A new species, Vibrio trachuri sp. nov., was isolated from the cultured Japanese horse mackerel (Trachurus japonicus). These Vibrio were Gram negative, motile rods and formed yellow colonies on BTB teepol and TCBS plate, turned TSI medium to yellow and was sensitive to 150 microM O/129 (2,4-diamino-6,7-diisopropyl pteridine phosphate) like Listonella anguillarum which has been described as Vibrio anguillarum. However, the results of VP test and decarboxylation of lysine or dihydrolation of arginine suggested that these Vibrio are rather closely related to V. parahaemolyticus. DNA similarity determined by the microplate hybridization technique revealed that these Vibrio are genetically quite distant from Listonella anguillarum or V. parahaemolyticus and rather close to V. harveyi, although there was no Vibrio species which had more than 70% similarity value. From these results we propose to nominate Vibrio trachuri sp. nov. for this new Vibrio species.
The aim of this study was to determine whether chicken sperm express avian beta-defensin 3 (AvBD-3), an antimicrobial peptide, and retain it during the residence in the male and female reproductive tract. Immunocytochemistry to identify AvBD-3 was performed in the testis, epididymis, ductus deferens, ejaculated sperm, and utero-vaginal junction of the oviduct of inseminated hens. Reverse transcription-PCR analysis was also performed to confirm the synthesis of AvBD-3 by testicular cells and ejaculated sperm. The immunoreaction products for AvBD-3 were identified in the elongated spermatids attached to the seminiferous epithelium and in the sperm in the lumen of seminiferous tubules. Immunoreaction products were found in the sperm in the epididymal ducts and ductus deferens. In the ejaculated sperm, immunoreaction products were distributed in the midpiece and cephalic region of the tail of sperm, but not in the head. The AvBD-3 immunoreaction products were also identified in the sperm stored in the sperm storage tubules in the utero-vaginal junction. Reverse transcription-PCR analysis showed the expression of AvBD-3 mRNA in the testicular tissue, but not in the ejaculated sperm. These results suggest that AvBD-3 is synthesized by late stage of spermatids in the testis, and this molecule is retained by the sperm during the passage of male reproductive tract, and even in the sperm storage tubules of oviduct. The sperm AvBD-3 is assumed to play roles in protecting the sperm from the microbial infection and enable to survive in the male and female reproductive tracts.
The familial occurrence of the chromosome aberrations has been a rare event.In the present paper, a family with the XY-female and two 3/4 reciprocal translocations is to be described.Case reports.The propositus was a 5-year-old girl of the heavy mental retardation, 16 kg in weight, 10 cm in length.She was born to a 28-year-old mother and a 31-year-old father as the second child in the second pregnancy.There was no history of the abortion, still birth, congenital malformations, the exposure to the atomic bomb, and consanguinity in this couple.The clinical examination revealed that her parents and elder sister were phenotypically normal.The propositus was born prematurely, with severe cyanosis.The birth weight of the patient was 3150 g.The remarkable clinical signs of this propositus were flat occiput, peromelie and bowing of the forearm at right, radius aplasia, idiot.Cytological findings.The chromosome preparations were made on the leucocyte cultures from the propositus, her parents and her elder sister.The G-and Q-banding differential stainings were applied for the chromosome identification.The chromosome counts were made with 65-well delineated metaphases.The karyotype was analysed in 15 cells by the conventional Giemsa specimens, 24 cells by G-banding, and 26 cells by Q-banding differential staining.
