Lucia F. Alves‐Oliveira

Universidade Vale do Rio Doce

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Co-Authors

Rodrigo Corrêa‐Oliveira

Fundação Oswaldo Cruz

Alda Maria Soares Silveira

Universidade Federal de Juiz de Fora

G. Gazzinelli

Escola Superior de Ciências da Santa Casa de Misericórdia de Vitória

Olindo Assis Martins‐Filho

Fundação Oswaldo Cruz

Luiz Cosme Cotta Malaquias

Universidade Federal de Alfenas

Iramaya R. C. Viana

Fundação Oswaldo Cruz

Paul Hagan

University of Liverpool

Philip T. LoVerde

The University of Texas Health Science Center at San Antonio

Patricia Carneiro‐Santos

University of Glasgow

Giovanni Gazzinelli

Fundação Oswaldo Cruz

Cooperative Institutions

Universidade Federal de Minas Gerais

Fundação Oswaldo Cruz

Universidade Vale do Rio Doce

Universidade Federal de Ouro Preto

Universidade Federal de Juiz de Fora

Instituto Nacional de Ciência e Tecnologia de Doenças Tropicais

Instituto de Ensino e Pesquisa Santa Casa

Grupo Santa Casa de Belo Horizonte

Universidade Federal do Espírito Santo

Universidade Federal Fluminense

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Human schistosomiasis mansoni: intensity of infection differentially affects the production of interleukin-10, interferon-γ and interleukin-13 by soluble egg antigen or adult worm antigen stimulated cultures

Transactions of the Royal Society of Tropical Medicine and Hygiene (2004)

Alda Maria Soares Silveira Giovanni Gazzinelli Lucia F. Alves‐Oliveira Jeffrey M. Bethony Andréa Gazzinelli

The effect of the intensity of infection (eggs per gram faeces, epg) on the production of interferon-gamma (INF-gamma), interleukin (IL)-10 and IL-13 by peripheral blood mononuclear cells (PBMCs) from individuals living in a Schistosoma mansoni-endemic area was evaluated. In vitro stimulation of PBMCs with soluble egg antigen (SEA) resulted in significantly higher secretion levels of IFN-gamma in egg-negative individuals compared with those with an intensity of infection of more than 100 epg. In contrast, the egg-positive group produced significantly higher amounts of IL-10. Levels of IL-13 did not differ significantly between egg-positive and egg-negative groups. These findings suggest that IL-10 is an important cytokine in the control of the T helper cell (Th) type 1 responses during human S. mansoni infection, shifting the immune response from Th0 in egg-negative individuals from an endemic area to a Th2 polarization in chronic infected individuals.

10.1016/j.trstmh.2003.11.009

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Citations (45)

Antibodies reactivity in humanSchistosoma mansonidisease: detection and functional activity

Annals of Tropical Medicine and Parasitology (1988)

Lucia F. Alves‐Oliveira Munir Chamone C.A. Marques B.C.G. Nascimento Paulo Marcos Zech Coelho

The detection and description of the functional activity of anti S. mansoni antibodies, and the relationship between five different antigen preparations, each corresponding to one immunological test for schistosomiasis, are described. This study, using functional assay, suggests that human antibodies detected by skin tests, direct lysis of sheep erythrocytes, complement fixation reaction, indirect immunofluorescent staining and complement-mediated death of schistosomules, show important dissimilarities in reactivities, and it is impossible to ascribe these reactivities to the same class of antibodies.

Complement fixation test

Schistosoma

10.1080/00034983.1988.11812295

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Citations (1)

Evaluation of immunologic profile in patients with nickel sensitivity due to use of fixed orthodontic appliances

American Journal of Orthodontics and Dentofacial Orthopedics (2003)

Marcelo Marigo Darcy Flávio Nouer Marisa Cristina Santos Genelhu Luiz Cosme Cotta Malaquias Virgínia Ramos Pizziolo

Cellular immunity

10.1016/s0889-5406(03)00239-7

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Citations (50)

Characterization of nickel-induced allergic contact stomatitis associated with fixed orthodontic appliances

American Journal of Orthodontics and Dentofacial Orthopedics (2005)

Marisa Cristina Leite Santos Genelhu Marcelo Marigo Lucia F. Alves‐Oliveira Luiz Cosme Cotta Malaquias Ricardo Santiago Gomez

Etiology

Medical History

Inflammatory response

10.1016/j.ajodo.2005.03.002

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Citations (75)

Effect of Chemotherapy with Praziquantel on the Production of Cytokines and Morbidity Associated with Schistosomiasis Mansoni

Antimicrobial Agents and Chemotherapy (2008)

P. Martins-Leite G. Gazzinelli Lucia F. Alves‐Oliveira Andréa Gazzinelli Luiz Cosme Cotta Malaquias

The objective of the present study was to test the hypothesis that treatment of schistosomiasis mansoni with praziquantel can alter significantly the immune response of patients and generate a reversal of the level of fibrosis. Peripheral blood mononuclear cell (PBMC) samples were collected from, and abdominal ultrasound examinations conducted on, volunteers infected with Schistosoma mansoni and living in an area where the disease is endemic, both prior to and one year after treatment with praziquantel. Subjects were classified into groups according to the level of pathology (i.e., absent, incipient, moderate, or severe fibrosis). PBMCs were stimulated with schistosome soluble egg antigens (SEA), and the levels of production of the cytokines gamma interferon (IFN-gamma), tumor necrosis factor alpha, transforming growth factor beta, and interleukin-4 (IL-4), IL-10, and IL-13 were determined. The chemotherapy was effective in reducing morbidity, particularly for individuals presenting with severe fibrosis. When levels of cytokine production in posttreatment PBMC cultures stimulated by SEA were categorized as low or high, significant differences in the distribution of IL-13 levels between groups presenting with or not presenting with fibrosis were established. Comparison of pre- and posttreatment SEA-induced cytokine levels in individuals who had experienced no change in the grade of fibrosis following chemotherapy revealed that the level of IFN-gamma decreased in subjects with fibrosis whereas that of IL-10 decreased in individuals with and without fibrosis. The data suggest that chemotherapy is effective in reducing the morbidity of the disease and that the level of IL-13 may be a useful indicator of the persistence of fibrosis following treatment.

10.1128/aac.00173-08

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Citations (40)

T.43. Degranulation of Circulating Eosinophils in Patients with Schistosomiasis Mansoni

Clinical Immunology (2009)

Andréa Teixeira‐Carvalho Denise Silveira-Lemos Olindo Assis Martins‐Filho Cristina Guatimosim Mauro M. Teixeira

10.1016/j.clim.2009.03.181

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Citations (0)

Familial Resemblance in Humoral Immune Response to Defined and CrudeSchistosoma mansoniAntigens in an Endemic Area in Brazil

The Journal of Infectious Diseases (1999)

Jeffrey M. Bethony Alda Maria Soares Silveira Lucia F. Alves‐Oliveira Aditya Thakur G. Gazzinelli

This study addressed whether the humoral immune response to crude and defined Schistosoma mansoni antigens aggregates within families. The sample included 155 siblings from 42 nuclear families in Brazil. Sera examined by ELISA for antibody isotypes reactive to defined schistosome antigens and crude schistosome antigens (soluble adult worm antigen preparation and soluble egg antigen) demonstrated that there was a difference in sibling-pair correlations between defined and crude S. mansoni antigens. In contrast to the finding with crude antigens, egg-positive sibling pairs showed significant familial resemblance for all IgG subclasses and IgE to adult-stage antigens Smp20.8 and Smp50. Only the IgE and IgG4 isotypes showed familial resemblance to the egg-stage antigen, Smp40. Egg-negative sibling pairs showed significant familial resemblance only for IgE and IgG4 to Smp40. That both the IgE and IgG4 response to defined S. mansoni antigens showed familial resemblance is interesting in light of the converging evidence for the role of IgE and IgG4 in human susceptibility and resistance to reinfection.

10.1086/315069

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Citations (16)

P38 mitogen‐activated protein kinase influence on the production of IL‐10 in human schistosomiasis mansoni

Parasite Immunology (2002)

Patricia Carneiro‐Santos Lucia F. Alves‐Oliveira Rodrigo Corrêa‐Oliveira Paul Hagan

SUMMARY Schistosomiasis mansoni remains a significant public health problem in many parts of the tropics and subtropics. Clinical manifestations range from the asymptomatic intestinal form through to the hepatosplenic form of the disease, a potentially lethal clinical condition in a subsection of the exposed population. In this study, we investigated the mechanisms by which interleukin (IL)‐10 production could be differentially controlled in patients with the intestinal and hepatosplenic forms of the disease, as IL‐10 may play a fundamental role in the development of the hepatosplenic disease state. It is reported that p38 mitogen‐activated protein (MAP) kinase signalling, and in particular p38 MAPK activation, is central to IL‐10 production of cells from patients with schistosomiasis. Furthermore, the difference in the levels of activated p38 MAPK and the activation transcription factor (ATF‐2), may explain the difference in the amount of IL‐10 produced by cells from intestinal and hepatosplenic patients. We suggest that the type of immune response triggered in patients with hepatosplenomegaly could be influenced by the levels of phosphorylated p38 MAPK.

Hepatosplenomegaly

10.1046/j.1365-3024.2002.00487.x

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Citations (10)

Apoptosis: a mechanism of immunoregulation during human schistosomiasis mansoni

Parasite Immunology (2000)

Patricia Carneiro‐Santos Olindo Assis Martins‐Filho Lucia F. Alves‐Oliveira Alda Maria Soares Silveira Pedro Coura‐Filho

People infected with schistosomes may present with a variety of clinical manifestations ranging from the relatively asymptomatic intestinal (INT) form to the hepatointestinal (HI) or hepatosplenic (HS) forms characterized by hepatomegaly and hepatosplenomegaly with severe portal hypertension, respectively. Flow cytometry analyses were used to evaluate the contribution of apoptosis in specific cell populations from schistosomiasis patients to the development of the different clinical forms of the disease. The results showed that cell death induced by combinations of specific antigen and cytokines corresponds with specific clinical presentations. It was shown that soluble egg antigen (SEA) increased the level of apoptosis only in T cells from INT patients. Stimulation with soluble lung worm antigen preparation (SLAP) did not induce significant differences in the levels of apoptosis in T cells from the patients with the different clinical forms of schistosomiasis. These results suggest for the first time that apoptosis plays an important role in the modulation of the anti-SEA response in INT patients.

Hepatosplenomegaly

10.1046/j.1365-3024.2000.00294.x

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Citations (38)

HPLC fractionated soluble egg antigen from Schistosoma mansoni elicits a heterogeneous human immune response

Parasite Immunology (1996)

Rita B. Moyes Lucia F. Alves‐Oliveira Juçara C. Parra Giovanni Gazzinelli Barbara L. Doughty

Peripheral blood mononuclear cells (PBMC) from five chronic schistosomiasis patients, three former patients, a SEA sensitized individual, and normal controls were tested in lymphoblastogenesis assays for their ability to proliferate in response to soluble egg antigen (SEA) and soluble worm antigen preparation (SWAP) from Schistosoma mansoni . Cells from all patients and the SEA sensitized individual gave significantly higher responses than the normal controls when stimulated with SEA and SWAP. However, the chronic patients’ SEA responses were much lower than those of the former patients and the SEA sensitized individual. When cells from the same donors were tested in the in vitro granuloma assay, all produced significant granulomatous responses except the normal controls. Once it was established that all individuals in the study gave significant lymphoproliferative responses and granulomatous reactions, SEA was subjected to HPLC fractionation to identify immunogenic protein components of SEA. HPLC separation yielded 25 major fractions. SEA responses from the sensitized individual and former patients exhibited broad, unregulated responsiveness including fractions with neutral, less charged proteins while the chronic patients demonstrated a more restricted range of responsiveness. SEA‐HPLC fractions 14, 21, and 22 contain the most immunodominant proteins based on cellular proliferation data from reactive individuals tested.

Lymphoproliferative response

10.1046/j.1365-3024.1996.d01-41.x

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Citations (1)