Serum containing a monoclonal IgA protein from a patient with multiple myeloma gave intense immunofluorescent staining of smooth muscle fibres and the striations of skeletal muscle, cardiac muscle and thymic myoid cells. It also gave a weaker reaction with hepatocytes in a 'polygonal' pattern, and with renal glomeruli in a diffuse pattern. In culture fibroblasts, the serum stained long, parallel cytoplasmic filaments. Specificity of the staining reactions for actin was established by their prevention on serum absorption with skeletal muscle actin, but not by skeletal muscle myosin, tropomyosin or troponin, and by the demonstration that eluates obtained by acid dissociation of the serum-actin precipitates gave the same staining reactions as the original serum. Localization of the anti-actin reactivity to the monoclonal IgA protein was established by the observation that the same staining reactions were obtained with a monospecific IgA conjugate, with the eluate derived from the gamma globulin band of a serumel ectrophoretic strip, and with a purified euglobulin (IgA) fraction; also, the eluates obtained by acid dissociation of the serum actin precipitates contained monoclonal IgA.
Journal Article The Colonisation of Western Canada: A Study of Contemporary Land Settlement (1896–1934) Get access 26. The Colonisation of Western Canada: A Study of Contemporary Land Settlement (1896–1934). By Robert England, M.C., M.A. 1936. (London: P. S. King. 8vo. 341 pp. 15s.) F. Clarke F. Clarke Search for other works by this author on: Oxford Academic Google Scholar International Affairs, Volume 16, Issue 2, March-April 1937, Pages 294–296, https://doi.org/10.2307/2602507 Published: 01 March 1937
Abstract: Evaluating logging systems to determine which are most compatible with the maintenance of biodiversity is of prime importance if tropical forests are to be managed in a sustainable way. Bats are model taxa for this purpose. Two different logging systems are used in the natural forest of the Victoria‐Mayaro Forest Reserve in Trinidad: open range and periodic block. Open range is a continuous harvesting system and, in common with most methods used to log tropical forests worldwide, has few harvest controls other than girth limits for selected species. Periodic block is a polycyclic system, with felling based on ecological criteria assumed to be compatible with the maintenance of biodiversity. To compare the effects of periodic block and open‐range systems on biodiversity, we determined bat species richness and abundance in each system and in primary forest. We caught bats in mist nets set at ground level and in the canopy and in harp traps. In total 1959 individuals representing 38 species were captured. Species richness was similar among primary forest and logged forest habitats, although bat diversity was lower in logged forest. The distributions of bat species abundance did not differ significantly between logged forest and primary forest. We found, however, that both logging systems lead to a decrease in gleaning animalivores and an increase in frugivores. The increase in frugivores was likely the result of an increase in the abundance of bat‐dispersed pioneer fruiting plants in logged forest. Bats of periodic‐block‐managed forest were more similar to those of primary forest than those of forest logged using the open‐range system, indicating that the periodic‐block system is more compatible with the maintenance of bat diversity. Our results support the suggestion that the measured use of tropical forests can largely be compatible with biodiversity conservation.
The interaction of fructose diphosphate aldolase with F-actin, F-actin-tropomyosin, and F-actin-tropomyosin-troponin has been studied by using negative staining. In the absence of troponin, minor aggregates of aldolase and the F-actin filaments are formed. A well-ordered lattice structure is only formed in the case of the fully reconstituted filament when the filament-to-filament spacing is 18nm, and the cross-bridge spacing is 38.7 nm. Evidence is presented that the lattice is due to an interaction between troponin and aldolase. The minimum subunit structure of troponin, still capable of giving rise to a lattice, is the troponin-IT complex, which indicates that troponin-C is not involved in aldolase binding.
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