Nicolas Lafitte

Fluigent (France)

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Momoko Kumemura

Kyushu Institute of Technology

Laurent Jalabert

The University of Tokyo

Dominique Collard

The University of Tokyo

Hiroyuki Fujita

Laboratory for Integrated Micro-Mechatronic Systems

Yann Le Gorrec

Franche-Comté Électronique Mécanique Thermique et Optique - Sciences et Technologies

Yassine Haddab

Laboratoire d'Informatique, de Robotique et de Microélectronique de Montpellier

Christophe Yamahata

École Polytechnique Fédérale de Lausanne

Vladimir Gubala

Cambridge Consultants (United Kingdom)

V. J. Logeeswaran

University of California, Davis

Pablo Varona

Universidad Autónoma de Madrid

Cooperative Institutions

The University of Tokyo

Centre National de la Recherche Scientifique

Laboratory for Integrated Micro-Mechatronic Systems

Tokyo University of Science

Université de Lille

Université Claude Bernard Lyon 1

Chinese Academy of Sciences

Tokyo Institute of Technology

École Centrale de Lille

Institut d'électronique de microélectronique et de nanotechnologie

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ケイ素ナノピンセット(SNT)のチップの間でin situ生成されたDNAの直接電気的および機械的特性化

Lab on a Chip (2016)

L Karsten Stanislav Momoko Kumemura Jalabert Laurent Nicolas Lafitte C Kudo Lili

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Silicon Nanotweezers for Molecules and Cells Manipulation and Characterization

Advanced micro & nanosystems (2015)

Dominique Collard Nicolas Lafitte Hervé Guillou Momoko Kumemura Laurent Jalabert

In this chapter, Silicon Nanotweezer (SNT) is presented as a generic microdevice allowing bio-mechanical assays of filamentary molecules and cells. SNT operations and design are presented. The chapter explains the SNT microfabrication process; the operations and applications to molecules and cell characterization. Commonly integrated in microsystem designs, folded-beam springs are designed to minimize beams areas, decrease their mechanical stiffness, and enhance displacement ranges. In the current design, three sets of folded-beam suspensions support different parts of the system and provide the electrical connections for the actuation and sensing. In phenomenological models, the dynamic properties inform us on the stiffness and dissipation at the resonance frequency, whereas the static properties are related directly to the force. The monitoring of the resonance properties prevents any damage to the cell or to the SNT. To conclude, the silicon nanotweezers can be applied to handle various objects other than DNA molecules.

Microsystem

Characterization

10.1002/9783527690237.ch7

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Real-time mechanical characterization of DNA degradation under therapeutic X-rays and its theoretical modeling

Microsystems & Nanoengineering (2016)

Grégoire Perret T. Lacornerie Fabio Manca Stefano Giordano Momoko Kumemura

Abstract The killing of tumor cells by ionizing radiation beams in cancer radiotherapy is currently based on a rather empirical understanding of the basic mechanisms and effectiveness of DNA damage by radiation. By contrast, the mechanical behaviour of DNA encompassing sequence sensitivity and elastic transitions to plastic responses is much better understood. A novel approach is proposed here based on a micromechanical Silicon Nanotweezers device. This instrument allows the detailed biomechanical characterization of a DNA bundle exposed to an ionizing radiation beam delivered here by a therapeutic linear particle accelerator (LINAC). The micromechanical device endures the harsh environment of radiation beams and still retains molecular-level detection accuracy. In this study, the first real-time observation of DNA damage by ionizing radiation is demonstrated. The DNA bundle degradation is detected by the micromechanical device as a reduction of the bundle stiffness, and a theoretical model provides an interpretation of the results. These first real-time observations pave the way for both fundamental and clinical studies of DNA degradation mechanisms under ionizing radiation for improved tumor treatment.

radiation damage

Characterization

10.1038/micronano.2016.62

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Citations (25)

Dispersion

Springer eBooks (2012)

Biana Godin Elka Touítou Rajaram Krishnan Michael J. Heller Nicolas G. Green

10.1007/978-90-481-9751-4_100182

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Active control of silicon nanotweezers detects enzymatic reaction at the molecular level

Nicolas Lafitte Yassine Haddab Yann Le Gorrec H. Guillou Momoko Kumemura

This work has achieved the control of micromachined tweezers for the enhancement of the sensing of DNA molecules. The tool allows a routine trapping of the molecules and the real-time monitoring of the mechanical properties of the bundle of molecules. Monitoring the resonance response of the system, the bundle and the interactions of enzymes or proteins are characterized. Furthermore the design and the implementation of state feedback decrease the equivalent stiffness of the system and improve the sensitivity of the tool.

10.1109/transducers.2013.6627279

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DEVELOPMENT OF PROGRAMMABLE RESONATORS IN PZT-SOI TECHNOLOGY

「センサ・マイクロマシンと応用システム」シンポジウム論文集電気学会センサ・マイクロマシン部門 [編] (2013)

Nicolas Lafitte Takuya Takahashi Hiroyuki Fujita

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Dye Sensitized Solar Cells

Springer eBooks (2012)

Biana Godin Elka Touítou Rajaram Krishnan Michael J. Heller Nicolas G. Green

10.1007/978-90-481-9751-4_100196

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Silicon Nanotweezers with a microfluidic cavity for the real time characterization of DNA damage under therapeutic radiation beams

Grégoire Perret Po-Tsun Chiang T. Lacornerie Momoko Kumemura Nicolas Lafitte

We report the biomechanical characterization of λ-DNA bundle exposed to a therapeutic radiation beam by silicon Nanotweezers. The micromechanical device endures the harsh environment of radiation beams, and still retains molecular-level detection accuracy. The real-time DNA bundle degradation is observed in terms of biomechanical stiffness and viscosity reduction, both in air and in solution. These results pave the way for both fundamental and clinical studies of DNA degradation mechanisms under ionizing radiation for improved tumor treatment.

radiation damage

Characterization

Degradation

10.1109/embc.2013.6611123

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Citations (5)

DUV Photolithography and Materials

Springer eBooks (2012)

Biana Godin Elka Touítou Rajaram Krishnan Michael J. Heller Nicolas G. Green

10.1007/978-90-481-9751-4_370

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Citations (1)