Using immunoblot analysis, we examined the electrophoretic mobility of glycogen synthase from rat skeletal muscle and adipose tissue. Extracts from muscle freeze clamped in situ contained at least three forms of synthase with different electrophoretic mobilities. Extracts from adipose tissue also contained multiple forms but lacked the form with greatest mobility found in the muscle extracts. Phosphorylation at multiple sites of glycogen synthase is known to deactivate the enzyme and retard its electrophoretic mobility in sodium dodecyl sulfate gels. These results suggest that there is very little or no dephosphorylated glycogen synthase in adipose tissue and that phosphorylated forms of glycogen synthase synthesize adipose tissue glycogen. Relative to control, it is known that fasting decreases and refeeding increases glucose incorporation into glycogen in rat epididymal adipose tissue but not skeletal muscle incubated in vitro in the presence of insulin. Fasting did not change the electrophoretic pattern of muscle synthase but decreased the relative amount of adipose tissue forms with greater mobility. Refeeding increased above control the relative amount of adipose tissue synthase with greater mobility. These results indicate that changes in the phosphorylations that retard mobility contribute to the effects of fasting and refeeding on adipose tissue glycogen metabolism.

Muscle tissue

10.1152/ajpendo.1988.254.6.e720

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Elective surgery system strengthening: development, measurement, and validation of the surgical preparedness index across 1632 hospitals in 119 countries

The Lancet (2022)

James Glasbey Tom Abbott Adesoji Ademuyiwa Adewale Adisa Ehab Alameer

The 2015 Lancet Commission on global surgery identified surgery and anaesthesia as indispensable parts of holistic health-care systems. However, COVID-19 exposed the fragility of planned surgical services around the world, which have also been neglected in pandemic recovery planning. This study aimed to develop and validate a novel index to support local elective surgical system strengthening and address growing backlogs.

Preparedness

10.1016/s0140-6736(22)01846-3

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P3.17 EFFECT OF HYPERTENSION ON ARTERIAL STIFFNESS IN GHANAIAN SUBJECTS WITH TYPE 2 DIABETES

Artery Research (2013)

Kwame Yeboah D.A. Antwi Ben Gyan V. Govoni J.K. Cruickshank

10.1016/j.artres.2013.10.104

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P3.15 ARTERIAL STIFFNESS IN NON-HYPERTENSIVE GHANAIAN SUBJECTS WITH TYPE 2 DIABETES

Artery Research (2013)

Kwame Yeboah A.G.B. Amoah D.A. Antwi Ben Gyan V. Govoni

10.1016/j.artres.2013.10.102

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Differential effects of insulin injections and insulin infusions on levels of glycogen in rat adipose tissue

Deleted Journal (2001)

D.A. Antwi

Studies have shown that while injections of insulin cause an increase in fat mass, infusions of insulin increase fat mass. The aim of this paper was to test the hypothesis that if an increase in glycogen is an indicator of an impending increase in adipose mass, then insulin infusions should not increase glycogen, while insulin injections should. Normal rats were treated with insulin (or saline in control group) either by intraperitoneal injection or by tail vein infusion. In agreement with previous evidence, insulin injections increased glycogen in adipose tissue significantly within 12 hours of injection. In contrast to injections, insulin infusions failed to increase glycogen in rat adipose tissue. This report concludes that adipose tissue glycogen is a prelude to an increase in the size of adipose tissue. (Journal of the Ghana Science Association: 2001 3(3): 1-6)

10.4314/jgsa.v3i3.17758

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O114 THE ROLE OF ENDOTHELIAL DYSFUNCTION IN THE PATHOGENESIS OF PREECLAMPSIA IN GHANAIAN WOMEN

International Journal of Gynecology & Obstetrics (2012)

Kwame Adu‐Bonsaffoh D.A. Antwi Samuel A. Obed Ben Gyan

10.1016/s0020-7292(12)60444-5

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Effects of acute eccentric exercise stimulus on muscle injury and adaptation

Emmanuel Frimpong D.A. Antwi Gifty Asare Charles Antwi‐Boasiako B. Dzudzor

Eccentric exercise

Stimulus (psychology)

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