Shiwani Guleria

Lovely Professional University

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Trends

Author Order

Document Type

Co-Authors

Abhishek Walia

Chaudhary Sarwan Kumar Himachal Pradesh Krishi Vishvavidyalaya

Anjali Chauhan

Thapar Institute of Engineering & Technology

C. K. Shirkot

Dr. Yashwant Singh Parmar University of Horticulture and Forestry

Preeti Mehta

Indian Oil Corporation (India)

Rishi Mahajan

Jaypee University of Information Technology

Vineet Kumar

Lovely Professional University

Praveen Guleria

DAV University

Saurabh Kulshrestha

Shoolini University

Samriti Sharma

Chandigarh University

Anil Kumar

Sido Kanhu Murmu University

Cooperative Institutions

Dr. Yashwant Singh Parmar University of Horticulture and Forestry

Chaudhary Sarwan Kumar Himachal Pradesh Krishi Vishvavidyalaya

Shoolini University

Institute of Himalayan Bioresource Technology

Chaudhary Charan Singh Haryana Agricultural University

Jaypee University of Information Technology

Central University of Himachal Pradesh

Chandigarh University

Lovely Professional University

DAV University

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Field

Isolation and Partial Characterization of Surface Producing Bacterial Strain Producing Amylase from Soil

International Journal of Bio-resource and Stress Management (2018)

Samriti Sharma Shiwani Guleria Arvind Behl Navneet Batra

Starch is an important constituent of the human diet and is a major storage product of many economically important crops such as wheat, rice, maize, tapioca, and potato.The production of α-amylase is essential for conversion of starches into oligosaccharides.Amylase is one of the most important enzymes used in industry such as in textile, food, paper and pharmaceutical industry etc. Amylase has ability to hydrolyzed starch molecules into polymer composed of glucose unit.The aim of present study was to isolate amylase producing bacteria from soil samples, screening of amylase producing strains, morphological and biochemical characterizations of strains, and optimized cultural conditions of the strains.The soil samples were collected from deep soil of campus at Chandigarh with the help of aseptic spectula.The bacterial strains were isolated by serial dilution and plating method.Three bacterial strains such as RT03, RT5 and T5 having amylolytic activity was isolated.Based on intensity of clear zone one strain were selected for further study.The morphological characterization of strain RT5 showed that it is rod shaped bacilli, cream color, raised elevation, entire margin, moderate size and Gram negative in nature.Enzymatic activity of strain RT5 was estimated at various pH (6, 7 and 8) and temperatures (30 °C, 37 °C, 50 °C and 60 °C).The best amylolytic activity of RT5 was observed at pH 6 with optimum temperature 50 °C.2006).Due to the increasing demand for these enzymes in various industries, there is enormous interest in developing enzymes with better properties such as raw starch degrading amylases suitable for industrial applications and their cost effective production techniques.

Strain (injury)

10.23910/ijbsm/2018.9.1.3c0956

Cite

Citations (1)

Genotypic and Phenotypic Profile of Alkalophile Proteolytic Bacillus sp. Associated with Rhizosphere of Apple Trees in Trans Himalayas

Proceedings of the National Academy of Sciences India Section B Biological Sciences (2014)

Shiwani Guleria Abhishek Walia Anjali Chauhan C. K. Shirkot

Bacillus amyloliquefaciens

10.1007/s40011-014-0447-z

Cite

Citations (6)

Toxic Effects of Nanomaterials to Plants and Beneficial Soil Bacteria

CRC Press eBooks (2018)

Shiwani Guleria Praveen Guleria Vineet Kumar

Nanoparticles and their synthesis have increased recently because of their excessive use in different commercial products. This is one of the reasons of exposure of nanoparticles to beneficial microflora and plants over the past few decades. Soil microorganisms play a very important role in maintaining soil health, ecosystem functions, and crop productivity. Therefore, it is very important to evaluate overall positive or negative effect of nanoparticles on soil microorganisms. Environmentally friendly synthesis of nanoparticle by microbes and plants is an alternative method. However, the question still remains the same, i.e., what will be the effect of biological nanoparticles on living flora? Therefore, this chapter discusses the impact of nanoparticles on plants and microbes along with its potential applications.

Nanomaterials

10.1201/9781351252966-8

Cite

Citations (0)

Purification and characterization of detergent stable alkaline protease from Bacillus amyloliquefaciens SP1 isolated from apple rhizosphere

Journal of Basic Microbiology (2015)

Shiwani Guleria Abhishek Walia Anjali Chauhan C. K. Shirkot

A thermostable extracellular alkaline protease producing Bacillus amyloliquefaciens SP1 was isolated from apple rhizosphere having multifarious plant growth promoting activities. Strain SP1 was purified to 6.48-fold using four-step purification protocol and characterized in detail for its robustness and ecofriendly application in leather and detergent industries. Structural analysis revealed that the protease was monomeric and had a molecular weight of 43 kDa. It exhibited optimum activity at 60°C in alkaline environment (pH 8.0) and stable in the presence of surfactants and oxidizing agents. Enzyme was thermostable at 50°C and retained more than 70% activity after 30 min incubation. It has shown stain removal property and dehairing of goat skin without chemical assistance and hydrolyzing fibrous proteins. This protease showed Km of 0.125 mg ml(-1) and V(max) of 12820 μg ml(-1) indicating its excellent affinity and catalytic role. Thermal inactivation of the pure enzyme followed first-order kinetics. The half life of the pure enzyme at 50, 60, and 65°C was 77, 19.80, and 13.33 min, respectively. The activation energy was 37.19 KJ mol(-1). The results suggest that the B. amyloliquefaciens SP1 has a potential application in different industries.

Bacillus amyloliquefaciens

Enzyme Kinetics

10.1002/jobm.201500341

Cite

Citations (46)

Improvement for enhanced xylanase production by Cellulosimicrobium cellulans CKMX1 using central composite design of response surface methodology

3 Biotech (2015)

Abhishek Walia Preeti Mehta Shiwani Guleria C. K. Shirkot

The effects of yeast extract (X1), NH4NO3 (X2), peptone (X3), urea (X4), CMC (X5), Tween 20 (X6), MgSO4 (X7), and CaCO3 (X8) on production of xylanase from Cellulosimicrobium cellulans CKMX1 were optimized by statistical analysis using response surface methodology (RSM). The RSM was used to optimize xylanase production by implementing the Central composite design. Statistical analysis of the results showed that the linear, interaction and quadric terms of these variables had significant effects. However, only the linear effect of X4, X5, interaction effect of X1X7, X1X8, X2X3, X2X8, X3X6, X3X8, X4X6, X4X7, X5X7, X5X8 and quadratic effect of X 32 , X 52 and X 72 found to be insignificant terms in the quadratic model and had no response at significant level. The minimum and maximum xylanase production obtained was 331.50 U/g DBP and 1027.65 U/g DBP, respectively. The highest xylanase activity was obtained from Run No. 30, which consisted of yeast extract (X1), 1.00 g (%); NH4NO3 (X2), 0.20 g (%); peptone (X3), 1.00 g (%); urea (X4), 10 mg (%); CMC (X5), 1.00 g (%); Tween 20 (X6), 0.02 mL (%); CaCO3 (X7), 0.50 g (%) and MgSO4 (X8), 9.0 g (%). The optimization resulted in 3.1-fold increase of xylanase production, compared with the lowest xylanase production of 331.50 U/g DBP after 72 h of incubation in stationary flask experiment. Application of cellulase-free xylanase in pulp biobleaching from C. cellulans CKMX1 under C-EP-D sequence has been shown to bring about a 12.5 % reduction of chlorine, decrease of 0.8 kappa points (40 %), and gain in brightness was 1.42 % ISO points in 0.5 % enzyme treated pulp as compared to control.

Central composite design

10.1007/s13205-015-0309-2

Cite

Citations (30)

Molecular characterization of alkaline protease of Bacillus amyloliquefaciens SP1 involved in biocontrol of Fusarium oxysporum

International Journal of Food Microbiology (2016)

Shiwani Guleria Abhishek Walia Anjali Chauhan C. K. Shirkot

Bacillus amyloliquefaciens

10.1016/j.ijfoodmicro.2016.05.030

Cite

Citations (58)

Immobilization of Bacillus amyloliquefaciens SP1 and its alkaline protease in various matrices for effective hydrolysis of casein

3 Biotech (2016)

Shiwani Guleria Abhishek Walia Anjali Chauhan C. K. Shirkot

An extracellular alkaline protease producing B. amyloliquefaciens SP1 was isolated from apple rhizosphere having multifarious plant growth-promoting activities. B. amyloliquefaciens SP1 protease was immobilized using various concentrations of calcium alginate, agar and polyacrylamide to determine the optimum concentration for formation of the beads. Enzyme activity before immobilization (at 60 °C, pH 8.0 for 5 min) was 3580 µg/ml/min. The results of immobilization with various matrices revealed that 3 % calcium alginate (2829.92 µg/ml/min), 2 % agar (2600 µg/ml/min) and 10 % polyacrylamide (5698.99 µg/ml/min) were optimum concentrations for stable bead formation. Immobilized enzyme reusability results indicated that calcium alginate, agar and polyacrylamide beads retained 25.63, 22.05 and 34.04 % activity in their fifth repeated cycle, respectively. In cell immobilization technique, the free movement of microorganisms is restricted in the process, and a semi-continuous system of fermentation can be used. In the present work, this technique has been used for alkaline protease production using different matrices. Polyacrylamide (10 %) was found with the highest total alkaline protease titer, i.e., 24,847 µg/ml/min semi-continuously for 18 days as compared to agar (total enzyme titer: 5800 in 10 days) and calcium alginate (total enzyme titer: 13,010 in 15 days). This present study reported that polyacrylamide (10 %) among different matrices has maximum potential of immobilization of B. amyloliquefaciens SP1 and its detergent stable alkaline protease with effective application in bloodstain removal.

Calcium alginate

Polyacrylamide

Bacillus amyloliquefaciens

10.1007/s13205-016-0519-2

Cite

Citations (19)

Optimization of milk-clotting enzyme production by Bacillus amyloliquefaciens SP1 isolated from apple rhizosphere

Bioresources and Bioprocessing (2016)

Shiwani Guleria Abhishek Walia Anjali Chauhan C. K. Shirkot

Present study aims to isolate and optimize fermentation conditions of milk-clotting enzyme producing rhizobacteria Bacillus amyloliquefaciens SP1. A bacterium producing an extracellular milk-clotting enzyme (MCE) was isolated from the rhizosphere of the planted population of apple trees growing at Distt. Chamba of Himachal Pradesh, India. According to morphological, physiological, biochemical, and molecular characterization, isolate was identified as B. amyloliquefaciens. Single-factor testing was used to study the optimum physical conditions and nutritional parameters for production of the MCE per proteolysis activity which insures its usefulness as new source of milk coagulant for cheese making. The optimum conditions for production of the milk-clotting enzyme were: temperature, 30 °C; inoculum size, 1 %; and initial pH of the medium, 6.0. The maximum milk-clotting activity and milk-clotting activity per proteolysis activity were found using soyabean as nitrogen source and sucrose as carbon source. These optimized conditions resulted in a 1.9-fold increase in production of the milk-clotting enzyme. This study reported a plant growth promoting rhizobacteria, i.e., B. amyloliquefaciens as source of milk-clotting enzyme which has potential as a calf rennet substitute.

Bacillus amyloliquefaciens

Rennet

Clotting time

10.1186/s40643-016-0108-6

Cite

Citations (14)

Isolation and Characterization of Bacillus sp. with Their Effect on Growth of Tomato Seedlings

Indian Journal of Agricultural Biochemistry (2014)

Anjali Chauhan Shiwani Guleria Abhishek Walia Rishi Mahajan Seema Verma

The use of plant growth-promoting rhizobacteria (PGPR) is steadily increasing in agriculture and offers an attractive way to replace chemical fertilizers, pesticides, and supplements. In search of efficient PGPR with multifarious traits, thirty bacterial strains were isolated from tomato rhizosphere of District Solan, Himachal Pradesh using modified replica plating technique. The isolates were screened in vitro for their plant growth promoting (PGP) activities. Five isolates, with a score greater than sixty per cent, exhibiting multiple activities, were evaluated quantitatively for PGP traits. All the five isolates with multifarious PGP activities were selected for net house studies. Among them, three isolates i.e. CKT1, CKT4 and CKT5 showed growth promotional effects and other two isolates i.e CKT2 and CKT3 were found to have detrimental effects on plant growth. Phylogenetic analysis has confirmed that five isolates though showing similarity to Bacillus subtilis yet were clustered as two different groups, thus highlighting their strain level differences. Multiple regression analysis, carried out to estimate the overall impact of PGP activities (Phosphate solubilization, Indole acidic acid and siderophore production) on morphological characters viz. root length and dry weight, shoot length and dry weight was resulted in highly significant r2 values.

Phosphate solubilizing bacteria

Non-invasive ventilation

Dry weight

Source

Cite

Citations (5)

Impact of Fungicide Mancozeb at Different Application Rates on Soil Microbial Populations, Soil Biological Processes, and Enzyme Activities in Soil

The Scientific World JOURNAL (2014)

Abhishek Walia Preeti Mehta Shiwani Guleria Anjali Chauhan C. K. Shirkot

The use of fungicides is the continuous exercise particularly in orchard crops where fungal diseases, such as white root rot, have the potential to destroy horticultural crops rendering them unsaleable. In view of above problem, the present study examines the effect of different concentrations of mancozeb (0–2000 ppm) at different incubation periods for their harmful side effects on various microbiological processes, soil microflora, and soil enzymes in alluvial soil (pH 6.8) collected from apple orchards of Shimla in Himachal Pradesh (India). Low concentrations of mancozeb were found to be deleterious towards fungal and actinomycetes population while higher concentrations (1000 and 2000 ppm) were found to be detrimental to soil bacteria. Mancozeb impaired the process of ammonification and nitrification. Similar results were observed for nitrifying and ammonifying bacteria. Phosphorus solubilization was increased by higher concentration of mancozeb, that is, 250 ppm and above. In unamended soil, microbial biomass carbon and carbon mineralization were adversely affected by mancozeb. Soil enzymes, that is, amylase, invertase, and phosphatase showed adverse and disruptive effect when mancozeb used was above 10 ppm in unamended soil. These results conclude that, to lessen the harmful effects in soil biological processes caused by this fungicide, addition of higher amount of nitrogen based fertilizers is required.

10.1155/2014/702909

Cite

Citations (54)