Hartmut Beug

Research Institute of Molecular Pathology

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Frank Martin

Leipzig University

Martin Oft

Synthego (United States)

Jürgen Niessing

Philipps University of Marburg

M J Hayman

Stony Brook University

Roswitha Sgonc

Innsbruck Medical University

Susanne Meyer

University of California, Santa Barbara

Karl-Heinz Heider

FZI Research Center for Information Technology

Georg Wick

Innsbruck Medical University

Marianne Antoine

University of Regensburg

Peter Steinlein

Gregor Mendel Institute of Molecular Plant Biology

Cooperative Institutions

Philipps University of Marburg

Innsbruck Medical University

Universität Innsbruck

Austrian Academy of Sciences

University of California, Davis

Medizinische Hochschule Hannover

University Hospital Innsbruck

Leipzig University

Mathematica Policy Research

Brandenburg University of Technology Cottbus-Senftenberg

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Self-renewal and differentiation of normal avian erythroid progenitor cells: Regulatory roles of the TGFαc-ErbB and SCFc-Kit receptors

Cell (1993)

M J Hayman Susanne Meyer Frank Martin Peter Steinlein Hartmut Beug

Progenitor

10.1016/0092-8674(93)90303-8

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Citations (86)

Thymocyte apoptosis induced by elevated endogenous corticosterone levels

European Journal of Immunology (1994)

Johann Gruber Roswitha Sgonc Yan Hu Hartmut Beug Georg Wick

Abstract A well‐known model of apoptosis is induction in thymocytes by injection of pharmacological doses of exogenous steroids. The aim of this study was to investigate whether this process also occurs under physiological conditions, i.e. by stimulation of endogenous glucocorticoid release, using the chicken as an experimental model. Endogenous glucocorticoid levels can be elevated by immunization with exogenous antigens or by injection of conditioned medium, e.g. supernatant of mitogen‐stimulated spleen cells. This effect is mediated by so‐called glucocorticoid‐increasing factors, and is considered to act as an immunoregulatory principle. Thymocyte DNA of so treated birds showed a typical “ladder” pattern after electrophoresis in a 1.8% agarose gel, and degradation could be prevented by RU 38486. This provides evidence that apoptosis can be induced by elevating endogenous corticosterone levels in vivo. By means of in situ nick translation (ISNT) and simultaneous immunofluorescence tests, it was possible to analyze various thymic subpopulations during apoptosis after treatment with exogenous glucocorticoids. Additionally, using confocal microscopical techniques, apoptosis of the same cells as analyzed by ISNT is shown. The possible role of elevated concentrations of endogenous glucocorticoids in regulating thymocyte cell death and autoimmune diseases is discussed.

Thymocyte

Corticosterone

Agarose gel electrophoresis

10.1002/eji.1830240516

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Citations (127)

Primary structure and expression of a chicken cDNA encoding a protein with zinc-finger motifs

Gene (1991)

Andreas Benn Marianne Antoine Hartmut Beug Jürgen Niessing

Mesonephros

Zinc finger nuclease

Cloning (programming)