T. A. Batanova

Gifu University

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Yasuhiro Takashima

Gifu University

Katsuya KITOH

Gifu University

Nina V. Tikunova

Siberian Branch of the Russian Academy of Sciences

МП

М. П. Кирпичников

Institute of Bioorganic Chemistry

L. S. Sandakhchiev

State Research Center of Virology and Biotechnology VECTOR

L. N. Shingarova

Institute of Bioorganic Chemistry

E. F. Boldyreva

Institute of Bioorganic Chemistry

АА

А. А. Панина

Volgograd State Medical University

ТК

Т. К. Алиев

Moscow State University

A. A. Ilyichev

State Research Center of Virology and Biotechnology VECTOR

Cooperative Institutions

West Park Hospital

Hannah Research Foundation

Gifu University

Russian Academy of Sciences

State Research Center of Virology and Biotechnology VECTOR

The University of Tokyo

Universidade de São Paulo

University of Bern

Institute of Bioorganic Chemistry

University of Copenhagen

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Human single-chain antibodies specific to the tumor necrosis factor

Russian Journal of Bioorganic Chemistry (2011)

M. A. Vikhrova T. A. Batanova Л. Р. Лебедев L. N. Shingarova Ludmila A. Frank

Bioorganic chemistry

Single-chain variable fragment

Bovine serum albumin

10.1134/s1068162011030198

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Citations (1)

Recombinant full-size human antibody to Ebola virus

Russian Journal of Bioorganic Chemistry (2007)

L. N. Shingarova Nina V. Tikunova Tatyana Yun А. А. Чепурнов Т. К. Алиев

Nucleoprotein

10.1134/s1068162007060040

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Citations (5)

Cell Surface Expression of a Chimeric Protein Containing Mouse Immunoglobulin G1 Fc Domain and its Immunological Property

Journal of Veterinary Medical Science (2006)

T. A. Batanova Haruko Ota Katsuya KITOH Yasunobu Matsumoto Yoshihiro Hayashi

Recently we reported that a chimeric molecule containing mouse transferrin receptor and immunoglobulin G1 (IgG1) Fc, mTR-Fc, induced higher immune responses and can be used as a vaccine adjuvant. In this study, the immunological property of the molecule was investigated. Although, the mTR-Fc did not activate complement classical pathway, it was recognized by activated macrophage as like intact IgG Fc, which is recognized by macrophage via Fcγ receptor. In addition, we found that splenocyte simultaneously exposed to lipopolysaccaride (LPS) and mTR-Fc produced higher amount of interleukin-10, comparing to that exposed to only LPS. These results suggest that the mTR-Fc molecules conserved the IgG Fc property to biasing immune responses via modulation of cytokine production by antigen presenting cell.

Splenocyte

Fragment crystallizable region

Fc receptor

Immunoglobulin Fc Fragments

Transferrin receptor

10.1292/jvms.68.87

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Citations (1)

РЕКОМБИНАНТНОЕ ПОЛНОРАЗМЕРНОЕ АНТИТЕЛО ЧЕЛОВЕКА ПРОТИВ ВИРУСА ЭБОЛА

Биоорганическая химия (2007)

L. N. Shingarova Н.В. Тикунова Tatarinova IuN Aleksandr A. Chepurnov Т. К. Алиев

Для конструирования полноразмерного антитела человека против вируса Эбола гены, кодирующие константные домены тяжелой и легкой цепей иммуноглобулина человека, были объединены с соответствующими фрагментами ДНК, кодирующими вариабельные домены одноцепочечного антитела 4D1, специфичного к вирусу Эбола, отобранного из комбинаторной фаговой библиотеки одноцепочечных антител человека. Были созданы две экспрессионные плазмиды pCH1 и pCL1, содержащие искусственные гены, кодирующие тяжелую и легкую цепи иммуноглобулина человека соответственно. Их котрансфекция в клеточную линию эмбриональных почек человека HEK293Т обеспечила продукцию полноразмерного рекомбинантного антитела человека. Константа аффинности этого антитела, измеренная с помощью твердофазного ИФА, составила 7.7 ? 107 ± 1.5 ? 107 М-1. Полученное антитело, как и исходное одноцепочечное антитело 4D1, связывало нуклеопротеин NP вируса Эбола, но не взаимодействовало с белками вируса Марбург.

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Toxoplasma gondii Seroprevalence in Domestic Animals and Humans in Mymensingh District, Bangladesh

Journal of Veterinary Medical Science (2011)

M Shahiduzzaman MR Islam Most. Monjila KHATUN T. A. Batanova Katsuya KITOH

The seroprevalence of Toxoplasma gondii was examined in adult women and domestic animals used for meat products from the Mymensingh District, Bangladesh. Cattle, goats and sheep showed a high seroprevalence (12, 32 and 40%, respectively), while the sera from all fifteen women examined in the same area were seronegative. Considering that primary infection in women during pregnancy can cause abortion and congenital defects, accidental ingestion of T. gondii infected meat products from domestic animals represents a risk factor for adult women living in the same area.

Seroprevalence

Toxoplasmosis

10.1292/jvms.11-0084

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Citations (28)

Toxoplasma gondii tachyzoite-infected peripheral blood mononuclear cells are enriched in mouse lungs and liver

Experimental Parasitology (2013)

Akihiro Unno Seira Kachi T. A. Batanova Tamio Ohno Nagwa M. Elhawary

10.1016/j.exppara.2013.03.006

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Citations (26)

Phage antibodies from combinatorial library neutralize vaccinia virus

Human Antibodies (2001)

Nina V. Tikunova Vera V. Morozova T. A. Batanova Е. Ф. Беланов Н. И. Бормотов

The library of human scFv antibodies displayed on the surface of bacteriophages was panned against Vaccinia virus (VACV), strain Elstree. 75% binding with Vaccinia virus. 5 clones were characterized for their binding with VACV and their ability to ne

Orthopoxvirus

Poxviridae

10.3233/hab-2001-103-401

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Citations (5)

Adhesion of Toxoplasma gondii tachyzoite-infected vehicle leukocytes to capillary endothelial cells triggers timely parasite egression

Scientific Reports (2017)

Minami Baba T. A. Batanova Katsuya KITOH Yasuhiro Takashima

Intracellular pathogens have numerous strategies for effective dissemination within the host. Many intracellular pathogens first infect leukocytes, which they use as a vehicle to transport them to target organs. Once at the target organ, intracellular parasite Toxoplasma gondii can cross the capillary wall in extracellular form by infecting endothelial cells. However, after egression from leukocytes, extracellular parasites face the risk of host immune attack. In this study, observation of infected mouse organs, using a method that renders tissue transparent, revealed that adhesion of tachyzoite-infected leukocytes to endothelial cells triggers immediate egression of the parasite. This signal enables the parasite to time egression from its vehicle leukocyte to coincide with arrival at a target organ, minimizing the opportunity for immune attack during the transition from a vehicle leukocyte to capillary endothelial cells.

Intracellular parasite

10.1038/s41598-017-05956-z

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Citations (25)

Prevalence and Dynamics of Antibodies against NcSAG1 and NcGRA7 Antigens of <i>Neospora caninum</i> in Cattle during the Gestation Period

Journal of Veterinary Medical Science (2013)

Yasuhiro Takashima Masaki Takasu Isao YANAGIMOTO Naoki Hattori T. A. Batanova

Bovine abortion caused by the Apicomplexan parasite Neospora caninum is a major economic problem in the livestock industry worldwide. Our study measured the prevalence and temporal changes in levels of antibodies specific for two N. caninum derived antigens, NcSAG1 and NcGRA7, to determine an appropriate strategy for serodiagnosis. Using an enzyme-linked immunosorbent assay (ELISA), blood samples showed that 71 cows out of 129 were positive for anti-NcSAG1 antibodies and that only nine cows were positive for anti-NcGRA7 antibodies. By longitudinal sampling, it was revealed that positive and negative antibody conversion occurred frequently for anti-NcGRA7, but that anti-NcSAG1 antibodies persisted for a long-term. These results indicate the usefulness of measuring anti-NcSAG1 antibody levels for the detection of chronically infected cows. Twelve cows showed positive seroconversion during pregnancy, nine of which showed seropositivity for anti-NcGRA7 antibody at the sixth and/or seventh month of pregnancy; serum samples were not obtained from the remaining three cows during this period. Therefore, the optimal time for detection of anti-NcGRA7 antibodies appears to be between the fifth and eighth month of pregnancy.

Seroconversion

Neospora

Gestation period

10.1292/jvms.13-0198

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Citations (18)

Suppression of inflammatory genes expression in the injured host intestinal wall during Mesocestoides vogae tetrathyridium larvae migration

PLoS neglected tropical diseases (2020)

Kei Hayashi Rinako Sugisawa Taizo Saito Toshiyasu Matsui Yuji Taniguchi

Mesocestoides vogae is a cestode parasite of the family Mesocestoididae (order Cyclophyllidea). Its larvae, tetrathyridium, are approximately 1 mm long and 300 μm wide and infect a wide range of host species including humans. Tetrathyridium migrate through the intestinal wall to invade the peritoneal cavity. Despite intestinal penetration by such a large-sized parasite, symptomatic intestinal disorders are not common during the migration period. In this study, the dynamics of tetrathyridia migration and their pathogenicity towards intestinal tissues were examined in mice infected orally with these parasites. Most tetrathyridia were found to migrate through the intestinal wall, moving into the peritoneal cavity or liver 24 to 48 hours after the oral infections. Next, the pathogenicity of tetrathyridium in the intestinal wall was histopathologically evaluated, and tissue injury from tetrathyridium migration was confirmed. Inflammatory foci were observed as tetrathyridium migration tracks from 48 hours after oral infection; however, the number of inflammatory foci had decreased by half more than 48 hours later. Therefore, we examined the gene expression levels of the macrophage driving cytokine, IL-1β, and the eosinophil recruiting chemokine, CCL11, by quantitative reverse-transcriptase PCR. The expression levels of these genes in the infected group were significantly lower than those of the non-infected group at 48 hours post-infection. Although the immunomodulating ability of the excretory-secretory products released from tetrathyridium has been previously shown by in vitro assays, the significance of this ability in their lifecycle has remained unclear. In this study, we discovered that tetrathyridium causes temporal inflammation in the intestinal wall during penetration and large-scale migration in this organ, but tetrathyridium simultaneously suppresses the host's inflammatory gene expression, might to be a strategy that reduces inflammatory responses and increases survival of the parasite.

Peritoneal cavity

CCL11

Intestinal Disorder

Intestinal mucosa

10.1371/journal.pntd.0008685

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