Danchao Du

Citrus Research Institute

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Lianming Lu

Citrus Research Institute

Zhanxu Pu

Citrus Research Institute

Xiurong Hu

Citrus Research Institute

Zhendong Huang

Nanjing University of Posts and Telecommunications

Baoping Cheng

Guangdong Academy of Agricultural Sciences

Guoqing Chen

Xuzhou Medical College

Aitian Peng

Guangdong Academy of Agricultural Sciences

Shunmin Liu

Citrus Research Institute

Lyu Jia

Citrus Research Institute

Li Zhu

First Affiliated Hospital of Kunming Medical University

Cooperative Institutions

Citrus Research Institute

Institute of Plant Protection

Ministry of Agriculture and Rural Affairs

Guangdong Academy of Agricultural Sciences

Chinese Academy of Agricultural Sciences

Nanjing University of Posts and Telecommunications

The Synergetic Innovation Center for Advanced Materials

Nanjing Medical University

Huazhong Agricultural University

Ningxia Medical University

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First Report of Cladosporium perangustum Causing Leaf Spot of Myrica rubra in China

Plant Disease (2014)

Lianming Lu Baoping Cheng Zhanxu Pu Xueping Hu Danchao Du

Myrica rubra

Cladosporium

10.1094/pdis-10-14-1080-pdn

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Citations (2)

Characterization of the complete chloroplast genome of Satsuma mandarin (Citrus unshiu) in China

Research Square (Research Square) (2022)

Danchao Du Zhu Li Zhanxu Pu Lianming Lu Zhendong Huang

Abstract Satsuma mandarin is one of the most widely cultivated citrus in the world. In this study, we firstly presented the complete chloroplast genome of Satsuma mandarin by using BGISEQ-500 sequencing technology. The complete chloroplast genome of Satsuma mandarin is 160,699 bp in size, including a pair of inverted repeat regions of 26,990 bp, a large single copy region of 87,918 bp, and a small single copy region of 18,801 bp. The complete chloroplast genome of Satsuma mandarin contains 108 unique genes, including 75 protein coding genes, 29 tRNA genes and 4 rRNA genes. Phylogenetic analysis based on the complete chloroplast genome showed that the relationship between Satsuma mandarin and citrus reticulate was the closest.

Citrus unshiu

Inverted repeat

Mandarin Chinese

10.21203/rs.3.rs-1663368/v1

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A novel TaqMan qPCR system for the detection of Diaporthe citri , the causative agent of citrus melanose

Biotechnology & Biotechnological Equipment (2024)

Zhanxu Pu Feng Huang Li Zhu Danchao Du Xiurong Hu

Citrus melanose, caused by Diaporthe citri, is one of the most economically significant fungal diseases in China. Given that the application of fungicides is the main technique used for the control of citrus melanose, many studies have focused on enhancing the effectiveness and efficiency of the use of fungicides for the control of this economically significant disease. Here, we developed a TaqMan quantitative polymerase chain reaction (qPCR) protocol for monitoring citrus melanose via detection of suspected infected leaves and airborne conidia of D. citri. A primer pair and TaqMan probe targeting D. citri were designed to amplify a region of the fungal β-tubulin gene. The limits of detection and quantification of our TaqMan qPCR method were 159.57 and 683 copies/μL, respectively. The method detected the DNA equivalent to 47 conidia when fungal conidia were cultured in vitro. To evaluate the feasibility of our TaqMan qPCR method, symptomatic and asymptomatic leaf samples, airborne conidia samples were collected from a citrus orchard and tea garden, respectively. D. citri was detected in leaves with citrus melanose symptoms in an orchard; airborne fungal conidia of D. citri were also detected. These findings suggested that this novel TaqMan qPCR method could be used to detect D. citri both in vivo and in vitro. Our methodology could be applied as an alternative method for monitoring the changes in the incidence of D. citri in orchards.

TaqMan

10.1080/13102818.2024.2338437

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Identification ofPestalotiopsis clavisporacausing brown leaf spot on Chinese bayberry in China

Canadian Journal of Plant Pathology (2015)

Lianming Lu Guoqing Chen Xiurong Hu Danchao Du Zhanxu Pu

A brown leaf spot disease was observed on Chinese bayberry (Myrica rubra) in Zhejiang Province, China during 2012 and 2014. A fungus was isolated from diseased leaves showing typical symptoms of brown leaf spot. The pathogenicity of the isolated fungus was confirmed through inoculation experiments. Based on morphological characteristics and molecular analyses, the pathogen was identified as Pestalotiopsis clavispora (G. F. Atk.) Steyaert. This is the first report of P. clavispora causing brown leaf spot on Chinese bayberry in China.

Pestalotiopsis

Myrica rubra

Spots

10.1080/07060661.2015.1065001

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Citations (10)

Virulence of Purpureocillium lilacinum strain ZJPL08 and efficacy of a wettable powder formulation against the Asian citrus psyllid (Diaphorina citri)

Biotechnology & Biotechnological Equipment (2020)

Danchao Du Lianming Lu Xiurong Hu Zhanxu Pu Zhendong Huang

(2020). Virulence of Purpureocillium lilacinum strain ZJPL08 and efficacy of a wettable powder formulation against the Asian citrus psyllid (Diaphorina citri) Biotechnology & Biotechnological Equipment: Vol. 34, No. 1, pp. 1104-1113.

Diaphorina citri

Wettable powder

Strain (injury)

10.1080/13102818.2020.1823881

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Citations (10)

Morphological, molecular and virulence characterization of three Lencanicillium species infecting Asian citrus psyllids in Huangyan citrus groves

Journal of Invertebrate Pathology (2015)

Lianming Lu Baoping Cheng Danchao Du Xiurong Hu Aitian Peng

Diaphorina citri

Hypocreales

10.1016/j.jip.2015.01.002

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Citations (32)

A New Diagnostic System for Detection of ‘Candidatus Liberibacter asiaticus’ Infection in Citrus

Plant Disease (2013)

Lianming Lu Baoping Cheng Jinai Yao Aitian Peng Danchao Du

In this study, two polyclonal antibodies were produced against the Omp protein of 'Candidatus Liberibacter asiaticus'. First, omp genes were sequenced to exhibit 99.9% identity among 137 isolates collected from different geographical origins. Then, two peptides containing the hydrophobic polypeptide-transport-associated (POTRA) domain and β-barrel domain, respectively, were identified on Omp protein. After that, these two peptides were overexpressed in Escherichia coli and purified by affinity chromatography to immunize the white rabbits. Finally, the antiserum was purified by affinity chromatography. The two Omp antibodies gave positive results (0.454 to 0.633, 1:1,600 dilution) in enzyme-linked immunosorbent assay against 'Ca. L. asiaticus'-infected samples collected from different geographical origins but revealed negative results against other pathogen-infected, nutrient-deficient and healthy samples. The antibody against the POTRA domain of Omp protein could detect 'Ca. L. asiaticus' in 45.7% of the symptomatic samples compared with a 56.2% detection rate with a polymerase chain reaction assay. These new antibodies will provide a very useful supplement to the current approaches to 'Ca. L. asiaticus' detection and also provide powerful research tools for tracking distribution of this pathogen in vivo.

Diaphorina citri

10.1094/pdis-11-12-1086-re

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Citations (10)

Isolation and identification of a marine actinomycete strain and its control efficacy against citrus green and blue moulds

Biotechnology & Biotechnological Equipment (2019)

Xiurong Hu Baoping Cheng Danchao Du Zhendong Huang Zhanxu Pu

The most serious citrus postharvest diseases are citrus green mould caused by Penicillium digitatum and citrus blue mould caused by Penicillium italicum. Marine microorganisms have become a new measure in the control of these diseases. In this study, we isolated 56 marine actinomycetes, and strain AM-4 isolated from Drupa granulata exhibited the strongest antagonistic activity against P. digitatum. Molecular, morphological, cultural and physio-biochemical analysis identified strain AM-4 as Streptomyces chumphonensis. Strain AM-4 also exhibited antagonistic activity against 11 other phytopathogens, with an inhibition zone width ranging from 0.65 ± 0.04 to 2.35 ± 0.02 cm. The mycelial growth inhibition rates of the fermentation liquid of strain AM-4 against P. digitatum and P. italicum were 66.23% and 61.42%, respectively, and the conidial germination inhibition rates were 65.77% and 62.13%, respectively. The relative control efficacy of the gradient-diluted fermentation extracts of strain AM-4 against citrus green mould caused by P. digitatum ranged from 77.95% to 92.91% at 5 days post inoculation and 56.30% to 87.22% 10 days after inoculation. The relative control efficacy of the gradient diluted fermentation extracts of strain AM-4 against citrus blue mould caused by P. italicum ranged from 61.32% to 84.91% 5 days after inoculation and from 51.34% to 80.92% 10 days after inoculation. The relative control efficacy of the fermentation extracts of strain AM-4 was even better than prochloraz against citrus green mould caused by prochloraz-resistant P. digitatum. Therefore, as a biological control strain, AM-4 has a good prospect for development and utilization potential.

Penicillium digitatum

Penicillium italicum

Strain (injury)

Blue mold

Citrus × sinensis

10.1080/13102818.2019.1613175

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Citations (9)

Biocontrol of citrus fungal pathogens by lipopeptides produced by Bacillus velezensis TZ01

Frontiers in Microbiology (2024)

Baoju An Danchao Du Zhen‐Dong Huang Zhanxu Pu Jia Lv

Citrus diseases caused by fungal pathogens drastically decreased the yield and quality of citrus fruits, leading to huge economic losses. Given the threats of chemical pesticides on the environment and human health, biocontrol agents have received considerable attention worldwide as ecofriendly and sustainable alternative to chemical fungicides. In the present study, we isolated a

Bacillus (shape)

10.3389/fmicb.2024.1471305

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Citations (1)