wataru Ashihara

The occurrence of Psylla evodiae Miyatake, which has been known to occur on Euodia meliifolia (Rutaceae) in the Yaeyama Islands of Japan and in Taiwan, has been newly recorded in Kyushu (Fukuoka, Nagasaki, and Kagoshima prefectures) and on Yakushima Island and Okinawa Island in southwestern Japan. In addition, it has been confirmed that P. evodiae feeds on Murraya paniculata (Rutaceae), which is a newly recorded host plant in southern Kyushu (Kagoshima Prefecture) and Yakushima Island. It has also been confirmed that P. evodiae feeds on Zanthoxylum beecheyanum var. alatum (Rutaceae) in Okinawa Island.

Murraya

Zanthoxylum

10.1303/jjaez.2006.66

Cite

Citations (2)

FIGURES 45−50 in Systematics of the east Palaearctic pear psyllids (Hemiptera: Psylloidea) with particular focus on the Japanese and Korean fauna

Geonho Cho Daniel Burckhardt Hiromitsu Inoue Xinyu Luo Seung Hwan Lee

Psylloidea

10.5281/zenodo.1076278

Cite

Citations (0)

Descriptions of Two New Species of the Genus Cacopsylla (Hemiptera : Psyllidae) in Japan

ESAKIA (2004)

Hiromitsu Inoue

Two new species of the genus Cacopsylla are described from Japan.One of them, Cacopsylla swidae sp.n. feeding on Swida controversa (Cornaceae), is described based on adults and 5th instars.Cacopsylla swidae became clear to be identical with the Japanese species that had been erroneously identified as a European species, Cacopsylla melanoneura.As a result, Cacopsylla melanoneura is excluded from the psyllid fauna of Japan.Another new species, Cacopsylla yukawai sp.n., which is distinguished from C. swidae and C. melanoneura in the shapes of genal cones and genitalia of both sexes, is described based on adults.

10.5109/2690

Cite

Citations (1)

Sex Pheromone of the Azalea Mealybug: Absolute Configuration and Kairomonal Activity

Journal of Chemical Ecology (2024)

Yuma Sugawara Hiromitsu Inoue Satoshi Toda Jun Tabata

Mealybug

Absolute Configuration

10.1007/s10886-024-01473-2

Cite

Citations (2)

First fossil record of a jumping plant-louse (Insecta, Hemiptera, Psylloidea) from Japan