Fuzhu Liu

Northwest A&F University

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Zhuye Niu

Northwest A&F University

Wenzong Lu

Xi'an Technological University

Qingwang Li

Northwest A&F University

Xiaolei Yang

Chongqing Medical University

Zengsheng Han

Yanshan University

Guoxia Geng

Northwest A&F University

Qiuliang Yan

Jilin Academy of Agricultural Sciences

Jian Li

Beijing Chao-Yang Hospital

Qingwang Li

Northwest A&F University

Shi Jingsong

Cooperative Institutions

Northwest A&F University

Yanshan University

Chinese Academy of Agricultural Sciences

Central South University

University of Chinese Academy of Sciences

State Key Laboratory of Metastable Materials Science and Technology

Institute of Animal Sciences

Sichuan University

Capital Medical University

Sun Yat-sen University

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Crude flavonoids from Carya cathayensis Sargent inhibited HeLa cells proliferation through induction of apoptosis and cell cycle arrest

Latin American Journal of Pharmacy (2009)

Wenzong Lu Yali Yang Qingwang Li Fuzhu Liu

SUMMARY. The aim of this study was to investigate the anticancer properties of crude flavonoids from Carya cathayensis Sargent bark (CCS-F), including the inhibitory effect of CCS-F on HeLa cells and the apoptosis-inducing capacity in vitro. In our results, treatment of HeLa cells with CCS-F resulted in the growth inhibition effect, and the IC50 was 95 μg/ml. Detection of apoptosis was performed by acridine orange / ethidium bromide (AO/EB) and Tdt-mediated dUTP nick end labeling (TUNEL) staining assays, which showed more apoptosis cells in CCS-F treatment group than the control group. Furthermore, CCSF (100 μg/ml) could arrest the cells in G0/G1 phase. Meanwhile, the expression of Bax was increased in the cells treated with CCS-F (100 μg/ml), with an increase in the activity of caspase-3, while Bcl-2 expression was decreased compared to the control cells. It demonstrated that CCS-F had antiproliferative activity in HeLa cells and might be a potential anticancer drug.

HeLa

Acridine orange

Ethidium bromide

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Citations (3)

Effects of different levels of selenium on growth performance and immunocompetence of broilers under heat stress

Archives of Animal Nutrition (2009)

Zhuye Niu Fuzhu Liu Qiuliang Yan Long Li

An experiment was undertaken to evaluate the effect of dietary selenium (Se) levels on growth performance and immune competence of broilers under heat stress. Birds were raised in either a thermoneutral (TN, 23.9 degrees C constant) or heat stress conditions (HS, 23.9 degrees C to 38 degrees C cycling) and were fed a corn-soybean meal basal diet supplemented with Se at 0, 0.2 and 0.4 mg/kg. A total of 240 one-day-old male broiler chicks were randomly assigned to six groups; each group had four replicates of 10 birds. Body weight and feed intake were not influenced by dietary Se, while feed conversion was significantly improved by a Se-supplementation of 0.2 mg/kg. HS significantly reduced body weight, feed intake and feed conversion. Numbers of abdominal exudate cells (AEC), percentage of macrophages in AEC, phagocytic macrophages, internalized opsonised and unopsonised sheep red blood cells (SRBC) were significantly increased by dietary Se. Both primary and secondary antibody responses were characterised by increasing titres of antibody to SRBC by dietary Se when birds were exposed to HS (p < 0.05). Lymphoid organ weights, antibody responses, incidence of macrophages in AEC, and phagocytic ability of macrophages were also significantly reduced under HS. These results indicated that HS severely reduced growth performance and immunocompetence of broilers, whereas the immune response of broilers improved by dietary Se supplementation under HS.

Immunocompetence

10.1080/17450390802611610

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Citations (79)

Carcass Quality of Different Meat - Typed Chickens When Achieve a Common Physiological Body Weight

International Journal of Poultry Science (2008)

Fuzhu Liu Zhuye Niu

This experiment was conducted to compare carcass characteristics of native quality chicken white Lueyang (WL), modern commercial broiler strain Arbor Acres breeder (AA) and their respective F cross when 1 achieve a common live body weight. A total of 300 day-old chicks were allotted to three groups and reared under the same conditions until they reached market weight (1800±20 g) respectively, then ten of individual male birds from each group were killed for measuring. There were significant differences in carcass and meat characteristics for breeds at a common market weight. WL had a large age to market weight, lower abdominal fat weight, lower breast meat yield, higher leg meat yield (P<0.05 or P<0.01). moisture and lipids of both breast muscle and thigh muscle were lower for WL than AA (P<0.01), but protein and ash components of both breast muscle and thigh muscle were higher for WL than AA (P<0.05 or P<0.01). Muscle pH was higher for WL than AA (P<0.05). WL had a lower L* value than AA and their F (P<0.05), but a higher 24h 1 a* value and b* value (P<0.01). The F crosses appeared to have characteristics more like AA for carcass 1 and meat characteristics.

Leg muscle

Carcass weight

Abdominal fat

Abdominal muscles

10.3923/ijps.2008.319.322

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Citations (17)

Vitamin C and vitamin E supplementation alleviates oxidative stress induced by dexamethasone and improves fertility of breeder roosters

Animal Reproduction Science (2016)

Yuna Min Tong-Tong Sun Zhuye Niu Fuzhu Liu

Malondialdehyde

Semen quality

10.1016/j.anireprosci.2016.04.005

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Citations (48)

Antitumor activity of polysaccharides isolated fromPatrinia heterophylla

Pharmaceutical Biology (2010)

Wenzong Lu Guoxia Geng Qingwang Li Jian Li Fuzhu Liu

The research investigated the effect of Patrinia heterophylla Bunge (Valerianaceae) polysaccharides (PHB-P1) on U14-bearing mice. The tumor weight of mice treated with PHB-P1 (30, 60 mg/kg body weight) was significantly lower than that of the control group, a decrease of serum lactate dehydrogenase (LDH) activity was observed, and the serum alkaline phosphatase (AKP) level was increased slightly. The number of apoptotic tumor cells was significantly increased in the mice by treatment of PHB-P1 (30, 60 mg/kgbw). Cell cycle analysis showed the accumulation of tumor cells in the G2/M phase and a relative decrease of the S phase. By the immunohistochemical analysis, PHB-P1 (30, 60 mg/kgbw) might up-regulate the expression of p53 and Bax, and significantly inhibited the expression of Bcl-2 in tumor tissues. In conclusion, PHB-P1 could inhibit tumor growth and induce tumor cell apoptosis.

10.3109/13880200903437852

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Citations (18)

A Bioassay Method for Metabolizable Energy of Feedstuffs for Peking Ducks

International Journal of Poultry Science (2008)

Zhuye Niu Chunlei Zhang Fuzhu Liu

Three experiments were carried out to determine optimal feed input and excreta collection time in bioassay of feedstuff true metabolizable energy for ducks. In experiment 1, 20 drakes had been food withdrawn and were allocated to 4 groups, each of which contained 5 birds. Birds of each group were force- fed 50 g complete feed, approximately 48 h after food withdrawal. The excreta voided during the exact 56 h following feeding were collected. In experiment 2, 28 drakes were force-fed 50 g complete feed, approximately 48 h after food withdrawal. 4 birds were killed by an intravenous dose of sodiu m pentobarbitone 4 h, 8 h, 14 h, 24 h, 32 h, 38 h and 48 h after feeding. The residues in alimentary canal were collected and dried in an oven at 65 C. In experiment 3, 70 mature drakes were allocated to 7 groups. The o treatments comprised an unfed negative control and five levels of feed input (30, 50, 70, 90, 110 and 150 g complete feed). The birds were force-fed at approximately 48 h after food withdrawal. The excreta voided during the exact 36 h following feeding were collected. The results indicated that the time prior to force - feeding and excreta collection period should be 32-36 h when evaluating feed metabolizable energy and 50- 70 g feed per bird is feasible for evaluating feed metabolizable energy.

Animal Feed

10.3923/ijps.2008.884.886

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Citations (1)

Influence of Paprika Extract Supplement on Egg Quality of Laying Hens Fed Wheat-Based Diet

International Journal of Poultry Science (2008)

Zhuye Niu Jin Fu Yupeng Gao Fuzhu Liu

This study was conducted to evaluate the effect of paprika extract (PE) on egg performance and egg quality of laying hens. A total of 960 fifty-three wk-old laying hens were randomly allotted into five groups and each of six replicates with 32 birds. The birds were fed wheat-soybean meal-based diets supplemented with 0, 0.1%, 0.2%, 0.4% and 0.8% PE respectively. The experiment lasted 4 weeks. RFC value linearly increased with an increase in dietary PE (P < 0.01) and achieved 9.9 when dietary PE added by 0.8%. L* value of egg yolk decreased with increase in dietary PE (P < 0.05), The a* value linearly increased with an increase in dietary PE (P < 0.01), while b* value unaffected (P<0.05). There were no effect of dietary PE on Haugh unit and cholesterol content in yolk (P<0.05). However, carotenoid content in yolk linearly increased with an increase in dietary PE (P < 0.01). No differences were observed in egg size, laying rate, feed intake and feed conversion among dietary treatments (P<0.05). The results indicated supplementation of paprika extract significantly improved egg yolk color when hens fed wheat-based diet and yolk coloration could reach an ideal grade when paprika extract added by 0.8%.

Laying

10.3923/ijps.2008.887.889

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Citations (28)

Polysaccharide fromPatrinia heterophyllaBunge Inhibits HeLa Cell Proliferation Through Induction of Apoptosis and Cell Cycle Arrest

Laboratory Medicine (2009)

Wenzong Lu Qingwang Li Jian Li Fuzhu Liu Xiaolei Yang

BackgroundTraditional Chinese medicine was used to provide the novel basis for pharmaceutical drug discovery. In recent years, polysaccharides isolated from traditional Chinese medicinal herbs were widely studied to find an additional treatment for cancer therapy. Patrinia heterophylla Bunge usually functions as a folklore medicine against leucorrhea, metrorrhagia, and metrostaxis. The bioactive compounds of Patrinia heterophylla Bunge, such as "polysaccharide," were found to possess potent anticancer activity.

HeLa

MTT assay

10.1309/lm9tyo4wn0derurf

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Citations (9)

Anti-Tumor Activity of Polysaccharides Isolated from Patrinia scabra Bunge on U14 Cervical Carcinoma Bearing Mice

The American Journal of Chinese Medicine (2009)

Wenzong Lu Guoxia Geng Qingwang Li Jian Li Fuzhu Liu

The aim of our study was to investigate the effect of Patrinia scabra Bunge polysaccharide (PSB-P2) on cervical cancer cell (U14)-bearing mice. The tumor weight of mice treated with PSB-P2 (40, 80 mg/kg b.w.) was significantly lower than that of the control group and serum lactate dehydrogenase (LDH) activity was decreased, while serum alkaline phosphatase (AKP) level was only changed slightly. Meanwhile, the number of apoptotic tumor cells was significantly increased in the mice by the treatment of PSB-P2 (40, 80 mg/kg b.w.). At the same time, cell cycle analysis showed the accumulation of tumor cells in the G0/G1 phase and a relative decrease in the S phase. On the other hand, using the reverse transcription-polymerase chain reaction (RT-PCR) assay, PSB-P2 (40, 80 mg/kg b.w.) showed the up-regulation of p53 and Bax, and significant inhibition of Bcl-2 in tumor tissues. It suggests a possible mechanism of the inhibitory effect of PSB-P2 on tumor growth.

10.1142/s0192415x09007429

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Citations (14)

Effects of Dietary Energy and Protein on Growth Performance and Carcass Quality of Broilers during Starter Phase

International Journal of Poultry Science (2009)

Zhuye Niu Shi Jingsong Fuzhu Liu Xianhui Wang Chun‐qi Gao

A 3x4 factorial arrangement with three Metabolizable Energy (ME) levels (12.13, 12.55, 12.97 MJ/kg) and four Crude Protein (CP) levels (20.0, 21.0, 22.0 and 23.0%), respectively, was undertaken to investigate the influence of varying levels of dietary energy and protein on broiler performance and carcass quality from 1-21 d aysof age. Six hundred 1 day old broiler chicks were randomly divided into 12 treatments, each of which had five replicates of 10 birds. BW of 21 days of age was significantly increased with an increase in dietary ME (p 0.05). Both ME and CP significantly improved feed efficiency (P<0.05). ME significantly affected on feed intake, while CP not affected. However, there were no significant interaction in BW, average daily gain, feed intake and feed efficiency between dietary ME and CP. Higher level of dietary ME (12.97 MJ/kg) significantly increased abdominal fat percentage when compared with lower ME (12.13MJ/kg or 12.55 MJ/kg). L* of leg meat was decreased by dietary ME, while L* of breast meat was not affected. a* of both breast meat and leg meat was increased with increasing dietary ME and CP. b* of leg meat was decreased by ME while b* of breast meat was not affected. Water-holding capacity (WHC) of breast meat was decreased by dietary ME, while increased by dietary CP. The results of present research indicated that the optimal dietary ME requirement of broilers from 1-21 days of age is 12.97 MJ/kg and the CP requirement is 21-22%.

Starter

10.3923/ijps.2009.508.511

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Citations (42)