James H. Pringle

University of Leicester

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Co-Authors

I Lauder

University of Hong Kong

Jacqui Shaw

University of Leicester

Gerald Saldanha

Leicester Royal Infirmary

Catrin Pritchard

University of Leicester

Marion MacFarlane

MRC Toxicology Unit

Linda Potter

University of Leicester

Robert A. Goodlad

The Honourable Society of Lincoln's Inn

Philip East

The Francis Crick Institute

Stuart McDonald

Queen Mary University of London

Anita Milicic

Jenner Institute

Cooperative Institutions

University of Leicester

162

Leicester Royal Infirmary

115

University Hospitals of Leicester NHS Trust

Leicester General Hospital

Cancer Research UK

Newcastle University

University College London

King's College London

University of Newcastle Australia

Ollscoil na Gaillimhe – University of Galway

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MicroRNA-21 expression and its pathogenetic significance in cutaneous melanoma

Melanoma Research (2016)

Gerald Saldanha Linda Potter Yee Shin Lee Sophie Watson Priya Shendge

Identification of prognostic biomarkers is timely for melanoma as clinicians seek ways to stratify patients for molecular therapy. MicroRNAs are promising as tissue biomarkers because they can be assayed directly from formalin-fixed paraffin-embedded clinical samples. We previously reported that microRNA-21 (miR-21) was strongly expressed in melanoma relative to naevi and now sought to further assess the significance of this by assessing its relationship with its putative target, PTEN. Clinical melanoma samples were analysed by immunohistochemical analysis for PTEN, stem–loop qRT-PCR for miR-21 and PCR for BRAF/NRAS mutation status. Cell lines were investigated for the effect of anti-miR-21 on PTEN. A total of 81 clinical melanocytic tumour samples were investigated, with uniformly high PTEN expression in the nucleus and cytoplasm of naevi and with preferential loss of PTEN expression in the nucleus of melanoma cells. miR-21 expression was inversely associated with nuclear PTEN expression but not with cytoplasmic PTEN expression. An anti-miR-21 preferentially altered nuclear PTEN in melanoma cell lines. The presence of a BRAF or NRAS mutation had no significant effect on miR-21 expression. These data suggest miR-21 may exert an oncogenic effect in melanoma by favouring redistribution of PTEN to the nucleus.

Clinical Significance

10.1097/cmr.0000000000000216

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Citations (21)

ReactiveVersusNeoplastic Monocytoid B-Cell Proliferations:In Situ Hybridization Study of Immunoglobulin Light Chain mRNA

American Journal of Clinical Pathology (1995)

Lukáš Plank Karen Hell Martin‐Leo Hansmann James H. Pringle I Lauder

To distinguish reactive versus neoplastic monocytoid B-cell (MBC) proliferations, the clonality of MBC was examined in paraffin-embedded tissues by in situ hybridization (ISH) of immunoglobulin (Ig) light chain messenger RNA (mRNA) with sensitive oligonucleotide probes in 26 cases. They included 13 cases of lymphadenitis with MBC reaction and 13 cases of nodal (n = 8) and extranodal (n = 5) monocytoid B-cell lymphoma (MBCL). Two cases represented a composite lymphoma showing a centroblastic-centrocytic and MBCL component. The clonality of M BC infiltrates could be demonstrated in 16 of 26 (61.5%) cases by immunostaining for Ig light chains and in all (100%) cases by ISH. Neoplastic MBC usually expressed a faint-to-moderate light chain restriction of mRNA, whereas some MBC (10% to 30% of total MBCL population) showed a strong positivity irrespective of plasmacytoid differentiation as indicated by Ig immunostaining (present in 9 of 13 cases). Reactive MBC expressed a faint κ and λ light-chain mRNA positivity. Five percent to 20% of total reactive MBC showed also a strong positivity for both Ig light chain mRNA, although only a minor part of these cells (7 of 13 cases) expressed polyclonal Ig by immunohistochemistry. These results indicate that (1) both reactive and neoplastic MBC can differentiate into plasma cells; and (2) a relatively high percentage of reactive and neoplastic MBC show a detectable mRNA transcription, but not a corresponding Ig synthesis. Either the Ig detection is not sensitive enough or these cells might be in an early differentiation phase, where the Ig production has not yet started.

Immunoglobulin heavy chain

10.1093/ajcp/103.3.330

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Citations (15)

Absence of Epstein-Barr virus RNA in multiple sclerosis as assessed by in situ hybridisation.

Journal of Neurology Neurosurgery & Psychiatry (1994)

David A. Hilton Seth Love A. Fletcher James H. Pringle

Epidemiological and serological evidence has suggested a role for Epstein-Barr virus infection in the aetiology of multiple sclerosis. Epstein-Barr virus-specific RNA was looked for in the brains of 10 patients with multiple sclerosis by in situ hybridisation. A total of 21 plaques was examined. In all of these preservation of RNA was shown by hybridisation of control probes to mitochondrial rRNA but no signal was detected with the Epstein-Barr virus probes. It is unlikely that persistent or latent Epstein-Barr virus infection of the CNS occurs in multiple sclerosis, although present findings do not exclude a role for Epstein-Barr virus in the initiation of this disorder.

Epstein–Barr virus infection

Etiology

Gammaherpesvirinae

RNA virus

10.1136/jnnp.57.8.975

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Citations (58)

Correction to: Association of invasion-promoting tenascin-C additional domains with breast cancers in young women

Breast Cancer Research (2018)

David S. Guttery Rachael A. Hancox Kellie Mulligan Simon Hughes Sinéad Marie Lambe

After the publication of this work [1] an error was noticed in Fig. 6 (b). In the MCF-7/Vector columns, the same image was used accidentally for the 0 h and 24 h time points. Both images were taken from the 0 h time point.

Surgical oncology

Association (psychology)

10.1186/s13058-018-0997-8

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Citations (0)

The unfavorable effect of the A allele of the vitamin D receptor promoter polymorphism A-1012G has different mechanisms related to susceptibility and outcome of malignant melanoma

Dermato-Endocrinology (2009)

John A. Halsall Joy Osborne Michael P. Epstein James H. Pringle P.E. Hutchinson

The A allele of the A-1012G (rs4516035) vitamin D receptor (VDR) promoter polymorphism is associated with increased susceptibility and worsened outcome in malignant melanoma (MM). The A allele contains a GATA-3 binding site. There is a second polymorphism in the same promoter region, G-1520C (rs7139166), and there is potential for another GATA binding site in the G allele. Here, we tested the hypothesis that the G-1520A-1012 haplotype might be a greater risk factor for MM than A-1012 alone. The A allele of A-1012G was preferentially linked to G of G-1520C and was more frequent in MM patients (P=0.011) but G of G-1520C was not (P=0.756). The CA haplotype was a very significant risk factor for MM (P=0.0001) while the CG haplotype was protective (P=0.014, combined model P=0.00002). There was no effect of GA haplotype (P=0.931), suggesting that that the difference in frequencies of the A allele between patients and controls was accounted for by the differences in frequencies of the CA haplotype. The A allele of A-1012G was more frequent in patients with metastasis (P=0.054) than MM patients without metastasis, as was the G allele of G-1520C (P=0.028). The GA haplotype was more frequent in patients with metastasis (P=0.015), while frequencies of CA were similar. We suggest that the different roles of the A allele of A-1012G in susceptibility and metastasis risk may be a function of the availability of transcription factors in the differing cellular backgrounds related to susceptibility and progression of MM.

10.4161/derm.1.1.7674

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Citations (29)

Accurate Detection of Copy Number Changes in DNA Extracted from Formalin-Fixed, Paraffin-Embedded Melanoma Tissue Using Duplex Ratio Tests

Journal of Molecular Diagnostics (2013)

David A. Moore Gerald Saldanha Abdlrzag Ehdode Linda Potter Lovesh Dyall

Duplex (building)

10.1016/j.jmoldx.2013.04.008

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Citations (8)

Supplementary Materials from Ectopic Expression of P-Cadherin Correlates with Promoter Hypomethylation Early in Colorectal Carcinogenesis and Enhanced Intestinal Crypt Fission <i>In vivo</i>

Anita Milicic Lea–Anne Harrison Robert A. Goodlad Robert G. Hardy Anna M. Nicholson

Supplementary Materials from Ectopic Expression of P-Cadherin Correlates with Promoter Hypomethylation Early in Colorectal Carcinogenesis and Enhanced Intestinal Crypt Fission <i>In vivo</i>

Ectopic expression

Aberrant crypt foci

10.1158/0008-5472.22374756

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Increased dimeric IgA producing B cells in the bone marrow in IgA nephropathy determined by in situ hybridisation for J chain mRNA.

Journal of Clinical Pathology (1996)

Steven J. Harper Alice C. Allen James H. Pringle John Feehally

AIM: To investigate the possible role of the systemic IgA immune system in the pathogenesis of IgA nephropathy METHODS: J chain mRNA expression in the IgA cells of the bone marrow was studied. Bone marrow trephine biopsy specimens from seven patients with IgA nephropathy and seven matched controls were examined by (1) non-isotopic in situ hybridisation (ISH) and (2) combined immunofluorescence and non-isotopic ISH to identify the plasma cell type. Serum polymeric IgA was also determined using standard high pressure liquid chromatography and sandwich enzyme linked immunosorbent assay. RESULTS: Non-isotopic ISH revealed a similar number of J chain mRNA positive cells/unit length in biopsy specimens from patients (16.5 +/- 2.7 cells/mm) and controls (17.7 +/- 2.4 cells/mm). Combined immunofluorescence and ISH revealed a greater proportion of J chain mRNA positive IgA cells in patients (7.6 +/- 1.45%) compared with controls (3 +/- 0.8%). Serum polymeric IgA was similar in both patients (91 +/- 22 mg/l) and controls (77 +/- 24 mg/l). CONCLUSION: These data suggest that excess production of dimeric IgA occurs in the bone marrow in IgA nephropathy.

Immunofluorescence

J chain

Immunoglobulin A

Pathogenesis

10.1136/jcp.49.1.38

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Citations (106)

High‐grade T‐cell lymphoma following treatment with cyclosporin A

Histopathology (1991)

Lawrence A. Brown Martin Wiselka A. C. Campbell James H. Pringle Karl G. Nicholson

A 47‐year‐old man with persistent severe oropharyngeal ulceration developed a high‐grade T‐cell lymphoma soon after commencing treatment with cyclosporin A. Using Southern blotting to identify T‐cell β ‐chain gene rearrangements, evidence of clonal restriction was found both in blood and lymph node DNA samples. Two BamH1 rearranged bands were demonstrated in both samples. In the blood a 16 Kb band predominated, with a weaker 28 Kb band. In the lymph node sample this pattern was reversed. The findings suggest that a bi‐clonal population of T‐lymphocytes or clonal evolution of an existing T‐cell monoclone had developed, and that cyclosporin contributed to the emergence of a high‐grade T‐cell lymphoma.

Southern blot

T-Cell Lymphoma

Hematology

10.1111/j.1365-2559.1991.tb00026.x

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Citations (4)

Non-isotopic detection of RNA in situ

Oxford University Press eBooks (1995)

James H. Pringle

Abstract The synthesis of RNA plays a crucial role in the molecular biology of the cell and is, therefore, an important process to study. Although RNA can provide a source of genetic information in the genome of RNA viruses, its main role in the cell is to aid the transfer of genetic information from genomic DNA to functional proteins during gene expression. The process is regulated at each step in the pathway from DNA to RNA to protein (1). This includes the control of transcription of heterogeneous nuclear RNA (hnRNA), hnRNA processing to produce mature mRNA via the spliceosomes, mRNA transportation to the cytoplasm, and mRNA translation into proteins. The majority of cellular RNAs including tRNAs, rRNAs, and small nuclear RNAs (snRNA) have catalytic functions in this process (2, 3).

Small nuclear RNA

Precursor mRNA

Transcription

RNA Silencing

10.1093/oso/9780199634552.003.0004

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Citations (3)