Rogério Silva Rosada

Biogen (Switzerland)

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Célio Lopes Silva

Universidade de São Paulo

Fabiani Gai Frantz

Universidade de São Paulo

Lucimara Gaziola de la Torre

Universidade Estadual de Campinas (UNICAMP)

Simone G. Ramos

Universidade de São Paulo

Arlete Aparecida Martins Coelho‐Castelo

Universidade de São Paulo

Lúcia Helena Faccioli

Universidade de São Paulo

Ana Paula Masson

Universidade de São Paulo

Izaíra Tincani Brandão

Universidade de São Paulo

Carlos R. Zárate-Bladés

Universidade Federal de Santa Catarina

Rodrigo F. Rodrigues

Universidade de São Paulo

Cooperative Institutions

Universidade de São Paulo

Universidade de Ribeirão Preto

Clinics Hospital of Ribeirão Preto

Universidade Estadual de Campinas (UNICAMP)

University of Michigan–Ann Arbor

Universidade Brasil

Delhi Pharmaceutical Science and Research University

Jamia Hamdard

Jadavpur University

Universidade Estadual Paulista (Unesp)

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The synergy between structural stability and DNA-binding controls the antibody production in EPC/DOTAP/DOPE liposomes and DOTAP/DOPE lipoplexes

Colloids and Surfaces B Biointerfaces (2009)

Lucimara Gaziola de la Torre Rogério Silva Rosada Ana Paula Fávaro Trombone Fabiani Gai Frantz Arlete Aparecida Martins Coelho‐Castelo

Naked DNA

Zeta potential

10.1016/j.colsurfb.2009.05.013

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Citations (37)

Experimental tuberculosis: Designing a better model to test vaccines against tuberculosis

Tuberculosis (2010)

Denise Morais da Fonseca Rogério Silva Rosada Marina Oliveira e Paula Pryscilla Fanini Wowk Luis H. Franco

10.1016/j.tube.2010.01.005

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Citations (21)

Protection against tuberculosis by a single intranasal administration of DNA-hsp65 vaccine complexed with cationic liposomes

BMC Immunology (2008)

Rogério Silva Rosada Lucimara Gaziola de la Torre Fabiani Gai Frantz Ana Paula Fávaro Trombone Carlos R. Zárate-Bladés

Abstract Background The greatest challenges in vaccine development include optimization of DNA vaccines for use in humans, creation of effective single-dose vaccines, development of delivery systems that do not involve live viruses, and the identification of effective new adjuvants. Herein, we describe a novel, simple technique for efficiently vaccinating mice against tuberculosis (TB). Our technique consists of a single-dose, genetic vaccine formulation of DNA-hsp65 complexed with cationic liposomes and administered intranasally. Results We developed a novel and non-toxic formulation of cationic liposomes, in which the DNA-hsp65 vaccine was entrapped (ENTR-hsp65) or complexed (COMP-hsp65), and used to immunize mice by intramuscular or intranasal routes. Although both liposome formulations induced a typical Th1 pattern of immune response, the intramuscular route of delivery did not reduce the number of bacilli. However, a single intranasal immunization with COMP-hsp65, carrying as few as 25 μg of plasmid DNA, leads to a remarkable reduction of the amount of bacilli in lungs. These effects were accompanied by increasing levels of IFN-γ and lung parenchyma preservation, results similar to those found in mice vaccinated intramuscularly four times with naked DNA-hsp65 (total of 400 μg). Conclusion Our objective was to overcome the significant obstacles currently facing DNA vaccine development. Our results in the mouse TB model showed that a single intranasal dose of COMP-hsp65 elicited a cellular immune response that was as strong as that induced by four intramuscular doses of naked-DNA. This formulation allowed a 16-fold reduction in the amount of DNA administered. Moreover, we demonstrated that this vaccine is safe, biocompatible, stable, and easily manufactured at a low cost. We believe that this strategy can be applied to human vaccines to TB in a single dose or in prime-boost protocols, leading to a tremendous impact on the control of this infectious disease.

Tuberculosis vaccines

Naked DNA

10.1186/1471-2172-9-38

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TB Vaccines: State of the Art and Progresses

Rogério Silva Rosada Rodrigo F. Rodrigues Fabiani Gai Frantz Frederico G. C. Arnoldi Lucimara Gaziola de la Torre

Tuberculosis vaccines

10.1007/978-3-7091-1419-3_13

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Citations (2)

REal-world effectiveness and safety of nataLIzumab in relApsing-remittiNg multiple sClerosis Brazilian patiEnts – RELIANCE study

María Fernanda Mendes Alfredo Damasceno Enedina Maria Lobato de Oliveira Ana Cláudia Piccolo Bianca Etelvina Santos de Oliveira

Natalizumab

Relapsing remitting

10.17648/bctrims-2023-167419

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The immune response to toxocariasis does not modify susceptibility to Mycobacterium tuberculosis infection in BALB/c mice.

PubMed (2007)

Fabiani Gai Frantz Rogério Silva Rosada Walter Miguel Turato Camila Matias Peres Arlete Aparecida Martins Coelho‐Castelo

Mycobacterium tuberculosis and helminth infections coincide geographically and are classically described as TH1 and TH2 pathologies. There is much interest in exploring how concurrent worm infections might alter immune responses to mycobacterial infection. To explore this issue, mice were infected with Toxocara canis and co-infected with M. tuberculosis. Mice infected with M. tuberculosis had high numbers of neutrophils and mononuclear cells within the alveolar spaces, with increased parenchymal interferon (IFN)-gamma levels. However, in Toxocara-infected mice we detected increased eosinophil numbers in bronchoalveolar lavage fluid (BALF) and increased parenchymal levels of interleukin (IL)-5. In co-infected mice the BALF demonstrated enhanced eosinophil influx with decreased neutrophil and mononuclear cell accumulation. However, co-infected mice had similar mycobacterial proliferation in their lungs accompanied by similar histopathological changes and similar cytokine/nitric oxide production compared with Mycobacterium-only-infected mice. Our results suggest that T. canis infection does not necessarily lead to increased susceptibility to pulmonary tuberculosis.

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5-lipoxygenase pathway is essential for the control of granuloma extension induced by Schistosoma mansoni eggs in lung

Experimental Parasitology (2016)

Gabriel Toffoli da Silva Milena S. Espíndola Caroline Fontanari Rogério Silva Rosada Lúcia Helena Faccioli

Proinflammatory cytokine

10.1016/j.exppara.2016.06.001

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A mathematical model describing the kinetic of cationic liposome production from dried lipid films adsorbed in a multitubular system

Brazilian Journal of Chemical Engineering (2007)

Lucimara Gaziola de la Torre A. L. Carneiro Rogério Silva Rosada Célio Lopes Silva Maria Helena Andrade Santana

Cationic liposomes are an alternative to DNA non-viral carrier in gene therapy, promoting higher efficiency for transport and delivery into the cells. Liposome production large scale is still a challenge. Among many processes, from dried film adsorbed inside multitubular system promising due its operational simplicity. this field, engineering contributions scarce no mathematical treatment literature describing type of process available. formulation proposed describe kinetic considering wetting disintegration lipid followed by lipids aggregation bilayers generating liposomes. model has fitted experimental data mass thickness, being useful predictions scaling up as well contributing development pharmaceutical products.

Cationic polymerization

10.1590/s0104-66322007000400002

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Citations (9)

Serum Amyloid P Therapeutically Attenuates Murine Bleomycin-Induced Pulmonary Fibrosis via Its Effects on Macrophages

PLoS ONE (2010)

Lynne A. Murray Rogério Silva Rosada Ana Paula Moreira Amrita Joshi Michael S. Kramer

Macrophages promote tissue remodeling but few mechanisms exist to modulate their activity during tissue fibrosis. Serum amyloid P (SAP), a member of the pentraxin family of proteins, signals through Fcgamma receptors which are known to affect macrophage activation. We determined that IPF/UIP patients have increased protein levels of several alternatively activated pro-fibrotic (M2) macrophage-associated proteins in the lung and monocytes from these patients show skewing towards an M2 macrophage phenotype. SAP therapeutically inhibits established bleomycin-induced pulmonary fibrosis, when administered systemically or locally to the lungs. The reduction in aberrant collagen deposition was associated with a reduction in M2 macrophages in the lung and increased IP10/CXCL10. These data highlight the role of macrophages in fibrotic lung disease, and demonstrate a therapeutic action of SAP on macrophages which may extend to many fibrotic indications caused by over-exuberant pro-fibrotic macrophage responses.

Alveolar macrophage

10.1371/journal.pone.0009683

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Citations (189)

Therapeutic Efficacy of Cintredekin Besudotox (IL13-PE38QQR) in Murine Lung Fibrosis Is Unaffected by Immunity to Pseudomonas aeruginosa Exotoxin A

PLoS ONE (2010)

Rogério Silva Rosada Ana Paula Moreira Fabiani Gai Frantz Raj K. Puri Aquilur Rahman

We have previously explored a therapeutic strategy for specifically targeting the profibrotic activity of IL-13 during experimental pulmonary fibrosis using a fusion protein comprised of human IL-13 and a mutated form of Pseudomonas aeruginosa exotoxin A (IL13-PE) and observed that the intranasal delivery of IL13-PE reduced bleomycin-induced pulmonary fibrosis through its elimination of IL-13-responsive cells in the lung. The aim of the present study was to determine whether the presence of an immune response to P. aeruginosa and/or its exotoxin A (PE) would diminish the anti-fibrotic properties of IL13-PE.Fourteen days after P. aeruginosa infection, C57BL/6 mice were injected with bleomycin via the intratracheal route. Other groups of mice received 4 doses of saline or IL13-PE by either intranasal or intraperitoneal application, and were challenged i.t. with bleomycin 28 days later. At day 21 after bleomycin, all mice received either saline vehicle or IL13-PE by the intranasal route and histopatological analyses of whole lung samples were performed at day 28 after bleomycin. Intrapulmonary P. aeruginosa infection promoted a neutralizing IgG2A and IgA antibody response in BALF and serum. Surprisingly, histological analysis showed that a prior P. aeruginosa infection attenuated the development of bleomycin-induced pulmonary fibrosis, which was modestly further attenuated by the intranasal administration of IL13-PE. Although prior intranasal administration of IL13-PE failed to elicit an antibody response, the systemic administration of IL13-PE induced a strong neutralizing antibody response. However, the prior systemic sensitization of mice with IL13-PE did not inhibit the anti-fibrotic effect of IL13-PE in fibrotic mice.Thus, IL13-PE therapy in pulmonary fibrosis works regardless of the presence of a humoral immune response to Pseudomonas exotoxin A. Interestingly, a prior infection with P. aeruginosa markedly attenuated the pulmonary fibrotic response suggesting that the immune elicitation by this pathogen exerts anti-fibrotic effects.

Pseudomonas exotoxin

Exotoxin

10.1371/journal.pone.0008721

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Citations (8)