Guoqiang Zhu

Yangzhou University

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Pengpeng Xia

Yangzhou University

Qiangde Duan

Yangzhou University

Mingxu Zhou

Jiangsu Academy of Agricultural Sciences

Philip R. Hardwidge

Kansas State University

Xia Meng

Yangzhou University

Chunhong Zhu

Air Force Medical University

Yaoyao Xia

Shandong Agricultural University

Yi‐Ting Wang

Chinese Academy of Sciences

Wenkai Ren

South China Agricultural University

Jianye Wang

Yangzhou University

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Yangzhou University

165

Chinese Academy of Sciences

Shanghai Jiao Tong University

Chinese Academy of Agricultural Sciences

Qingdao University

Ministry of Agriculture and Rural Affairs

Ministry of Education of the People's Republic of China

Chinese Academy of Medical Sciences & Peking Union Medical College

University of Chinese Academy of Sciences

Peking University

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High-Sensitivity/Specificity Colorimetric Determination of Viable Staphylococcus Aureus in Ready-to-Eat Foods Using Mimicking Peroxidase and Temperate Bacteriophage

Wenyuan Zhou Yajie Li Guoqiang Zhu Zhenquan Yang Xuewen Gu

Herein, four temperate Staphylococcus aureus phages were isolated, and their biological features (one-step growth, host range, pH stability, temperature stability, and adsorption rate) were evaluated as the biological element of bacteriophage-based biosensors. The selected phage SapYZUs7 was immobilized on the MnCo2O4 nanozyme to prepare SapYZUs7@MnCo2O4, and its application in ready-to-eat foods for S. aureus detection was evaluated. Compared to the other three S. aureus phages, SapYZUs7 exhibit a higher titer, broader host range, stronger pH stability (3–12), outstanding absorption rate (71.6%, 6 min), and unique gene content suitable for the S. aureus detection. The characterization of SapYZUs7@MnCo2O4 revealed its successful immobilization, excellent biological activity, and peroxidase-mimicking ability. Moreover, the SapYZUs7@MnCo2O4 exhibited an outstanding sensitivity and specificity for the rapid colorimetric determination of viable S. aureus counts with a limiting detection of 1.14 × 102 CFU mL-1. In addition, SapYZUs7@MnCo2O4 was successfully employed for the colorimetric determination of S. aureus in food samples while ignoring the different food preservatives, NaCl concentration, and pH values. In conclusion, the selection of phages based on biological and genetic characteristics are important to design a biosensor platform, and the SapYZUs7@MnCo2O4 is a powerful tool for the detection of viable S. aureus in food matrix.

Temperateness

10.2139/ssrn.4501987

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EP11.01-08 GSDME-dependent Pyroptosis Affects the Prognosis and Response to Immunotherapy in Lung Adenocarcinoma

Journal of Thoracic Oncology (2023)

Pengfei Cao Guoqiang Zhu Ye Li Hua Huang Wu Dong

Pyroptosis

10.1016/j.jtho.2023.09.1158

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Betaine Inhibits Interleukin-1β Production and Release: Potential Mechanisms

Frontiers in Immunology (2018)

Yaoyao Xia Shuai Chen Guoqiang Zhu Ruilin Huang Yulong Yin

Betaine is a critical nutrient for mammal health, and has been found to alleviate inflammation by lowering interleukin (IL)-1β secretion; however, the underlying mechanisms by which betaine inhibits IL-1β secretion remain to be uncovered. In this review, we summarize the current understanding about the mechanisms of betaine in IL-1β production and release. For IL-1β production, betaine affects canonical and non-canonical inflammasome-mediated processing of IL-1β through signaling pathways, such as NF-κB, NLRP3 and caspase-8/11. For IL-1β release, betaine inhibits IL-1β release through blocking the exocytosis of IL-1β-containing secretory lysosomes, reducing the shedding of IL-1β-containing plasma membrane microvesicles, suppressing the exocytosis of IL-1β-containing exosomes, and attenuating the passive efflux of IL-1β across hyperpermeable plasma membrane during pyroptotic cell death, which are associated with ERK1/2/PLA2 and caspase-8/A-SMase signaling pathways. Collectively, this review highlights the anti-inflammatory property of betaine by inhibiting the production and release of IL-1β, and indicates the potential application of betaine supplementation as an adjuvant therapy in various inflammatory diseases associating with IL-1β secretion.

Betaine

10.3389/fimmu.2018.02670

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Attenuated porcine-derived type 2 bovine viral diarrhea virus as vector stably expressing viral gene

Journal of Virological Methods (2020)

Jie Tao Benqiang Li Ying Shi Jinghua Chen Guoqiang Zhu

clone (Java method)

Vero cell

10.1016/j.jviromet.2020.113842

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Effects of the -791(C→T) mutation in the promoter for tumor necrosis factor alpha on gene expression and resistance of Large White pigs to enterotoxigenic Escherichia coli F18.

PubMed (2016)

Ying Liu Chaohui Dai Li Sun Guoqiang Zhu Shenglong Wu

Tumor necrosis factor alpha (TNF-α) plays an important role in the immune system. In this study, TNF-α expression was analyzed in 11 tissues of 8 piglets resistant to enterotoxigenic Escherichia coli (ETEC) F18 and 8 ETEC F18-susceptible piglets from the Large White breed. The expression levels of TNF-α were high in immune organs (spleen, lung, thymus, and lymph nodes). The levels were higher in ETEC F18-resistant piglets than in ETEC F18-susceptible piglets, with significant differences in spleen, kidney, thymus, lymph node, and duodenum (P < 0.05). The mutation TNF-α -791(C→T) and 3 genotypes (CC, CT, and TT) were identified. The TNF-α expression levels in the spleen, kidney, lymph nodes, and duodenum were significantly higher in the TT pigs than in the CC pigs (P < 0.05). Thus, TNF-α -791(C→T) has significant effects on mRNA expression and may regulate ETEC F18 resistance of weaning piglets. Therefore, the -791(C→T) mutation of the TNF-α gene could be considered an important potential genetic marker of ETEC F18 resistance.

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The molecular adjuvant mC3d enhances the immunogenicity of FimA from type I fimbriae of Salmonella enterica serovar Enteritidis

Journal of Microbiology Immunology and Infection (2013)

Hassan-Hussein Musa Weijuan Zhang Jing Lv Xiaoli Duan Yang Yang

Polyclonal antibodies

Salmonella enteritidis

Salmonella enterica

10.1016/j.jmii.2012.11.004

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Citations (13)

Cytokine gene expression of peripheral blood lymphocytes stimulated by lipopolysaccharide

AFRICAN JOURNAL OF BIOTECHNOLOGY (2009)

Hassan Hussein Musa Yunhua Xu Baoying Dai Xiaoyan Kang Chunhong Zhu

Lipopolysaccharide (LPS) is a predominant glycolipid in the outer membranes of gam-negative bacteria that stimulates monocytes, macrophages, and neutrophils to produce cytokines. The aim was to study the expression profile of TLRs and cytokines and determine the role of LPS in the peripheral blood lymphocytes. Lymphocytes were isolated from the peripheral blood of chickens and induced with LPS, total RNA was extracted and reverse transcribed into cDNA. The mRNA expression level of TLRs and inflammatory cytokines were quantitatively determined by semi-quantitative RT-PCR. The result showed that lymphocytes were expressed mRNA of TLRs (TLR1/6/10, TLR3, TLR4, TLR5 and TLR7) and interleukins (IL-1�, IL-8, IL-18 and TGF-�4). LPS will significantly (P 0.05) induce the mRNA expression of TLR1/6/10, TLR5 and TGF-�4. LPS plays an important role in the innate immune response of lymphocytes to pathogen.

TLR5

TLR3

TLR7

10.5897/ajb2009.000-9163

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Transcriptomic analysis of granulosa cell populations proximal and distal to the germinal disc of chicken preovulatory follicles

Scientific Reports (2021)

Guoqiang Zhu Chao Fang Chunheng Mo Yajun Wang Yan Huang

Abstract Within the oocytes of chicken preovulatory follicles, the engulfed yolk constitutes 99% of the oocyte content, while the small germinal disc (GD) (which contains the nucleus and 99% ooplasm) occupies only less than 1%. Relative to the position of the GD, the single granulosa cell layer surrounding the oocyte can be sub-divided into two sub-populations: granulosa cells proximal (named Gp cells) and distal (Gd cells) to the GD. It was reported that Gp cells and Gd cells differ in their morphology, proliferative rate and steroidogenic capacity, however, the underlying mechanism controlling granulosa cell heterogeneity remains unclear. Here we analyzed the transcriptomes of Gd and Gp cells of preovulatory (F5 and F1) follicles in chicken ovaries. We found that: (1) genes associated with cell cycle and DNA replication ( CDK1 , CCNB3 etc.) have comparatively higher expression levels in Gp cells than in Gd cells, while genes associated with steroidogenesis ( CYP51A1 , DHCR24 ) are highly expressed in Gd cells, indicating that Gp cells are likely more mitotic and less steroidogenic than Gd cells; (2) genes associated with extracellular matrix remodeling, cell adhesion and sperm binding (ZP3, ZP2) are differentially expressed in Gp and Gd cells; (3) Furthermore, signaling molecules (WNT4/IHH) and receptors for NGF (NGFR), epidermal growth factor (EGFR), gonadotropins (FSHR/LHR) and prostaglandin (PTGER3) are abundantly but differentially expressed in Gp and Gd cells. Taken together, our data strongly supports the notion that Gp and Gd cells of preovulatory follicles differ in their proliferation rate, steroidogenic activity, ECM organization and sperm binding capacity, which are likely controlled by gonadotropins and local ovarian factors, such as GD-derived factors.

Granulosa cell

10.1038/s41598-021-84140-w

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Citations (18)

Effective treatment of a broad-host-range lytic phage SapYZU15 in eliminating Staphylococcus aureus from subcutaneous infection

Microbiological Research (2023)

Hua Wen Wenyuan Zhou Ying Wu Yajie Li Guoqiang Zhu

Lytic cycle

Subcutaneous abscess

10.1016/j.micres.2023.127484

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Citations (3)

Cell membrane-anchored MUC4 promotes tumorigenicity in epithelial carcinomas

Oncotarget (2016)

Pengpeng Xia Agnes Hakyung Choi Zengping Deng Yuqian Yang Jing Zhao

// Pengpeng Xia 1,2 , Agnes Hakyung Choi 1,2 , Zengping Deng 3 , Yuqian Yang 1,2 , Jing Zhao 1,2 , Yiting Wang 1,2 , Philip R. Hardwidge 4 and Guoqiang Zhu 1,2 1 College of Veterinary Medicine, Yangzhou University, Yangzhou, China 2 Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China 3 Nanjing Hospital of T.C.M, Nanjing, China 4 College of Veterinary Medicine, Kansas State University, Manhattan, KS, USA Correspondence to: Guoqiang Zhu, email: // Keywords : MUC4, carcinoma, epithelial, tumorigenicity Received : April 23, 2016 Accepted : October 26, 2016 Published : November 04, 2016 Abstract The cell surface membrane-bound mucin protein MUC4 promotes tumorigenicity, aggressive behavior, and poor outcomes in various types of epithelial carcinomas, including pancreatic, breast, colon, ovarian, and prostate cancer. This review summarizes the theories and findings regarding MUC4 function, and its role in epithelial carcinogenesis. Based on these insights, we developed an outline of the processes and mechanisms by which MUC4 critically supports the propagation and survival of cancer cells in various epithelial organs. MUC4 may therefore be a useful prognostic and diagnostic tool that improves our ability to eradicate various forms of cancer.

10.18632/oncotarget.13122

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Citations (45)