Jianxin Lü

Wenzhou Medical University

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Min‐Xin Guan

Children's Hospital of Zhejiang University

Yidong Bai

The University of Texas Health Science Center at San Antonio

Yi Zhu

Suzhou Municipal Hospital

Li Yang

Qilu Hospital of Shandong University

Xiaowen Tang

Soochow University

Guoqiang Tan

Wenzhou Medical University

Huangen Ding

Louisiana State University

Aifen Yang

Hangzhou Normal University

Jindan Wang

Wenzhou Medical University

Hezhi Fang

Wenzhou Medical University

Cooperative Institutions

Wenzhou Medical University

196

Chinese Academy of Sciences

Zhejiang University

Sun Yat-sen University

Shanghai Jiao Tong University

Nanjing Medical University

Huazhong University of Science and Technology

Chinese Academy of Medical Sciences & Peking Union Medical College

Fudan University

Sichuan University

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Sargassum fusiforme polysaccharide activates nuclear factor kappa-B (NF-κB) and induces cytokine production via Toll-like receptors

Carbohydrate Polymers (2014)

Xiaohong Chen Guoqing Yu Sairong Fan Manman Bian Huijun Ma

Pyrrolidine dithiocarbamate

Proinflammatory cytokine

10.1016/j.carbpol.2014.01.056

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Citations (48)

A novel immunotherapy for superficial bladder cancer by intravesical immobilization of GM‐CSF

Journal of Cellular and Molecular Medicine (2009)

Zhiming Hu Wanlong Tan Lin Zhang Zhongkun Liang Cuixiang Xu

Abstract In situ gene therapy with granulocyte‐macrophage colony‐stimulating factor (GM‐CSF) was demonstrated to successfully inhibit tumour cell growth in a mouse orthotopic bladder cancer model, but suffered from several disadvantages, such as limited efficiency for gene delivery, low expression efficiency of the transgene and the safety concern resulting from viral vector. In order to address the limits, a novel immunotherapy was developed attentively through immobilization of streptavidin‐tagged bioactive GM‐CSF on the biotinylated mucosal surface of bladder wall on the basis of both the unique property of streptavidin (SA) to bind rapidly and almost irreversibly to any biotin‐linked molecule and the outstanding ability of biotin to be incorporated easily into the proteins on the cell surface. The mouse orthotopic model of MB49 bladder cancer was used to evaluate the feasibility and efficacy of the novel immunotherapy performed twice a week for 3 weeks. Briefly, 1 day after intravesical implantation of 1 × 10 6 MB49 tumour cells in C57BL/6 mouse, 100 μl of 1 mg/ml NHS‐PEO4‐biotin was instilled and allowed to incubate in the bladder for 30 min., followed by intravesical instillation of 100 μl of 0.15 mg/ml SA‐GM‐CSF bifunctional fusion protein and incubation for 1 hr. SA‐GM‐CSF fusion protein was shown to be immobilized efficiently and durably on the biotinylated mucosal surface of bladder wall. The bladder cancer incidence was dramatically decreased from 100% in the control group to 37.5% in the SA‐GM‐CSF group. Importantly, 70% of the SA‐GM‐CSF‐cured mice were protected against a second intravesical wild‐type MB49 tumour challenge, indicating that an effective anti‐tumour immunity was generated against MB49 bladder cancer. Thus, the novel immunotherapy may be an attractive therapeutic alternative and should be evaluated in bladder cancer patients.

Streptavidin

Cancer Immunotherapy

10.1111/j.1582-4934.2009.00818.x

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Citations (20)

Spectrum and frequency of mitochondrial 12S rRNA variants in the Chinese subjects with nonsynrdomic hearing loss in Zhejiang Prov-ince

Hereditas (Beijing) (2012)

Binjiao Zheng Guang-Hua Peng Bobei Chen Fang Fang Jing Zheng

Mitochondrial DNA (mtDNA) mutations are one of the important causes of deafness. In particular, the 12S rRNA gene is the hot spots for mutations associated with both aminoglycoside ototoxicity and nonsyndromic deafness. In this report, a total of 318 Chinese pediatric hearing-impaired subjects were recruited from otology clinics in the Zhejiang Province, China. These subjects underwent clinical, genetic evaluation and molecular analysis of 12S rRNA gene. Mutational analysis identified 34 variants in the 12S rRNA gene in this cohort. The incidences of the known deafness-associated 1555A>G, 1494C>T and 1095T>C mutations were 9.1%, 0.6% and 1.25% in this cohort, respectively. Other mtDNA variants were evaluated by structural and phylogenetic analysis. Of these, the 839A>G and 1452T>C variants could confer increased sensitivity to aminoglycosides or nonsyndromic deafness as they were not present in 449 Chinese controls and localized at highly conserved nucleotides of the 12S rRNA. However, other variants appeared to be polymorphisms. These data further support the idea that mitochondrial 12S rRNA is one of major targets for aminoglycoside ototoxicity. These data have been providing valuable information to predict which individuals are at risk for ototoxicity, to improve the safety of aminoglycoside antibiotic therapy, and eventually to decrease the incidence of deafness.

Han Chinese

Ototoxicity

Genetic Analysis

10.3724/sp.j.1005.2012.00695

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Citations (3)

Protective effect of the polysaccharide from Ophiopogon japonicus on streptozotocin-induced diabetic rats

Carbohydrate Polymers (2013)

Xiaohong Chen Jia Tang Wenyuan Xie Junjun Wang Jingling Jin

Intraperitoneal injection

10.1016/j.carbpol.2013.01.037

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Citations (71)

The tRNAGly T10003C mutation in mitochondrial haplogroup M11b in a Chinese family with diabetes decreases the steady-state level of tRNAGly, increases aberrant reactive oxygen species production, and reduces mitochondrial membrane potential

Molecular and Cellular Biochemistry (2015)

Wei Li Chaowei Wen Weixing Li Hailing Wang Xiaomin Guan

Haplogroup

10.1007/s11010-015-2493-0

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Citations (13)

Low expression of Foxo3a is Associated with Poor Prognosis in Ovarian Cancer Patients

Cancer Investigation (2009)

Min Fei Yueming Zhao Yuchan Wang Mudan Lu Chun Cheng

To investigate whether Foxo3a expression is correlated with p27kip1 protein levels as well as how it might be clinically relevant, we evaluated the expression of Foxo3a in several ovarian tumors. Immunohistochemical analysis was performed in 63 cases of ovarian tumors. Ten cases were evaluated by Western blot analysis. There was a correlation observed between Foxo3a over-expression and clinic pathological parameters (p = 0.032). Kaplan-Meier survival analysis showed that Foxo3a low expression was significantly associated with poor prognosis of patients. It may be a useful prognostic marker and target in ovarian cancer.

10.1080/07357900802146204

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Citations (78)

The polymorphism in the let-7 targeted region of the Lin28 gene is associated with increased risk of type 2 diabetes mellitus

Molecular and Cellular Endocrinology (2013)

Jie Zhang Longyi Zhang Runping Fan Nengneng Guo Chunhui Xiong

Pathogenesis

10.1016/j.mce.2013.04.022

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Citations (19)

Mitochondrial haplotypes may modulate the phenotypic manifestation of the deafness-associated 12S rRNA 1555A>G mutation

Mitochondrion (2009)

Jianxin Lü Yaping Qian Zhiyuan Li Aifen Yang Yi Zhu

Haplogroup

Penetrance

Expressivity

10.1016/j.mito.2009.09.007

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Citations (102)

Exposure to high glutamate concentration activates aerobic glycolysis but inhibits ATP‐linked respiration in cultured cortical astrocytes

Cell Biochemistry and Function (2014)

Yao Shen Yueyang Tian Xiaojie Shi Jianbo Yang Ouyang Li

Astrocytes play a key role in removing the synaptically released glutamate from the extracellular space and maintaining the glutamate below neurotoxic level in the brain. However, high concentration of glutamate leads to toxicity in astrocytes, and the underlying mechanisms are unclear. The purpose of this study was to investigate whether energy metabolism disorder, especially impairment of mitochondrial respiration, is involved in the glutamate-induced gliotoxicity. Exposure to 10-mM glutamate for 48 h stimulated glycolysis and respiration in astrocytes. However, the increased oxygen consumption was used for proton leak and non-mitochondrial respiration, but not for oxidative phosphorylation and ATP generation. When the exposure time extended to 72 h, glycolysis was still activated for ATP generation, but the mitochondrial ATP-linked respiration of astrocytes was reduced. The glutamate-induced astrocyte damage can be mimicked by the non-metabolized substrate d-aspartate but reversed by the non-selective glutamate transporter inhibitor TBOA. In addition, the glutamate toxicity can be partially reversed by vitamin E. These findings demonstrate that changes of bioenergetic profile occur in cultured cortical astrocytes exposed to high concentration of glutamate and highlight the role of mitochondria respiration in glutamate-induced gliotoxicity in cortical astrocytes.

Bioenergetics

Cellular respiration

Glutamate aspartate transporter

Adenosine triphosphate

Neurotoxicity

10.1002/cbf.3047

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Citations (11)

Mitochondrial tRNAGlu A14693G variant may modulate the phenotypic manifestation of deafness-associated 12S rRNA A1555G mutation in a Han Chinese family

Journal of genetics and genomics/Journal of Genetics and Genomics (2009)

Yu Ding Yongyan Li Junyan You Li Yang Bobei Chen

Penetrance

Expressivity

Haplogroup

10.1016/s1673-8527(08)60111-3

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Citations (26)