Abstract The maternal metabolic environment can be detrimental to the health of the offspring. In a previous work, we showed that maternal high-fat (HH) feeding in rabbit induced sex-dependent metabolic adaptation in the fetus and led to metabolic syndrome in adult offspring. As early development representing a critical window of susceptibility, in the present work we aimed to explore the effects of the HH diet on the oocyte, preimplantation embryo and its microenvironment. In oocytes from females on HH diet, transcriptomic analysis revealed a weak modification in the content of transcripts mainly involved in meiosis and translational control. The effect of maternal HH diet on the embryonic microenvironment was investigated by identifying the metabolite composition of uterine and embryonic fluids collected in vivo by biomicroscopy. Metabolomic analysis revealed differences in the HH uterine fluid surrounding the embryo, with increased pyruvate concentration. Within the blastocoelic fluid, metabolomic profiles showed decreased glucose and alanine concentrations. In addition, the blastocyst transcriptome showed under-expression of genes and pathways involved in lipid, glucose and amino acid transport and metabolism, most pronounced in female embryos. This work demonstrates that the maternal HH diet disrupts the in vivo composition of the embryonic microenvironment, where the presence of nutrients is increased. In contrast to this nutrient-rich environment, the embryo presents a decrease in nutrient sensing and metabolism suggesting a potential protective process. In addition, this work identifies a very early sex-specific response to the maternal HH diet, from the blastocyst stage.

10.1017/s2040174423000260

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Dynamics of DNA methylation levels in maternal and paternal rabbit genomes after fertilization

Epigenetics (2011)

Adriana R. Reis e Silva Pierre Adenot Nathalie Daniel Catherine Archilla Nathalie Peynot

The reprogramming of DNA methylation in early embryos has been considered to be essential for the reprogramming of differentiated parental genomes to totipotency, the transcription of embryonic genome activation (EGA) and subsequent development. However, its degree appears to differ as a function of species and it may be altered by the in vitro environment. While the rabbit is a pertinent model for species with a delayed EGA because both in vivo and in vitro developed embryos are easily available, the status of DNA methylation levels in both parental genomes after fertilization remains controversial. In order to generate precise data on the DNA methylation status in rabbit zygotes, we first of all defined five pronuclear (PN) stages during the first cell cycle and then classified in vivo and in vitro developed rabbit zygotes according to these PN stages. Using this classification we precisely quantified both methylated DNA and the total DNA content during the one cell stage. The quantification of methylated DNA, normalized for the total DNA content, showed that both pronuclei displayed distinct patterns of DNA methylation reprogramming. In the maternal pronucleus (MP) the methylation level remained constant throughout the one cell stage, thanks to maintenance methylation during the S phase. Conversely, in the paternal pronucleus (PP) partial demethylation occurred before replication, probably as a result of active DNA demethylation, while maintenance methylation subsequently occurred during the S phase. Interestingly, we showed that PP DNA methylation reprogramming was partially altered by the in vitro environment. Taken together, our approach evidenced that rabbit is one of the species displaying partial DNA demethylation in the PP, and for the first time demonstrated maintenance methylation activity in both pronuclei during the first S phase.

Reprogramming

Pronucleus

DNA demethylation

10.4161/epi.6.8.16073

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Analyses des conséquences du vieillissement sur la physiologie de l’endomètre à l’aide d’un modèle bovin de génome nucléaire fixé

HAL (Le Centre pour la Communication Scientifique Directe) (2018)

Olivier Sandra MarÃa E. LÃ pez Christophe Richard Pierrette Reinaud Audrey Lesage-Padilla

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Early development of bovine embryos is influenced by the origin of feeders during in vitro culture

HAL (Le Centre pour la Communication Scientifique Directe) (2015)

Anaïs Vitorino Carvalho Luc Jouneau Catherine Archilla Ludivine Laffont Sylvie Ruffini

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Implication de séquences rétrovirales endogènes dans l'établissement et le maintien de la pluripotence

HAL (Le Centre pour la Communication Scientifique Directe) (2010)

Alexei V. Evsikov Catherine Archilla Nathalie Peynot Nathalie Daniel K. Batiecoro

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Effets de l'exposition aux nanoparticules d'or par inhalation sur la fonction placentaire et le phénotype de la descendance

HAL (Le Centre pour la Communication Scientifique Directe) (2021)

Delphine Rousseau‐Ralliard Valérie Fessard Michèle Dahirel John Boere Christophe Richard

Avec pour thematique Ma mere, mon Placenta et Moi, ce colloque a pour objectif d’encourager la recherche sur le placenta et les pathologies obstetricales associees, de faciliter les collaborations internationales francophones, et les echanges scientifiques et methodologique dans notre domaine.

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