Miguel Cisneros

Red de Investigación en Actividades Preventivas y Promoción de la Salud

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Patricia Joseph‐Bravo

Universidad Nacional Autónoma de México

Jean‐Louis Charli

Universidad Nacional Autónoma de México

Rosa María Uribe

Universidad Nacional Autónoma de México

Jean-Louis Charli

Universidad Nacional Autónoma de México

Patricia de Gortari

Instituto Nacional de Psiquiatría

Carmen Muñoz‐Almagro

Hospital Sant Joan de Déu Barcelona

Milagros Méndez

Instituto Nacional de Psiquiatría

MÁ

Miguel Ángel Vargas

Universidad Nacional Autónoma de México

Elza Barzdina

Children's Clinical University Hospital

Ana Milagro

Hospital Universitario Miguel Servet

Cooperative Institutions

University of Sydney

120

University of Bari Aldo Moro

110

Universidad Nacional Autónoma de México

Academic Center for Dentistry Amsterdam

University of Havana

Instituto de Salud Carlos III

Istituti di Ricovero e Cura a Carattere Scientifico

Inserm

Instituto Nacional de Psiquiatría

Hospital Sant Joan de Déu Barcelona

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Regional distribution of the membrane-bound pyroglutamate amino peptidase-degrading thyrotropin-releasing hormone in rat brain

Neuroscience Letters (1987)

Miguel Arenas Vargas Milagros Méndez Miguel Cisneros Patricia Joseph‐Bravo Jean Louis Charli

10.1016/0304-3940(87)90450-2

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Citations (52)

Influence of thyroid status on TRH metabolism in rat olfactory bulb

Peptides (1994)

Rosa María Uribe Patricia Joseph‐Bravo Georgina Ponce Miguel Cisneros Carmen Aceves

Hypothalamic–pituitary–thyroid axis

10.1016/0196-9781(94)90201-1

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Citations (4)

Differential Responses of Thyrotropin-Releasing Hormone (TRH) Neurons to Cold Exposure or Suckling Indicate Functional Heterogeneity of the TRH System in the Paraventricular Nucleus of the Rat Hypothalamus

Neuroendocrinology (2001)

Edith Sánchez Rosa María Uribe Gabriel Corkidi R. Thomas Zoeller Miguel Cisneros

Thyrotropin-releasing hormone (TRH) is released from the median eminence upon neural stimulation such as cold or suckling exposure. Concomitant with the cold- or suckling-induced release of TRH is a rapid and transient increase in the expression of proTRH mRNA in the paraventricular nucleus (PVN) of the hypothalamus. We employed two strategies to determine whether TRH neurons responding to cold exposure are different from those responding to suckling. First, we attempted to identify a marker of cellular activation in TRH neurons of the PVN. Cold induced c-fos expression in about 25% of TRH neurons of the PVN, but no induction was observed by suckling. Moreover, we explored the expression of a variety of immediate early genes including NGFI-A, fra-1 and c-jun, or CREB phosphorylation but found none to be induced by suckling. The number of cells expressing high levels of proTRH mRNA was counted and compared to total expressing cells. An increased number of cells expressing high levels of proTRH mRNA was observed when both stimuli were applied to the same animal, suggesting that different cells respond separately to each stimulus. We therefore analyzed the distribution of responsive TRH neurons as defined by the cellular level of proTRH mRNA. The proTRH mRNA signal was analyzed within three rostrocaudal zones of the PVN and within six mediolateral columns. Results showed that in response to cold, all areas of the PVN of the lactating rat present increased proTRH mRNA levels, including the anterior zone where few hypophysiotropic TRHergic cells are believed to reside. The distribution of the proTRH mRNA expressing cells in response to cold was quite comparable in female and in male rats. In contrast, the response after suckling was confined to the middle and caudal zones. Our results provide evidence of a functional specialization of TRH cells in the PVN.

Median eminence

10.1159/000054707

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Citations (74)

Bioquímica celular de la neurona peptidérgica (II) : regulación del metabolismo de neuropéptidos

Luis Covarrubias Jean‐Louis Charli Rosa María Uribe Georgina Ponce Milagros Méndez

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Voluntary Exercise Adapts the Hypothalamus-Pituitary-Thyroid Axis in Male Rats

Endocrinology (2014)

Rosa María Uribe Lorraine Jaimes-Hoy Candy Elizabeth Ramírez-Martínez Arlene García-Vázquez Fidelia Romero

The hypothalamic-pituitary thyroid (HPT) axis modulates energy homeostasis. Its activity decreases in conditions of negative energy balance but the effects of chronic exercise on the axis are controversial and unknown at hypothalamic level. Wistar male rats were exposed for up to 14 days to voluntary wheel running (WR), or pair-feeding (PF; 18% food restriction), or to repeated restraint (RR), a mild stressor. WR and RR diminished food intake; body weight gain decreased in the 3 experimental groups, but WAT mass and serum leptin more intensely in the WR group. WR, but not RR, produced a delayed inhibition of central markers of HPT axis activity. At day 14, in WR rats paraventricular nucleus-pro-TRH mRNA and serum TSH levels decreased, anterior pituitary TRH-receptor 1 mRNA levels increased, but serum thyroid hormone levels were unaltered, which is consistent with decreased secretion of TRH and clearance of thyroid hormones. A similar pattern was observed if WR animals were euthanized during their activity phase. In contrast, in PF animals the profound drop of HPT axis activity included decreased serum T3 levels and hepatic deiodinase 1 activity; these changes were correlated with an intense increase in serum corticosterone levels. WR effects on HPT axis were not associated with changes in the activity of the hypothalamic-pituitary adrenal axis, but correlated positively with serum leptin levels. These data demonstrate that voluntary WR adapts the status of the HPT axis, through pathways that are distinct from those observed during food restriction or repeated stress.

Hypothalamic–pituitary–thyroid axis

Corticosterone

Energy homeostasis

Hypothalamic–pituitary–adrenal axis

10.1210/en.2013-1724

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Citations (36)

Marine organisms : a source of biomedically relevant metallo M1, M2 and M17 exopeptidase inhibitors

HAL (Le Centre pour la Communication Scientifique Directe) (2020)

Isel Pascual Alonso Laura Rivera Méndez Fabiola Almeida García Mario E. Valdés‐Tresanco Yarini Arrebola Sánchez

Proteolytic enzymes, known as peptidases or proteases, are critical in all living organisms. They can act as exo- and/or endo-peptidases. Peptidases are segregated in classes that strongly depend on the chemical nature of the groups involved in catalysis. Peptidases control the activation, synthesis, and turnover of proteins and regulate most biochemical and physiological processes. They are consequently major regulators of homeostasis, ageing, diseases, and death. Proteases are also essential for propagation of infectious agents, being major contributors of pathogenesis in several infectious diseases, including the current coronavirus emergent pandemic COVID-19. Exopeptidases catalyze the cleavage of the N-terminal or C-terminal amino acids of proteins or peptide substrates. They are distributed in many phylla and play critical roles in physiology and pathophysiology. Most of them are metallo peptidases belonging to the M1, M2, and M17 families, among others. Some, such as M1 aminopeptidases N, A and thyrotropin-releasing hormone degrading ectoenzyme, M2 angiotensin converting enzyme and M17 leucyl aminopeptidase are targets for the development of therapeutic agents for human diseases including cancer, hypertension, central nervous system disorders, inflammation, immune system disorders, skin pathologies and infectious diseases, like malaria and coronavirus-induced syndromes. The relevance of exopeptidases has driven the search and identification of potent and selective inhibitors, as major tools to control proteolysis with impact in biochemistry, biotechnology, and biomedicine. The present contribution focuses on marine biodiversity as an important and promising source of inhibitors of metallo exopeptidases from different families, with biomedical applications in human diseases.

Exopeptidase

Proteolysis

Deubiquitinating enzyme

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Citations (1)

Purification of a specific inhibitor of pyroglutamyl aminopeptidase II from the marine annelide Hermodice carunculata

The International Journal of Biochemistry & Cell Biology (2003)

Isel Pascual Shirley Gil-Parrado Miguel Cisneros Patricia Joseph‐Bravo Joaquı́n Dı́az

10.1016/s1357-2725(03)00175-4

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Citations (21)

Regional distribution of in vitro release of thyrotropin releasing hormone in rat brain

Peptides (1987)

Milagros Méndez Patricia Joseph‐Bravo Miguel Cisneros Miguel Ángel Vargas Jean‐Louis Charli

Thyrotropin-releasing hormone receptor

10.1016/0196-9781(87)90104-5

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Citations (35)

Effect of Fasting on the Content of Thyrotropin-releasing Hormone and its mRNA in the Central Nervous System and Pyroglutamyl Peptidase II Activity in the Anterior Pituitary of Post-Weaned and Adult Rats

Nutritional Neuroscience (2000)

Patricia de Gortari Ma. Elena González-Alzati Miguel Cisneros Patricia Joseph‐Bravo

Fasting and malnutrition affect the hypothalamus-pituitary-thyroid axis causing secondary hypothyroidism. The aim of this study was to determine the effect of 48h fasting in rats of 4 and 12 weeks old, and of 7 weeks of food restriction, in the activity of adenohypophysial thyrotropin-releasing hormone (TRH)-degrading ectoenzyme; also, to study if these conditions altered peptide levels and its messenger ribonucleic acid (mRNA) in brain areas of the limbic system. Male Wistar rat brains were excised, frozen and dissected for measurement of: (TRH) content by radioimmunoassay, its mRNA by reverse transcriptase-polymerase chain reaction (RT-PCR), the activity of its degrading ectoenzyme, pyroglutamyl peptidase II, by a fluorometric assay, serum thyrotropin and corticosterone by radioimmunoassay. Malnutrition increased median eminence TRH and decreased thyrotropin serum levels as did fasting in adults, that also diminished hypothalamic peptide content; post-weaned rats in contrast, only had a decreased pyroglutamyl peptidase II activity in adenohypophysis. Serum corticosterone levels increased in all treatments. Brain TRH content varied differently among groups: a decrease in nucleus (n.) accumbens and an increment in hippocampus of 4 weeks old; a decrease in amygdala of adults (with a concomitant diminution in its mRNA levels). No variations were observed in food restricted rats. These results show a differential regulation of TRH metabolism by fasting dependent on the age of the animals.

Corticosterone

10.1080/1028415x.2000.11747323

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Citations (11)

Chronic ethanol or glucose consumption alter TRH content and pyroglutamyl aminopeptidase II activity in rat limbic regions

Regulatory Peptides (2004)

Patricia de Gortari Miguel Cisneros Patricia Joseph‐Bravo

Corticosterone

10.1016/j.regpep.2004.11.002

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Citations (6)