The effect of increasing doses of estradiol, 50 mcg-1 mg, or testosterone propionate, 500 mcg-10 mg, given to a pregnant rat on pregnancy Day 14-21 on development of the preputial gland in male and female fetuses was compared to the effect on anogenital distance and retardation of weight gain. Estradiol in increasing doses stimulated development of prepuital gland in both sexes, increased dispersion of data in males, and high doses stimulated gland development in females almost as much as in males. Estradiol at 50 mcg to 1 mg decreased anogenital distance in males from 3.2 to 1.6 mm, and increased it in females from 1.1 to 1.7 mm. Testosterone propionate stimulated preputial gland development in females, and increased weight even more so; it inhibited gland development slightly in males. Testosterone at 500 mcg-10 mg augmented anogenital distance in females, but had no effect on males except at 10 mg.
