Manon Peltier

École Normale Supérieure de Lyon

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Trends

Author Order

Document Type

Co-Authors

Sophie Pantalacci

École Normale Supérieure de Lyon

Marion Mouginot

Université Claude Bernard Lyon 1

Philippe Veber

Laboratoire de Biométrie et Biologie Evolutive

Marie Sémon

École Normale Supérieure de Lyon

Juan F. Döppler

University of Buenos Aires

Ana Amador

University of Buenos Aires

Ayako Yamaguchi

University of Occupational and Environmental Health Japan

Gabriel B. Mindlin

Fundación Ciencias Exactas y Naturales

Franz Goller

University of Utah

Laurent Guéguen

Laboratoire de Biométrie et Biologie Evolutive

Cooperative Institutions

Centre National de la Recherche Scientifique

Inserm

Clermont Université

Université Clermont Auvergne

Genetique Reproduction and Developpement

Université Claude Bernard Lyon 1

École Normale Supérieure de Lyon

University of Buenos Aires

Consejo Nacional de Investigaciones Científicas y Técnicas

University of Utah

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Field

Comparative transcriptomics in serial organs uncovers early and pan-organ developmental changes associated with organ-specific evolutionary novelty

Research Square (Research Square) (2023)

Sophie Pantalacci Marie Sémon Marion Mouginot Manon Peltier Philippe Veber

Abstract We know that morphogenesis evolves along with final morphologies, but have little integrated understanding of morphogenesis evolution, except for old principles such as recapitulation and heterochronies. To revisit such principles, we monitored the developmental dynamics of mouse and hamster molars by combining transcriptome timeseries and morphological quantifications. The mouse upper molar evolved a new dental plan with two more cusps. They form last in mouse upper molar, recapitulating their appearance in the fossil record, but divergence in transcriptome dynamics is already visible in early stages. Three corresponding early developmental changes, including heterochronies and changes in cell proportions, combine and result in a new developmental trajectory culminating with the late addition of two cusps. The biggest surprise came from the lower molar, which was initially included as an additional control, but whose developmental trajectories evolved as much as upper molar’s. Their transcriptome dynamics markedly co-evolved, including spatio-dynamic aspects of cusp formation which are obviously involved in the new upper molar phenotype. Hence upper molar innovation relies in part on non-specific changes which impact morphogenesis in a concerted manner in both molars, but have little impact on lower molar phenotype. This counter-intuitive observation was confirmed in bat limbs. By bridging concerted transcriptomic evolution with concerted evolution of developmental mechanisms, our study introduces a principle for the evolution of organ-specific morphological innovation, with early and pan-organ developmental changes. This changes our expectations on the underlying genetic evolution, and highlights the important role of developmental drift in one organ to accommodate adaptation in another.

Body plan

Evolutionary developmental biology

Developmental Biology

10.21203/rs.3.rs-3467110/v1

Cite

Citations (0)

Data and code for "Replay of innate vocal patterns during sleep in suboscines"

Zenodo (CERN European Organization for Nuclear Research) (2021)

Juan F. Döppler Manon Peltier Ana Amador Franz Goller Gabriel B. Mindlin

The repository contains data and scripts that support the analysis in a paper under consideration for publication.

Code (set theory)

Sleep

10.5281/zenodo.4599099

Cite

Citations (0)

Two conserved vocal central pattern generators broadly tuned for fast and slow rates generate species-specific vocalizations inXenopusclawed frogs

bioRxiv (Cold Spring Harbor Laboratory) (2023)

Ayako Yamaguchi Manon Peltier

ABSTRACT Across phyla, species-specific vocalizations are used by males to attract females. Functional analyses of the neural circuitry underlying behavior have been difficult, particularly in vertebrates. However, using an ex vivo brain preparation that produces fictive vocalizations, we previously identified anatomically distinct fast and slow central pattern generators (CPGs) that drive the fast and slow clicks of male courtship calls in male African clawed frogs, Xenopus laevis . To gain insight into the evolution of neural circuits underlying courtship calls, we extended this approach to four additional species. Here, we show that although the exact rate and duration of the clicks are unique to each species, fast and slow CPGs identified in male X. laevis are conserved across species. Further, we show that the development of fast CPGs depends on testosterone in a species-specific manner: testosterone facilitates the development of fast CPGs in a species with a courtship call containing fast clicks, but not in a species with a courtship call made entirely of slow clicks. Finally, we showed that, unlike other vestigial neural circuits that remain latent, the fast CPGs are not inherited by all species; rather, they are possessed only by the species that produce fast clicks. The results suggest that species-specific calls of the genus Xenopus have evolved by utilizing conserved fast or slow CPGs that are broadly tuned to generate fast or slow trains of clicks, the development of which appear to be regulated by a strategic expression of testosterone receptors in the brain of each species.

Biological neural network

Courtship display

10.1101/2023.01.27.525835

Cite

Citations (0)

Oral D-Aspartate Treatment Improves Sperm Fertility in Both Young and Adult B6N Mice

Animals (2022)

Marcello Raspa Renata Paoletti Manon Peltier Mohamed Majjouti Michele Protti

D-Aspartate (D-Asp) treatment improved the fertility of young male C57BL/6N mice in vivo revealing a direct role on capacitation, acrosome reaction, and fertility in vitro in young males only. We investigated whether the positive effect of D-Asp on fertility could be extended to adult males and evaluated the efficacy of a 2- or 4-week-treatment in vivo. Therefore, 20 mM sodium D-Asp was supplied in drinking water to males of different ages so that they were 9 or 16 weeks old at the end of the experiments. After sperm freezing, the in vitro fertilization (IVF) rate, the birth rate, hormone levels (luteinizing hormone (LH), epitestosterone, and testosterone), the sperm quality (morphology, abnormalities, motility, and velocity), the capacitation rate, and the acrosome reaction were investigated. Oral D-Asp treatment improves the fertilizing capability in mice regardless of the age of the animals. Importantly, a short D-Asp treatment of 2 weeks in young males elevates sperm parameters to the levels of untreated adult animals. In vivo, D-Asp treatment highly improves sperm quality but not sperm concentration. Therefore, D-Asp plays a beneficial role in mouse male fertility and may be highly relevant for cryorepositories to improve mouse sperm biobanking.

Capacitation

Acrosome reaction

10.3390/ani12111350

Cite

Citations (4)

Epididymis Response Partly Compensates for Spermatozoa Oxidative Defects in snGPx4 and GPx5 Double Mutant Mice

PLoS ONE (2012)

Anaïs Noblanc Manon Peltier Christelle Damon‐Soubeyrand Nicolas Kerchkove Eléonore Chabory

We report here that spermatozoa of mice lacking both the sperm nucleus glutathione peroxidase 4 (snGPx4) and the epididymal glutathione peroxidase 5 (GPx5) activities display sperm nucleus structural abnormalities including delayed and defective nuclear compaction, nuclear instability and DNA damage. We show that to counteract the GPx activity losses, the epididymis of the double KO animals mounted an antioxydant response resulting in a strong increase in the global H(2)O(2)-scavenger activity especially in the cauda epididymis. Quantitative RT-PCR data show that together with the up-regulation of epididymal scavengers (of the thioredoxin/peroxiredoxin system as well as glutathione-S-transferases) the epididymis of double mutant animals increased the expression of several disulfide isomerases in an attempt to recover normal disulfide-bridging activity. Despite these compensatory mechanisms cauda-stored spermatozoa of double mutant animals show high levels of DNA oxidation, increased fragmentation and greater susceptibility to nuclear decondensation. Nevertheless, the enzymatic epididymal salvage response is sufficient to maintain full fertility of double KO males whatever their age, crossed with young WT female mice.

Glutathione reductase

Glutathione disulfide

10.1371/journal.pone.0038565

Cite

Citations (45)

Replay of innate vocal patterns during night sleep in suboscines

Proceedings of the Royal Society B Biological Sciences (2021)

Juan F. Döppler Manon Peltier Ana Amador Franz Goller Gabriel B. Mindlin

Activation of forebrain circuitry during sleep has been variably characterized as ‘pre- or replay’ and has been linked to memory consolidation. The evolutionary origins of this mechanism, however, are unknown. Sleep activation of the sensorimotor pathways of learned birdsong is a particularly useful model system because the muscles controlling the vocal organ are activated, revealing syringeal activity patterns for direct comparison with those of daytime vocal activity. Here, we show that suboscine birds, which develop their species-typical songs innately without the elaborate forebrain–thalamic circuitry of the vocal learning taxa, also engage in replay during sleep. In two tyrannid species, the characteristic syringeal activation patterns of the song could also be identified during sleep. Similar to song-learning oscines, the burst structure was more variable during sleep than daytime song production. In kiskadees ( Pitangus sulphuratus ), a second vocalization, which is part of a multi-modal display, was also replayed during sleep along with one component of the visual display. These data show unambiguously that variable ‘replay’ of stereotyped vocal motor programmes is not restricted to programmes confined within forebrain circuitry. The proposed effects on vocal motor programme maintenance are, therefore, building on a pre-existing neural mechanism that predates the evolution of learned vocal motor behaviour.

Forebrain

Vocal Learning

Sleep

10.1098/rspb.2021.0610

Cite

Citations (6)

Two conserved vocal central pattern generators broadly tuned for fast and slow rates generate species-specific vocalizations in Xenopus clawed frogs

eLife (2023)

Ayako Yamaguchi Manon Peltier

Across phyla, males often produce species-specific vocalizations to attract females. Although understanding the neural mechanisms underlying behavior has been challenging in vertebrates, we previously identified two anatomically distinct central pattern generators (CPGs) that drive the fast and slow clicks of male Xenopus laevis, using an ex vivo preparation that produces fictive vocalizations . Here, we extended this approach to four additional species, X. amieti, X. cliivi, X. petersii, and X. tropicalis, by developing ex vivo brain preparation from which fictive vocalizations are elicited in response to a chemical or electrical stimulus. We found that even though the courtship calls are species-specific, the CPGs used to generate clicks are conserved across species. The fast CPGs, which critically rely on reciprocal connections between the parabrachial nucleus and the nucleus ambiguus, are conserved among fast-click species, and slow CPGs are shared among slow-click species. In addition, our results suggest that testosterone plays a role in organizing fast CPGs in fast-click species, but not in slow-click species. Moreover, fast CPGs are not inherited by all species but monopolized by fast-click species. The results suggest that species-specific calls of the genus Xenopus have evolved by utilizing conserved slow and/or fast CPGs inherited by each species.

10.7554/elife.86299

Cite

Citations (3)

Phenotypic innovation in one tooth induced concerted developmental evolution in another

bioRxiv (Cold Spring Harbor Laboratory) (2020)

Marie Sémon Klára Steklíková Marion Mouginot Manon Peltier Philippe Veber

ABSTRACT Serial appendages are similar organs found at different places in the body, such as fore/hindlimbs or different teeth. They are bound to develop with the same pleiotropic genes, apart from identity genes. These identity genes have logically been implicated in cases where a single appendage evolved a drastically new shape while the other retained an ancestral shape, by enabling developmental changes specifically in one organ. Here, we showed that independent evolution involved developmental changes happening in both organs, in two well characterized model systems. Mouse upper molars evolved a new dental plan with two more cusps on the lingual side, while the lower molar kept a much more ancestral morphology, as did the molars of hamster, our control species. We obtained quantitative timelines of cusp formation and corresponding transcriptomic timeseries in the 4 molars. We found that a molecular and morphogenetic identity of lower and upper molars predated the mouse and hamster divergence and likely facilitated the independent evolution of molar’s lingual side in the mouse lineage. We found 3 morphogenetic changes which could combine to cause the supplementary cusps in the upper molar and a candidate gene, Bmper . Unexpectedly given its milder morphological divergence, we observed extensive changes in mouse lower molar development. Its transcriptomic profiles diverged as much as, and co-evolved extensively with, those of the upper molar. Consistent with the transcriptomic quantifications, two out of the three morphogenetic changes also impacted lower molar development. Moving to limbs, we show the drastic evolution of the bat wing also involved gene expression co-evolution and a combination of specific and pleiotropic changes. Independent morphological innovation in one organ therefore involves concerted developmental evolution of the other organ. This is facilitated by evolutionary flexibility of its development, a phenomenon known as Developmental System Drift. AUTHOR SUMMARY Serial organs, such as the different wings of an insect or the different limbs or teeth of a vertebrate, can develop into drastically different shapes due to the position-specific expression of so-called “identity” genes. Often during evolution, one organ evolves a new shape while another retains a conserved shape. It was thought that identity genes were responsible for these cases of independent evolution, by enabling developmental changes specifically in one organ. Here, we showed that developmental changes evolved in both organs to enable the independent evolution of the upper molar in mice and the wing in bats. In the organ with the new shape, several developmental changes combine. In the organ with the conserved shape, part of these developmental changes are seen as well. This modifies the development but is not sufficient to drastically change the phenotype, a phenomenon known as “Developmental System Drift”, DSD. Thus, the independent evolution of one organ relies on concerted molecular changes, which will contribute to adaptation in one organ and be no more than DSD in another organ. This concerted evolution could apply more generally to very different body parts and explain previous observations on gene expression evolution.

Body plan

Lineage (genetic)

10.1101/2020.04.22.043422

Cite

Citations (4)

Data and code for "Replay of innate vocal patterns during sleep in suboscines"

Zenodo (CERN European Organization for Nuclear Research) (2021)

Juan F. Döppler Manon Peltier Ana Amador Franz Goller Gabriel B. Mindlin

The repository contains data and scripts that support the analysis in a paper under consideration for publication.

Sleep

Code (set theory)

10.5281/zenodo.4599098

Cite

Citations (0)

Correction: Epididymis Response Partly Compensates for Spermatozoa Oxidative Defects in snGPx4 and GPx5 Double Mutant Mice

PLoS ONE (2012)

We report here that spermatozoa of mice lacking both the sperm nucleaus glutathione peroxidase 4 (snGPx4) and the epididymal glutathione peroxidase 5 (GPx5) activities display sperm nucleus structural abnormalities including delayed and defective nuclear compaction, nuclear instability and DNA damage.We show that to counteract the GPx activity losses, the epididymis of the double KO animals mounted an antioxydant response resulting in a strong increase in the global H 2 O 2scavenger activity especially in the cauda epididymis.Quantitative RT-PCR data show that together with the up-regulation of epididymal scavengers (of the thioredoxin/peroxiredoxin system as well as glutathione-S-transferases) the epididymis of double mutant animals increased the expression of several disulfide isomerases in an attempt to recover normal disulfidebridging activity.Despite these compensatory mechanisms cauda-stored spermatozoa of double mutant animals show high levels of DNA oxidation, increased fragmentation and greater susceptibility to nuclear decondensation.Nevertheless, the enzymatic epididymal salvage response is sufficient to maintain full fertility of double KO males whatever their age, crossed with young WT female mice.

10.1371/annotation/a546c716-7c58-4b69-a19f-62629cb72693

Cite

Citations (10)