Yu-Hsuan Cheng

National Chung Hsing University

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Jing-Yi Siao

National Yang Ming Chiao Tung University

Chung-Te Chang

Max Planck Institute for Developmental Biology

Ting-Wen Chen

National Yang Ming Chiao Tung University

Michelle Noble

European Molecular Biology Laboratory

Hsiao‐Wei Liao

National Yang Ming Chiao Tung University

Wei-Chung Chiang

National Yang Ming Chiao Tung University

Yi-Tzu Lo

Baylor College of Medicine

Fabian Pease

Stanford University

P. Pianetta

SLAC National Accelerator Laboratory

Cheng‐Kuan Su

National Chung Hsing University

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National Yang Ming Chiao Tung University

Stanford University

Stanford Medicine

Purdue University West Lafayette

National Taipei University

Max Planck Institute for Developmental Biology

Palo Alto University

Institute of Bioinformatics

Applied Materials (United States)

National Institute of Standards and Technology

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A cesium bromide photocathode excited by 405 nm radiation

Applied Physics Letters (2014)

José R. Maldonado Yu-Hsuan Cheng P. Pianetta Fabian Pease L. Hesselink

In several applications, such as electron beam lithography and X-ray differential phase contrast imaging, there is a need for a free electron source with a current density at least 10 A/cm2 yet can be shaped with a resolution down to 20 nm and pulsed. Additional requirements are that the source must operate in a practical demountable vacuum (>1e-9 Torr) and be reasonably compact. In prior work, a photocathode comprising a film of CsBr on metal film on a sapphire substrate met the requirements except it was bulky because it required a beam (>10 W/cm2) of 257 nm radiation. Here, we describe an approach using a 405 nm laser which is far less bulky. The 405 nm laser, however, is not energetic enough to create color centers in CsBr films. The key to our approach is to bombard the CsBr film with a flood beam of about 1 keV electrons prior to operation. Photoelectron efficiencies in the range of 100–1000 nA/mW were demonstrated with lifetimes exceeding 50 h between electron bombardments. We suspect that the electron bombardment creates intraband color centers whence electrons can be excited by the 405 nm photons into the conduction band and thence into the vacuum.

Photocathode

10.1063/1.4890538

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Human DCP1 is crucial for mRNA decapping and possesses paralog-specific gene regulating functions

bioRxiv (Cold Spring Harbor Laboratory) (2023)

Ting-Wen Chen Hsiao‐Wei Liao Michelle Noble Jing-Yi Siao Yu-Hsuan Cheng

ABSTRACT The mRNA 5’-cap structure removal by the decapping enzyme DCP2 is a critical step in gene regulation. While DCP2 is the catalytic subunit in the decapping complex, its activity is strongly enhanced by multiple factors, particularly DCP1, which is the major activator in yeast. However, the precise role of DCP1 in metazoans has yet to be fully elucidated. Moreover, in humans, the specific biological functions of the two DCP1 paralogs, DCP1a and DCP1b, remain largely unknown. To investigate the role of human DCP1, we generated cell-lines that were deficient in DCP1a, DCP1b or both to evaluate the importance of DCP1 in the decapping machinery. Our results highlight the importance of human DCP1 in decapping process and show that the EVH1 domain of DCP1 enhances the mRNA-binding affinity of DCP2. Transcriptome and metabolome analyses outline the distinct functions of DCP1a and DCP1b in human cells, regulating specific endogenous mRNA targets and biological processes. Overall, our findings provide insights into the molecular mechanism of human DCP1 in mRNA decapping and shed light on the distinct functions of its paralogs.

P-bodies

10.1101/2023.09.04.556219

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Human DCP1 is crucial for mRNA decapping and possesses paralog-specific gene regulating functions

eLife (2024)

Ting-Wen Chen Hsiao‐Wei Liao Michelle Noble Jing-Yi Siao Yu-Hsuan Cheng

The mRNA 5'-cap structure removal by the decapping enzyme DCP2 is a critical step in gene regulation. While DCP2 is the catalytic subunit in the decapping complex, its activity is strongly enhanced by multiple factors, particularly DCP1, which is the major activator in yeast. However, the precise role of DCP1 in metazoans has yet to be fully elucidated. Moreover, in humans, the specific biological functions of the two DCP1 paralogs, DCP1a and DCP1b, remain largely unknown. To investigate the role of human DCP1, we generated cell lines that were deficient in DCP1a, DCP1b, or both to evaluate the importance of DCP1 in the decapping machinery. Our results highlight the importance of human DCP1 in decapping process and show that the EVH1 domain of DCP1 enhances the mRNA-binding affinity of DCP2. Transcriptome and metabolome analyses outline the distinct functions of DCP1a and DCP1b in human cells, regulating specific endogenous mRNA targets and biological processes. Overall, our findings provide insights into the molecular mechanism of human DCP1 in mRNA decapping and shed light on the distinct functions of its paralogs.

10.7554/elife.94811

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3D-printed thermally expanded monolithic foam for solid-phase extraction of multiple trace metals

Microchimica Acta (2024)

Yu-Hsuan Cheng Cheng‐Kuan Su

TRACE (psycholinguistics)

Solid phase extraction

10.1007/s00604-024-06691-9

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